{"id":10834,"date":"2023-04-14T18:54:52","date_gmt":"2023-04-14T23:54:52","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?page_id=10834"},"modified":"2023-04-14T19:36:08","modified_gmt":"2023-04-15T00:36:08","slug":"ensayosdeproliferacion","status":"publish","type":"page","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/ensayosdeproliferacion\/","title":{"rendered":"Ensayos de proliferaci\u00f3n celular"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"10834\" class=\"elementor elementor-10834\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c0acf55 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c0acf55\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-54b0733\" data-id=\"54b0733\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-823f222 e-transform e-transform elementor-absolute elementor-hidden-tablet elementor-hidden-mobile elementor-widget elementor-widget-sidebar\" data-id=\"823f222\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_translateX_effect&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:80,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:0,&quot;sizes&quot;:[]},&quot;_transform_scaleX_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1.1,&quot;sizes&quot;:[]},&quot;_transform_scaleY_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_position&quot;:&quot;absolute&quot;,&quot;_transform_translateX_effect_tablet&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateX_effect_mobile&quot;:{&quot;unit&quot;:&quot;%&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_translateY_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scaleX_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scaleX_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scaleY_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scaleY_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"sidebar.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div id=\"product_search-1\" class=\"filter-widget product_search search widget_search\">    <form role=\"search\" class=\"blue-box ic-design\" method=\"get\"\r\n          id=\"product_search_form\" action=\"https:\/\/nuevo22.cidsamexico.com\/\">\r\n\t\t<input type=\"hidden\" name=\"post_type\" value=\"al_product\" \/>        <div class=\"ic-search-container\">\r\n\t\t\t            <input class=\"product-search-box\" type=\"search\" value=\"\" name=\"s\"\r\n                   placeholder=\"B\u00fasqueda de productos\"\/>\r\n            <input class=\"product-search-submit\" type=\"submit\" id=\"searchsubmit\"\r\n                   value=\"\"\/>\r\n        <\/div>\r\n    <\/form>\r\n\t<\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a93b9d1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"a93b9d1\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3a8e057\" data-id=\"3a8e057\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a884247 elementor-absolute elementor-invisible elementor-widget elementor-widget-heading\" data-id=\"a884247\" data-element_type=\"widget\" data-settings=\"{&quot;_position&quot;:&quot;absolute&quot;,&quot;_animation&quot;:&quot;fadeInLeft&quot;}\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h2 class=\"elementor-heading-title elementor-size-default\">Ensayos de proliferaci\u00f3n celular<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e1b8db7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e1b8db7\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-0da97e4\" data-id=\"0da97e4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-eb9cf53 elementor-widget-divider--view-line_icon elementor-view-default elementor-widget-divider--element-align-center elementor-widget elementor-widget-divider\" data-id=\"eb9cf53\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-divider{--divider-border-style:none;--divider-border-width:1px;--divider-color:#2c2c2c;--divider-icon-size:20px;--divider-element-spacing:10px;--divider-pattern-height:24px;--divider-pattern-size:20px;--divider-pattern-url:none;--divider-pattern-repeat:repeat-x}.elementor-widget-divider .elementor-divider{display:-webkit-box;display:-ms-flexbox;display:flex}.elementor-widget-divider .elementor-divider__text{font-size:15px;line-height:1;max-width:95%}.elementor-widget-divider .elementor-divider__element{margin:0 var(--divider-element-spacing);-ms-flex-negative:0;flex-shrink:0}.elementor-widget-divider .elementor-icon{font-size:var(--divider-icon-size)}.elementor-widget-divider .elementor-divider-separator{display:-webkit-box;display:-ms-flexbox;display:flex;margin:0;direction:ltr}.elementor-widget-divider--view-line_icon .elementor-divider-separator,.elementor-widget-divider--view-line_text 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.elementor-divider-separator:after,.elementor-widget-divider--separator-type-pattern:not(.elementor-widget-divider--view-line) .elementor-divider-separator:before,.elementor-widget-divider--separator-type-pattern:not([class*=elementor-widget-divider--view]) .elementor-divider-separator{width:100%;min-height:var(--divider-pattern-height);-webkit-mask-size:var(--divider-pattern-size) 100%;mask-size:var(--divider-pattern-size) 100%;-webkit-mask-repeat:var(--divider-pattern-repeat);mask-repeat:var(--divider-pattern-repeat);background-color:var(--divider-color);-webkit-mask-image:var(--divider-pattern-url);mask-image:var(--divider-pattern-url)}.elementor-widget-divider--no-spacing{--divider-pattern-size:auto}.elementor-widget-divider--bg-round{--divider-pattern-repeat:round}.rtl .elementor-widget-divider .elementor-divider__text{direction:rtl}.e-container>.elementor-widget-divider{width:var(--container-widget-width,100%);--flex-grow:var(--container-widget-flex-grow,0)}<\/style>\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t\t<div class=\"elementor-icon elementor-divider__element\">\n\t\t\t\t\t<i aria-hidden=\"true\" class=\"fas fa-th\"><\/i><\/div>\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9dd70a6 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9dd70a6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-0294b38\" data-id=\"0294b38\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-b50fbea elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b50fbea\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-d7e412f\" data-id=\"d7e412f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fb981b3 elementor-widget elementor-widget-text-editor\" data-id=\"fb981b3\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p>Cell proliferation assays are valuable tools with a wide range of applications in cell biology and drug-discovery research. They are commonly used to assess normal cell heath and are essential for evaluating the antiproliferative potency and compound toxicity of novel chemotherapeutic agents. AAT Bioquest offers several strategies for assessing cell proliferation based on tracking generations of cell division or monitoring critical parameters of cell health, including metabolic activity, DNA synthesis, or ATP concentration. Select from a broad range of traditional colorimetric reagents for determining cell proliferation, in particular tetrazolium salts, and several highly sensitive fluorescent-based assays for\u00a0<a href=\"https:\/\/www.aatbio.com\/catalog\/flow-cytometry-reagents\" target=\"_blank\" rel=\"noopener\">flow cytometry<\/a>, imaging, and microplate applications.