{"id":8544,"date":"2023-03-13T17:46:10","date_gmt":"2023-03-13T23:46:10","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8544"},"modified":"2023-03-13T18:02:22","modified_gmt":"2023-03-14T00:02:22","slug":"ifluor-810-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-810-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 810 succinimidyl ester"},"content":{"rendered":"\n

Las im\u00e1genes de fluorescencia in vivo utilizan una c\u00e1mara sensible para detectar la emisi\u00f3n de fluorescencia de los fluor\u00f3foros en peque\u00f1os animales vivos de cuerpo entero. Para superar la atenuaci\u00f3n de fotones en el tejido vivo, generalmente se prefieren los fluor\u00f3foros con emisi\u00f3n prolongada en la regi\u00f3n infrarroja (IR). Los avances recientes en las estrategias de obtenci\u00f3n de im\u00e1genes y las t\u00e9cnicas de reportero para la obtenci\u00f3n de im\u00e1genes por fluorescencia in vivo incluyen enfoques novedosos para mejorar la especificidad y la afinidad de las sondas y para modular y amplificar la se\u00f1al en los sitios objetivo para aumentar la sensibilidad. Otros desarrollos emergentes tienen como objetivo lograr im\u00e1genes de fluorescencia in vivo de alta resoluci\u00f3n, multimodalidad y basadas en la vida \u00fatil. <\/p>\n\n\n\n

Nuestro iFluor\u00ae 810 est\u00e1 dise\u00f1ado para marcar prote\u00ednas y otras biomol\u00e9culas con fluorescencia infrarroja. Los conjugados preparados con iFluor\u00ae 810 tienen excitaci\u00f3n y emisi\u00f3n en el rango IR. La emisi\u00f3n de colorante iFluor\u00ae 810 est\u00e1 bien separada de los fluor\u00f3foros de rojo lejano com\u00fanmente utilizados, como Cy5, Cy7 o la aloficocianina (APC), lo que facilita el an\u00e1lisis multicolor. Este fluor\u00f3foro tambi\u00e9n es \u00fatil para aplicaciones de im\u00e1genes in vivo de animales peque\u00f1os u otras aplicaciones de im\u00e1genes que requieren detecci\u00f3n IR. <\/p>\n\n\n\n

