{"id":9542,"date":"2023-03-26T19:42:56","date_gmt":"2023-03-27T01:42:56","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9542"},"modified":"2023-03-26T21:58:58","modified_gmt":"2023-03-27T03:58:58","slug":"sensory-rhodopsin-1-hs","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/sensory-rhodopsin-1-hs\/","title":{"rendered":"Sensory Rhodopsin-1 (Hs)"},"content":{"rendered":"\n

La rodopsina-1 sensorial (SR-1) es una prote\u00edna de 7 transmembrana que act\u00faa como un receptor de fototaxis en las arqueobacterias. Debido a su ligando coloreado unido covalentemente, SR-1 es ideal para usar como prote\u00edna de membrana de control, por ejemplo, para ensayos de cristalizaci\u00f3n y experimentos bioqu\u00edmicos\/biof\u00edsicos. Proporcionamos SR-1 de alta pureza, purificado a partir de E. coli como una prote\u00edna etiquetada con His.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
CU-28911<\/td>Proteina Sensory Rhodopsin-1 (Hs)<\/td>100 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Datasheet<\/a><\/p>\n<\/div>\n\n\n\n

Envio y Almacenamiento<\/mark><\/p>\n\n\n\n

El producto se envia en hielo seco. Se almacena a -80\u00b0C. Evitar exposici\u00f3n a la luz. Mantener en ambiente obscuro. <\/p>\n\n\n\n

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Control de calidad <\/mark>
Pureza: <\/strong> >98% as determined by SDS-PAGE, see Figure 1
Actividad:<\/strong> Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm \/ 590 nm = 1.7, see Figure 2<\/p>\n\n\n\n

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Caracteristicas<\/mark><\/p>\n\n\n\n

Alternative names<\/td>Sensory rhodopsin-1 (SR-1, HsSRI)<\/td><\/tr>
UniProt Number<\/td>P0DMH8<\/a><\/td><\/tr>
Protein class<\/td>7-transmembrane protein<\/td><\/tr>
Organism<\/td>Halobacterium salinarum <\/em>S9<\/td><\/tr>
Sequence, explained<\/td>wild-type sequence 1-234 aa (lacking 15 aa at the C-terminus), 10x His-tag<\/td><\/tr>
Sequence, One-Letter Code<\/td>MDAVATAYLG GAVALIVGVA FVWLLYRSLD GSPHQSALAP LAIIPVFAGL SYVGMAYDIG TVIVNGNQIV GLRYIDWLVT TPILVGYVGY AAGASRRSII GVMVADALMI AVGAGAVVTD GTLKWALFGV SSIFHLSLFA YLYVIFPRVV PDVPEQIGLF NLLKNHIGLL WLAYPLVWLF GPAGIGEATA AGVALTYVFL DVLAKVPYVY FFYARRRVFM HSESHHHHHH HHHH<\/td><\/tr>
Affinity tags<\/td>His-tag (C-terminus)<\/td><\/tr>
Expression Host<\/td>E.coli<\/em><\/td><\/tr>
Size (excluding additional elements)<\/td>234 amino acids; 25,423 Da<\/td><\/tr>
Buffer composition<\/td>4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)<\/td><\/tr>
Purity<\/td>>98%, determined via SDS-PAGE (Figure 1)<\/td><\/tr>
Purified via<\/td>PureCube Ni-NTA Agarose<\/a><\/td><\/tr>
Absorbance<\/td>Extinction coefficient at 560 nm: 63,000 M-1<\/sup> cm-1<\/sup>.<\/td><\/tr>
Activity<\/td>Binding of ligand all-trans retinal, covalently bound to a lysine residue. Evaluation of UV-VIS spectrum: absorbance ratio 280 nm \/ 590 nm = 1.7<\/td><\/tr>
Function<\/td>Photoreceptor protein undergoing a photocycle, Retinal protein, phototaxis receptor<\/td><\/tr>
Literature references<\/td>Radu, Ionela, et al. “Signal relay from sensory rhodopsin I to the cognate transducer HtrI: Assessing the critical change in hydrogen-bonding between Tyr-210 and Asn-53.” Biophysical chemistry 150.1-3 (2010): 23-28.<\/a>Schmies, Georg, Igor Chizhov, and Martin Engelhard. “Functional expression of His\u2010tagged sensory rhodopsin I in Escherichia coli.” FEBS letters 466.1 (2000): 67-69.<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n<\/mark>
Expression system: <\/strong> E. coli
Purification:<\/strong> PureCube Ni-NTA Agarose
Buffer:<\/strong> 4 M NaCl, 50 mM MES pH 6.0, 0.03% dodecyl maltoside (DDM)
Concentration:<\/strong> 2.7 mg\/mL
Volume:<\/strong> 40 \u00b5L per 100 \u00b5g aliquot<\/p>\n\n\n\n

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Aplicaciones<\/mark>
\u2022 SDS-PAGE
\u2022 Western Blot
\u2022 Protein Crystallization
\u2022 Biochemical and biophysical analyses<\/p>\n\n\n\n

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Resultado de Laboratorio<\/mark><\/p>\n\n\n\n

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Figura 1:<\/strong> SDS-PAGE of sensory rhodopsin-1 from H. salinarum.<\/em><\/figcaption><\/figure>\n\n\n\n
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Figura 2:<\/strong> Absorbance spectrum of sensory rhodopsin-1 from H. salinarum.<\/em><\/figcaption><\/figure>\n\n\n\n
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Descargo de responsabilidad:<\/strong> Nuestros productos est\u00e1n destinados a aplicaciones de biolog\u00eda molecular. Estos productos no est\u00e1n destinados al diagn\u00f3stico, prevenci\u00f3n o tratamiento de una enfermedad.<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Proteina de Cube Biotech<\/p>\n","protected":false},"featured_media":9545,"template":"","al_product-cat":[39,34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9542"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":4,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9542\/revisions"}],"predecessor-version":[{"id":9571,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9542\/revisions\/9571"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9545"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9542"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9542"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}