{"id":11193,"date":"2023-04-20T14:02:02","date_gmt":"2023-04-20T19:02:02","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?page_id=11193"},"modified":"2023-04-20T22:22:40","modified_gmt":"2023-04-21T03:22:40","slug":"que-es-rho1d4","status":"publish","type":"page","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/que-es-rho1d4\/","title":{"rendered":"\u00bfQu\u00e9 es Rho1D4?"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"11193\" class=\"elementor elementor-11193\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2062367 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2062367\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-a6c3401\" data-id=\"a6c3401\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-debe197 elementor-widget elementor-widget-text-editor\" data-id=\"debe197\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p>Es una etiqueta de afinidad especializada para prote\u00ednas de membrana.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b98f9f0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b98f9f0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-b96b246\" data-id=\"b96b246\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c3abc4a elementor-widget elementor-widget-spacer\" data-id=\"c3abc4a\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-column .elementor-spacer-inner{height:var(--spacer-size)}.e-container{--container-widget-width:100%}.e-container>.elementor-widget-spacer{width:var(--container-widget-width,var(--spacer-size));-ms-flex-item-align:stretch;align-self:stretch;-ms-flex-negative:0;flex-shrink:0}.e-container>.elementor-widget-spacer>.elementor-widget-container,.e-container>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer{height:100%}.e-container>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer>.elementor-spacer-inner{height:var(--container-widget-height,var(--spacer-size))}<\/style>\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2a2c5b1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2a2c5b1\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f888d16\" data-id=\"f888d16\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3f969c8 elementor-widget elementor-widget-image\" data-id=\"3f969c8\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"596\" height=\"344\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Rho1D4-Tag-an-Membrane-Protein.jpg\" class=\"attachment-classic-grid-listing size-classic-grid-listing\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Rho1D4-Tag-an-Membrane-Protein.jpg 596w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Rho1D4-Tag-an-Membrane-Protein-300x173.jpg 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Rho1D4-Tag-an-Membrane-Protein-277x160.jpg 277w\" sizes=\"(max-width: 596px) 100vw, 596px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-26b4fa9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"26b4fa9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ed8d697\" data-id=\"ed8d697\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2236124 elementor-widget elementor-widget-text-editor\" data-id=\"2236124\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>\u00bfQu\u00e9 es la etiqueta Rho1D4?<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-aa4ee31 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"aa4ee31\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-57379a4\" data-id=\"57379a4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a34fb14 elementor-widget elementor-widget-text-editor\" data-id=\"a34fb14\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Las etiquetas de ep\u00edtopos de afinidad de prote\u00ednas encuentran uso en numerosas aplicaciones en ciencias de la vida. Desde la detecci\u00f3n de Western Blot, la localizaci\u00f3n de prote\u00ednas, los ensayos ELISA y, lo que es m\u00e1s importante, la purificaci\u00f3n de prote\u00ednas. Los posibles usos de las etiquetas de ep\u00edtopos son numerosos (Molday &amp; Molday 2014).<\/p><p>Rho1D4 o, a veces, tambi\u00e9n llamado simplemente &#8220;etiqueta 1D4&#8221; es una etiqueta de ep\u00edtopo que se origina en el extremo C-terminal de la rodopsina bovina. Su secuencia larga de nueve amino\u00e1cidos es TETSQVAPA, lo que le da un tama\u00f1o de 902,9 Dalton. La etiqueta de afinidad y los anticuerpos monoclonales espec\u00edficos fueron descubiertos en 1984 por Hodges et al. y Mackenzie et al.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-febf28d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"febf28d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-8e5a818\" data-id=\"8e5a818\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c48e418 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"c48e418\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-divider{--divider-border-style:none;--divider-border-width:1px;--divider-color:#2c2c2c;--divider-icon-size:20px;--divider-element-spacing:10px;--divider-pattern-height:24px;--divider-pattern-size:20px;--divider-pattern-url:none;--divider-pattern-repeat:repeat-x}.elementor-widget-divider .elementor-divider{display:-webkit-box;display:-ms-flexbox;display:flex}.elementor-widget-divider .elementor-divider__text{font-size:15px;line-height:1;max-width:95%}.elementor-widget-divider .elementor-divider__element{margin:0 var(--divider-element-spacing);-ms-flex-negative:0;flex-shrink:0}.elementor-widget-divider .elementor-icon{font-size:var(--divider-icon-size)}.elementor-widget-divider .elementor-divider-separator{display:-webkit-box;display:-ms-flexbox;display:flex;margin:0;direction:ltr}.elementor-widget-divider--view-line_icon .elementor-divider-separator,.elementor-widget-divider--view-line_text .elementor-divider-separator{-webkit-box-align:center;-ms-flex-align:center;align-items:center}.elementor-widget-divider--view-line_icon .elementor-divider-separator:after,.elementor-widget-divider--view-line_icon .elementor-divider-separator:before,.elementor-widget-divider--view-line_text .elementor-divider-separator:after,.elementor-widget-divider--view-line_text .elementor-divider-separator:before{display:block;content:\"\";border-bottom:0;-webkit-box-flex:1;-ms-flex-positive:1;flex-grow:1;border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--element-align-left .elementor-divider .elementor-divider-separator>.elementor-divider__svg:first-of-type{-webkit-box-flex:0;-ms-flex-positive:0;flex-grow:0;-ms-flex-negative:100;flex-shrink:100}.elementor-widget-divider--element-align-left .elementor-divider-separator:before{content:none}.elementor-widget-divider--element-align-left .elementor-divider__element{margin-left:0}.elementor-widget-divider--element-align-right .elementor-divider .elementor-divider-separator>.elementor-divider__svg:last-of-type{-webkit-box-flex:0;-ms-flex-positive:0;flex-grow:0;-ms-flex-negative:100;flex-shrink:100}.elementor-widget-divider--element-align-right .elementor-divider-separator:after{content:none}.elementor-widget-divider--element-align-right .elementor-divider__element{margin-right:0}.elementor-widget-divider:not(.elementor-widget-divider--view-line_text):not(.elementor-widget-divider--view-line_icon) .elementor-divider-separator{border-top:var(--divider-border-width) var(--divider-border-style) var(--divider-color)}.elementor-widget-divider--separator-type-pattern{--divider-border-style:none}.elementor-widget-divider--separator-type-pattern.elementor-widget-divider--view-line .elementor-divider-separator,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:after,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:before,.elementor-widget-divider--separator-type-pattern:not([class*=elementor-widget-divider--view]) .elementor-divider-separator{width:100%;min-height:var(--divider-pattern-height);-webkit-mask-size:var(--divider-pattern-size) 100%;mask-size:var(--divider-pattern-size) 100%;-webkit-mask-repeat:var(--divider-pattern-repeat);mask-repeat:var(--divider-pattern-repeat);background-color:var(--divider-color);-webkit-mask-image:var(--divider-pattern-url);mask-image:var(--divider-pattern-url)}.elementor-widget-divider--no-spacing{--divider-pattern-size:auto}.elementor-widget-divider--bg-round{--divider-pattern-repeat:round}.rtl .elementor-widget-divider .elementor-divider__text{direction:rtl}.e-container>.elementor-widget-divider{width:var(--container-widget-width,100%);--flex-grow:var(--container-widget-flex-grow,0)}<\/style>\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c91dc8b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c91dc8b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-295ce10\" data-id=\"295ce10\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-68feac3 elementor-widget elementor-widget-text-editor\" data-id=\"68feac3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>\u00bfCu\u00e1les son las ventajas de la etiqueta Rho1D4 para la purificaci\u00f3n de prote\u00ednas?<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-24b7967 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"24b7967\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-0cf70ef\" data-id=\"0cf70ef\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9802b4f elementor-widget elementor-widget-text-editor\" data-id=\"9802b4f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Al elegir una etiqueta de afinidad\/ep\u00edtopo para la purificaci\u00f3n de prote\u00ednas, la tarea se reduce a dos preguntas:<br \/>&#8211; \u00bfNecesito m\u00e1s prote\u00edna o m\u00e1s pureza?<br \/>&#8211; \u00bfEsta etiqueta de afinidad influye en la funci\u00f3n de la prote\u00edna?<\/p><p>La etiqueta Rho1D4 est\u00e1 destinada a la m\u00e1xima pureza de prote\u00ednas. Su rendimiento proteico es comparativamente bajo. Consulte esta descripci\u00f3n general de las diferentes <a href=\"https:\/\/cube-biotech.com\/affinity-chromatography-the-principles#tagcomparison\" target=\"_blank\" rel=\"noopener\">etiquetas de ep\u00edtopos<\/a> para ver d\u00f3nde se encuentran algunas de ellas en esta escala de equilibrio.<\/p><p>Por lo tanto, recomendamos usar la etiqueta Rho1D4, especialmente para la purificaci\u00f3n de prote\u00ednas con poca abundancia. Dado que estas prote\u00ednas deben purificarse de una magnitud de otras prote\u00ednas. Por lo tanto, la especificidad de la perla de purificaci\u00f3n para la etiqueta de afinidad debe ser lo m\u00e1s alta posible para evitar uniones inespec\u00edficas. Algo a lo que tiende una purificaci\u00f3n basada en etiquetas His. La uni\u00f3n de los <a href=\"https:\/\/cube-biotech.com\/products\/protein-purification-products\/rho1d4-tag-affinity-resins-magbeads\/rho1d4-antibody-0.2-mg\/40020\" target=\"_blank\" rel=\"noopener\">anticuerpos monoclonales Rho1D4<\/a> a las perlas es la raz\u00f3n de esta especificidad. Esta posibilidad de m\u00e1xima pureza proteica convierte a la etiqueta Rho1D4 en la mejor opci\u00f3n para purificar todo un grupo de prote\u00ednas de baja abundancia: las prote\u00ednas de membrana.