{"id":9574,"date":"2023-03-27T12:15:30","date_gmt":"2023-03-27T18:15:30","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&#038;p=9574"},"modified":"2023-03-27T14:30:53","modified_gmt":"2023-03-27T20:30:53","slug":"r","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/r\/","title":{"rendered":"Proteina eGFP (His-tag)\u00a0"},"content":{"rendered":"\n<p>El descubrimiento de la Prote\u00edna Verde Fluorescente (GFP) es una de las prote\u00ednas m\u00e1s influyentes en la biolog\u00eda moderna. Enhanced GFP (eGFP) es una versi\u00f3n modificada de GFP que exhibe una fluorescencia a\u00fan m\u00e1s fuerte para una detecci\u00f3n m\u00e1s f\u00e1cil. La eGFP purificada puede encontrar m\u00faltiples usos en la investigaci\u00f3n cient\u00edfica. A menudo como controles positivos para purificaciones de prote\u00ednas o transferencias Western. Por lo tanto, ofrecemos eGFP etiquetado con las etiquetas de afinidad m\u00e1s utilizadas para estos fines.<\/p>\n\n\n\n<p>Ofrecemos eGFP con las siguientes combinaciones de etiquetas de afinidad<\/p>\n\n\n\n<ul><li>N-Terminal His-tag<\/li><li>N-Terminal GST-tag and C-Terminal Rho1D4-tag<\/li><li>N-Terminal GST-tag and C-Terminal DYKDDDDK-tag<\/li><\/ul>\n\n\n\n<div style=\"height:28px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table\" style=\"font-size:18px\"><table class=\"has-black-color has-text-color has-background\" style=\"background:linear-gradient(0deg,rgb(238,238,238) 0%,rgb(255,255,255) 28%,rgb(249,249,249) 72%,rgb(169,184,195) 100%)\"><thead><tr><th>Catalogo<\/th><th>Producto<\/th><th>Presentaci\u00f3n<\/th><\/tr><\/thead><tbody><tr><td>CU-29901<\/td><td>His-eGFP<\/td><td>50 ug<\/td><\/tr><tr><td>CU-29903<\/td><td>His-eGFP<\/td><td>250 ug<\/td><\/tr><tr><td>CU-29911<\/td><td>GST-eGFP-Rho1D4<\/td><td>50 ug<\/td><\/tr><tr><td>CU-29913<\/td><td>GST-eGFP-Rho1D4<\/td><td>250 ug<\/td><\/tr><tr><td>CU-29921<\/td><td>GST-eGFP-DYKDDDDK-tag<\/td><td>50 ug<\/td><\/tr><tr><td>CU-29923<\/td><td>GST-eGFP-DYKDDDDK-tag<\/td><td>250 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:18px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-container-1 is-nowrap wp-block-group\">\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/cube-biotech.com\/media\/g0\/e8\/55\/1668421207\/29901%20eGFP%20His-tag.pdf\" target=\"_blank\" rel=\"noopener\" title=\"\">Datasheet eGFP (His-tag)<\/a><\/p>\n<\/div>\n\n\n\n<div class=\"wp-container-2 is-nowrap wp-block-group\">\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/cube-biotech.com\/media\/cf\/67\/22\/1668421207\/29911%20eGFP%20%20GST-%20und%20Rho-tag.pdf\" target=\"_blank\" rel=\"noopener\" title=\"\">Datasheet eGFP Rho1D4<\/a><\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><\/p>\n<\/div>\n\n\n\n<div class=\"wp-container-4 wp-block-group\"><div class=\"wp-block-group__inner-container\">\n<div class=\"wp-container-3 is-nowrap wp-block-group\">\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/cube-biotech.com\/media\/e8\/4a\/ec\/1668421207\/29921%20eGFP%20GST%20and%20FLAG%20tag.pdf\" target=\"_blank\" rel=\"noopener\" title=\"\">Datasheet eGFP DYKDDDDK-tag<\/a><\/p>\n<\/div>\n<\/div><\/div>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Envio y Almacenamiento<\/mark><\/p>\n\n\n\n<p style=\"font-size:16px\">Estos productos se envian a temperatura ambiente.  Se almacena a -20\u00b0C. Evitar exposici\u00f3n a la luz. Mantener en ambiente  obscuro. <\/p>\n\n\n\n<div style=\"height:85px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Caracteristicas<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-white-background-color has-background\"><tbody><tr><th><\/th><th><strong>EGFP (HIS-TAG)<\/strong><\/th><\/tr><tr><td>Protein class<\/td><td>Beta-barrel; Autofluorescent Protein<\/td><\/tr><tr><td>Sequence &#8211; Sections named from N-Ter to C-Ter<\/td><td>His-tag, eGFP<\/td><\/tr><tr><td>Sequence &#8211; Amino Acids (1-Letter-Code)<\/td><td><em>N-terminus-<\/em><br>MKHHHHHHHMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL<br>KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIF<br>FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNGHNVYITA<br>DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSK<br>DPNEKRDHMVLLEFVTAAGITHGMDELYK<em>-C-terminus<\/em><\/td><\/tr><tr><td>Affinity tags<\/td><td>His-tag (N-Terminus)<\/td><\/tr><tr><td>Size (kDA)<\/td><td>26 kDA<\/td><\/tr><tr><td>Size (Amino Acid count)<\/td><td>249<\/td><\/tr><tr><td>Excitation maximum<\/td><td>488 nm<\/td><\/tr><tr><td>Fluorescence maximum<\/td><td>509 nm<\/td><\/tr><tr><td>Purity<\/td><td>&gt;98% as determined by SDS-PAGE<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><th>Caracteristicas<\/th><th>EGFP (GST- AND