{"id":9536,"date":"2023-03-26T19:11:30","date_gmt":"2023-03-27T01:11:30","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9536"},"modified":"2023-03-26T19:41:21","modified_gmt":"2023-03-27T01:41:21","slug":"proteina-vgt","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/proteina-vgt\/","title":{"rendered":"Prote\u00edna VGT"},"content":{"rendered":"\n

Las prote\u00ednas ProteinsPure & Active son dif\u00edciles de obtener y mantener. Por lo tanto, ofrecemos una selecci\u00f3n de prote\u00ednas de membrana puras y activas para su comodidad. El VGT (transportador de glutamato vesicular 2) de H. sapiens se expres\u00f3 en c\u00e9lulas SF9 transvectadas con Baculovirus y se purific\u00f3 con nuestra resina de agarosa PureCube Rho1d4<\/em> de alta calidad.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
CU-28501<\/td>Proteina VGT<\/td>10 ug<\/td><\/tr>
CU-28503<\/td>Proteina VGT<\/td>50 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

Datasheet<\/a><\/p>\n<\/div>\n\n\n\n

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Forma:<\/strong> En soluci\u00f3n (20 mM Hepes, 100 mM NaCl, 0,05% DDM, 5% Glicerol pH 7,2)
Etiqueta:<\/strong> C-terminal Rho1D4-tag<\/p>\n\n\n\n

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Envio y Almacenamiento<\/mark><\/p>\n\n\n\n

El producto se envia a temperatura <-15\u00b0C. Se almacena a -80\u00b0C.<\/p>\n\n\n\n

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Caracteristicas<\/mark><\/p>\n\n\n\n

Nombres Alternativos<\/td>VGluT2, Differentiation-associated BNPI, Solute carrier family 17 member 6<\/td><\/tr>
Numero UniProt <\/td>Q9P2U8<\/td><\/tr>
Clase <\/td>Organic anion transporter<\/td><\/tr>
Organismo<\/td>Humano (Homo sapiens<\/em>)<\/td><\/tr>
Secuencia explicada<\/td>Full-length, wildtype sequence GSSG spacer and C-terminal Rho1D4-tag (TETSQVAPA)<\/td><\/tr>
Secuencia, Codigo One-Letter <\/td>MESVKQRILA PGKEGLKNFA GKSLGQIYRV LEKKQDTGET IELTEDGKPL EVPERKAPLC DCTCFGLPRR YIIAIMSGLG FCISFGIRCN LGVAIVDMVN NSTIHRGGKV IKEKAKFNWD PETVGMIHGS FFWGYIITQI PGGYIASRLA ANRVFGAAIL LTSTLNMLIP SAARVHYGCV IFVRILQGLV EGVTYPACHG IWSKWAPPLE RSRLATTSFC GSYAGAVIAM PLAGILVQYT GWSSVFYVYG SFGMVWYMFW LLVSYESPAK HPTITDEERR YIEESIGESA NLLGAMEKFK TPWRKFFTSM PVYAIIVANF CRSWTFYLLL ISQPAYFEEV FGFEISKVGM LSAVPHLVMT IIVPIGGQIA DFLRSKQILS TTTVRKIMNC GGFGMEATLL LVVGYSHTRG VAISFLVLAV GFSGFAISGF NVNHLDIAPR YASILMGISN GVGTLSGMVC PIIVGAMTKN KSREEWQYVF LIAALVHYGG VIFYAIFASG EKQPWADPEE TSEEKCGFIH EDELDEETGD ITQNYINYGT TKSYGATTQA NGGWPSGWEK KEEFVQGEVQ DSHSYKDRVD YSGS SGTETSQVAPA<\/td><\/tr>
Affinity tags<\/td>C-terminal Rho1D4-tag<\/td><\/tr>
Expression Host<\/td>T. Ni<\/em> insect cells (baculovirus expression system)<\/td><\/tr>
Size (excluding additional elements)<\/td>65 kDA<\/td><\/tr>
Composicion Buffer <\/td>20 mM Hepes, 10 % glycerol, 0,05% DDM, pH 7,2<\/td><\/tr>
Pureza<\/td>>95%, determined via SDS-PAGE<\/td><\/tr>
Homogeneity<\/td>Size exclusion chromatography<\/td><\/tr>
Purified via<\/td>PureCube Rho1d4 agarose resin<\/a><\/td><\/tr>
Function<\/td>Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Resultado de Laboratorio<\/mark><\/p>\n\n\n\n

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Figura 1:<\/strong>\u00a0Western Blot and SDS-PAGE of VGT<\/figcaption><\/figure>\n\n\n\n
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Figura 2:<\/strong>\u00a0VGT was purified via Rho1d4-tag affinity chromatography<\/figcaption><\/figure>\n\n\n\n
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FAQ<\/mark><\/p>\n\n\n\n

\u2022 \u00bfQu\u00e9 puedo hacer con las perlas de Zr-NTA?<\/strong><\/p>\n\n\n\n

R: Se pueden utilizar como matriz para unir y enriquecer as\u00ed los fosfop\u00e9ptidos. <\/p>\n\n\n\n

\u2022 \u00bfC\u00f3mo funciona el proceso de enriquecimiento de fosfop\u00e9ptidos?<\/strong><\/p>\n\n\n\n

R: Utilice el protocolo disponible como referencia. <\/p>\n\n\n\n

\u2022 \u00bfPuedo enriquecer todos los fosfop\u00e9ptidos usando Zr-NTA?<\/strong><\/p>\n\n\n\n

R: No, esto no es posible. No todos los fosfop\u00e9ptidos se unen a las perlas de Zr-NTA. Como se muestra en la figura 2, se deben usar m\u00faltiples tipos de perlas para cubrir todos los fosfop\u00e9ptidos de una c\u00e9lula. Por eso, tambi\u00e9n ofrecemos perlas de Ti-, Zr- y Al-NTA.<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Cube Biotech<\/p>\n","protected":false},"featured_media":9539,"template":"","al_product-cat":[39,34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9536"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9536\/revisions"}],"predecessor-version":[{"id":9541,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9536\/revisions\/9541"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9539"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9536"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9536"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}