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<div class=\"elementor-element elementor-element-d48d257 elementor-widget elementor-widget-heading\" data-id=\"d48d257\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Selecting the Right Cell Proliferation Assay<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ff00b7d elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"ff00b7d\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-d4c6ae9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d4c6ae9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-867e44a\" data-id=\"867e44a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d653b30 elementor-widget elementor-widget-text-editor\" data-id=\"d653b30\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>The most accurate method for assaying cell proliferation is to measure newly synthesized DNA via BrdU (5-bromo-2&#8242;-deoxyuridine) incorporation during DNA replication. Although highly accurate and reproducible, this method can be rather tedious as subsequent detection with fluorescent anti-BrdU antibodies is necessary to measure proliferation. Alternatively, cell proliferation can be assessed more rapidly by measuring the rate of metabolic activity with WST, resazurin, or cellular esterase cleaved dyes or tracking generations of cell division with CytoTell\u2122 dyes or CFSE. Ultimately, selecting the cell proliferation assay best suited for your purpose depends mainly upon cell type, research protocol, and the action mechanism you wish to study. The following table provides an overview of the types of cell proliferation assays available.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4b81f18 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4b81f18\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-58d16ac\" data-id=\"58d16ac\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-953ae93 elementor-widget elementor-widget-text-editor\" data-id=\"953ae93\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Tabla 1.\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9874cfa e-transform elementor-widget elementor-widget-image\" data-id=\"9874cfa\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1.3,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"211\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-300x79.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-768x203.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1536x405.png 1536w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-2048x540.png 2048w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-600x158.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-280x74.png 280w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-57046c3 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"57046c3\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-1b208ed\" data-id=\"1b208ed\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-8736ed9 elementor-widget elementor-widget-spacer\" data-id=\"8736ed9\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-column .elementor-spacer-inner{height:var(--spacer-size)}.e-container{--container-widget-width:100%}.e-container>.elementor-widget-spacer{width:var(--container-widget-width,var(--spacer-size));-ms-flex-item-align:stretch;align-self:stretch;-ms-flex-negative:0;flex-shrink:0}.e-container>.elementor-widget-spacer>.elementor-widget-container,.e-container>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer{height:100%}.e-container>.elementor-widget-spacer>.elementor-widget-container>.elementor-spacer>.elementor-spacer-inner{height:var(--container-widget-height,var(--spacer-size))}<\/style>\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2d5d1b6 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2d5d1b6\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-9952867\" data-id=\"9952867\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-0fb5c77 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0fb5c77\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-cd0a28b\" data-id=\"cd0a28b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-df13c23 elementor-widget elementor-widget-heading\" data-id=\"df13c23\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">DNA Synthesis Cell Proliferation Assays<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-344c984 elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"344c984\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-9dcdbee elementor-widget elementor-widget-text-editor\" data-id=\"9dcdbee\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Measuring newly synthesized DNA\u00a0<em>in situ<\/em>\u00a0is the most reliable and precise method for identifying proliferating cells. One way to accomplish this involves incorporating the thymidine analog, BrdU (<a href=\"https:\/\/www.aatbio.com\/products\/brdu-5-bromo-2-deoxyuridine-cas-59-14-3?unit=17030\" target=\"_blank\" rel=\"noopener\">17030<\/a>), into newly replicated DNA during the S phase of the cell cycle. Proliferating BrdU-labeled cells can then be measured using fluorescently labeled anti-BrdU antibodies. BrdU staining is suitable for various applications, including\u00a0<a href=\"https:\/\/www.aatbio.com\/catalog\/immunohistochemistry-ihc\" target=\"_blank\" rel=\"noopener\">IHC<\/a>, ICC, and\u00a0<a href=\"https:\/\/www.aatbio.com\/catalog\/enzyme-linked-immunosorbent-assay-elisa-direct-indirect-and-sandwich-assays\" target=\"_blank\" rel=\"noopener\">ELISA<\/a>, and is amenable to staining with other biomarkers of interest in flow cytometry. We offer fluorescent conjugates of the mouse monoclonal anti-BrdU antibody clone Bu20a labeled with PE (<a href=\"https:\/\/www.aatbio.com\/products\/pe-mouse-anti-brdu-antibody-bu20a-monoclonal?unit=V103305\" target=\"_blank\" rel=\"noopener\">V103305<\/a>) and unlabeled mouse monoclonal anti-BrdU antibody clone Bu20a (<a href=\"https:\/\/www.aatbio.com\/products\/purified-mouse-anti-brdu-antibody-bu20a-monoclonal?unit=V103300\" target=\"_blank\" rel=\"noopener\">V103300<\/a>) and clone MoBu-1 (<a href=\"https:\/\/www.aatbio.com\/products\/purified-mouse-anti-brdu-antibody-mobu-1-monoclonal?unit=V103295\" target=\"_blank\" rel=\"noopener\">V103295<\/a>) that can be labeled with our brightest and most photostable\u00a0<a href=\"https:\/\/www.aatbio.com\/catalog\/ifluor-dyes-and-kits\" target=\"_blank\" rel=\"noopener\">iFluor\u2122 dyes<\/a>.