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-f73f5b1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"f73f5b1\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-eb4e025\" data-id=\"eb4e025\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ac83281 elementor-widget elementor-widget-text-editor\" data-id=\"ac83281\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Rho1D4 como etiqueta de afinidad para prote\u00ednas de membrana<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fd7fed0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fd7fed0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-85f97c4\" data-id=\"85f97c4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fe3d7ba elementor-widget elementor-widget-text-editor\" data-id=\"fe3d7ba\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Es m\u00e1s dif\u00edcil trabajar con prote\u00ednas de membrana que con sus contrapartes solubles. El hecho de que la eliminaci\u00f3n de la membrana celular los deje inestables sin el uso de <a href=\"https:\/\/cube-biotech.com\/Products\/Ultrapure-Detergents\/\" target=\"_blank\" rel=\"noopener\">detergentes<\/a> o <a href=\"https:\/\/cube-biotech.com\/what-are-nanodiscs\" target=\"_blank\" rel=\"noopener\">nanodiscos<\/a> hace que algunos cient\u00edficos se muestren reacios a trabajar con ellos. Debido al hecho adicional de que las etiquetas de afinidad m\u00e1s utilizadas no son adecuadas para la purificaci\u00f3n adecuada de prote\u00ednas de membrana, aumenta a\u00fan m\u00e1s este efecto. Sin embargo, hay estudios y publicaciones que siguieron adelante y aprovecharon las ventajas del sistema Rho1D4 y purificaron con \u00e9xito la prote\u00edna de membrana activa, consulte la tabla 1.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7916af5 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7916af5\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-157a258\" data-id=\"157a258\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a8d3b30 elementor-widget elementor-widget-spacer\" data-id=\"a8d3b30\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-214fb1d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"214fb1d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-cc1c89b\" data-id=\"cc1c89b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-305cb08 elementor-widget elementor-widget-text-editor\" data-id=\"305cb08\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Tabla 1: Uso del sistema Rho1D4 en la literatura<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-182d408 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"182d408\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-cea6095\" data-id=\"cea6095\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8691b96 elementor-widget elementor-widget-text-editor\" data-id=\"8691b96\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<table class=\"responsiv col-12\"><tbody><tr><th>PROTEIN<\/th><th>EXPRESSION SYSTEM<\/th><th>PURITY<\/th><th>YIELD (TOTAL PROTEIN RECOVERED)<\/th><th>PURIFICATION STEPS TAKEN<\/th><\/tr><tr><td><a href=\"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3779079\/\" target=\"_blank\" rel=\"noopener\">hCB2 G protein-coupled receptor<sup>Locatelli-Hoops et al.<\/sup><\/a><\/td><td>E.coli<\/td><td>90%<\/td><td>4.5 mg\/5 L culture<\/td><td>Rho1D4 IAC + IMAC<\/td><\/tr><tr><td><a href=\"https:\/\/www.nature.com\/articles\/srep00172\" target=\"_blank\" rel=\"noopener\">hVN1R1 G protein-coupled receptor<sup>Corin et al.<\/sup><\/a><\/td><td>Inducible HEK293S cell line (mammal)<\/td><td>90%<\/td><td>1 mg\/1 g cells<\/td><td>Rho1D4 IAC + SEC<\/td><\/tr><tr><td><a href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0023076\" target=\"_blank\" rel=\"noopener\">FPR3 G protein-coupled receptor<sup>Wang &amp; Zhang<\/sup><\/a><\/td><td>Inducible HEK293S cell line (mammal)<\/td><td>90%<\/td><td>2 mg\/6 g cells<\/td><td>Rho1D4 IAC + SEC<\/td><\/tr><tr><td><a href=\"https:\/\/www.nature.com\/articles\/srep00102\" target=\"_blank\" rel=\"noopener\">TAAR5 G protein-coupled receptor<sup>Wang et al.<\/sup><\/a><\/td><td>Inducible HEK293S cell line (mammal)<\/td><td>90%<\/td><td>1 mg\/9 g cells<\/td><td>Rho1D4 IAC + SEC<\/td><\/tr><tr><td><a href=\"https:\/\/www.researchgate.net\/publication\/49651839_Study_of_Bioengineered_Zebra_Fish_Olfactory_Receptor_131-2_Receptor_Purification_and_Secondary_Structure_Analysis\" target=\"_blank\" rel=\"noopener\">OR131-2 G protein-coupled receptor<sup>Leck et al.<\/sup><\/a><\/td><td>Inducible HEK293S cell line (mammal)<\/td><td>90%<\/td><td>2.9 mg\/10 g cells<\/td><td>Rho1D4 IAC + centrifugation<\/td><\/tr><tr><td><a href=\"https:\/\/www.researchgate.net\/publication\/23151779_Study_of_a_Synthetic_Human_Olfactory_Receptor_17-4_Expression_and_Purification_from_an_Inducible_Mammalian_Cell_Line\" target=\"_blank\" rel=\"noopener\">hOR17-4 G protein-coupled receptor<sup>Cook et al.<\/sup><\/a><\/td><td>Inducible HEK293S cell line (mammal)<\/td><td>90%<\/td><td>7.5 mg\/2.5 L culture<\/td><td>Rho1D4 IAC + SEC<\/td><\/tr><tr><td><a href=\"https:\/\/www.pnas.org\/content\/106\/29\/11925\" target=\"_blank\" rel=\"noopener\">hOR17-4 G protein-coupled receptor<sup>Cook et al.