{"id":10161,"date":"2023-04-03T17:33:39","date_gmt":"2023-04-03T22:33:39","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?page_id=10161"},"modified":"2023-04-03T18:09:42","modified_gmt":"2023-04-03T23:09:42","slug":"nanodiscos","status":"publish","type":"page","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/nanodiscos\/","title":{"rendered":"Nanodiscos"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-page\" data-elementor-id=\"10161\" class=\"elementor elementor-10161\">\n\t\t\t\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-f9a6edb elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"f9a6edb\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f8a95fd\" data-id=\"f8a95fd\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-67bc06c elementor-widget elementor-widget-heading\" data-id=\"67bc06c\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-heading-title{padding:0;margin:0;line-height:1}.elementor-widget-heading .elementor-heading-title[class*=elementor-size-]>a{color:inherit;font-size:inherit;line-height:inherit}.elementor-widget-heading .elementor-heading-title.elementor-size-small{font-size:15px}.elementor-widget-heading .elementor-heading-title.elementor-size-medium{font-size:19px}.elementor-widget-heading .elementor-heading-title.elementor-size-large{font-size:29px}.elementor-widget-heading .elementor-heading-title.elementor-size-xl{font-size:39px}.elementor-widget-heading .elementor-heading-title.elementor-size-xxl{font-size:59px}<\/style><h2 class=\"elementor-heading-title elementor-size-default\">What Are Nanodiscs?<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-12f645e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"12f645e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-3259997\" data-id=\"3259997\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-7e226eb elementor-widget elementor-widget-text-editor\" data-id=\"7e226eb\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-text-editor.elementor-drop-cap-view-stacked .elementor-drop-cap{background-color:#818a91;color:#fff}.elementor-widget-text-editor.elementor-drop-cap-view-framed .elementor-drop-cap{color:#818a91;border:3px solid;background-color:transparent}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap{margin-top:8px}.elementor-widget-text-editor:not(.elementor-drop-cap-view-default) .elementor-drop-cap-letter{width:1em;height:1em}.elementor-widget-text-editor .elementor-drop-cap{float:left;text-align:center;line-height:1;font-size:50px}.elementor-widget-text-editor .elementor-drop-cap-letter{display:inline-block}<\/style>\t\t\t\t<p>The term nanodiscs describes a small (7-50 nm in diameter) disc-shaped structure that finds use in proteomics and biomedicine. It consists of two main components:<\/p><ol><li><strong>Phospholipids,<\/strong>\u00a0either of the artificial origin or from the cell membrane<\/li><li><strong>A\u00a0<\/strong><strong>stabilizing belt<\/strong>\u00a0that holds the phospholipids together. This is either an MSP protein or a\u00a0synthetic polymer.<\/li><\/ol>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7902073 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7902073\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-97e2997\" data-id=\"97e2997\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-f83e25a e-transform elementor-widget elementor-widget-image\" data-id=\"f83e25a\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:0.8,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-image{text-align:center}.elementor-widget-image a{display:inline-block}.elementor-widget-image a img[src$=\".svg\"]{width:48px}.elementor-widget-image img{vertical-align:middle;display:inline-block}<\/style>\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"646\" height=\"446\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/MSP_disc-mit-membran-protein_klein.jpg\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/MSP_disc-mit-membran-protein_klein.jpg 646w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/MSP_disc-mit-membran-protein_klein-300x207.jpg 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/MSP_disc-mit-membran-protein_klein-600x414.jpg 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/MSP_disc-mit-membran-protein_klein-232x160.jpg 232w\" sizes=\"(max-width: 646px) 100vw, 646px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-04fb8a8\" data-id=\"04fb8a8\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-1f00d4c elementor-widget elementor-widget-text-editor\" data-id=\"1f00d4c\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>\u00a0<\/p><p>\u00a0<\/p><p>\u00a0<\/p><p>\u00a0<\/p><p>\u00a0<\/p><p>Schematic depiction of a nanodisc stabilizing a membrane protein.<br \/><span style=\"color: #008000;\">Green:<\/span> The stabilizing belt &#8211; A MSP protein in this case<br \/><span style=\"color: #808080;\">Grey:<\/span> Phospholipids<br \/><span style=\"color: #ff6600;\">Orange:<\/span> Stabilized membrane protein<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-d01bbf6\" data-id=\"d01bbf6\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-58f478e elementor-widget elementor-widget-text-editor\" data-id=\"58f478e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6e5027d elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6e5027d\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-d2a9f6c\" data-id=\"d2a9f6c\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-4ddff3e elementor-widget elementor-widget-text-editor\" data-id=\"4ddff3e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<h3 id=\"reason\">The Purpose of Nanodiscs<\/h3><div>Their aim is to mimic the native phospholipid bilayer of cells for target molecules (often membrane proteins). Membrane proteins are the key to communication between cells. They mediate fundamental biological processes such as signal transduction, transport processes across membranes, sensing of chemical signals, and coordination of cell-cell interactions.