{"id":9581,"date":"2023-03-27T12:50:13","date_gmt":"2023-03-27T18:50:13","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9581"},"modified":"2023-03-27T14:28:55","modified_gmt":"2023-03-27T20:28:55","slug":"proteina-mc4r","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/proteina-mc4r\/","title":{"rendered":"Proteina MC4R\u00a0"},"content":{"rendered":"\n

Las prote\u00ednas ProteinsPure & Active Membrane son dif\u00edciles de obtener y mantener. Por lo tanto, ofrecemos una selecci\u00f3n de prote\u00ednas de membrana puras y activas para su comodidad. MC4R (receptor 4 de melanocortina) de H. sapiens se expres\u00f3 en SF9 (baculovirus) y se purific\u00f3 con nuestra resina de agarosa PureCube Rho1D4<\/em> de alta calidad.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
CU-28401<\/td>Proteina MC4R<\/td>10 ug<\/td><\/tr>
CU-28403<\/td>Proteina MC4R<\/td>50 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Datasheet MC4R<\/a><\/p>\n<\/div>\n\n\n\n

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Caracteristicas<\/mark><\/p>\n\n\n\n

Alternative names<\/td>MC4-R, Melanocortin receptor 4<\/td><\/tr>
UniProt Number<\/td>QP32245<\/td><\/tr>
Protein class<\/td>GPCR (G-protein coupled receptor)<\/td><\/tr>
Organism<\/td>Human (Homo sapiens)<\/td><\/tr>
Sequence, explained<\/td>Full-length, wildtype sequence GSSG spacer and C-terminal Rho1D4-tag (TETSQVAPA)<\/td><\/tr>
Sequence, One-Letter Code<\/td>MKTIIA LSYIFCLVFA MVNSTHRGMH TSLHLWNRSS
YRLHSNASES LGKGYSDGGC YEQLFVSPEV FVTLGVISLL
ENILVIVAI AKNKNLHSPM YFFICSLAVA DMLVSVSNGS
ETIVITLLNS TDTDAQSFTV NIDNVIDSV ICSSLLASIC
SLLSIAVDRY FTIFYALQYH NIMTVKRVGI IISCIWAACT
VSGILFIIY SDSSAVIICL ITMFFTMLAL MASLYVHMFL
MARLHIKRIA VLPGTGAIRQ GANMKGAIT LTILIGVFVV
CWAPFFLHLI FYISCPQNPY CVCFMSHFNL YLILIMCNSI
IDPLIYALR SQELRKTFKE IICCYPLGGL CDLSSRYENL
YFQGGS SGTETSQVAPA<\/td><\/tr>
Affinity tags<\/td>C-terminal Rho1D4-tag<\/td><\/tr>
Expression Host<\/td>T. Ni<\/em> insect cells (baculovirus expression system)<\/td><\/tr>
Size (excluding additional elements)<\/td>339 kDA<\/td><\/tr>
Buffer composition<\/td>20 mM Hepes, pH 7.5; 100 mM NaCl; 2 mM EDTA; 0,1 % DM<\/td><\/tr>
Purity<\/td>>98%, determined via SDS-PAGE<\/td><\/tr>
Homogeneity<\/td>Size exclusion chromatography<\/td><\/tr>
Purified via<\/td>PureCube Rho1d4 agarose resin<\/a><\/td><\/tr>
Function<\/td>Receptor specific to the heptapeptide core common to adrenocorticotropic hormone and alpha-, beta-, and gamma-MSH. Plays a central role in energy homeostasis and somatic growth. This receptor is mediated by G proteins that stimulate adenylate cyclase (cAMP)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n<\/mark>
Expression system:<\/strong> T.ni (Baculovirus)
Purification:<\/strong> PureCube Rho1D4 Agarose and size exclusion chromatography
Buffer:<\/strong> 20 mM Hepes, pH 7.5; 100 mM NaCl; 2 mM EDTA; 0,1% decylmaltoside
Form<\/strong>: Liquid<\/p>\n\n\n\n

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Control de Calidad<\/mark>
Pureza: (SDS-PAGE):<\/strong> >95% de acuerdo a lo determinado por SDS-PAGE, ver Fig. 1
Homogeneidad: <\/strong> Tama\u00f1o exclusi\u00f3n a cromatograf\u00eda
Actividad:<\/strong> No determinado<\/p>\n\n\n\n

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Aplicaciones<\/mark>
\u2022 SDS-PAGE
\u2022 Western Blot
\u2022 Protein Crystallization
\u2022 Biochemical and biophysical analyses<\/p>\n\n\n\n

\u2022 Ligand binding assays (e.g. SPR)<\/p>\n\n\n\n

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Envio y Almacenamiento<\/mark><\/p>\n\n\n\n

Este producto se envia en hielo seco y se almacena a -80\u00b0C. Evitar los ciclos de congelaci\u00f3n y descongelaci\u00f3n. <\/p>\n\n\n\n

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Imagenes<\/mark><\/p>\n\n\n\n

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Figure 1:<\/strong> Western Blot and SDS-PAGE of MC4R<\/figcaption><\/figure>\n\n\n\n
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Figure 2:<\/strong> MC4R was purified via Rho1d4-tag affinity chromatography<\/figcaption><\/figure>\n\n\n\n
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Descargo de responsabilidad<\/mark>:<\/strong> Nuestros productos est\u00e1n destinados para investigacion en aplicaciones de biolog\u00eda molecular. Estos productos no est\u00e1n destinados al diagn\u00f3stico, prevenci\u00f3n o tratamiento de una enfermedad.<\/p>\n\n\n\n

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Proteina de Cube Biotech<\/p>\n","protected":false},"featured_media":9584,"template":"","al_product-cat":[39,34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9581"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9581\/revisions"}],"predecessor-version":[{"id":9598,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9581\/revisions\/9598"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9584"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9581"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9581"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}