<\/sup><\/a><\/td><td>Cell-free wheat germ extract<\/td><td>70%*<\/td><td>0.3 mg\/mL reaction solution*<\/td><td>Rho1D4 IAC + SEC<\/td><\/tr><tr><td><a href=\"https:\/\/www.researchgate.net\/publication\/51399889_High-Level_Expression_Single-Step_Immunoaffinity_Purification_and_Characterization_of_Human_Tetraspanin_Membrane_Protein_CD81\" target=\"_blank\" rel=\"noopener\">CD81 Tetraspanin membrane protein<sup>Takayama et al.<\/sup><\/a><\/td><td>Inducible HEK293S cell line (mammal)<\/td><td>&gt;95%<\/td><td>26 ug\/3X10^7 cells<\/td><td>Rho1D4 IAC<\/td><\/tr><tr><td><a href=\"https:\/\/www.researchgate.net\/publication\/45100312_Purification_of_functional_human_Cl-HCO3_-_exchanger_AE1_over-expressed_in_Saccharomyces_cerevisiae\" target=\"_blank\" rel=\"noopener\">AE1 Solute carrier<sup>Bonar &amp; Casey<\/sup><\/a><\/td><td>S. cerevisiae strain BJ5457<\/td><td>93%<\/td><td>2.5 mg\/18 L culture<\/td><td>Rho1D4 IAC<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-009dfec elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"009dfec\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3d61f3e\" data-id=\"3d61f3e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-34f35d1 elementor-widget elementor-widget-text-editor\" data-id=\"34f35d1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Ejemplos de pureza y rendimiento informados en la literatura para prote\u00ednas de membrana purificadas con el sistema rho1D4. Aunque muchas de las prote\u00ednas purificadas con el sistema Rho1D4 han sido receptores acoplados a prote\u00edna G, el sistema tiene la flexibilidad para facilitar la caracterizaci\u00f3n de otras prote\u00ednas de membrana, como los transportadores. Los art\u00edculos de referencia se enumeran en la literatura citada. IAC: cromatograf\u00eda de inmunoafinidad; IMAC: cromatograf\u00eda de afinidad con metales inmovilizados; SEC: cromatograf\u00eda de exclusi\u00f3n por tama\u00f1o.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6958bf1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6958bf1\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-4e0c828\" data-id=\"4e0c828\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ed4cf07 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"ed4cf07\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7b99431 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7b99431\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6d2bc21\" data-id=\"6d2bc21\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-51bd7df elementor-widget elementor-widget-text-editor\" data-id=\"51bd7df\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Caracteristicas de Rho1D4-Tag<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-8d868e6 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8d868e6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-869bf1d\" data-id=\"869bf1d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-56d6910 elementor-widget elementor-widget-text-editor\" data-id=\"56d6910\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Recopilamos los datos m\u00e1s importantes sobre la etiqueta Rho1D4 y sus capacidades para la purificaci\u00f3n de prote\u00ednas.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7178b6c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7178b6c\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-9590fc3\" data-id=\"9590fc3\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9826fb3 elementor-widget elementor-widget-text-editor\" data-id=\"9826fb3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Tabla 2: Aspectos destacados de la etiqueta Rho1D4<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-370d160 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"370d160\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d6568e1\" data-id=\"d6568e1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-caee111 elementor-widget elementor-widget-text-editor\" data-id=\"caee111\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<table class=\"cube_table\"><tbody><tr><th>CATEGORY<\/th><th>FEATURE<\/th><\/tr><tr><td>Sequence (Amino Acids)<\/td><td>9 amino acids: TETSQVAPA<\/td><\/tr><tr><td>Sequence (DNA)<\/td><td>5&#8217;direction: ACC-GAG-ACT-TCC-CAG-GTC-GCG-CCA-GCT<br \/>3&#8217;direction: AGC-TGG-CGC-GAC-CTG-GGA-AGT-CTC-GGT<\/td><\/tr><tr><td>Size of the tag in Da<\/td><td>902.9 Da<\/td><\/tr><tr><td>Affinity matrix<\/td><td><a href=\"https:\/\/cube-biotech.com\/products\/protein-purification-products\/rho1d4-tag-affinity-resins-magbeads\/rho1d4-antibody-0.2-mg\/40020\">Anti-Rho1D4 antibody (approx. 