<\/div><div>Numerous diseases in humans are linked to membrane proteins, making them important targets for drug development. So it seems surprising, that membrane proteins are encoded by up to \u223c23% of genes but represent &lt;1 % of known protein structures (1).<\/div>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-6a503fb elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"6a503fb\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-fa08700\" data-id=\"fa08700\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ef46b2f e-transform elementor-widget elementor-widget-image\" data-id=\"ef46b2f\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"450\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc_proteinfolding_hydrophil-80-1024x576.jpg\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc_proteinfolding_hydrophil-80-1024x576.jpg 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc_proteinfolding_hydrophil-80-300x169.jpg 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc_proteinfolding_hydrophil-80-768x432.jpg 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc_proteinfolding_hydrophil-80-600x338.jpg 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc_proteinfolding_hydrophil-80-280x158.jpg 280w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc_proteinfolding_hydrophil-80.jpg 1505w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-573c748 elementor-widget elementor-widget-text-editor\" data-id=\"573c748\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Figure 1: Membrane proteins have hydrophobic and hydrophilic parts. Nanodiscs make them soluble in aqueous solutions.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-8de4ea9 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"8de4ea9\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-75a44a9\" data-id=\"75a44a9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b9a17c8 elementor-widget elementor-widget-text-editor\" data-id=\"b9a17c8\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>The problem is, that membrane proteins, unlike soluble proteins, are difficult to analyze in their native environment, due to their insertion in the lipid membrane. Surfaces of membrane proteins in the hydrophobic core of the lipid bilayer are also hydrophobic, whereas surface areas in contact with the aqueous membrane environment are as hydrophilic as the surfaces of ordinary soluble proteins (2). The presence of extensive hydrophobic and hydrophilic surfaces on the same molecule is characteristic of membrane proteins. As a result, membrane proteins are not soluble in standard aqueous buffers without a solubilizing agent. For example, nanodiscs are required to solubilize them to mimic the amphipathic environment of a lipid bilayer whilst maintaining the structure of the membrane protein in a physiologically relevant state.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-9b75366 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"9b75366\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-dc12ae9\" data-id=\"dc12ae9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-9c7b7f3\" data-id=\"9c7b7f3\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-bb3f457 e-transform elementor-aspect-ratio-169 elementor-widget elementor-widget-video\" data-id=\"bb3f457\" data-element_type=\"widget\" data-settings=\"{&quot;youtube_url&quot;:&quot;https:\\\/\\\/youtu.be\\\/q276M_PRnFA&quot;,&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:0.9,&quot;sizes&quot;:[]},&quot;video_type&quot;:&quot;youtube&quot;,&quot;controls&quot;:&quot;yes&quot;,&quot;aspect_ratio&quot;:&quot;169&quot;,&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"video.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-video .elementor-widget-container{overflow:hidden;-webkit-transform:translateZ(0);transform:translateZ(0)}.elementor-widget-video .elementor-open-inline .elementor-custom-embed-image-overlay{position:absolute;top:0;left:0;width:100%;height:100%;background-size:cover;background-position:50%}.elementor-widget-video .elementor-custom-embed-image-overlay{cursor:pointer;text-align:center}.elementor-widget-video .elementor-custom-embed-image-overlay:hover .elementor-custom-embed-play i{opacity:1}.elementor-widget-video .elementor-custom-embed-image-overlay img{display:block;width:100%}.elementor-widget-video .e-hosted-video .elementor-video{-o-object-fit:cover;object-fit:cover}.e-container>.elementor-widget-video{width:var(--container-widget-width,100%)}<\/style>\t\t<div class=\"elementor-wrapper elementor-fit-aspect-ratio elementor-open-inline\">\n\t\t\t<div class=\"elementor-video\"><\/div>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-33 elementor-top-column elementor-element elementor-element-2f9e101\" data-id=\"2f9e101\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap\">\n\t\t\t\t\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-1546b58 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"1546b58\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-9e38597\" data-id=\"9e38597\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-99af9c2 elementor-widget elementor-widget-heading\" data-id=\"99af9c2\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">Two Types of Nanodisc<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d481bbd elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d481bbd\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-4072c0f\" data-id=\"4072c0f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-38c9bd5 e-transform elementor-widget elementor-widget-image\" data-id=\"38c9bd5\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"250\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc-compared-1024x320.