El succinimidil \u00e9ster iFluor\u00ae 810 es reactivo con amina y se puede usar f\u00e1cilmente para conjugar biomol\u00e9culas que contienen amina, particularmente anticuerpos.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1389<\/td>iFluor\u00ae 810 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71389<\/td>iFluor\u00ae 810 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71526<\/td>iFluor\u00ae 810 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71576<\/td>iFluor\u00ae 810 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>1576.03<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.09<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.15<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>2500001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>811<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>822<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.051<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 810 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>63.451 \u00b5L<\/td>317.253 \u00b5L<\/td>634.506 \u00b5L<\/td>3.173 mL<\/td>6.345 mL<\/td><\/tr>
5 mM<\/strong><\/td>12.69 \u00b5L<\/td>63.451 \u00b5L<\/td>126.901 \u00b5L<\/td>634.506 \u00b5L<\/td>1.269 mL<\/td><\/tr>
10 mM<\/strong><\/td>6.345 \u00b5L<\/td>31.725 \u00b5L<\/td>63.451 \u00b5L<\/td>317.253 \u00b5L<\/td>634.506 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
\n\n\n\n
<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1<\/strong>. Los \u00e9steres de NHS de tinte fluorescente (o \u00e9steres de succinimidilo) son la herramienta m\u00e1s popular para conjugar tintes con un p\u00e9ptido, prote\u00edna, anticuerpo, oligonucle\u00f3tido modificado con amino o \u00e1cido nucleico. Los \u00e9steres de NHS reaccionan r\u00e1pidamente con las aminas primarias (R-NH2) de prote\u00ednas, oligonucle\u00f3tidos modificados con amina y otras mol\u00e9culas que contienen amina. Los conjugados de tinte resultantes son bastante estables. iFluor\u00ae 810 es uno de los tintes fluorescentes comerciales que tienen la longitud de onda de excitaci\u00f3n y emisi\u00f3n m\u00e1s larga.<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 405 succinimidyl ester<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
iFluor\u00ae 488 succinimidyl ester<\/a><\/td>491<\/td>516<\/td>750001<\/sup><\/td>0.91<\/sup><\/td>0.21<\/td>0.11<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 647 succinimidyl ester<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr>
iFluor\u00ae 680 succinimidyl ester<\/a><\/td>684<\/td>701<\/td>2200001<\/sup><\/td>0.231<\/sup><\/td>0.097<\/td>0.094<\/td><\/tr>
iFluor\u00ae 700 succinimidyl ester<\/a><\/td>690<\/td>713<\/td>2200001<\/sup><\/td>0.231<\/sup><\/td>0.09<\/td>0.04<\/td><\/tr>
iFluor\u00ae 750 succinimidyl ester<\/a><\/td>757<\/td>779<\/td>2750001<\/sup><\/td>0.121<\/sup><\/td>0.044<\/td>0.039<\/td><\/tr>
iFluor\u00ae 610 succinimidyl ester<\/a><\/td>610<\/td>628<\/td>1100001<\/sup><\/td>0.851<\/sup><\/td>0.32<\/td>0.49<\/td><\/tr>
iFluor\u00ae 710 succinimidyl ester<\/a><\/td>717<\/td>739<\/td>1900001<\/sup><\/td>0.601<\/sup><\/td>0.12<\/td>0.07<\/td><\/tr>
iFluor\u00ae 790 succinimidyl ester<\/a><\/td>787<\/td>812<\/td>2500001<\/sup><\/td>0.131<\/sup><\/td>0.1<\/td>0.09<\/td><\/tr>
iFluor\u00ae 800 succinimidyl ester<\/a><\/td>801<\/td>820<\/td>2500001<\/sup><\/td>0.111<\/sup><\/td>0.03<\/td>0.08<\/td><\/tr>
iFluor\u00ae 810 succinimidyl ester<\/a><\/td>811<\/td>822<\/td>2500001<\/sup><\/td>0.051<\/sup><\/td>0.09<\/td>0.15<\/td><\/tr>
iFluor\u00ae 820 succinimidyl ester<\/a><\/td>822<\/td>850<\/td>2500001<\/sup><\/td><\/td>0.11<\/td>0.16<\/td><\/tr>
iFluor\u00ae 860 succinimidyl ester<\/a><\/td>853<\/td>878<\/td>2500001<\/sup><\/td><\/td>0.1<\/td>0.14<\/td><\/tr>
iFluor\u00ae 546 succinimidyl ester<\/a><\/td>541<\/td>557<\/td>1000001<\/sup><\/td>0.671<\/sup><\/td>0.25<\/td>0.15<\/td><\/tr>
iFluor\u00ae 568 succinimidyl ester<\/a><\/td>568<\/td>587<\/td>1000001<\/sup><\/td>0.571<\/sup><\/td>0.34<\/td>0.15<\/td><\/tr>
iFluor\u00ae 430 succinimidyl ester<\/a><\/td>433<\/td>498<\/td>400001<\/sup><\/td>0.781<\/sup><\/td>0.68<\/td>0.3<\/td><\/tr>
iFluor\u00ae 450 succinimidyl ester<\/a><\/td>451<\/td>502<\/td>400001<\/sup><\/td>0.821<\/sup><\/td>0.45<\/td>0.27<\/td><\/tr>
iFluor\u00ae 840 succinimidyl ester<\/a><\/td>836<\/td>879<\/td>2000001<\/sup><\/td>–<\/td>0.2<\/td>0.09<\/td><\/tr>
iFluor\u00ae 560 succinimidyl ester<\/a><\/td>560<\/td>571<\/td>1200001<\/sup><\/td>0.571<\/sup><\/td>0.0482<\/td>0.069<\/td><\/tr>
iFluor\u00ae 670 succinimidyl ester<\/a><\/td>671<\/td>682<\/td>2000001<\/sup><\/td>0.551<\/sup><\/td>0.03<\/td>0.033<\/td><\/tr>
iFluor\u00ae 460 succinimidyl ester<\/a><\/td>468<\/td>493<\/td>800001<\/sup><\/td>~0.81<\/sup><\/td>0.98<\/td>0.46<\/td><\/tr>
iFluor\u00ae 440 succinimidyl ester<\/a><\/td>434<\/td>480<\/td>400001<\/sup><\/td>0.671<\/sup><\/td>0.352<\/td>0.229<\/td><\/tr>
iFluor\u00ae 665 succinimidyl ester<\/a><\/td>667<\/td>692<\/td>110,0001<\/sup><\/td>0.221<\/sup><\/td>0.12<\/td>0.09<\/td><\/tr>
iFluor\u00ae 690 succinimidyl ester<\/a><\/td>685<\/td>704<\/td>2200001<\/sup><\/td>0.301<\/sup><\/td>0.09<\/td>0.06<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
iFluor\u00ae A7 SE<\/a><\/td><\/tr>
iFluor\u00ae 350 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 488 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 555 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 647 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 680 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 700 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 750 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 350 amine<\/a><\/td><\/tr>
iFluor\u00ae 405 amine<\/a><\/td><\/tr>
iFluor\u00ae 488 amine<\/a><\/td><\/tr>
iFluor\u00ae 555 amine<\/a><\/td><\/tr>
iFluor\u00ae 647 amine<\/a><\/td><\/tr>
iFluor\u00ae 660 amine<\/a><\/td><\/tr>
iFluor\u00ae 680 amine<\/a><\/td><\/tr>
iFluor\u00ae 700 amine<\/a><\/td><\/tr>
iFluor\u00ae 710 amine<\/a><\/td><\/tr>
iFluor\u00ae 750 amine<\/a><\/td><\/tr>
iFluor\u00ae 350 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 488 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 555 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 647 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 680 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 700 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 750 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 647 alkyne<\/a><\/td><\/tr>
iFluor\u00ae 647 azide<\/a><\/td><\/tr>
iFluor\u00ae 790 acid<\/a><\/td><\/tr>
iFluor\u00ae 790 amine<\/a><\/td><\/tr>
iFluor\u00ae 790 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 790 maleimide<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliografi\u00eda<\/mark><\/p>\n\n\n\n

Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution<\/a>
Authors: <\/strong>Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: <\/strong>Nanomedicine (2017)<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 18 references: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
Authors: <\/strong>Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.
Journal: <\/strong>Cancer Res (2007): 2791<\/p>\n\n\n\n

Fluorescence imaging in vivo: recent advances
Authors: <\/strong>Rao J, Dragulescu-Andrasi A, Yao H.
Journal: <\/strong>Curr Opin Biotechnol (2007): 17<\/p>\n\n\n\n

Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
Authors: <\/strong>Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.
Journal: <\/strong>Photochem Photobiol (2007): 1157<\/p>\n\n\n\n

In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis
Authors: <\/strong>Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.
Journal: <\/strong>J Immunol Methods (2007): 65<\/p>\n\n\n\n

A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice
Authors: <\/strong>Bonano VI, Oltean S, Garcia-Blanco MA.
Journal: <\/strong>Nat Protoc (2007): 2166<\/p>\n\n\n\n

In vivo imaging of the bronchial wall microstructure using fibered confocal fluorescence microscopy
Authors: <\/strong>Thiberville L, Moreno-Swirc S, Vercauteren T, Peltier E, Cave C, Bourg Heckly G.
Journal: <\/strong>Am J Respir Crit Care Med (2007): 22<\/p>\n\n\n\n

In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice
Authors: <\/strong>Szabo A, Vollmar B, Boros M, Menger MD.
Journal: <\/strong>J Surg Res. (2007)<\/p>\n\n\n\n

In vivo spectral fluorescence imaging of submillimeter peritoneal cancer implants using a lectin-targeted optical agent
Authors: <\/strong>Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Krishna MC, Choyke PL, Kobayashi H.
Journal: <\/strong>Neoplasia (2006): 607<\/p>\n\n\n\n

In vivo imaging of green fluorescent protein-expressing cells in transgenic animals using fibred confocal fluorescence microscopy
Authors: <\/strong>Al-Gubory KH, Houdebine LM.
Journal: <\/strong>Eur J Cell Biol (2006): 837<\/p>\n\n\n\n

In vivo near-infrared fluorescence imaging of integrin alphavbeta3 in an orthotopic glioblastoma model
Authors: <\/strong>Hsu AR, Hou LC, Veeravagu A, Greve JM, Vogel H, Tse V, Chen X.
Journal: <\/strong>Mol Imaging Biol (2006): 315<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes <\/mark><\/p>\n\n\n\n

iFluor\u00ae Dye Selection Guide<\/a> (Guia de selecci\u00f3n)
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

What are the spectral properties of iFluor dyes?<\/a>
Are any of the cyanine dyes infrared?<\/a>
Are coumarin dyes pH sensitive?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a>
Are there any alternatives to Cy5?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise<\/mark><\/p>\n\n\n\n

A practical guide for use of PE and APC in flow cytometry<\/a>
Calbryte\u2122 series now available<\/a>
Buccutite\u2122 Fluorescent Protein and Tandem Dye Antibody Labeling Kits<\/a>
Fundamentals of Flow Cytometry<\/a>
ReadiUse\u2122 Lyophilized Phycobiliproteins<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

El succinimidil \u00e9ster iFluor\u00ae 810 es reactivo con amina y se puede usar f\u00e1cilmente para conjugar biomol\u00e9culas que contienen amina, particularmente anticuerpos.<\/p>\n","protected":false},"featured_media":8541,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8544"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8544\/revisions"}],"predecessor-version":[{"id":8554,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8544\/revisions\/8554"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8541"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8544"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8544"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}