{"id":9116,"date":"2023-03-20T20:56:38","date_gmt":"2023-03-21T02:56:38","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=9116"},"modified":"2023-03-20T21:38:11","modified_gmt":"2023-03-21T03:38:11","slug":"indicador-fluo-3-am","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/indicador-fluo-3-am\/","title":{"rendered":"Indicador Fluo-3 AM"},"content":{"rendered":"\n

Fluo-3 AM es un indicador de calcio intracelular fluorescente verde con un amplio rango din\u00e1mico en respuesta al Ca\u00b2\u207a. Mejor que Fluo-4 para algunas aplicaciones. <\/p>\n\n\n\n

Fluo-3 es un indicador de calcio con propiedades espectrales ligeramente desplazadas hacia el rojo en comparaci\u00f3n con Fluo-4. Ofrece un amplio rango din\u00e1mico que puede ser m\u00e1s adecuado para aplicaciones de im\u00e1genes de alto contraste. <\/p>\n\n\n\n

Para algunos tipos de c\u00e9lulas, se prefiere Fluo-3 contra el indicador Fluo-4.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
ON-1031B<\/td>Indicador Fluo-3 AM <\/td>1 mg<\/td><\/tr>
ON-1031E<\/td>Indicador Fluo-3 AM <\/td>20 x 50 ug<\/td><\/tr>
ON-1031<\/td>Indicador Fluo-3 AM <\/td>5 x 50 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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SDS<\/a><\/p>\n\n\n\n

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Protocol<\/a> <\/p>\n\n\n\n

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Especificaciones<\/a><\/p>\n<\/div>\n\n\n\n

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Informaci\u00f3n<\/strong><\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>505 nm  <\/strong>\"\"<\/td><\/tr>
Emisi\u00f3n<\/td>525 nm  <\/strong>\"\"<\/td><\/tr>
Kd<\/td>390 nM<\/td><\/tr>
Peso molecular <\/td>1130 g\/mol<\/td><\/tr>
Solubilidad<\/td>DMSO<\/td><\/tr>
Pureza<\/td>>95%<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Productos Relacionados<\/strong><\/mark><\/p>\n\n\n\n

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Fluo-2 AM<\/figcaption><\/figure>\n<\/div>\n\n\n\n
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Fura-2 LR AM<\/figcaption><\/figure>\n<\/div>\n\n\n\n
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Fluo Gold AM<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n
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Soluci\u00f3n Probenecid <\/figcaption><\/figure>\n<\/div>\n\n\n\n
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Soluci\u00f3n TRS<\/figcaption><\/figure>\n<\/div>\n\n\n\n
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10X Brilliant Calcium assay buffer<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n
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Garantia ON Assurance. La calidad de este producto esta garantizado. Si no est\u00e1 satisfecho con su pedido, trabajaremos para resolver de inmediato su inquietud de acuerdo con nuestros t\u00e9rminos y condiciones de ION Assurance.<\/p>\n\n\n\n

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Referencias<\/strong><\/mark><\/p>\n\n\n\n

Minta A, Kao JP, Tsien RY. Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores. J Biol Chem. 1989 May 15;264(14):8171-8<\/a><\/p>\n\n\n\n

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Hagen BM, Boyman L, Kao JP, Lederer WJ. A comparative assessment of fluo Ca2+ indicators in rat ventricular myocytes. Cell Calcium. 2012 Aug;52(2):170-81. doi: 10.1016\/j.ceca.2012.05.010<\/a><\/p>\n\n\n\n

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Thomas D, Tovey SC, Collins TJ, Bootman MD, Berridge MJ, Lipp P. A comparison of fluorescent Ca2+ indicator properties and their use in measuring elementary and global Ca2+ signals. Cell Calcium. 2000 Oct;28(4):213-23. doi: 10.1054\/ceca.2000.0152<\/a><\/p>\n\n\n\n

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Donnadieu E, Bourguignon LY. Ca2+ signaling in endothelial cells stimulated by bradykinin: Ca2+ measurement in the mitochondria and the cytosol by confocal microscopy. Cell Calcium. 1996 Jul;20(1):53-61. doi: 10.1016\/s0143-4160(96)90050-0<\/a><\/p>\n\n\n\n

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Loughrey CM, MacEachern KE, Cooper J, Smith GL. Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics. Cell Calcium. 2003 Jul;34(1):1-9. doi: 10.1016\/s0143-4160(03)00012-<\/a><\/p>\n\n\n\n

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Vandenberghe PA, Ceuppens JL. Flow cytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with fluo-3, a new fluorescent calcium indicator. J Immunol Methods. 1990 Mar 9;127(2):197-205. doi: 10.1016\/0022-1759(90)90069-8<\/a><\/p>\n\n\n\n

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Schepers E, Glorieux G, Dhondt A, Leybaert . L, Vanholder R. Flow cytometric calcium flux assay: evaluation of cytoplasmic calcium kinetics in whole blood leukocytes. J Immunol Methods. 2009 Aug 31;348(1-2):74-82. doi: 10.1016\/j.jim.2009.07.002<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Fluo-3 AM es un indicador de calcio fluorescente verde. Permeable a la membrana.<\/p>\n","protected":false},"featured_media":9119,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9116"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9116\/revisions"}],"predecessor-version":[{"id":9122,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9116\/revisions\/9122"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9119"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9116"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9116"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}