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc-compared-1024x320.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc-compared-300x94.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc-compared-768x240.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc-compared-600x188.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc-compared-280x88.png 280w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Nanodisc-compared.png 1151w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-6caabc2 elementor-widget elementor-widget-text-editor\" data-id=\"6caabc2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Figure 2: Two types of nanodiscs exist: Synthetic nanodiscs (blue stabilizer) and MSP nanodiscs (green stabilizer).<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-0fc1c90 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"0fc1c90\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-2018a63\" data-id=\"2018a63\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d96035e elementor-widget elementor-widget-text-editor\" data-id=\"d96035e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>As mentioned before nanodiscs can be differentiated between their phospholipid composition and most importantly, their type of stabilizer. This stabilizer is the reason why nanodiscs in total are split into two main categories:\u00a0<strong>MSP nanodiscs<\/strong>\u00a0and\u00a0<strong>Synthetic nanodiscs<\/strong>.<\/p><p><br \/>The respective names originate from the type of stabilizer that is used to keep the nanodiscs together and form them in the first place. It also decides what the lipid composition of the nanodisc is made up of. MSP nanodiscs always contain an artificial lipid composition. Meaning you have full control of it. In contrast: Synthetic nanodiscs use the native cell phospholipids to create the nanodisc. A direct comparison of the\u00a0<a href=\"https:\/\/cube-biotech.com\/what-are-nanodiscs#which\">individual advantages of both nanodiscs<\/a>\u00a0can be found here.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-7525134 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"7525134\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f42baa6\" data-id=\"f42baa6\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0e8b441 elementor-widget elementor-widget-text-editor\" data-id=\"0e8b441\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>able 1: Small comparison between MSP and Synthetic nanodiscs<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-834abd1 elementor-widget elementor-widget-image\" data-id=\"834abd1\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"166\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.50.05-1024x213.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.50.05-1024x213.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.50.05-300x62.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.50.05-768x159.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.50.05-1536x319.png 1536w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.50.05-2048x425.png 2048w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.50.05-600x125.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.50.05-280x58.png 280w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-60be80a elementor-widget elementor-widget-text-editor\" data-id=\"60be80a\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>But first, let us introduce both kinds of nanodiscs on their own, starting with the MSP nanodiscs.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-11fd8c1 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"11fd8c1\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-11e6737\" data-id=\"11e6737\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e7083fc elementor-widget elementor-widget-heading\" data-id=\"e7083fc\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\nMSP Nanodiscs<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-99cca23 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"99cca23\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-b024dc1\" data-id=\"b024dc1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-cc5381f elementor-widget elementor-widget-text-editor\" data-id=\"cc5381f\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><span style=\"color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\">MSP nanodiscs are held together by membrane scaffold proteins (MSPs). MSPs can be truncated forms of apolipoprotein (apo) A-I which wrap around a patch of a lipid bilayer to form a disc-like particle or nanodisc (5). MSPs provide a hydrophobic surface facing the hydrophobic tail of the lipids, and a hydrophilic surface on the outside. This setup makes nanodiscs highly soluble in aqueous solutions. Once assembled into nanodiscs, membrane proteins can be kept in solution in the absence of detergents (5).