{"id":9587,"date":"2023-03-27T13:08:34","date_gmt":"2023-03-27T19:08:34","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9587"},"modified":"2023-03-27T14:28:27","modified_gmt":"2023-03-27T20:28:27","slug":"proteina-gpbar1-tgr5","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/proteina-gpbar1-tgr5\/","title":{"rendered":"Proteina GPBAR1 (TGR5)"},"content":{"rendered":"\n

Las prote\u00ednas de membrana son la clase de prote\u00ednas m\u00e1s relevante desde el punto de vista farmac\u00e9utico. Al mismo tiempo, es muy dif\u00edcil obtenerlos en forma pura y activa. Los expertos en prote\u00ednas de Cube Biotech han trabajado arduamente para producir GPCR humanos con la calidad suficiente que ahora podemos ofrecer a la comunidad cientifica.<\/p>\n\n\n\n

Nuestra primera prote\u00edna de membrana activa pura disponible es GPBAR1\/TGR5, un GPCR humano involucrado en la homeostasis de los \u00e1cidos biliares, el metabolismo de la glucosa y el mantenimiento del peso. La prote\u00edna est\u00e1 disponible lista para usar, lista para estudios de uni\u00f3n de ligandos, cristalizaci\u00f3n y otros experimentos bioqu\u00edmicos\/biof\u00edsicos.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
CU-28101<\/td>Proteina GPBAR1 (TGR5)<\/td>10 ug<\/td><\/tr>
CU-28103<\/td>Proteina GPBAR1 (TGR5)<\/td>50 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Datasheet GPBAR1 – TGR5<\/a><\/p>\n<\/div>\n\n\n\n

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Caracteristicas<\/mark><\/p>\n\n\n\n

Alternative names<\/td>GPBAR-1,TGR5, GPCR19, M-BAR, BG37<\/td><\/tr>
UniProt \/ UniGene<\/td>Q8TDU6 \/ 155918<\/td><\/tr>
Protein class<\/td>GPCR, class A (Rhodopsin-like receptors)<\/td><\/tr>
Organism<\/td>Human (Homo sapiens)<\/td><\/tr>
Sequence, explained<\/td>Full-length, wildtype sequence N-terminal HA signal sequence, 10x His-tag with spacer, HRV 3C protease site spacer, Rho1D4 tag<\/td><\/tr>
Sequence, One-Letter Code<\/td>MKTIIA LSYIFCLVFA MTPNSTGEVP SPIPKGALGL
SLALASLIIT ANLLLALGIA WDRRLRSPPA GCFFLSLLLA
GLLTGLALPT LPGLWNQSRR GYWSCLLVYL APNFSFLSLL
ANLLLVHGER YMAVLRPLQP PGSIRLALLL TWAGPLLFAS
LPALGWNHWT PGANCSSQAI FPAPYLYLEV YGLLLPAVGA
AAFLSVRVLA TAHRQLQDIC RLERAVCRDE PSALARALTW
RQARAQAGAM LLFGLCWGPY VATLLLSVLA YEQRPPLGPG
TLLSLLSLGS ASAAAVPVAM GLGDQRYTAP WRAAAQRCLQ
GLWGRASRDS PGPSIAYHPS SQSSVDLDLN GGHHHHHHHH
HHLEVLFQGP GSSGTETSQV APA<\/td><\/tr>
Affinity tags<\/td>His-tag and Rho1D4-tag (both C-terminal)<\/td><\/tr>
Expression Host<\/td>SF9 (baculovirus)<\/td><\/tr>
Size (excluding additional elements)<\/td>379 (330) amino acids 40,606 (35,248) Da<\/td><\/tr>
Buffer composition<\/td>0 mM NaCl, 20 mM NaH2<\/sub>PO4<\/sub> pH 7.4, 0.1% Foscholine-12, 0.01% cholesterolhemisuccinate (CHS)<\/td><\/tr>
Purity<\/td>>98%, determined via SDS-PAGE<\/td><\/tr>
Homogeneity<\/td>Size exclusion chromatography<\/td><\/tr>
Activity<\/td>Ligand binding measured by SPR using Taurolithocholic Acid Sulfate Disodium Salt (CAS 64936-83-0) at 10\u00b0C. Using a 1:1 binding model, the dissociation constant (Kd) was determined to be 1.1x10e-8 M<\/sup>.<\/td><\/tr>
Function<\/td>Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids, and in modulating inflammation.<\/td><\/tr>
Literature references<\/td>Kawamata, Yuji, et al. “AG protein-coupled receptor responsive to bile acids.” Journal of Biological Chemistry 278.11 (2003): 9435-9440.<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n<\/mark><\/p>\n\n\n\n


Expression system<\/strong>: Sf9 (Baculovirus)
Purification<\/strong>: PureCube Rho1D4 Agarose, Size exclusion chromatography
Buffer:<\/strong> 100 mM NaCl, 20 mM NaH2PO4 pH 7.4, 0.1% Foscholine-12, 0.01% cholesterolhemisuccinate CHS)
Form<\/strong>: Liquid<\/p>\n\n\n\n

<\/div>\n\n\n\n

Control de Calidad<\/mark>
Pureza: (SDS-PAGE):<\/strong> >98%, ver Fig. 1
Homogeneidad: <\/strong> Tama\u00f1o exclusi\u00f3n a cromatograf\u00eda
Actividad:<\/strong> Uni\u00f3n del ligando medida por resonancia de plasm\u00f3n superficial (SPR=
usando sal dis\u00f3dica de sulfato de \u00e1cido taurolitoc\u00f3lico (CAS 64936-83-0)
Utilizando un modelo de uni\u00f3n 1:1, se determin\u00f3 la constante de disociaci\u00f3n (Kd) para
ser 1,1 x 10-8 M (Fig.2).<\/p>\n\n\n\n

<\/div>\n\n\n\n

Aplicaciones<\/mark>
\u2022 SDS-PAGE
\u2022 Western Blot
\u2022 Protein Crystallization
\u2022 Biochemical and biophysical analyses<\/p>\n\n\n\n

\u2022 Ligand binding assays (e.g. SPR)<\/p>\n\n\n\n

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Envio y Almacenamiento<\/mark><\/p>\n\n\n\n

Este producto se envia en hielo seco y se almacena a -80\u00b0C. Evitar los ciclos de congelaci\u00f3n y descongelaci\u00f3n. <\/p>\n\n\n\n

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Imagenes<\/mark><\/p>\n\n\n\n

<\/p>\n\n\n\n

\"\"
Figure 1:<\/strong> SDS-PAGE of GPBAR1, purified via Rho1D4 affinity chromatography. L: Lysate: FT: flow-through, W: Wash, E1-E3: elution fractions with purified GPCR.<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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Figure 2:<\/strong> Surface plasmon resonance measurement of GPBAR-1 \/ Taurolithocholic acid sulfate salt interaction. Using a 1:1 binding model, the Kd was determined to be 1.1x10e-8 M<\/sup>.<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Descargo de responsabilidad<\/mark>:<\/strong> Nuestros productos est\u00e1n destinados para investigacion en aplicaciones de biolog\u00eda molecular. Estos productos no est\u00e1n destinados al diagn\u00f3stico, prevenci\u00f3n o tratamiento de una enfermedad.<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Proteina de Cube Biotech<\/p>\n","protected":false},"featured_media":9590,"template":"","al_product-cat":[39,34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9587"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9587\/revisions"}],"predecessor-version":[{"id":9597,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9587\/revisions\/9597"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9590"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9587"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9587"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}