150 kDa)<\/a><\/td><\/tr><tr><td>Elution conditions<\/td><td><a href=\"https:\/\/cube-biotech.com\/products\/protein-purification-products\/rho1d4-tag-affinity-resins-magbeads\/\">Rho1D4 peptide<\/a>, low pH, or tag cleavage<\/td><\/tr><tr><td>Specificity of interaction (KD)<\/td><td>20 nM<\/td><\/tr><tr><td>Typical protein yield per ml resin<\/td><td>3-4 mg\/ml<\/td><\/tr><\/tbody><\/table>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-f092fd4 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"f092fd4\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1d9c94e\" data-id=\"1d9c94e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fb23acd elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"fb23acd\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a37d34f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"a37d34f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-26b0bf4\" data-id=\"26b0bf4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9d079a4 elementor-widget elementor-widget-text-editor\" data-id=\"9d079a4\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>C\u00f3mo agregar\/clonar una etiqueta de ep\u00edtopo Rho1D4<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-8fcfe91 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8fcfe91\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-21abcd7\" data-id=\"21abcd7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-38f77b7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"38f77b7\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-57ada99\" data-id=\"57ada99\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2081cb7 elementor-widget elementor-widget-text-editor\" data-id=\"2081cb7\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>La mejor manera de agregar una etiqueta Rho1D4 a su prote\u00edna de inter\u00e9s es a trav\u00e9s de una estrategia de PCR que agrega la etiqueta Rho1D4 al extremo C-terminal de la prote\u00edna de inter\u00e9s. En Cube Biotech recomendamos una estrategia de clonaci\u00f3n bastante est\u00e1ndar aqu\u00ed, ya que solo es necesario agregar unos pocos nucle\u00f3tidos al gen. Tambi\u00e9n podemos darle un ejemplo de c\u00f3mo se ver\u00edan esos cebadores.<\/p><p><strong>1. Forward Primer:<\/strong> nuestro dise\u00f1o Primer asume que est\u00e1 utilizando un vector de expresi\u00f3n est\u00e1ndar que incluye alguna forma de sitio de clonaci\u00f3n m\u00faltiple (MCS). Del extremo 5&#8242; al 3&#8242; del cebador:<\/p><p>\u00a0 \u2022 Unos pocos nucle\u00f3tidos sobresalientes facilitan la digesti\u00f3n de restricci\u00f3n. Sugerimos 3-4 nts aqu\u00ed, pero en teor\u00eda, podr\u00edan ser tantos como quieras.<br \/>\u00a0 \u2022 El sitio de restricci\u00f3n. Por supuesto, esto depende de su vector de expresi\u00f3n elegido y su MSC espec\u00edfico. Nuestro ejemplo de cartilla usa NdeI.<br \/>\u00a0 \u2022 20-25 nts del N-terminal de su prote\u00edna de inter\u00e9s. Nuestro ejemplo aqu\u00ed usa eGFP.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6b74e19 elementor-widget elementor-widget-text-editor\" data-id=\"6b74e19\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><strong>2. Reverse Primer:<\/strong> el Primer inverso es m\u00e1s largo que el Forward Primer, ya que tiene que incluir m\u00e1s caracter\u00edsticas que este. Del extremo 5&#8242; al 3&#8242; del cebador:<\/p><p>\u00a0 \u2022 Unos pocos nucle\u00f3tidos sobresalientes facilitan la digesti\u00f3n de restricci\u00f3n. Sugerimos 3-4 nts aqu\u00ed, pero en teor\u00eda, podr\u00edan ser tantos como quieras.<br \/>\u00a0 \u2022 El sitio de restricci\u00f3n del Reverse Primer. Depende de su vector de expresi\u00f3n elegido y su MSC espec\u00edfico. Nuestro primer de ejemplo usa XhoI.<br \/>\u00a0 \u2022 Dos codones de parada TCA para garantizar que la prote\u00edna recombinante realmente termine aqu\u00ed<br \/>\u00a0 \u2022 La etiqueta Rho1D4<br \/>\u00a0 \u2022 Opcional: una secuencia espaciadora larga de cuatro amino\u00e1cidos para garantizar la accesibilidad de la etiqueta para las <a href=\"https:\/\/cube-biotech.com\/products\/protein-purification-products\/rho1d4-tag-affinity-resins-magbeads\/\" target=\"_blank\" rel=\"noopener\">perlas de purificaci\u00f3n.<\/a><br \/>\u00a0 \u2022 20-25 nts del extremo 3&#8242; del gen de inter\u00e9s. SIN incluir el cod\u00f3n de parada. Nuestro cebador de ejemplo utiliza la secuencia de reserva de <a href=\"https:\/\/www.ncbi.nlm.nih.gov\/nucleotide\/EU679367.1?report=genbank&amp;log$=nuclalign&amp;blast_rank=100&amp;RID=FVMNGY5M016\" target=\"_blank\" rel=\"noopener\">eGFP<\/a>.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c92cf17 elementor-widget elementor-widget-text-editor\" data-id=\"c92cf17\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"col-12 float-left\">Legend &#8211; Forward primer:<br \/>5&#8242; &#8211;\u00a0<strong>Overhang<\/strong>\u00a0&#8211;\u00a0<span data-mce-mark=\"1\"><span style=\"color: #008000;\">Restriction site \u2b95 NdeI in this case<\/span><\/span>\u00a0&#8211;\u00a0<span style=\"color: #ff6600;\">First 20 nts of eGFP\u00a0<\/span>&#8211; 3&#8242;<br \/>5&#8242; &#8211;\u00a0<span data-mce-mark=\"1\">GGG<\/span><span data-mce-mark=\"1\">\u00a0CATATG\u00a0<\/span><span style=\"color: #ff6600;\">ATGAAACATCACCATCACCA\u00a0<\/span>&#8211; 3&#8242;<\/div><div>\u00a0<\/div><div class=\"col-12 float-left\">Legend &#8211; Reverse primer:<br \/>5&#8242; &#8211;\u00a0<strong>Overhang<\/strong>&#8211;\u00a0<span style=\"color: #ff0000;\">XhoI site\u00a0<\/span>&#8211;\u00a0<span style=\"color: #993366;\" data-mce-mark=\"1\">Stop Codons<\/span>\u00a0&#8211;\u00a0<span style=\"color: #003366;\">Rho1D4-tag\u00a0<\/span>&#8211;\u00a0<span data-mce-mark=\"1\">Spacer sequence\u00a0<em>(optional)<\/em><\/span>\u00a0&#8211;\u00a0<span style=\"color: #ff6600;\" data-mce-mark=\"1\">Last 20 nts of eGFP<\/span>-3&#8242;<br \/>5&#8242;- <span data-mce-mark=\"1\">GGG<\/span>\u00a0<span style=\"color: #ff0000;\" data-mce-mark=\"1\">CTCGAG<\/span>\u00a0<span