<\/span><br style=\"color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\" \/><br style=\"color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\" \/><span style=\"font-weight: bolder; color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\">Size:<\/span><span style=\"color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\">\u00a0The size of an MSP nanodisc can range between 7 &#8211; 17 nm. It is determined by the used membrane scaffolding protein. Table 2 depicts the membrane scaffold proteins that Cube Biotech offers and which nanodisc sizes it leads to. MSP nanodiscs of the same MSP protein are uniform in size and only differ +\/- 1 nm in diameter. This suits them perfectly for Cryo-EM studies.<\/span><br style=\"color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\" \/><br style=\"color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\" \/><span style=\"font-weight: bolder; color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\">Other advantages of MSP nanodiscs<\/span><br style=\"color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\" \/><span style=\"color: #222323; font-family: 'titillium web', sans-serif; font-size: 16px; text-align: justify;\">MSP nanodiscs have a number of advantages compared to other systems for membrane protein solubilization and reconstitution, in particular for ligand binding studies, analysis of conformational dynamics, and protein interaction studies (6). Nanodiscs can be used to reconstitute membrane proteins such as GPCRs or transporters in an artificial environment resembling the native membrane.<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-54722f7\" data-id=\"54722f7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-11fbfd0 elementor-widget elementor-widget-image\" data-id=\"11fbfd0\" data-element_type=\"widget\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"969\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.12-845x1024.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.12-845x1024.png 845w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.12-248x300.png 248w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.12-768x930.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.12-495x600.png 495w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.12-132x160.png 132w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.12.png 1146w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-27ab49e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"27ab49e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-9219235\" data-id=\"9219235\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-243cc7e elementor-widget elementor-widget-text-editor\" data-id=\"243cc7e\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>These nanodisc-stabilized proteins can be directly purified by standard chromatographic procedures. The resulting purified membrane protein-nanodisc complex can be used in applications that require access to both the physiologically intracellular and extracellular surfaces of the protein and thus allows unrestricted access to antagonists, agonists, G proteins, and other interaction partners (7).<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-669a4ca elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"669a4ca\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f83e565\" data-id=\"f83e565\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-47c0751 e-transform elementor-widget elementor-widget-image\" data-id=\"47c0751\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"460\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.54-1024x589.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.54-1024x589.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.54-300x172.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.54-768x441.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.54-1536x883.png 1536w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.54-600x345.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.54-278x160.png 278w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.56.54.png 1670w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-bb89d10 elementor-widget elementor-widget-text-editor\" data-id=\"bb89d10\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Figure 4: Schematic image of two ways to reconstitute proteins into nanodiscs. A: Assembled nanodiscs are added to a cell-free reaction. The nascent protein can insert spontaneously. B: Detergent and MSP are added to cells expressing the protein of interest. A complex of membrane phospholipids, proteins, and MSP forms.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-b64237e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"b64237e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-40e46df\" data-id=\"40e46df\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0453801 elementor-widget elementor-widget-text-editor\" data-id=\"0453801\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div class=\"col-12\"><strong>A: Combining nanodiscs and cell-free expression systems<\/strong><br \/>Starting from an expression plasmid, membrane proteins can be produced in cell-free systems. Pre-assembled nanodiscs are supplied in the mixture that integrate the nascent membrane protein (8). The addition of detergents is not required, which minimizes possible artifacts. Optionally, modifications such as biotinylation or isotope labeling can be included.