<br \/><br \/><\/p><h6 id=\"toc_0_1\">Click-Chemistry Driven Bucculite\u2122 XdU Cell Proliferation Assays<\/h6><p>Unlike BrdU assays, Bucculite\u2122 XdU Cell Proliferation Assays do not rely on antibodies to quantify nascent DNA, nor do they require DNA denaturation needed to facilitate antibody detection of BrdU-labeled nucleosides. Rather, Bucculite\u2122 XdU Cell Proliferation Assays use a proprietary mixture of alkyne-containing thymidine analogs, XdU, that is incorporated into newly synthesized DNA and subsequently detected via a copper-catalyzed azide-alkyne cycloaddition (i.e., click reaction, Figure 1).<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cd65bec elementor-position-top elementor-vertical-align-top elementor-widget elementor-widget-image-box\" data-id=\"cd65bec\" data-element_type=\"widget\" data-widget_type=\"image-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-image-box .elementor-image-box-content{width:100%}@media (min-width:768px){.elementor-widget-image-box.elementor-position-left .elementor-image-box-wrapper,.elementor-widget-image-box.elementor-position-right .elementor-image-box-wrapper{display:-webkit-box;display:-ms-flexbox;display:flex}.elementor-widget-image-box.elementor-position-right .elementor-image-box-wrapper{text-align:right;-webkit-box-orient:horizontal;-webkit-box-direction:reverse;-ms-flex-direction:row-reverse;flex-direction:row-reverse}.elementor-widget-image-box.elementor-position-left .elementor-image-box-wrapper{text-align:left;-webkit-box-orient:horizontal;-webkit-box-direction:normal;-ms-flex-direction:row;flex-direction:row}.elementor-widget-image-box.elementor-position-top .elementor-image-box-img{margin:auto}.elementor-widget-image-box.elementor-vertical-align-top .elementor-image-box-wrapper{-webkit-box-align:start;-ms-flex-align:start;align-items:flex-start}.elementor-widget-image-box.elementor-vertical-align-middle .elementor-image-box-wrapper{-webkit-box-align:center;-ms-flex-align:center;align-items:center}.elementor-widget-image-box.elementor-vertical-align-bottom .elementor-image-box-wrapper{-webkit-box-align:end;-ms-flex-align:end;align-items:flex-end}}@media (max-width:767px){.elementor-widget-image-box .elementor-image-box-img{margin-left:auto!important;margin-right:auto!important;margin-bottom:15px}}.elementor-widget-image-box .elementor-image-box-img{display:inline-block}.elementor-widget-image-box .elementor-image-box-title a{color:inherit}.elementor-widget-image-box .elementor-image-box-wrapper{text-align:center}.elementor-widget-image-box .elementor-image-box-description{margin:0}<\/style><div class=\"elementor-image-box-wrapper\"><figure class=\"elementor-image-box-img\"><img width=\"1200\" height=\"339\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/ClickChemistrySchematic2.webp\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" \/><\/figure><div class=\"elementor-image-box-content\"><p class=\"elementor-image-box-description\">Bucculite\u2122 XdU Cell Proliferation Assay Principle. Proliferating cells in the presence of XdU incorporate the compound at thymidine bases during the S phase. Fluorophore-labeled azide reacts with the incorporated XdU to allow detection by imaging or flow cytometry (figure made in BioRender).<\/p><\/div><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-323512c elementor-widget elementor-widget-spacer\" data-id=\"323512c\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-016cadf elementor-widget elementor-widget-text-editor\" data-id=\"016cadf\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Since the click reaction utilizes biorthogonal moieties to detect proliferating cells, background interference is minimal. More importantly, because of the mild reaction conditions, Bucculite\u2122 XdU Cell Proliferation Assays preserve cell morphology, antigen-binding sites, and sample integrity, affording the user an opportunity for multiplexing analysis with cell cycle dyes, antibodies against cell surface markers, and fluorescent proteins. After XdU incorporation, the click reaction and subsequent wash steps can be completed in less than 60 minutes, and nascent DNA can be quantified using standard imaging systems or a flow-cytometer. Also available are environmentally safe copper-free Bucculite\u2122 FdU Cell Proliferation Assays in green, red, and deep red fluorescence. These kits utilize our patent Buccutite\u2122 labeling chemistry to facilitate the detection of newly synthesized DNA in actively proliferating cells.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-765c528 elementor-position-top elementor-vertical-align-top elementor-widget elementor-widget-image-box\" data-id=\"765c528\" data-element_type=\"widget\" data-widget_type=\"image-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"elementor-image-box-wrapper\"><figure class=\"elementor-image-box-img\"><img width=\"895\" height=\"473\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Picture4.webp\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" \/><\/figure><div class=\"elementor-image-box-content\"><p class=\"elementor-image-box-description\">Bucculite\u2122 XdU Cell Proliferation Assay Principle. Proliferating cells in the presence of XdU incorporate the compound at thymidine bases during the S phase. Fluorophore-labeled azide reacts with the incorporated XdU to allow detection by imaging or flow cytometry (figure made in BioRender).<\/p><\/div><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1668d55 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1668d55\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-b0afddc\" data-id=\"b0afddc\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c963ecd elementor-widget elementor-widget-text-editor\" data-id=\"c963ecd\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Tabla 1.\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-25156cb e-transform elementor-widget elementor-widget-image\" data-id=\"25156cb\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"211\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-300x79.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-768x203.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1536x405.png 1536w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-2048x540.png 2048w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-600x158.