{"id":9592,"date":"2023-03-27T14:16:46","date_gmt":"2023-03-27T20:16:46","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9592"},"modified":"2023-03-27T18:28:30","modified_gmt":"2023-03-28T00:28:30","slug":"proteina-glp1r","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/proteina-glp1r\/","title":{"rendered":"Proteina Channelrhodopsin-1 (Ca)"},"content":{"rendered":"\n

La canalrodopsina 1 es una prote\u00edna de 7 transmembranas que act\u00faa como un canal cati\u00f3nico impulsado por la luz en las algas verdes eucariotas. Debido a su ligando coloreado unido covalentemente, es ideal para usar como prote\u00edna de membrana de control, por ejemplo, para ensayos de cristalizaci\u00f3n y experimentos bioqu\u00edmicos\/biof\u00edsicos. Proporcionamos canalrodopsina 1 de alta pureza, purificada de levadura como prote\u00edna etiquetada con His.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
CU-28941<\/td>Proteina Channelrhodopsin-1 (ca)<\/td>100 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Datasheet<\/a> <\/p>\n<\/div>\n\n\n\n

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Caracteristicas<\/mark><\/p>\n\n\n\n

Alternative names<\/td>Channelrhodopsin 1 (CaChR1)<\/td><\/tr>
UniProt Number<\/td>G8HKA1<\/a><\/td><\/tr>
Protein class<\/td>7-transmembrane protein<\/td><\/tr>
Organism<\/td>Chloromonas augustae (Chlamydomonas augustae)<\/em><\/td><\/tr>
Sequence, explained<\/td>wild-type sequence, N-terminal membrane-spanning domain (362 of 715 aa), 10x His-tag<\/td><\/tr>
Sequence, One-Letter Code<\/td>MDTLAWVARE LLSTAHDATP ATATPSTDHS TPSTDHGSGE TFNVTITIGG GHHGGHAGPV DNSIVIGGID GWIAIPAGDC YCAGWYVSHG SSFEATFAHV CQWSIFAVCI LSLLWYAWQY WKATCGWEEV YVCCIELVFI CFELYHEFDS PCSLYLSTAN IVNWLRYSEW LLCCPVILIH LSNVTGLSDD YGRRTMGLLV SDIATIVFGI TAAMLVSWPK IIFYLLGFTM CCYTFYLAAK VLIESFHQVP KGICRHLVKA MAITYYVGWS FFPLIFLFGQ SGFKKISPYA DVIASSFGDL ISKNMFGLLG HFLRVKIHEH ILKHGDIRKT THLRIAGEEK EVETFVEEED EDHHHHHHHH HH<\/td><\/tr>
Affinity tags<\/td>His-tag (C-terminus)<\/td><\/tr>
Expression Host<\/td>Pichia pastoris (yeast)<\/em><\/td><\/tr>
Size<\/td>362 amino acids; 40,661 Da<\/td><\/tr>
Buffer composition<\/td>100 mM NaCl, 20 mM HEPES pH 7.4, 0.03% dodecyl maltoside (DDM)<\/td><\/tr>
Purity<\/td>>98%, determined via SDS-PAGE (Figure 1)<\/td><\/tr>
Purified via<\/td>PureCube Ni-NTA Agarose<\/a><\/td><\/tr>
Absorbance<\/td>Extinction coefficient at 518 nm: 36,000 M-1<\/sup> cm-1<\/sup>..
Extinction coefficient at 590 nm: 54,000 M-1<\/sup> cm-1<\/sup>.<\/td><\/tr>
Activity<\/td>Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm \/ 518 nm = 2.2 (additional vibronic band at 480 nm)<\/td><\/tr>
Function<\/td>Photoreceptor protein undergoing a photocycle, Retinal protein, light-driven cation channel<\/td><\/tr>
Literature references<\/td>Hou, Sing\u2010Yi, et al. “Diversity of Chlamydomonas channelrhodopsins.” Photochemistry and photobiology 88.1 (2012): 119-128.<\/a>Muders, Vera, et al. “Resonance Raman and FTIR spectroscopic characterization of the closed and open states of channelrhodopsin-1.” FEBS letters 588.14 (2014): 2301-2306.<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n<\/mark><\/p>\n\n\n\n


Expression system<\/strong>: Pichia pastoris (yeast)
Purification<\/strong>: PureCube Ni-NTA Agarose, size exclusion chromatography
Buffer:<\/strong> 100 mM NaCl, 20 mM MES pH 7.4, 0.03% dodecyl maltoside (DDM)
Concentration<\/strong>: 0.5 mg\/mL <\/p>\n\n\n\n

Volume<\/strong>: 205 \u00b5L per 100 \u00b5g aliquot<\/p>\n\n\n\n

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Control de Calidad<\/mark>
Purity: :<\/strong> >98%, as determined by SDS-PAGE, See Fig. 1
Activity:<\/strong> Binding of ligand all-trans retinal, covalently bound to a lysine residue.
Evaluation of UV-VIS spectrum: absorbance ratio 280 nm \/ 518 nm = 2.2, additional
vibronic band at 480 nm, see Figure 2<\/p>\n\n\n\n

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Aplicaciones<\/mark>
\u2022 SDS-PAGE
\u2022 Western Blot
\u2022 Protein Crystallization
\u2022 Biochemical and biophysical analyses<\/p>\n\n\n\n

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Envio y Almacenamiento<\/mark><\/p>\n\n\n\n

Este producto se envia en hielo seco y se almacena a -80\u00b0C. Evitar los ciclos de congelaci\u00f3n y descongelaci\u00f3n. <\/p>\n\n\n\n

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Imagenes<\/mark><\/p>\n\n\n\n

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Figure 1:<\/strong> SDS-PAGE of channelrhodopsin 1 from C. augustae.<\/em><\/figcaption><\/figure>\n\n\n\n
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Figure 2:<\/strong> Absorbance spectrum of channelrhodopsin 1 from C. augustae.<\/em><\/figcaption><\/figure>\n\n\n\n
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Descargo de responsabilidad<\/mark>:<\/strong> Nuestros productos est\u00e1n destinados para investigacion en aplicaciones de biolog\u00eda molecular. Estos productos no est\u00e1n destinados al diagn\u00f3stico, prevenci\u00f3n o tratamiento de una enfermedad.<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Proteina de Cube Biotech<\/p>\n","protected":false},"featured_media":9605,"template":"","al_product-cat":[39,34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9592"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9592\/revisions"}],"predecessor-version":[{"id":9607,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9592\/revisions\/9607"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9605"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9592"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9592"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}