<\/div><div class=\"col-12\"><strong>\u00a0<\/strong><\/div><div class=\"col-12\"><strong>B: Two-step reconstitution of detergent-solubilized proteins<\/strong><br \/>Starting from a purified membrane protein in suitable detergent, membrane scaffold proteins and phospholipids are added. Nanodiscs containing the membrane protein form spontaneously, and can be purified by affinity or size exclusion chromatography (6, 7).<\/div><div class=\"col-12\">\u00a0<\/div><div class=\"col-12\"><strong>C: Direct solubilization from membranes<\/strong><br \/>Starting from membranes expressing the protein of interest, detergent and membrane scaffold protein are added. Membrane phospholipids, membrane protein, and MSP assemble to form the nanodisc complex (5). Here, a mixture of nanodisc complexes representing the membrane protein population is obtained, which may be used for proteomics studies. If required, individual membrane protein-nanodisc complexes can be purified by affinity chromatography. Compared to method B, exposure time to detergents is significantly shorter (hours vs. days).<\/div>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-d54c42c elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"d54c42c\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-f4570c7\" data-id=\"f4570c7\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-ce42fdd elementor-widget elementor-widget-heading\" data-id=\"ce42fdd\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\nChoice of Phospholipids - the Key to proper Protein Activity<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-43fe984 elementor-widget elementor-widget-text-editor\" data-id=\"43fe984\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div>As already mentioned the phospholipids composition of an MSP nanodisc is artificial. Meaning the used phospholipids that should make up the artificial membrane environment for the membrane protein of interest must be decided before. But there are numerous phospholipids to choose from out there, so which to choose? Refer to this list of our most commonly used phospholipids for MSP nanodiscs, when faced with this question.<\/div>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b7ad0b3 e-transform elementor-widget elementor-widget-image\" data-id=\"b7ad0b3\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:0.4,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"757\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.59.43-1024x969.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.59.43-1024x969.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.59.43-300x284.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.59.43-768x727.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.59.43-600x568.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.59.43-169x160.png 169w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-16.59.43.png 1446w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-41472dc elementor-widget elementor-widget-text-editor\" data-id=\"41472dc\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>This selection, but also many other phospholipids have been successfully used alone or in combination (8,25). The choice of lipids has been shown to be crucial for protein activity (8), for example in cases where lipids promote protein oligomerization (25). Cell-free expression using assembled nanodiscs is a fast and easy way to screen a variety of lipids and lipid mixtures for their effect on the protein. When proteins are solubilized directly from the membrane fraction, endogenous phospholipids are carried along and incorporated into the nanodisc complex, which may enhance protein activity.<\/p><h4>\u00a0<\/h4><h5>Examples of MSP nanodisc applications in science<\/h5><div>MSP Nanodiscs were first described by Sligar and coworkers (3,4). They provide the perfect environment to stabilize membrane proteins to study the binding of ligands, agonists, or antagonists by methods such as NMR and SPR (9,10). Nanodiscs were shown to increase the resolution of membrane-spanning protein regions in Cryo-EM (22,26). Membrane scaffold proteins can be tagged with histidines to facilitate purification, detection, and immobilization of the protein-nanodisc complex. Other nanodisc applications include resonance Raman (11), MALDI (13), non-covalent mass spectrometry (25), protein activation studies (14), time-resolved fluorescence spectroscopy (15), and protein crystallization (24). Antigens reconstituted into nanodiscs have been used to raise immunogenic responses in mice, showing their potential to be used as vaccines (16). In addition, the entire membrane proteome of\u00a0<em>E.coli<\/em>\u00a0was reconstituted into nanodiscs, thereby creating a solubilized membrane protein library (15). Proteins reconstituted in nanodiscs can be transferred to bicelles to improve NMR resolution (23). Even soluble, lipid-interacting proteins were analyzed with the help of nanodiscs (20). Table 3 lists examples for nanodisc applications.