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-280x74.png 280w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-3653c41 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"3653c41\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6af0fb5\" data-id=\"6af0fb5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-bef24fc elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"bef24fc\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-6b725d7\" data-id=\"6b725d7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a38bb6e elementor-widget elementor-widget-heading\" data-id=\"a38bb6e\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Dye Dilution Assays for Cell Proliferation<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-387245e elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"387245e\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-17cd8d1 elementor-widget elementor-widget-text-editor\" data-id=\"17cd8d1\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>CFSE and CytoTell\u2122 indicators are fluorescent tracking dyes useful for monitoring cell proliferation in living cells by flow cytometry. As cells divide, the fluorescent dye is uniformly distributed between daughter cells, resulting in the progressive halving of fluorescence intensity with each successive generation. These proliferation dyes can be used to track cell proliferation long-term both\u00a0<em>in vitro<\/em>\u00a0and\u00a0<em>in vivo<\/em>\u00a0for several generations.<br \/><br \/><\/p><h6 id=\"toc_0_2\">Proliferation Assay Using CFSE<\/h6><p>CFSE (Carboxyfluorescein diacetate, succinimidyl ester,\u00a0<a href=\"https:\/\/www.aatbio.com\/products\/cfse-5-and-6-carboxyfluorescein-diacetate-succinimidyl-ester-cas-150347-59-4?unit=22022\" target=\"_blank\" rel=\"noopener\">22022<\/a>) and ReadiUse\u2122 CFSE (<a href=\"https:\/\/www.aatbio.com\/products\/readiuse-cfse-5-and-6-carboxyfluorescein-diacetate-succinimidyl-ester-cas-150347-59-4?unit=22028\" target=\"_blank\" rel=\"noopener\">22028<\/a>) are cell-permeable green fluorescent proliferation indicators that emit a fluorescence signal at 517 nm when excited by the 488 nm argon-ion laser. Both dyes passively diffuse across uncompromised cell membranes, whereby enzyme reactions with intracellular esterases cleave acetate groups from CFSE to yield an amine-reactive carboxyfluorescein, SE that covalently binds to proteins within the cell. While CFSE remains widely used as a green fluorescent proliferation indicator excited at 498 nm, it is, however, not without caveats. CFSE is highly toxic to cells, susceptible to dye leakage, and is known to spill over into the PE and PE-Texas Red channels.<br \/><br \/><\/p><h6 id=\"toc_0_3\">CytoTell\u2122 Proliferation Indicators<\/h6><p>CytoTell\u2122 dyes are a series of cell-permeable fluorescent tracers designed to monitor cell proliferation in living cells without any of the drawbacks of CFSE. Upon passive diffusion across cell membranes, CytoTell\u2122 dyes are hydrolyzed by intracellular esterases to yield highly fluorescent amine-reactive or thiol-reactive dyes that irreversibly bind to amine or thiol groups on intracellular proteins. Cytotoxicity is minimal, and the dyes are well-retained within cells through several generations of division. Visualize up to 9 generations using CytoTell\u2122 UltraGreen (<a href=\"https:\/\/www.aatbio.com\/products\/cytotell-ultragreen?unit=22240\" target=\"_blank\" rel=\"noopener\">22240<\/a>). CytoTell\u2122 dyes are available in 7 different fluorescence emission colors offering greater flexibility in multicolor analysis. They may be fixed and permeabilized to analyze intracellular targets using standard formaldehyde-containing fixatives and saponin-based permeabilization buffers.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-e3df1f9 elementor-position-top elementor-vertical-align-top elementor-widget elementor-widget-image-box\" data-id=\"e3df1f9\" data-element_type=\"widget\" data-widget_type=\"image-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"elementor-image-box-wrapper\"><figure class=\"elementor-image-box-img\"><img width=\"1000\" height=\"569\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/791a14899086d76768f8.webp\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" \/><\/figure><div class=\"elementor-image-box-content\"><p class=\"elementor-image-box-description\">Cell proliferation assay using CytoTell\u2122 Green and CFSE. Jurkat cells (~2x106 cells\/mL) were stained with CytoTell\u2122 Green or CFSE on Day 0. Cells were then passed serially at a 1:1 ratio on the day specified. Fluorescence intensity was measured with ACEA NovoCyte 3000 flow cytometer in the FITC channel on the day of passage. Different color peaks represent successive generations.\n<\/p><\/div><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-de40545 elementor-widget elementor-widget-spacer\" data-id=\"de40545\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-e1fb264 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"e1fb264\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-ef7038e\" data-id=\"ef7038e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ed8f9bc elementor-widget elementor-widget-text-editor\" data-id=\"ed8f9bc\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Tabla 1.\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-c91b819 e-transform elementor-widget elementor-widget-image\" data-id=\"c91b819\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"211\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-300x79.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-768x203.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1536x405.png 1536w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-2048x540.png 2048w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-600x158.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-280x74.png 280w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b5e26ec elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b5e26ec\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-852e161\" data-id=\"852e161\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-120eae4 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"120eae4\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-fd7992d\" data-id=\"fd7992d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-172953f elementor-widget elementor-widget-heading\" data-id=\"172953f\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Metabolic Assays for Cell Proliferation<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1e19a5e elementor-widget-divider--view-line elementor-widget elementor-widget-divider\" data-id=\"1e19a5e\" data-element_type=\"widget\" data-widget_type=\"divider.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-divider\">\n\t\t\t<span class=\"elementor-divider-separator\">\n\t\t\t\t\t\t<\/span>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6c486ec elementor-widget elementor-widget-text-editor\" data-id=\"6c486ec\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Metabolic indicators, such as tetrazolium salts and resazurin, measure the cellular reduction potential in metabolically active cells to evaluate proliferation. Following reduction, these substrates yield bright colorimetric products that can be measured using a spectrophotometer or microplate reader in low- or high-throughput configurations. Reduced resazurin can also be measured using a fluorescence microplate reader.