style=\"color: #993366;\" data-mce-mark=\"1\">TCATCA<\/span>\u00a0<span style=\"color: #003366;\">AGCTGGCGCCACCTGGGAAGTCTCGGT\u00a0<\/span><span style=\"color: #008000;\" data-mce-mark=\"1\">GCCGGAGGAGCC<\/span>\u00a0<span style=\"color: #ff6600;\" data-mce-mark=\"1\">TTTGTATAGTTCATCCATGC<\/span>\u00a0-3&#8242;<\/div>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-9506ee7\" data-id=\"9506ee7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-dd689c8 elementor-widget elementor-widget-image\" data-id=\"dd689c8\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"513\" height=\"886\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Overview-Cloning.jpg\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Overview-Cloning.jpg 513w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Overview-Cloning-174x300.jpg 174w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Overview-Cloning-347x600.jpg 347w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Overview-Cloning-93x160.jpg 93w\" sizes=\"(max-width: 513px) 100vw, 513px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-05618ea elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"05618ea\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1cc7f39\" data-id=\"1cc7f39\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a2c64e2 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"a2c64e2\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-478af21 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"478af21\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-bec5a34\" data-id=\"bec5a34\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-181f5b5 elementor-widget elementor-widget-text-editor\" data-id=\"181f5b5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Purificaci\u00f3n de prote\u00ednas usando la etiqueta Rho1D4<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-90aa855 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"90aa855\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ab42841\" data-id=\"ab42841\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-eec6d09 elementor-widget elementor-widget-text-editor\" data-id=\"eec6d09\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>La purificaci\u00f3n de prote\u00ednas con la etiqueta Rho1D4 pertenece a los m\u00e9todos de cromatograf\u00eda de afinidad. Funciona uniendo el anticuerpo Rho1D4 a resina de agarosa o perlas magn\u00e9ticas. El procedimiento de purificaci\u00f3n se separa en las tres partes siguientes.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9aacd42 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9aacd42\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-017cec7\" data-id=\"017cec7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-a9b808f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"a9b808f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-ef2d232\" data-id=\"ef2d232\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0eabc6e elementor-widget elementor-widget-text-editor\" data-id=\"0eabc6e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><strong>1. Vinculaci\u00f3n:<\/strong><br \/>El primer paso es unir las prote\u00ednas etiquetadas con Rho1D4 a las perlas de resina de agarosa o perlas magn\u00e9ticas. Esto ocurre despu\u00e9s de que los sedimentos celulares que contienen las prote\u00ednas etiquetadas sufrieron lisis celular. Este paso se produce en un tamp\u00f3n de uni\u00f3n Rho especializado. Su composici\u00f3n se puede encontrar aqu\u00ed. Dado que la etiqueta Rho1D4 est\u00e1 destinada a prote\u00ednas de membrana y prote\u00ednas de baja expresi\u00f3n, recomendamos realizar el paso de uni\u00f3n durante varias horas. En el mejor de los casos durante la noche a temperatura ambiente.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-1f4559f\" data-id=\"1f4559f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-beaf0e4 elementor-widget elementor-widget-image\" data-id=\"beaf0e4\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"326\" height=\"298\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step1_bind_wo_legend.jpg\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step1_bind_wo_legend.jpg 326w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step1_bind_wo_legend-300x274.jpg 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step1_bind_wo_legend-175x160.jpg 175w\" sizes=\"(max-width: 326px) 100vw, 326px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7683fc0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7683fc0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-b6e0c11\" data-id=\"b6e0c11\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-2666399 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2666399\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-254984b\" data-id=\"254984b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ba9870a elementor-widget elementor-widget-image\" data-id=\"ba9870a\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"326\" height=\"317\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step2_wash_wo_legend.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step2_wash_wo_legend.png 326w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step2_wash_wo_legend-300x292.