<\/div>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-fcbb907 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"fcbb907\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-8daa0c4\" data-id=\"8daa0c4\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-b034f98 elementor-widget elementor-widget-heading\" data-id=\"b034f98\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\nMSP Nanodiscs<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-4da12df elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"4da12df\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-dd5f32f\" data-id=\"dd5f32f\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-d428a68 elementor-widget elementor-widget-text-editor\" data-id=\"d428a68\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Synthetic nanodiscs are the second big option in the field of nanodisc. They differ in certain key aspects from their MSP counterparts, but also share certain similarities.<br \/><br \/><strong>Creation of synthetic nanodiscs<\/strong><br \/>In contrast to the three creation ways of MSP nanodiscs (figure 4), synthetic nanodiscs can only be created directly from intact cells. The used synthetic polymer has a dual function during this process. First, it dissolves the cell membrane, similar to a detergent. Then it forms a nanodisc structure around membrane proteins using the native cell phospholipids. A good analogy to this process is a cookie cutter that stamps the cookies out of the dough.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-d84cb5a\" data-id=\"d84cb5a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-73d09cf e-transform elementor-widget elementor-widget-image\" data-id=\"73d09cf\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:0.8,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"542\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.04.15.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.04.15.png 892w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.04.15-300x203.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.04.15-768x520.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.04.15-600x406.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.04.15-236x160.png 236w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-a728023 elementor-widget elementor-widget-text-editor\" data-id=\"a728023\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Figure 5: Schematic depiction of a synthetic nanodisc that stabilizes a hypothetical membrane protein. The figure legend mentions SMA as the used stabilizing polymer, but e.g. DIBMA could fill this role as well.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-c3a0bac elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"c3a0bac\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-461da84\" data-id=\"461da84\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-0860861 elementor-widget elementor-widget-text-editor\" data-id=\"0860861\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><br \/>Synthetic nanodiscs are variable in their size. The main factor that decides their diameter is the size of the membrane protein complex that they surround and stabilize. Therefore a definitive size cannot be given for a synthetic nanodisc. But they all range in the size range that can also be found in MSP nanodiscs (table 2). This applies to all established polymers so far. If uniformous nanodisc size is desired for a synthetic nanodiscs complex, a size-exclusion chromatography (SEC) has to be performed after the stabilized membrane protein of interest has been purified by e. g.<a href=\"https:\/\/cube-biotech.com\/knowledge\/protein-purification\/rho1d4\/\">\u00a0affinity chromatography with the Rho1D4-tag.<\/a><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-dbd178e elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"dbd178e\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-5232745\" data-id=\"5232745\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-15f3493 elementor-widget elementor-widget-text-editor\" data-id=\"15f3493\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><strong id=\"dibmaorsma\">Which polymer?<\/strong><br \/>The selection of different polymers for synthetic nanodiscs is constantly growing. Each with its own benefits and downsides.\u00a0DIBMA\u00a0for example has protein-like absorption at a wavelength of 280 nm. Meanwhile,\u00a0AASTY\u00a0has quite fixed nanodiscs diameters compared to other polymers.<br \/><br \/>However, this is only a small extract from a long list of pros and cons of the different polymers. We dedicated this topic its own webpage.\u00a0Have a look at it!<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-50 elementor-top-column elementor-element elementor-element-0dd95c9\" data-id=\"0dd95c9\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-fabfdfc e-transform elementor-widget elementor-widget-image\" data-id=\"fabfdfc\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:0.8,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"273\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.06.45-1024x350.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.06.45-1024x350.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.06.45-300x103.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.06.45-768x262.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.06.45-600x205.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.06.45-280x96.png 280w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.06.45.png 1270w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cc96707 elementor-widget elementor-widget-text-editor\" data-id=\"cc96707\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Figure 6: DIBMA and SMA seem to be interchangeable in the first view, but there are some differences.