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-712db60 elementor-widget elementor-widget-spacer\" data-id=\"712db60\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-30b8ac9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"30b8ac9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-6d4ba8d\" data-id=\"6d4ba8d\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-59b4e60 elementor-widget elementor-widget-text-editor\" data-id=\"59b4e60\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h6 id=\"toc_0_4\">Proliferation Assay Using Tetrazolium Salts<\/h6><p>Metabolic indicators, such as tetrazolium salts and resazurin, measure the cellular reduction potential in metabolically active cells to evaluate proliferation. Following reduction, these substrates yield bright colorimetric products that can be measured using a spectrophotometer or microplate reader in low- or high-throughput configurations. Reduced resazurin can also be measured using a fluorescence microplate reader.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-8496236\" data-id=\"8496236\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bd6a22c elementor-position-top elementor-vertical-align-top elementor-widget elementor-widget-image-box\" data-id=\"bd6a22c\" data-element_type=\"widget\" data-widget_type=\"image-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"elementor-image-box-wrapper\"><figure class=\"elementor-image-box-img\"><img width=\"1222\" height=\"883\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/22768_3.svg\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" \/><\/figure><div class=\"elementor-image-box-content\"><p class=\"elementor-image-box-description\">Cell proliferation was determined using Cell Meter\u2122 Colorimetric MTT Cell Proliferation Kit. HeLa cells at 0 to 40,000 cells\/well\/100 \u00b5L were added to a clear bottom 96-well plate overnight. The absorbance was measured at 560 nm using a SpectraMax reader (Molecular Devices). 500 cells\/well was detected compare to ~5,000 cells\/well with Sigma's MTT assay kit.<\/p><\/div><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-cb742d1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"cb742d1\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-7933eb7\" data-id=\"7933eb7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bc29a51 elementor-widget elementor-widget-spacer\" data-id=\"bc29a51\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-97b830f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"97b830f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-9fe87c1\" data-id=\"9fe87c1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-2407ce5 elementor-widget elementor-widget-text-editor\" data-id=\"2407ce5\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p id=\"toc_0_4\">Among the other tetrazolium salts, the water-soluble WST-8 offers the highest sensitivity. Reduction of WST-8 by cellular dehydrogenases yields water-soluble orange formazan crystals that do not require solubilization prior to quantification. The concentration of formazan product can be determined by measuring absorbance at 460 nm and is proportional to the number of living cells. The Cell Meter\u2122 Colorimetric WST-8 Cell Quantification Kit provides a sensitive assay with exceptional linearity of up to ~10<sup>6<\/sup>\u00a0cells per well. No pre-mixing of components is required, as the WST-8 substrate is conveniently provided in a solution that can be directly added to the cells. Each kit provides sufficient reagents for either ~1000 (<a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-colorimetric-wst-8-cell-quantification-kit?unit=22770\" target=\"_blank\" rel=\"noopener\">22770<\/a>) or ~5000 tests (<a href=\"https:\/\/www.aatbio.com\/products\/cell-meter-colorimetric-wst-8-cell-quantification-kit?unit=22771\" target=\"_blank\" rel=\"noopener\">22771<\/a>) using a standard 96-well microplate. WST-8 is also available as a stand-alone reagent in 25 mg (<a href=\"https:\/\/www.aatbio.com\/products\/readiuse-wst-8-50-mm-aqueous-solution?unit=15705\" target=\"_blank\" rel=\"noopener\">15705<\/a>), 100 mg (<a href=\"https:\/\/www.aatbio.com\/products\/readiuse-wst-8-50-mm-aqueous-solution?unit=15706\" target=\"_blank\" rel=\"noopener\">15706<\/a>), and 1 g (<a href=\"https:\/\/www.aatbio.com\/products\/readiuse-wst-8-50-mm-aqueous-solution?unit=15707\" target=\"_blank\" rel=\"noopener\">15707<\/a>) unit sizes supplied as 50 mM aqueous solutions.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-52ec1bd\" data-id=\"52ec1bd\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a88665b elementor-position-top elementor-vertical-align-top elementor-widget elementor-widget-image-box\" data-id=\"a88665b\" data-element_type=\"widget\" data-widget_type=\"image-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"elementor-image-box-wrapper\"><figure class=\"elementor-image-box-img\"><img width=\"1222\" height=\"883\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/22768_3.svg\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" \/><\/figure><div class=\"elementor-image-box-content\"><p class=\"elementor-image-box-description\">Cell proliferation was determined using Cell Meter\u2122 Colorimetric MTT Cell Proliferation Kit. HeLa cells at 0 to 40,000 cells\/well\/100 \u00b5L were added to a clear bottom 96-well plate overnight. The absorbance was measured at 560 nm using a SpectraMax reader (Molecular Devices). 500 cells\/well was detected compare to ~5,000 cells\/well with Sigma's MTT assay kit.<\/p><\/div><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-5146a10 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"5146a10\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-878f6fd\" data-id=\"878f6fd\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-c25b262 elementor-widget elementor-widget-text-editor\" data-id=\"c25b262\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Tabla 1.\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f1e1cb6 e-transform elementor-widget elementor-widget-image\" data-id=\"f1e1cb6\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1.1,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"211\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-300x79.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-768x203.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1536x405.png 1536w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-2048x540.png 2048w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-600x158.