{"id":9215,"date":"2023-03-22T18:21:57","date_gmt":"2023-03-23T00:21:57","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9215"},"modified":"2023-03-22T18:47:37","modified_gmt":"2023-03-23T00:47:37","slug":"indicador-bcecf-am","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/indicador-bcecf-am\/","title":{"rendered":"Indicador BCECF AM"},"content":{"rendered":"\n

BCECF AM es el indicador de pH intracelular verde fluorescente m\u00e1s popular. Tiene un pKa de ~7 y exhibe propiedades de excitaci\u00f3n dual dependientes del pH (Ex\/Em 430nm\/535nm y 490nm\/535nm) para el an\u00e1lisis radiom\u00e9trico. La ratiometr\u00eda es \u00f3ptima para aplicaciones de im\u00e1genes y reduce los efectos del fotoblanqueo, la carga heterog\u00e9nea de colorantes y la morfolog\u00eda celular variable. <\/p>\n\n\n\n

Para aplicaciones HTS, BCECF tambi\u00e9n se puede usar en modo no radiom\u00e9trico utilizando configuraciones est\u00e1ndar de excitaci\u00f3n y emisi\u00f3n de fluoresce\u00edna.<\/p>\n\n\n\n

Sin\u00f3nimo: 2\u2032,7\u2032-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein \u2013 AM<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
ON-4011B<\/td>Indicador BCECF AM<\/td>1 mg<\/td><\/tr>
ON-4011E<\/td>Indicador BCECF AM<\/td>20 x 50 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

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Especificaciones<\/a><\/p>\n<\/div>\n\n\n\n

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Especificaciones<\/strong><\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>430 nm \"\" 490 nm <\/strong>\"\" <\/td><\/tr>
Emisi\u00f3n<\/td>535 nm  <\/strong>\"\" <\/td><\/tr>
Solubilidad<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Optimo para aplicaciones de im\u00e1genes radiom\u00e9tricas. Reduce los efectos del fotoblanqueo, la carga heterog\u00e9nea de colorantes y la morfolog\u00eda celular variable. Tambi\u00e9n es compatible con HTS usando an\u00e1lisis no radiom\u00e9trico. <\/p>\n\n\n\n

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Garantia ON Assurance. La calidad de este producto esta garantizado. Si no est\u00e1 satisfecho con su pedido, trabajaremos para resolver de inmediato su inquietud de acuerdo con nuestros t\u00e9rminos y condiciones de ION Assurance.<\/p>\n\n\n\n

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Referencias<\/strong><\/mark><\/p>\n\n\n\n

Clementi EA, Marks LR, Roche-H\u00e5kansson H, H\u00e5kansson AP. Monitoring changes in membrane polarity, membrane integrity, and intracellular ion concentrations in Streptococcus pneumoniae using fluorescent dyes. J Vis Exp. 2014 Feb 17;(84):e51008. doi: 10.3791\/51008<\/a><\/p>\n\n\n\n

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Oernbo, Eva K et al. \u201cMembrane transporters control cerebrospinal fluid formation independently of conventional osmosis to modulate intracranial pressure.\u201d Fluids and barriers of the CNS<\/em> vol. 19,1 65. 29 Aug. 2022<\/a><\/p>\n\n\n\n

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Rink TJ, Tsien RY, Pozzan T. Cytoplasmic pH and free Mg2+ in lymphocytes. J Cell Biol. 1 October 1982; 95 (1): 189\u2013196.<\/a> doi: https:\/\/doi.org\/10.1083\/jcb.95.1.189<\/a><\/p>\n\n\n\n

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Indicador de pH radiom\u00e9trico, verde fluorescente, permeable a la membrana.<\/p>\n","protected":false},"featured_media":9217,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9215"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9215\/revisions"}],"predecessor-version":[{"id":9220,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9215\/revisions\/9220"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9217"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9215"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}