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-22475e7 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"22475e7\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6a9354a\" data-id=\"6a9354a\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a06895d elementor-widget elementor-widget-heading\" data-id=\"a06895d\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h2 class=\"elementor-heading-title elementor-size-default\">\nMSP or Synthetic Nanodiscs?<\/h2>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-168906b elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"168906b\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-51492b2\" data-id=\"51492b2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-96ebc29 elementor-widget elementor-widget-text-editor\" data-id=\"96ebc29\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<div>So after all of this, the question remains what type of nanodisc best suits your project? Both MSP and synthetic nanodiscs are meant for the solubilization &amp; stabilization of membrane proteins by mimicking a cell-membrane environment. However, as mentioned before, there are some key differences between the two. Table 4 lists all differences and their respective advantages &amp; disadvantages.<\/div><div><br \/>Table 4: Direct comparison between MSP and Synthetic nanodiscs.<\/div>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ac11827 e-transform elementor-widget elementor-widget-image\" data-id=\"ac11827\" data-element_type=\"widget\" data-settings=\"{&quot;_transform_scale_effect&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:1,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_tablet&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]},&quot;_transform_scale_effect_mobile&quot;:{&quot;unit&quot;:&quot;px&quot;,&quot;size&quot;:&quot;&quot;,&quot;sizes&quot;:[]}}\" data-widget_type=\"image.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img width=\"800\" height=\"337\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.08.46-1024x431.png\" class=\"attachment-large size-large\" alt=\"\" loading=\"lazy\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.08.46-1024x431.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.08.46-300x126.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.08.46-768x323.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.08.46-1536x647.png 1536w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.08.46-2048x862.png 2048w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.08.46-600x253.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/04\/Screenshot-2023-04-03-at-17.08.46-280x118.png 280w\" sizes=\"(max-width: 800px) 100vw, 800px\" \/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-58dbd88 elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"58dbd88\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-6bcb4c1\" data-id=\"6bcb4c1\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-a011fff elementor-widget elementor-widget-spacer\" 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elementor-icon-list--layout-traditional elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list\" data-id=\"ee2b1ca\" data-element_type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<link rel=\"stylesheet\" href=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/plugins\/elementor\/assets\/css\/widget-icon-list.min.css\">\t\t<ul class=\"elementor-icon-list-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/nuevo22.cidsamexico.com\/\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Home<\/span>\n\t\t\t\t\t\t\t\t\t\t\t<\/a>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<a href=\"https:\/\/nuevo22.cidsamexico.com\/index.php\/acerca-de\/\">\n\n\t\t\t\t\t\t\t\t\t\t\t<span 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data-id=\"c76b203\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-665a492 elementor-widget elementor-widget-spacer\" data-id=\"665a492\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-4cb355d elementor-widget elementor-widget-heading\" data-id=\"4cb355d\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Laboratorios y oficinas<\/h5>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-3cb8e22 elementor-list-item-link-inline elementor-mobile-align-center elementor-icon-list--layout-traditional elementor-widget elementor-widget-icon-list\" data-id=\"3cb8e22\" data-element_type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<ul class=\"elementor-icon-list-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Oficinas y Almac\u00e9n en CDMX<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Laboratorios en Zacatecas<\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-text\">Matriz y Laboratorios en Queretaro <\/span>\n\t\t\t\t\t\t\t\t\t<\/li>\n\t\t\t\t\t\t<\/ul>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ff48eb1 elementor-widget elementor-widget-spacer\" data-id=\"ff48eb1\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t<div class=\"elementor-column elementor-col-20 elementor-top-column elementor-element elementor-element-1ef747b\" data-id=\"1ef747b\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e91f201 elementor-widget elementor-widget-spacer\" data-id=\"e91f201\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-f59f720 elementor-widget elementor-widget-heading\" data-id=\"f59f720\" data-element_type=\"widget\" data-widget_type=\"heading.