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-280x74.png 280w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fb7a4a8 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fb7a4a8\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-7aaafef\" data-id=\"7aaafef\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-inner-section elementor-element elementor-element-8894bd3 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8894bd3\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-inner-column elementor-element elementor-element-0487001\" data-id=\"0487001\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4ac9cdb elementor-widget elementor-widget-heading\" data-id=\"4ac9cdb\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Proliferation Assay Using Resazurin<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-dd89aea elementor-widget elementor-widget-text-editor\" data-id=\"dd89aea\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Like the aforementioned tetrazolium salts, the oxidation-reduction indicator resazurin (<a href=\"https:\/\/www.aatbio.com\/products\/resazurin-sodium-salt-cas-62758-13-8?unit=15700\" target=\"_blank\" rel=\"noopener\">15700<\/a>) can be useful for quantifying cell proliferation. Following reduction, the blue non-fluorescent resazurin yields a pink-colored and highly fluorescent resorufin product that is compatible with both fluorescence and absorbance microplate readers. The fluorescent or colorimetric signal is proportional to the number of living cells in the sample. Resazurin has been reported to be successfully used in a wide range of cell types, including bacteria, yeast, fungi, protozoa, and cultured mammalian cells.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-a075bbd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"a075bbd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f1983c5\" data-id=\"f1983c5\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-058caea elementor-widget elementor-widget-text-editor\" data-id=\"058caea\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Tabla 5.\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-626bfb2 e-transform elementor-widget elementor-widget-image\" data-id=\"626bfb2\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"56\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.22.36-1024x72.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.22.36-1024x72.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.22.36-300x21.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.22.36-768x54.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.22.36-1536x108.png 1536w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.22.36-2048x144.png 2048w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.22.36-600x42.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.22.36-280x20.png 280w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d5026eb elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d5026eb\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-e44bedf\" data-id=\"e44bedf\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-44c4441 elementor-widget elementor-widget-text-editor\" data-id=\"44c4441\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h4 id=\"ATP_Assays\">ATP Assays for Cell Proliferation<\/h4><p>ATP, which is present in all metabolically active cells and virtually non-existent in dead cells, is a suitable biomarker for identifying viable cells. Assays for quantifying ATP have been used to determine cell proliferation and cytotoxicity in both bacterial and mammalian cells and for detecting low-level bacterial contamination in samples such as water, soil, and blood. While several detection methods can be used, researchers commonly choose luciferin-luciferase bioluminescence-based ATP assays to measure cell proliferation due to its higher sensitivity. The PhosphoWorks\u2122 Luminometric ATP Assay Kits provide a simple and homogeneous method for measuring ATP using firefly luciferase and its substrate luciferin. In the presence of Mg<sup>2+<\/sup>, firefly luciferase catalyzes the reaction of luciferin, ATP, and O<sub>2<\/sub>\u00a0to produce light at ~560 nm that can be detected using a luminometer. The concentration of ATP present is proportional to the number of viable cells in the sample. Also available is a PhosphoWorks\u2122 Colorimetric ATP Assay Kit (<a href=\"https:\/\/www.aatbio.com\/products\/phosphoworks-colorimetric-atp-assay-kit?unit=21617\" target=\"_blank\" rel=\"noopener\">21617<\/a>) and a PhosphoWorks\u2122 Fluorimetric ATP Assay Kit (<a href=\"https:\/\/www.aatbio.com\/products\/phosphoworks-fluorimetric-atp-assay-kit?unit=21620\" target=\"_blank\" rel=\"noopener\">21620<\/a>) that can detect ~3 \u00b5M or ~0.4 \u00b5M of ATP in a 100 \u00b5L reaction volume, respectively.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-a6fc265\" data-id=\"a6fc265\" data-element_type=\"column\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b0e7fb4 elementor-position-top elementor-vertical-align-top elementor-widget elementor-widget-image-box\" data-id=\"b0e7fb4\" data-element_type=\"widget\" data-widget_type=\"image-box.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<div class=\"elementor-image-box-wrapper\"><figure class=\"elementor-image-box-img\"><img width=\"1222\" height=\"883\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/22768_3.svg\" class=\"attachment-full size-full\" alt=\"\" loading=\"lazy\" \/><\/figure><div class=\"elementor-image-box-content\"><p class=\"elementor-image-box-description\">Cell proliferation was determined using Cell Meter\u2122 Colorimetric MTT Cell Proliferation Kit. HeLa cells at 0 to 40,000 cells\/well\/100 \u00b5L were added to a clear bottom 96-well plate overnight. The absorbance was measured at 560 nm using a SpectraMax reader (Molecular Devices). 500 cells\/well was detected compare to ~5,000 cells\/well with Sigma's MTT assay kit.<\/p><\/div><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fbd5d39 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fbd5d39\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-a6e2b49\" data-id=\"a6e2b49\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f529d15 elementor-widget elementor-widget-text-editor\" data-id=\"f529d15\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Tabla 1.\u00a0<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-1057528 e-transform elementor-widget elementor-widget-image\" data-id=\"1057528\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"211\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1024x270.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-300x79.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-768x203.