{"id":9080,"date":"2023-03-20T16:01:31","date_gmt":"2023-03-20T22:01:31","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&#038;p=9080"},"modified":"2023-03-20T20:55:46","modified_gmt":"2023-03-21T02:55:46","slug":"fluo-4-am-ultrapure-98","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/fluo-4-am-ultrapure-98\/","title":{"rendered":"Indicador Fluo-4 AM Ultrapuro (>98%)"},"content":{"rendered":"\n<p>Fluo-4 AM Ultrapure es nuestro indicador de calcio fluorescente verde con grado de mayor pureza (&gt;98 %) disponible. Fluo-4 Ultrapure es el indicador que investigadores han confiado, con un m\u00ednimo de impurezas para brindar m\u00e1xima confianza y ser permeable a la membrana.  Es la soluci\u00f3n m\u00e1s popular para ensayos de flujo de calcio de alto rendimiento y ensayos GPCR.<\/p>\n\n\n\n<p>Probado y confiable durante d\u00e9cadas para medir el flujo de calcio intracelular en aplicaciones de microscop\u00eda de fluorescencia y detecci\u00f3n de alto rendimiento.<\/p>\n\n\n\n<div style=\"height:28px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-table\" style=\"font-size:18px\"><table class=\"has-black-color has-text-color has-background\" style=\"background:linear-gradient(0deg,rgb(238,238,238) 0%,rgb(255,255,255) 28%,rgb(249,249,249) 72%,rgb(169,184,195) 100%)\"><thead><tr><th>Catalogo<\/th><th>Producto<\/th><th>Presentaci\u00f3n<\/th><\/tr><\/thead><tbody><tr><td>ON-1043G<\/td><td>Fluo-4 AM Ultrapure (&gt;98%)<\/td><td>10 x 50 ug<\/td><\/tr><tr><td>ON-1043F<\/td><td>Fluo-4 AM Ultrapure (&gt;98%)<\/td><td>3 x 50 ug<\/td><\/tr><tr><td>ON-1043C<\/td><td>Fluo-4 AM Ultrapure (&gt;98%)<\/td><td>500 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:46px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-container-1 is-nowrap wp-block-group\">\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/drive.google.com\/file\/d\/1iVt9ETTK8ZGqIXh7K2prRMxhGiXCRwEI\/view?usp=sharing\" target=\"_blank\" rel=\"noopener\" title=\"\">SDS<\/a><\/p>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/drive.google.com\/file\/d\/1rVTK0IcIvBj9etnTkmJfvxaYvhmJRUEG\/view?usp=sharing\" target=\"_blank\" rel=\"noopener\" title=\"\">Protocol<\/a> <\/p>\n\n\n\n<figure class=\"wp-block-image\"><img src=\"https:\/\/images.aatbio.com\/dependencies\/icon_pdf.png\" alt=\"pdf\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/drive.google.com\/file\/d\/11eQGgoVI34R6OSukOpyWBsN4EmYj3MRG\/view?usp=sharing\" target=\"_blank\" rel=\"noopener\" title=\"\">Especificaciones<\/a><\/p>\n<\/div>\n\n\n\n<div style=\"height:70px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Informaci\u00f3n<\/mark><\/p>\n\n\n\n<figure class=\"wp-block-table\"><table><tbody><tr><td>Excitaci\u00f3n<\/td><td>490 nm <strong>&nbsp;<\/strong><img loading=\"lazy\" width=\"18\" height=\"18\" src=\"https:\/\/ionbiosciences.com\/wp-content\/uploads\/2021\/01\/490.png\" alt=\"\"><\/td><\/tr><tr><td>Emisi\u00f3n<\/td><td>515 nm <strong>&nbsp;<\/strong><img loading=\"lazy\" width=\"18\" height=\"18\" src=\"https:\/\/ionbiosciences.com\/wp-content\/uploads\/2021\/01\/515.png\" alt=\"\"><\/td><\/tr><tr><td>Kd<\/td><td>355 nM<\/td><\/tr><tr><td>Peso molecular <\/td><td>1097 g\/mol<\/td><\/tr><tr><td>Solubilidad<\/td><td>DMSO<\/td><\/tr><tr><td>Pureza<\/td><td>&gt;98%<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<div style=\"height:56px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p style=\"font-size:16px\"><\/p>\n\n\n\n<div style=\"height:74px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-116-1024x667.png\" alt=\"\" class=\"wp-image-9081\" width=\"707\" height=\"460\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-116-1024x667.png 1024w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-116-300x195.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-116-768x500.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-116-600x391.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-116-246x160.png 246w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-116.png 1172w\" sizes=\"(max-width: 707px) 100vw, 707px\" \/><figcaption><strong>Fig. 1<\/strong>  Datos de fluorescencia adquiridos utilizando una FlexStation (Ex. 485nm, Em. 525nm, Cutoff 515nm)  de Molecular Devices para todas las concentraciones de ATP. Se a\u00f1adi\u00f3 ATP a los 30 segundos a las c\u00e9lulas CHO Ki.<\/figcaption><\/figure>\n\n\n\n<div style=\"height:72px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-117.png\" alt=\"\" class=\"wp-image-9082\" width=\"584\" height=\"432\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-117.png 770w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-117-300x222.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-117-768x569.png 768w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-117-600x444.png 600w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-117-216x160.png 216w\" sizes=\"(max-width: 584px) 100vw, 584px\" \/><figcaption><strong>Fig. 2 <\/strong>  Im\u00e1genes de fluorescencia de c\u00e9lulas te\u00f1idas con Fluo-4 AM antes (-) y despu\u00e9s (+) de la adici\u00f3n de ionomicina, un ion\u00f3foro de calcio. La fluorescencia celular aumenta debido a la entrada de calcio.<\/figcaption><\/figure>\n\n\n\n<div style=\"height:86px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Productos Relacionados<\/mark><\/p>\n\n\n\n<div style=\"height:13px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-container-5 wp-block-columns\">\n<div class=\"wp-container-2 wp-block-column\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-118.png\" alt=\"\" class=\"wp-image-9083\" width=\"206\" height=\"206\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-118.