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<h5 class=\"elementor-heading-title elementor-size-default\">Contacto<\/h5>\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-125d7a1 elementor-mobile-align-center elementor-icon-list--layout-traditional elementor-list-item-link-full_width elementor-widget elementor-widget-icon-list\" data-id=\"125d7a1\" data-element_type=\"widget\" data-widget_type=\"icon-list.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<ul class=\"elementor-icon-list-items\">\n\t\t\t\t\t\t\t<li class=\"elementor-icon-list-item\">\n\t\t\t\t\t\t\t\t\t\t\t<span class=\"elementor-icon-list-icon\">\n\t\t\t\t\t\t\t<i aria-hidden=\"true\" class=\"fab fa-whatsapp\"><\/i>\t\t\t\t\t\t<\/span>\n\t\t\t\t\t\t\t\t\t\t<span 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\/>\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-cc8095d elementor-widget elementor-widget-spacer\" data-id=\"cc8095d\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-2da909d elementor-widget__width-initial elementor-absolute elementor-widget elementor-widget-text-editor\" data-id=\"2da909d\" data-element_type=\"widget\" data-settings=\"{&quot;_position&quot;:&quot;absolute&quot;}\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p>Somos una empresa y laboratorio con amplia experiencia. Con\u00f3cenos.<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-b79930a elementor-grid-3 elementor-shape-rounded e-grid-align-center elementor-invisible elementor-widget elementor-widget-social-icons\" data-id=\"b79930a\" data-element_type=\"widget\" data-settings=\"{&quot;_animation&quot;:&quot;zoomIn&quot;}\" data-widget_type=\"social-icons.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t<style>\/*! elementor - v3.7.8 - 02-10-2022 *\/\n.elementor-widget-social-icons.elementor-grid-0 .elementor-widget-container,.elementor-widget-social-icons.elementor-grid-mobile-0 .elementor-widget-container,.elementor-widget-social-icons.elementor-grid-tablet-0 .elementor-widget-container{line-height:1;font-size:0}.elementor-widget-social-icons:not(.elementor-grid-0):not(.elementor-grid-tablet-0):not(.elementor-grid-mobile-0) .elementor-grid{display:inline-grid}.elementor-widget-social-icons 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.elementor-icon.elementor-social-icon{border-radius:10%}.elementor-shape-circle .elementor-icon.elementor-social-icon{border-radius:50%}<\/style>\t\t<div class=\"elementor-social-icons-wrapper elementor-grid\">\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-facebook elementor-repeater-item-da64bc7\" href=\"http:\/\/www.facebook.com\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Facebook<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-facebook\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-twitter elementor-repeater-item-0b93d4e\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Twitter<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-twitter\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t\t\t<span class=\"elementor-grid-item\">\n\t\t\t\t\t<a class=\"elementor-icon elementor-social-icon elementor-social-icon-linkedin elementor-repeater-item-50b061f\" target=\"_blank\">\n\t\t\t\t\t\t<span class=\"elementor-screen-only\">Linkedin<\/span>\n\t\t\t\t\t\t<i class=\"fab fa-linkedin\"><\/i>\t\t\t\t\t<\/a>\n\t\t\t\t<\/span>\n\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<div class=\"elementor-element elementor-element-ff2092c elementor-widget elementor-widget-spacer\" data-id=\"ff2092c\" data-element_type=\"widget\" data-widget_type=\"spacer.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t<div class=\"elementor-spacer\">\n\t\t\t<div class=\"elementor-spacer-inner\"><\/div>\n\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-2e3a91f elementor-section-boxed elementor-section-height-default elementor-section-height-default\" data-id=\"2e3a91f\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-7f3a0c2\" data-id=\"7f3a0c2\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t\t\t<div class=\"elementor-element elementor-element-e27f8c2 elementor-widget elementor-widget-text-editor\" data-id=\"e27f8c2\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p style=\"text-align: center;\"><span style=\"font-size: 9px; text-align: center;\">Copyright \u00a9 2023 CIDSA Mexico. All rights reserved<\/span><\/p><p style=\"text-align: center;\"><span style=\"font-size: 9px; text-align: center;\">Dise\u00f1ado por Suguza Publicidad<\/span><\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>What Are Nanodiscs? The term nanodiscs describes a small (7-50 nm in diameter) disc-shaped structure that finds use in proteomics and biomedicine. It consists of two main components: Phospholipids,\u00a0either of the artificial origin or from the cell membrane A\u00a0stabilizing belt\u00a0that holds the phospholipids together. This is either an MSP protein or a\u00a0synthetic polymer. \u00a0 \u00a0 [&hellip;]<\/p>\n","protected":false},"author":3,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"elementor_header_footer","meta":{"om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0},"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/pages\/10161"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/comments?post=10161"}],"version-history":[{"count":14,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/pages\/10161\/revisions"}],"predecessor-version":[{"id":10189,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/pages\/10161\/revisions\/10189"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=10161"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}