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-1536x405.png 1536w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-2048x540.png 2048w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-600x158.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-14-at-18.03.44-280x74.png 280w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-df99ff6 elementor-widget elementor-widget-spacer\" data-id=\"df99ff6\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-8d5ce23 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8d5ce23\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-9505c7e\" data-id=\"9505c7e\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1d7e333 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1d7e333\" data-element_type=\"section\" data-settings=\"{&quot;background_background&quot;:&quot;classic&quot;}\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-8a67f89\" data-id=\"8a67f89\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-63339de\" data-id=\"63339de\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cd38364 elementor-widget elementor-widget-spacer\" data-id=\"cd38364\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7743e5b elementor-widget elementor-widget-heading\" data-id=\"7743e5b\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">P\u00e1ginas<\/h5>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-931fba5 elementor-mobile-align-center elementor-icon-list--layout-traditional elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list\" data-id=\"931fba5\" data-element_type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<link rel=\"stylesheet\" href=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/plugins\/elementor\/assets\/css\/widget-icon-list.min.css\">\t\t<ul class=\"elementor-icon-list-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/nuevo22.cidsamexico.com\/\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Home<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a 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class=\"elementor-icon-list-text\">Terminos y Condiciones<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/nuevo22.cidsamexico.com\/index.php\/uneteequipo\/\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">\u00danete a nuestro equipo<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3301cda elementor-widget elementor-widget-spacer\" data-id=\"3301cda\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-1773b56\" data-id=\"1773b56\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f75151a elementor-widget elementor-widget-spacer\" data-id=\"f75151a\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-edf3ee1 elementor-widget elementor-widget-heading\" data-id=\"edf3ee1\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Laboratorios y oficinas<\/h5>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ba6d3b4 elementor-list-item-link-inline elementor-mobile-align-center elementor-icon-list--layout-traditional elementor-widget elementor-widget-icon-list\" data-id=\"ba6d3b4\" data-element_type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<ul class=\"elementor-icon-list-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Oficinas y Almac\u00e9n en CDMX<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Laboratorios en Zacatecas<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Matriz y Laboratorios en Queretaro <\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-71aa844 elementor-widget elementor-widget-spacer\" data-id=\"71aa844\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-08c9058\" data-id=\"08c9058\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-6f16262 elementor-widget elementor-widget-spacer\" data-id=\"6f16262\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-eda6471 elementor-widget elementor-widget-heading\" data-id=\"eda6471\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Contacto<\/h5>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bdd6327 elementor-mobile-align-center elementor-icon-list--layout-traditional elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list\" data-id=\"bdd6327\" data-element_type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<ul class=\"elementor-icon-list-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t<i aria-hidden=\"true\" class=\"fab fa-whatsapp\"><\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">55-3621-4100<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t<i aria-hidden=\"true\" class=\"fas fa-phone-alt\"><\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">55-8961-0759 <\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t<i aria-hidden=\"true\" class=\"fas fa-phone-alt\"><\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Zacatecas 492-9219474<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t<i aria-hidden=\"true\" class=\"far fa-envelope-open\"><\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">ventas@cidsamexico.com<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\"><\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Soporte T\u00e9cnico<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t<i aria-hidden=\"true\" class=\"far fa-envelope-open\"><\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">stecnico@cidsamexico.com<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-d26b810\" data-id=\"d26b810\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-81fcb9e elementor-widget elementor-widget-image\" data-id=\"81fcb9e\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"239\" height=\"160\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/cidsatrans-239x160.png\" class=\"attachment-classic-list-listing size-classic-list-listing\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/cidsatrans-239x160.png 239w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/cidsatrans-300x201.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/cidsatrans.png 512w\" sizes=\"(max-width: 239px) 100vw, 239px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-57d8b67 elementor-widget elementor-widget-spacer\" data-id=\"57d8b67\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-26d3a5e elementor-widget__width-initial elementor-absolute elementor-widget elementor-widget-text-editor\" data-id=\"26d3a5e\" data-element_type=\"widget\" data-settings=\"{&quot;_position&quot;:&quot;absolute&quot;}\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Somos una empresa y laboratorio con amplia experiencia. Con\u00f3cenos.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-7017b40 elementor-grid-3 elementor-shape-rounded e-grid-align-center elementor-invisible elementor-widget elementor-widget-social-icons\" data-id=\"7017b40\" data-element_type=\"widget\" data-settings=\"{&quot;_animation&quot;:&quot;zoomIn&quot;}\" data-widget_type=\"social-icons.