{"id":9557,"date":"2023-03-26T20:48:27","date_gmt":"2023-03-27T02:48:27","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9557"},"modified":"2023-03-27T22:10:38","modified_gmt":"2023-03-28T04:10:38","slug":"sensory-rhodopsin-2-np","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/sensory-rhodopsin-2-np\/","title":{"rendered":"Sensory Rhodopsin-2 (Np)"},"content":{"rendered":"\n

La proteina Sensory Rhodopsin-2 (Np) es una prote\u00edna de 7 transmembrana que act\u00faa como receptor de fototaxis en las arqueobacterias. Debido a su ligando coloreado unido covalentemente, SR-2 es ideal para usar como prote\u00edna de membrana de control, por ejemplo, para ensayos de cristalizaci\u00f3n y experimentos bioqu\u00edmicos\/biof\u00edsicos. Proporcionamos SR-2 de alta pureza, purificado a partir de E. coli como una prote\u00edna etiquetada con His.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
CU-28931<\/td>Proteina Sensory Rhodopsin-2 (Np)<\/td>100 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Datasheet<\/a><\/p>\n<\/div>\n\n\n\n

Envio y Almacenamiento<\/mark><\/p>\n\n\n\n

El producto se envia en hielo seco. Se almacena a -80\u00b0C. Evitar exposici\u00f3n a la luz. Mantener en ambiente obscuro. <\/p>\n\n\n\n

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Control de calidad <\/mark>
Pureza: <\/strong> >98% as determined by SDS-PAGE, see Figure 1
Actividad:<\/strong> Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm \/ 500 nm = 2.8, see Figure 2<\/p>\n\n\n\n

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Caracteristicas<\/mark><\/p>\n\n\n\n

Alternative names<\/td>Sensory rhodopsin-2 (SR-2, NpSRII)<\/td><\/tr>
UniProt Number<\/td>P42196<\/a><\/td><\/tr>
Protein class<\/td>7-transmembrane protein<\/td><\/tr>
Organism<\/td>Natronomonas pharaonis (Natronobacterium pharaonis)<\/em><\/td><\/tr>
Sequence, explained<\/td>wild-type sequence, full-length, 7x His-tag<\/td><\/tr>
Sequence, One-Letter Code<\/td>MVGLTTLFWL GAIGMLVGTL AFAWAGRDAG SGERRYYVTL VGISGIAAVA YVVMALGVGW VPVAERTVFA PRYIDWILTT PLIVYFLGLL AGLDSREFGI VITLNTVVML AGFAGAMVPG IERYALFGMG AVAFLGLVYY LVGPMTESAS QRSSGIKSLY VRLRNLTVIL WAIYPFIWLL GPPGVALLTP TVDVALIVYL DLVTKVGFGF IALDAAATLR AEHGESLAGV DTDAPAVADH HHHHHH<\/td><\/tr>
Affinity tags<\/td>His-tag (C-terminus)<\/td><\/tr>
Expression Host<\/td>E.coli<\/em><\/td><\/tr>
Size (excluding additional elements)<\/td>246 amino acids; 26,315 Da<\/td><\/tr>
Buffer composition<\/td>4 M NaCl, 300 mM NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)<\/td><\/tr>
Purity<\/td>>98%, determined via SDS-PAGE (Figure 1)<\/td><\/tr>
Purified via<\/td>PureCube Ni-NTA Agarose<\/a><\/td><\/tr>
Absorbance<\/td>Extinction coefficient at 500 nm: 45,500 M-1<\/sup>\u00a0cm-1<\/sup>..
Extinction coefficient at 590 nm: 54,000 M-1<\/sup>\u00a0cm-1<\/sup>.<\/td><\/tr>
Activity<\/td>Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm \/ 500 nm = 2.8<\/td><\/tr>
Function<\/td>Photoreceptor protein undergoing a photocycle, Retinal protein, phototaxis receptor<\/td><\/tr>
Literature references<\/td>Gordeliy, Valentin I., et al. “Molecular basis of transmembrane signalling by sensory rhodopsin II\u2013transducer complex.” Nature 419.6906 (2002): 484.<\/a>
Orekhov, Philipp, et al. “Sensory rhodopsin I and sensory rhodopsin II form trimers of dimers in complex with their cognate transducers.” Photochemistry and photobiology 93.3 (2017): 796-804.<\/a><\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n<\/mark>
Expression system: <\/strong> E. coli
Purification:<\/strong> PureCube Ni-NTA Agarose
Buffer:<\/strong> 300 mM NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Concentration:<\/strong> 8.95 mg\/mL
Volume:<\/strong> 12 \u00b5L per 100 \u00b5g aliquot<\/p>\n\n\n\n

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Aplicaciones<\/mark>
\u2022 SDS-PAGE
\u2022 Western Blot
\u2022 Protein Crystallization
\u2022 Biochemical and biophysical analyses<\/p>\n\n\n\n

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Resultado de Laboratorio<\/mark><\/p>\n\n\n\n

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Figure 1:<\/strong> SDS-PAGE of sensory rhodopsin-2 from N. pharaonis.<\/em><\/figcaption><\/figure>\n\n\n\n
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Figure 2:<\/strong> Absorbance spectrum of sensory rhodopsin-2 from N. pharaonis.<\/em><\/figcaption><\/figure>\n\n\n\n
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Descargo de responsabilidad:<\/strong> <\/mark>Nuestros productos est\u00e1n destinados a aplicaciones de investigacion en biolog\u00eda molecular. Estos productos no est\u00e1n destinados al diagn\u00f3stico, prevenci\u00f3n o tratamiento de una enfermedad.<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Proteina de Cube Biotech<\/p>\n","protected":false},"featured_media":9560,"template":"","al_product-cat":[39,34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9557"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9557\/revisions"}],"predecessor-version":[{"id":9628,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9557\/revisions\/9628"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9560"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9557"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9557"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}