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step2_wash_wo_legend-165x160.png 165w\" sizes=\"(max-width: 326px) 100vw, 326px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-f5366e0\" data-id=\"f5366e0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-9afccba elementor-widget elementor-widget-text-editor\" data-id=\"9afccba\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><strong>2. Lavado:<\/strong><br \/>Ahora es el momento de eliminar el resto del lisado celular. Este es el paso de purificaci\u00f3n real en este ensayo de purificaci\u00f3n. Una vez m\u00e1s, aqu\u00ed entra en juego el hecho de que las prote\u00ednas de baja expresi\u00f3n y las prote\u00ednas de membrana son las m\u00e1s adecuadas para el etiquetado de Rho1D4. Debido a la abundancia normalmente baja de prote\u00ednas etiquetadas con Rho1D4, el lavado debe realizarse bien y concienzudamente. Las impurezas restantes debido a la falta de un lavado adecuado pueden superponerse a la se\u00f1al de la prote\u00edna marcada con Rho1D4 debido a su baja abundancia. Esto se evita realizando m\u00e1s pasos de lavado de los m\u00ednimamente recomendados. Al menos 2-3 veces.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-177bc1c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"177bc1c\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-af898d4\" data-id=\"af898d4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-87955f0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"87955f0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-6449da9\" data-id=\"6449da9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-39c8ef6 elementor-widget elementor-widget-text-editor\" data-id=\"39c8ef6\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><strong>3. Eluci\u00f3n:<\/strong><br \/>El paso final de la purificaci\u00f3n se basa en el principio de uni\u00f3n competitiva de mol\u00e9culas a un objetivo de afinidad. En este caso, la uni\u00f3n de las prote\u00ednas marcadas con Rho1D4 se ve desafiada por una cantidad excesiva de p\u00e9ptido Rho1D4. Una magnitud de etiquetas Rho1D4 puras, por as\u00ed decirlo. Estos p\u00e9ptidos se unen primero a los anticuerpos Rho1D4 vac\u00edos que quedan en las perlas. Luego, el p\u00e9ptido comienza a reemplazar la prote\u00edna etiquetada con Rho1D4 en las perlas, esta es la eluci\u00f3n. Por lo tanto, la concentraci\u00f3n del p\u00e9ptido no debe ser demasiado baja. Recomendamos al menos 200 \u00b5mol. La prote\u00edna eluida se recoge dentro de nuestro tamp\u00f3n de eluci\u00f3n y est\u00e1 lista para seguir proces\u00e1ndose en funci\u00f3n de los experimentos planificados.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-931e54f\" data-id=\"931e54f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2daae11 elementor-widget elementor-widget-image\" data-id=\"2daae11\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"326\" height=\"317\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step3_elution_wo_legend.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step3_elution_wo_legend.png 326w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step3_elution_wo_legend-300x292.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Step3_elution_wo_legend-165x160.png 165w\" sizes=\"(max-width: 326px) 100vw, 326px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-32ddc30 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"32ddc30\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-cacd955\" data-id=\"cacd955\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0375162 elementor-widget elementor-widget-text-editor\" data-id=\"0375162\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Nuestra cartera de <a href=\"https:\/\/cube-biotech.com\/products\/protein-purification-products\/rho1d4-tag-affinity-resins-magbeads\/\" target=\"_blank\" rel=\"noopener\">productos con afinidad Rho1D4-tag<\/a> tiene dos protocolos principales que siguen la mayor\u00eda de estos productos. La \u00fanica excepci\u00f3n son los cartuchos, sus protocolos dependen del hardware FPLC utilizado.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-74e31cd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"74e31cd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-13b5097\" data-id=\"13b5097\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-82c2de0 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"82c2de0\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-0bd9a43\" data-id=\"0bd9a43\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-5f13d7c elementor-widget elementor-widget-text-editor\" data-id=\"5f13d7c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><a href=\"https:\/\/cube-biotech.com\/media\/ca\/50\/17\/1666947796\/Rho1D4-tag%20Agarose%20Purification%20Protocol.pdf\" target=\"_blank\" rel=\"noopener\">RHO1D4 AGAROSE RESIN PROTEIN PURIFICATION PROTOCOL<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-inner-column elementor-element elementor-element-1b74391\" data-id=\"1b74391\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-489224a elementor-widget elementor-widget-text-editor\" data-id=\"489224a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><a href=\"https:\/\/cube-biotech.com\/media\/c9\/f4\/b2\/1666947796\/Rho1D4-tag%20MagBeads%20Purification%20Protocol.pdf\" target=\"_blank\" rel=\"noopener\">RHO1D4 MAGBEAD PROTEIN PURIFICATION PROTOCOL<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b120cec elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b120cec\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6537025\" data-id=\"6537025\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2402ce3 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"2402ce3\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-22c49d9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"22c49d9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-15f68fa\" data-id=\"15f68fa\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-da3ddcd elementor-widget elementor-widget-text-editor\" data-id=\"da3ddcd\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Referencias<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-70d46bf elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"70d46bf\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-5a9a9c0\" data-id=\"5a9a9c0\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c0dd3ff elementor-widget elementor-widget-text-editor\" data-id=\"c0dd3ff\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<ol><li>Molday, L. L. &amp; Molday, R. S. 1D4: a versatile epitope tag for the purification and characterization of expressed membrane and soluble proteins.\u00a0<em>Methods Mol Biol<\/em>\u00a0<strong>1177<\/strong>, 1\u201315 (2014).<\/li><li>Hodges, R., Heaton, R., Parker, J. M. R., Molday, L. &amp; Molday, R. Antigen-antibody interaction. Synthetic peptides define linear antigenic determinants recognized by monoclonal antibodies directed to the cytoplasmic carboxyl terminus of rhodopsin.\u00a0<em>The Journal of biological chemistry<\/em>\u00a0<strong>263<\/strong>, 11768\u201375 (1988).<\/li><li>Locatelli-Hoops, S. C., Gorshkova, I., Gawrisch, K. &amp; Yeliseev, A. A. Expression, surface immobilization, and characterization of functional recombinant cannabinoid receptor CB2.\u00a0<em>Biochim Biophys Acta<\/em>\u00a0<strong>1834<\/strong>, 2045\u20132056 (2013).<\/li><li>Takayama, H., Chelikani, P., Reeves, P., Zhang, S. &amp; Khorana, H. High-Level Expression, Single-Step Immunoaffinity Purification and Characterization of Human Tetraspanin Membrane Protein CD81.\u00a0<em>PloS one<\/em>\u00a0<strong>3<\/strong>, e2314 (2008).<\/li><li>Cook, B. L.\u00a0<em>et al.<\/em>\u00a0Large-scale production and study of a synthetic G protein-coupled receptor: Human olfactory receptor 17-4.\u00a0<em>Proc Natl Acad Sci USA<\/em>\u00a0<strong>106<\/strong>, 11925 (2009).<\/li><li>MacKenzie, D., Arendt, A., Hargrave, P., McDowell, J. &amp; Molday, R. Localization of binding sites for carboxyl terminal specific anti-rhodopsin monoclonal antibodies using synthetic peptides.\u00a0<em>Biochemistry<\/em>\u00a0<strong>23<\/strong>, 6544\u20136549 (1984).<\/li><li>Wang, X. &amp; Zhang, S. Production of a Bioengineered G-Protein Coupled Receptor of Human Formyl Peptide Receptor 3.\u00a0<em>PLOS ONE<\/em>\u00a0<strong>6<\/strong>, e23076 (2011).<\/li><li>Bonar, P. &amp; Casey, J. Purification of functional human Cl-\/HCO3 &#8211; exchanger, AE1, over-expressed in Saccharomyces cerevisiae.\u00a0<em>Protein expression and purification<\/em>\u00a0<strong>74<\/strong>, 106\u201315 (2010).<\/li><li>Corin, K.\u00a0<em>et al.<\/em>\u00a0Structure and function analyses of the purified GPCR human vomeronasal type 1 receptor 1.\u00a0<em>Scientific Reports<\/em>\u00a0<strong>1<\/strong>, 172 (2011).<\/li><li>Cook, B., Tourle, K., Chung, H. &amp; Zhang, S. Study of a Synthetic Human Olfactory Receptor 17-4: Expression and Purification from an Inducible Mammalian Cell Line.\u00a0<em>PloS one<\/em>\u00a0<strong>3<\/strong>, e2920 (2008).<\/li><li>Leck, K., Zhang, S. &amp; Hauser, C. Study of Bioengineered Zebra Fish Olfactory Receptor 131-2: Receptor Purification and Secondary Structure Analysis.\u00a0<em>PloS one<\/em>\u00a0<strong>5<\/strong>, e15027 (2010).<\/li><li>Wang, X., Corin, K., Rich, C. &amp; Zhang, S. Study of two G-protein coupled receptor variants of human trace amine-associated receptor 5.\u00a0<em>Scientific Reports<\/em>\u00a0<strong>1<\/strong>, 102 (2011).<\/li><\/ol>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-941f2d6 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"941f2d6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3a62d7a\" data-id=\"3a62d7a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d26696c elementor-widget elementor-widget-spacer\" data-id=\"d26696c\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Es una etiqueta de afinidad especializada para prote\u00ednas de membrana. \u00bfQu\u00e9 es la etiqueta Rho1D4? Las etiquetas de ep\u00edtopos de afinidad de prote\u00ednas encuentran uso en numerosas aplicaciones en ciencias de la vida. Desde la detecci\u00f3n de Western Blot, la localizaci\u00f3n de prote\u00ednas, los ensayos ELISA y, lo que es m\u00e1s importante, la purificaci\u00f3n de [&hellip;]<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/pages\/11193"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/comments?post=11193"}],"version-history":[{"count":19,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/pages\/11193\/revisions"}],"predecessor-version":[{"id":11226,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/pages\/11193\/revisions\/11226"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=11193"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}