{"id":9619,"date":"2023-03-27T21:12:44","date_gmt":"2023-03-28T03:12:44","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9619"},"modified":"2023-03-27T21:48:55","modified_gmt":"2023-03-28T03:48:55","slug":"ace2-receptor-ectodomain","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ace2-receptor-ectodomain\/","title":{"rendered":"ACE2 Receptor (Ectodomain)"},"content":{"rendered":"\n

ACE2 (enzima convertidora de angiotensina 2) es la puerta de entrada del coronavirus SARS-CoV-2 a nuestras c\u00e9lulas. Su funci\u00f3n celular real es la de una carboxipeptidasa unida a la superficie celular. Le proporcionamos el Ectodominio de ACE2, listo para estudios de uni\u00f3n de ligandos, cristalizaci\u00f3n y otros experimentos bioqu\u00edmicos\/biof\u00edsicos.<\/p>\n\n\n\n

Si est\u00e1 interesado en el receptor ACE2 de longitud completa, comun\u00edquese con nuestros servicios de prote\u00ednas. O utilice el formulario de contacto para ponerse en comunicaci\u00f3n con nosotros.<\/p>\n\n\n\n

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Figure 1:<\/strong> ACE2 and its role in the SARC-CoV-2 infection.<\/figcaption><\/figure>\n\n\n\n
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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
CU-28621<\/td>ACE2 Receptor (Ectodomain)<\/td>25 ug<\/td><\/tr>
CU-28621-1<\/td>ACE2 Receptor (Ectodomain)<\/td>4 x 25 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Datasheet <\/a><\/p>\n<\/div>\n\n\n\n

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Caracteristicas<\/mark><\/p>\n\n\n\n

Alternative names<\/td>ACE2 (Angiotensin-converting enzyme 2)<\/td><\/tr>
UniProt number<\/td>Q9BYF1<\/td><\/tr>
Protein class<\/td>Single span transmembrane protein, Ectodomain<\/td><\/tr>
Organism<\/td>Human (Homo sapiens)<\/td><\/tr>
Sequence, explained<\/td>Ectodomain (aa 1 \u2013 740), including signal peptide, 6 x His tag fused to C-terminus<\/td><\/tr>
Sequence, One-Letter Code<\/td>MSSSSWLLLS LVAVTAAQST IEEQAKTFLD KFNHEAEDLF
YQSSLASWNY NTNITEENVQ NMNNAGDKWS AFLKEQSTLA
QMYPLQEIQN LTVKLQLQAL QQNGSSVLSE DKSKRLNTIL
NTMSTIYST GKVCNPDNPQ ECLLLEPGLN EIMANSLDYN
ERLWAWESWR SEVGKQLRPL YEEYVVLKN EMARANHYED
YGDYWRGDYE VNGVDGYDYS RGQLIEDVEH TFEEIKPLYE
HLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTNL
YSLTVPFGQKP NIDVTDAMVD QAWDAQRIF KEAEKFFVSV
GLPNMTQGFWE NSMLTDPGNV QKAVCHPTAW DLGKGDFRIL
MCTKVTMDD FLTAHHEMGH IQYDMAYAAQP FLLRNGANEG
FHEAVGEIMS LSAATPKHLK SIGLLSPDF QEDNETEINF
LLKQALTIVG TLPFTYMLE KWRWMVFKG EIPKDQWMKK
WWEMKREIV GVVEPVPHD ETYCDPASLF HVSNDYSFIR
YYTRTLYQFQ FQEALCQAA KHEGPLHKC DISNSTEAGQ
KLFNMLRLGK SEPWTLALE NVVGAKNMNV RPLLNYFEPL
FTWLKDQNK NSFVGWSTDW SPYADQSIKV RISLKSALGD
KAYEWNDNEM YLFRSSVAYA MRQYFLKVKN QMILFGEED
VRVANLKPRI SFNFFVTAPK NVSDIIPRTE VEKAIRMSRS
RINDAFRLND NSLEFLGIQ PTLGPPNQPP VSHHHHHH<\/td><\/tr>
Affinity tag<\/td>C-terminal 6x His tag without spacer<\/td><\/tr>
Expression Host<\/td>Hek293 Expi cells<\/td><\/tr>
Size<\/td>746 amino acids (including 6 x His tag)
86,1 kDa<\/td><\/tr>
Buffer composition<\/td>20 mM Hepes pH 7.4; 100 mM NaCl<\/td><\/tr>
Function<\/td>Host cell receptor for virus entry, Protease<\/td><\/tr>
Preparation<\/td>Expression system:<\/em> Hek293 Expi cellsPurification:<\/em> Affinity purification (INDIGO Agarose) and size exclusion chromatographyForm:<\/em> LiquidBuffer composition:<\/em> 20 mM Hepes pH 7.4; 100 mM NaCl<\/td><\/tr>
Quality Control<\/td>Purity:<\/em> >95% as determined by SDS-PAGEHomogeneity:<\/em> Size Exclusion ChromatographyActivity:<\/em> not determined<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Aplicaciones<\/mark>
\u2022 ELISA Assays
\u2022 Biochemical and biophysical analyses
\u2022 Ligand binding assays (e.g. SPR)<\/p>\n\n\n\n

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Envio y Almacenamiento<\/mark><\/p>\n\n\n\n

Este producto se envia en hielo seco y se almacena a -80\u00b0C. Evitar ciclos de congelamiento y descongelamiento.<\/p>\n\n\n\n

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Imagenes<\/mark><\/p>\n\n\n\n

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Figure 2:<\/strong> SDS-PAGE and subsequent western blot showing the Ectodomain of ACE2. The His-tagged protein was purified using INDIGO-Ni agarose<\/a>.<\/figcaption><\/figure>\n\n\n\n
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Figure 3:<\/strong> The ACE2 ectodomain was immobilized at a concentration of 500 ng per well, the trimeric CoV-2 spike protein was used in triplicates at concentrations between 1 x 10-9M and 2 x 10-5M<\/sup>. The apparent KD value is approx. 2x 10-8M<\/sup>.<\/figcaption><\/figure>\n\n\n\n
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Descargo de responsabilidad<\/mark>:<\/strong> Nuestros productos est\u00e1n destinados para investigacion en aplicaciones de biolog\u00eda molecular. Estos productos no est\u00e1n destinados al diagn\u00f3stico, prevenci\u00f3n o tratamiento de una enfermedad.<\/p>\n\n\n\n

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