{"id":9615,"date":"2023-03-27T19:28:41","date_gmt":"2023-03-28T01:28:41","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9615"},"modified":"2023-03-27T19:52:02","modified_gmt":"2023-03-28T01:52:02","slug":"proteina-achr-t-californica","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/proteina-achr-t-californica\/","title":{"rendered":"Proteina AChR T. Californica"},"content":{"rendered":"\n

ProteinsOur AChR se purifica directamente del receptor de acetilcolina de Torpedo californica. Es el mejor ejemplo de c\u00f3mo se pueden usar perlas de purificaci\u00f3n de prote\u00ednas personalizadas para aislar una prote\u00edna sin ninguna etiqueta de ep\u00edtopo. La prote\u00edna se purific\u00f3 mediante resina de agarosa acoplada a neurotoxina-3 de cobra real, un ligando natural de AChR.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
CU-28601<\/td>Proteina AChR T. Californica<\/td>40 ug<\/td><\/tr>
CU-28603<\/td>Proteina AChR T. Californica<\/td>80 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

Datasheet<\/a> <\/p>\n<\/div>\n\n\n\n

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Caracteristicas<\/mark><\/p>\n\n\n\n

Alternative names<\/td>Acetylcholine receptor subunit alpha, beta, gamma and delta<\/td><\/tr>
UniProt Number<\/td>P02712 (alpha subunit)<\/a>P02714 (beta subunit)<\/a>P02718 (gamma subunit)<\/a>P02710 (delta subunit)<\/a><\/td><\/tr>
Protein class<\/td>Ion channel, Ligand-gated ion channel, Receptor<\/td><\/tr>
Organism<\/td>Torpedo californica<\/em> (electric ray)<\/td><\/tr>
Sequence, Alpha subunit One-Letter Code<\/td>MILCSYWHV GLVLLLFSCC GLVLGSEHET RLVANLLENY NKVIRPVEHH THFVDITVGL QLIQLISVDE VNQIVETNVR LRQQWIDVRL RWNPADYGGI KKIRLPSDDV WLPDLVLYNN ADGDFAIVHM TKLLLDYTGK IMWTPPAIFK SYCEIIVTHF PFDQQNCTMK LGIWTYDGTK VSISPESDRP DLSTFMESGE WVMKDYRGWK HWVYYTCCPD TPYLDITYHF IMQRIPLYFV VNVIIPCLLF SFLTGLVFYL PTDSGEKMTL SISVLLSLTV FLLVIVELIP STSSAVPLIG KYMLFTMIFV ISSIIITVVV INTHHRSPST HTMPQWVRKI FIDTIPNVMF FSTMKRASKE KQENKIFADD IDISDISGKQ VTGEVIFQTP LIKNPDVKSA IEGVKYIAEH MKSDEESSNA AEEWKYVAMV IDHILLCVFM LICIIGTVSV FAGRLIELSQ EG<\/td><\/tr>
Sequence, Beta subunit One-Letter Code<\/td>MENVRRMALG LVVMMALALS GVGASVMEDT LLSVLFETYN PKVRPAQTVG DKVTVRVGLT LTNLLILNEK IEEMTTNVFL NLAWTDYRLQ WDPAAYEGIK DLRIPSSDVW QPDIVLMNNN DGSFEITLHV NVLVQHTGAV SWQPSAIYRS SCTIKVMYFP FDWQNCTMVF KSYTYDTSEV TLQHALDAKG EREVKEIVIN KDAFTENGQW SIEHKPSRKN WRSDDPSYED VTFYLIIQRK PLFYIVYTII PCILISILAI LVFYLPPDAG EKMSLSISAL LAVTVFLLLL ADKVPETSLS VPIIIRYLMF IMILVAFSVI LSVVVLNLHH RSPNTHTMPN WIRQIFIETL PPFLWIQRPV TTPSPDSKPT IISRANDEYF IRKPAGDFVC PVDNARVAVQ PERLFSEMKW HLNGLTQPVT LPQDLKEAVE AIKYIAEQLE SASEFDDLKK DWQYVAMVAD RLFLYVFFVI CSIGTFSIFL DASHNVPPDN PFA<\/td><\/tr>
Sequence, Gamma subunit One-Letter Code<\/td>MVLTLLLIIC LALEVRSENE EGRLIEKLLG DYDKRIIPAK TLDHIIDVTL KLTLTNLISL NEKEEALTTN VWIEIQWNDY RLSWNTSEYE GIDLVRIPSE LLWLPDVVLE NNVDGQFEVA YYANVLVYND GSMYWLPPAI YRSTCPIAVT YFPFDWQNCS LVFRSQTYNA HEVNLQLSAE EGEAVEWIHI DPEDFTENGE WTIRHRPAKK NYNWQLTKDD TDFQEIIFFL IIQRKPLFYI INIIAPCVLI SSLVVLVYFL PAQAGGQKCT LSISVLLAQT IFLFLIAQKV PETSLNVPLI GKYLIFVMFV SMLIVMNCVI VLNVSLRTPN THSLSEKIKH LFLGFLPKYL GMQLEPSEET PEKPQPRRRS SFGIMIKAEE YILKKPRSEL MFEEQKDRHG LKRVNKMTSD IDIGTTVDLY KDLANFAPEI KSCVEACNFI AKSTKEQNDS GSENENWVLI GKVIDKACFW IALLLFSIGT LAIFLTGHFN QVPEFPFPGD PRKYVP<\/td><\/tr>
Sequence, Delta subunit One-Letter Code<\/td>MGNIHFVYLLISCLYYSGCS GVNEEERLIN DLLIVNKYNK HVRPVKHNNE VVNIALSLTL SNLISLKETD ETLTSNVWMD HAWYDHRLTW NASEYSDISI LRLPPELVWI PDIVLQNNND GQYHVAYFCN VLVRPNGYVT WLPPAIFRSS CPINVLYFPF DWQNCSLKFT ALNYDANEIT MDLMTDTIDG KDYPIEWIII DPEAFTENGE WEIIHKPAKK NIYPDKFPNG TNYQDVTFYL IIRRKPLFYV INFITPCVLI SFLASLAFYL PAESGEKMST AISVLLAQAV FLLLTSQRLP ETALAVPLIG KYLMFIMSLV TGVIVNCGIV LNFHFRTPST HVLSTRVKQI FLEKLPRILH MSRADESEQP DWQNDLKLRR SSSVGYISKA QEYFNIKSRS ELMFEKQSER HGLVPRVTPR IGFGNNNENI AASDQLHDEI KSGIDSTNYI VKQIKEKNAY DEEVGNWNLV GQTIDRLSMF IITPVMVLGT IFIFVMGNFN HPPAKPFEGD PFDYSSDHPR CA<\/td><\/tr>
Affinity tags<\/td>none<\/td><\/tr>
Expression Host<\/td>T. californica <\/em>(native)<\/td><\/tr>
Size (excluding additional elements)<\/td>65 kDA<\/td><\/tr>
Buffer composition<\/td>137 mM NaCl, 2.7 mM KCl, 10 mM Na2<\/sub>HPO4<\/sub>, 1.8 mM KH2<\/sub>PO4<\/sub>, 5 % glycerin; 0,1 % Triton<\/td><\/tr>
Purity<\/td>>95%, determined via SDS-PAGE<\/td><\/tr>
Purified via<\/td>Protein-specific affinity matrix using immobilized agonist ligand <\/a>followed by dialysis<\/td><\/tr>
Function<\/td>After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Preparaci\u00f3n<\/mark>
Expression system:<\/strong> T. Californica (native)
Purification:<\/strong> Protein-specific affinity matrix using immobilized agonist ligand, dialysis
Buffer: <\/strong> 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 5 % glycerin;
0,1 % Triton
Activity:<\/strong> Native AChR from T.Californica, purified as funtional heterotetramer binding to
Neurotoxin-3 ligand
Form: <\/strong> Liquid<\/p>\n\n\n\n