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-118-150x150.png 150w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-118-160x160.png 160w\" sizes=\"(max-width: 206px) 100vw, 206px\" \/><figcaption>ICR-1 AM<\/figcaption><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-container-3 wp-block-column\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-119.png\" alt=\"\" class=\"wp-image-9084\" width=\"208\" height=\"208\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-119.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-119-150x150.png 150w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-119-160x160.png 160w\" sizes=\"(max-width: 208px) 100vw, 208px\" \/><figcaption>Fura-2 LR AM<\/figcaption><\/figure>\n<\/div>\n\n\n\n<div class=\"wp-container-4 wp-block-column\">\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-120.png\" alt=\"\" class=\"wp-image-9085\" width=\"211\" height=\"211\" srcset=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-120.png 300w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-120-150x150.png 150w, https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/image-120-160x160.png 160w\" sizes=\"(max-width: 211px) 100vw, 211px\" \/><figcaption>ICR-1 K<sup>+<\/sup> Salt<\/figcaption><\/figure>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:78px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>Garantia ON Assurance. La calidad de este producto esta garantizado. Si no est\u00e1 satisfecho con su pedido, trabajaremos para resolver de inmediato su inquietud de acuerdo con nuestros t\u00e9rminos y condiciones de ION Assurance.<\/p>\n\n\n\n<div style=\"height:36px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-container-6 is-nowrap wp-block-group\">\n<figure class=\"wp-block-image size-full\"><img loading=\"lazy\" width=\"241\" height=\"85\" src=\"https:\/\/nuevo22.cidsamexico.com\/wp-content\/uploads\/2023\/03\/ion-biosciences-logo-1.png\" alt=\"\" class=\"wp-image-9038\"\/><\/figure>\n<\/div>\n\n\n\n<div style=\"height:82px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p><mark style=\"background-color:rgba(0, 0, 0, 0)\" class=\"has-inline-color has-vivid-cyan-blue-color\">Referencias<\/mark><\/p>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/32047113\/\">Horning, K. J., Joshi, P., Nitin, R., Balachandran, R. C., Yanko, F. M., Kim, K., \u2026 &amp; Bowman, A. B. (2020). Identification of a selective manganese ionophore that enables nonlethal quantification of cellular manganese.&nbsp;<em>Journal of Biological Chemistry<\/em>,&nbsp;<em>295<\/em>(12), 3875-3890<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/10756976\/\" target=\"_blank\" rel=\"noreferrer noopener\">Gee KR, Brown KA, Chen WN, Bishop-Stewart J, Gray D, Johnson I. Chemical and physiological characterization of fluo-4 Ca(2+)-indicator dyes. Cell Calcium. 2000 Feb;27(2):97-106. doi: 10.1054\/ceca.1999.0095<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/23821298\/\">Bovo E, Dvornikov AV, Mazurek SR, de Tombe PP, Zima AV. Mechanisms of Ca\u00b2+ handling in zebrafish ventricular myocytes. Pflugers Arch. 2013 Dec;465(12):1775-84. doi: 10.1007\/s00424-013-1312-2. Epub 2013 Jul 3. PMID: 23821298; PMCID: PMC4138713.<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/20923853\/\">Rodriguez AL, Grier MD, Jones CK, Herman EJ, Kane AS, Smith RL, Williams R, Zhou Y, Marlo JE, Days EL, Blatt TN, Jadhav S, Menon UN, Vinson PN, Rook JM, Stauffer SR, Niswender CM, Lindsley CW, Weaver CD, Conn PJ. Discovery of novel allosteric modulators of metabotropic glutamate receptor subtype 5 reveals chemical and functional diversity and in vivo activity in rat behavioral models of anxiolytic and antipsychotic activity. Mol Pharmacol. 2010. 78(6):1105-23.<\/a><\/p>\n\n\n\n<hr class=\"wp-block-separator has-alpha-channel-opacity\"\/>\n\n\n\n<p><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/22368764\/\">Zixiu Xiang 1, Analisa D Thompson, John T Brogan, Michael L Schulte, Bruce J Melancon, Debbie Mi, L Michelle Lewis, Bende Zou, Liya Yang, Ryan Morrison, Tammy Santomango, Frank Byers, Katrina Brewer, Jonathan S Aldrich, Haibo Yu, Eric S Dawson, Min Li, Owen McManus, Carrie K Jones, J Scott Daniels, Corey R Hopkins, Ximin Simon Xie, P Jeffrey Conn, C David Weaver, Craig W Lindsley. The Discovery and Characterization of ML218: A Novel, Centrally Active T-Type Calcium Channel Inhibitor with Robust Effects in STN Neurons and in a Rodent Model of Parkinson\u2019s Disease. ACS Chem Neurosci. 2011. 21;2(12):730-742. doi: 10.1021\/cn200090z.<\/a><\/p>\n\n\n\n<div style=\"height:100px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Fluo-4 AM es el indicador de calcio intracelular fluorescente verde m\u00e1s popular y ahora est\u00e1 disponible con el grado de pureza m\u00e1s alto (>98 %).<\/p>\n","protected":false},"featured_media":9098,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9080"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":6,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9080\/revisions"}],"predecessor-version":[{"id":9115,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/9080\/revisions\/9115"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/9098"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=9080"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=9080"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}