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-social-icons.elementor-grid-0 .elementor-widget-container,.elementor-widget-social-icons.elementor-grid-mobile-0 .elementor-widget-container,.elementor-widget-social-icons.elementor-grid-tablet-0 .elementor-widget-container{line-height:1;font-size:0}.elementor-widget-social-icons:not(.elementor-grid-0):not(.elementor-grid-tablet-0):not(.elementor-grid-mobile-0) .elementor-grid{display:inline-grid}.elementor-widget-social-icons .elementor-grid{grid-column-gap:var(--grid-column-gap,5px);grid-row-gap:var(--grid-row-gap,5px);grid-template-columns:var(--grid-template-columns);-webkit-box-pack:var(--justify-content,center);-ms-flex-pack:var(--justify-content,center);justify-content:var(--justify-content,center);justify-items:var(--justify-content,center)}.elementor-icon.elementor-social-icon{font-size:var(--icon-size,25px);line-height:var(--icon-size,25px);width:calc(var(--icon-size, 25px) + (2 * var(--icon-padding, .5em)));height:calc(var(--icon-size, 25px) + (2 * var(--icon-padding, .5em)))}.elementor-social-icon{--e-social-icon-icon-color:#fff;display:-webkit-inline-box;display:-ms-inline-flexbox;display:inline-flex;background-color:#818a91;-webkit-box-align:center;-ms-flex-align:center;align-items:center;-webkit-box-pack:center;-ms-flex-pack:center;justify-content:center;text-align:center;cursor:pointer}.elementor-social-icon i{color:var(--e-social-icon-icon-color)}.elementor-social-icon svg{fill:var(--e-social-icon-icon-color)}.elementor-social-icon:last-child{margin:0}.elementor-social-icon:hover{opacity:.9;color:#fff}.elementor-social-icon-android{background-color:#a4c639}.elementor-social-icon-apple{background-color:#999}.elementor-social-icon-behance{background-color:#1769ff}.elementor-social-icon-bitbucket{background-color:#205081}.elementor-social-icon-codepen{background-color:#000}.elementor-social-icon-delicious{background-color:#39f}.elementor-social-icon-deviantart{background-color:#05cc47}.elementor-social-icon-digg{background-color:#005be2}.elementor-social-icon-dribbble{background-color:#ea4c89}.elementor-social-icon-elementor{background-color:#d30c5c}.elementor-social-icon-envelope{background-color:#ea4335}.elementor-social-icon-facebook,.elementor-social-icon-facebook-f{background-color:#3b5998}.elementor-social-icon-flickr{background-color:#0063dc}.elementor-social-icon-foursquare{background-color:#2d5be3}.elementor-social-icon-free-code-camp,.elementor-social-icon-freecodecamp{background-color:#006400}.elementor-social-icon-github{background-color:#333}.elementor-social-icon-gitlab{background-color:#e24329}.elementor-social-icon-globe{background-color:#818a91}.elementor-social-icon-google-plus,.elementor-social-icon-google-plus-g{background-color:#dd4b39}.elementor-social-icon-houzz{background-color:#7ac142}.elementor-social-icon-instagram{background-color:#262626}.elementor-social-icon-jsfiddle{background-color:#487aa2}.elementor-social-icon-link{background-color:#818a91}.elementor-social-icon-linkedin,.elementor-social-icon-linkedin-in{background-color:#0077b5}.elementor-social-icon-medium{background-color:#00ab6b}.elementor-social-icon-meetup{background-color:#ec1c40}.elementor-social-icon-mixcloud{background-color:#273a4b}.elementor-social-icon-odnoklassniki{background-color:#f4731c}.elementor-social-icon-pinterest{background-color:#bd081c}.elementor-social-icon-product-hunt{background-color:#da552f}.elementor-social-icon-reddit{background-color:#ff4500}.elementor-social-icon-rss{background-color:#f26522}.elementor-social-icon-shopping-cart{background-color:#4caf50}.elementor-social-icon-skype{background-color:#00aff0}.elementor-social-icon-slideshare{background-color:#0077b5}.elementor-social-icon-snapchat{background-color:#fffc00}.elementor-social-icon-soundcloud{background-color:#f80}.elementor-social-icon-spotify{background-color:#2ebd59}.elementor-social-icon-stack-overflow{background-color:#fe7a15}.elementor-social-icon-steam{background-color:#00adee}.elementor-social-icon-stumbleupon{background-color:#eb4924}.elementor-social-icon-telegram{background-color:#2ca5e0}.elementor-social-icon-thumb-tack{background-color:#1aa1d8}.elementor-social-icon-tripadvisor{background-color:#589442}.elementor-social-icon-tumblr{background-color:#35465c}.elementor-social-icon-twitch{background-color:#6441a5}.elementor-social-icon-twitter{background-color:#1da1f2}.elementor-social-icon-viber{background-color:#665cac}.elementor-social-icon-vimeo{background-color:#1ab7ea}.elementor-social-icon-vk{background-color:#45668e}.elementor-social-icon-weibo{background-color:#dd2430}.elementor-social-icon-weixin{background-color:#31a918}.elementor-social-icon-whatsapp{background-color:#25d366}.elementor-social-icon-wordpress{background-color:#21759b}.elementor-social-icon-xing{background-color:#026466}.elementor-social-icon-yelp{background-color:#af0606}.elementor-social-icon-youtube{background-color:#cd201f}.elementor-social-icon-500px{background-color:#0099e5}.elementor-shape-rounded .elementor-icon.elementor-social-icon{border-radius:10%}.elementor-shape-circle .elementor-icon.elementor-social-icon{border-radius:50%}<\/style>\t\t<div class=\"elementor-social-icons-wrapper elementor-grid\">\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-facebook elementor-repeater-item-da64bc7\" href=\"http:\/\/www.facebook.com\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Facebook<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-facebook\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-twitter elementor-repeater-item-0b93d4e\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Twitter<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-twitter\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-linkedin elementor-repeater-item-50b061f\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Linkedin<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-linkedin\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-d20bf50 elementor-widget elementor-widget-spacer\" data-id=\"d20bf50\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-0f904ed elementor-section-boxed elementor-section-height-default elementor-section-height-default\" 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All rights reserved<\/span><\/p><p style=\"text-align: center;\"><span style=\"font-size: 9px; text-align: center;\">Dise\u00f1ado por Suguza Publicidad<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Ensayos de proliferaci\u00f3n celular Cell proliferation assays are valuable tools with a wide range of applications in cell biology and drug-discovery research. They are commonly used to assess normal cell heath and are essential for evaluating the antiproliferative potency and compound toxicity of novel chemotherapeutic agents. 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