RHO1D4-TAG)<\/th><\/tr><tr><td>Protein class<\/td><td>Beta-barrel; Autofluorescent Protein<\/td><\/tr><tr><td>Sequence &#8211; Sections named from N-Ter to C-Tery<\/td><td>GST-tag, Thrombin cleavage site, HRV3C (PreScission) cleavage site, eGFP, Spacer, Rho1D4-tag<\/td><\/tr><tr><td>Sequence &#8211; Amino Acids (1-Letter-Code)<\/td><td><em>N-terminus-<\/em><br>MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNL<br>PYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIA<br>YSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM<br>DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSD<br>LVPRGSLEVLFQGPMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY<br>GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQ<br>ERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNGHN<br>VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ<br>SALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSSGTETSQVAPA<em>-C-terminus<\/em><\/td><\/tr><tr><td>Affinity tags<\/td><td>GST-tag (N-Terminus), Rho1D4-tag (C-terminus)<\/td><\/tr><tr><td>Size (kDA)<\/td><td>55.4 kDA<\/td><\/tr><tr><td>Size (Amino Acid count)<\/td><td>488<\/td><\/tr><tr><td>Excitation maximum<\/td><td>488 nm<\/td><\/tr><tr><td>Fluorescence maximum<\/td><td>509 nm<\/td><\/tr><tr><td>Purity<\/td><td>&gt;98% as determined by SDS-PAGE<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><th>Caracteristicas<\/th><th>EGFP (GST- AND DYKDDDDK-TAG)<\/th><\/tr><tr><td>Protein class<\/td><td>Beta-barrel; Autofluorescent Protein<\/td><\/tr><tr><td>Sequence &#8211; Sections named from N-Ter to C-Tery<\/td><td>GST-tag, Thrombin cleavage site, HRV3C (PreScission) cleavage site, eGFP Spacer, DYKDDDDK-tag<\/td><\/tr><tr><td>Sequence &#8211; Amino Acids (1-Letter-Code)<\/td><td><em>N-terminus-<\/em><br>MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNL<br>PYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIA<br>YSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYM<br>DPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSD<br>LVPRGSLEVLFQGPMRSGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATY<br>GKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQ<br>ERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNGHN<br>VYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQ<br>SALSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGSSGDYKDDDDK<em>-C-terminus<\/em><\/td><\/tr><tr><td>Affinity tags<\/td><td>GST-tag (N-Terminus), Rho1D4-tag (C-terminus)<\/td><\/tr><tr><td>Size (kDA)<\/td><td>55.5 kDA<\/td><\/tr><tr><td>Size (Amino Acid count)<\/td><td>486<\/td><\/tr><tr><td>Excitation maximum<\/td><td>488 nm<\/td><\/tr><tr><td>Fluorescence maximum<\/td><td>509 nm<\/td><\/tr><tr><td>Purity<\/td><td>&gt;98% as determined by SDS-PAGE<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:87px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Aplicaciones<\/mark><br>\u2022 SDS-PAGE<br>\u2022 Western Blot<br>\u2022 Protein Crystallization<br>\u2022 Biochemical and biophysical analyses<\/p>\n\n\n\n<div style=\"height:78px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Resultado de Laboratorio<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-200.png\" alt=\"\" class=\"wp-image-9567\" width=\"330\" height=\"324\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-200.png 256w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-200-163x160.png 163w\" sizes=\"(max-width: 330px) 100vw, 330px\" \/><figcaption><strong>Figure 1:<\/strong>&nbsp;Size and purity of three eGFP variants assessed by SDS-PAGE.<\/figcaption><\/figure>\n\n\n\n<div style=\"height:72px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><strong><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-red-color\">Descargo de responsabilidad:<\/mark><\/strong> Nuestros productos est\u00e1n destinados para uso en aplicaciones de biolog\u00eda molecular. Estos productos no est\u00e1n destinados al diagn\u00f3stico, prevenci\u00f3n o tratamiento de una enfermedad.<\/p>\n\n\n\n<div style=\"height:88px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Proteina de Cube Biotech<\/p>\n","protected":false},"featured_media":9572,"template":"","al_product-cat":[39,34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9574"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":4,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9574\/revisions"}],"predecessor-version":[{"id":9601,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9574\/revisions\/9601"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9572"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9574"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9574"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}