<\/div>\n\n\n\n

Control de Calidad<\/mark>
Purity:<\/strong> (SDS-PAGE) >95% as determined by SDS-PAGE, see Fig. 1
Activity: <\/strong> Purified as funtional heterotetramer binding to Neurotoxin-3 ligand<\/p>\n\n\n\n

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Aplicaciones<\/mark>
\u2022 SDS-PAGE
\u2022 Western Blot
\u2022 Ligand binding assays (e.g. SPR)
\u2022 Protein Crystallization
\u2022 Biochemical and biophysical analyses
\u2022 Immunization<\/p>\n\n\n\n

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Envio y Almacenamiento<\/mark><\/p>\n\n\n\n

Este producto se envia en hielo seco y se almacena a -80\u00b0C. Evitar ciclos de congelamiento y descongelamiento.<\/p>\n\n\n\n

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Imagenes<\/mark><\/p>\n\n\n\n

<\/p>\n\n\n\n

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Figure 1:<\/strong>\u00a0Western blot of purified AchR showing all four domains at the expected sizes<\/figcaption><\/figure>\n\n\n\n
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Descargo de responsabilidad<\/mark>:<\/strong> Nuestros productos est\u00e1n destinados para investigacion en aplicaciones de biolog\u00eda molecular. Estos productos no est\u00e1n destinados al diagn\u00f3stico, prevenci\u00f3n o tratamiento de una enfermedad.<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Proteina de Cube Biotech<\/p>\n","protected":false},"featured_media":9617,"template":"","al_product-cat":[39,34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9615"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":1,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9615\/revisions"}],"predecessor-version":[{"id":9618,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9615\/revisions\/9618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9617"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9615"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9615"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}