{"id":8504,"date":"2023-03-12T22:49:48","date_gmt":"2023-03-13T04:49:48","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8504"},"modified":"2023-03-12T23:00:14","modified_gmt":"2023-03-13T05:00:14","slug":"ifluor-670-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-670-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 670 succinimidyl ester"},"content":{"rendered":"\n

Los tintes iFluor\u00ae de AAT Bioquest est\u00e1n optimizados para marcar prote\u00ednas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento m\u00ednimo de las prote\u00ednas. Pueden excitarse bien con las principales l\u00edneas l\u00e1ser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm). <\/p>\n\n\n\n

La familia iFluor\u00ae 670 tiene propiedades espectrales casi id\u00e9nticas a las de Cy5B. En comparaci\u00f3n con Cy5B, iFluor\u00ae 670 tiene un rendimiento cu\u00e1ntico de fluorescencia mucho mayor con una fotoestabilidad muy mejorada. Adem\u00e1s, la fluorescencia de iFluor\u00ae 670 es insensible al pH en un amplio rango, pH 3-11. Estas caracter\u00edsticas espectrales hacen de esta nueva familia de colorantes una alternativa superior a Cy5B. <\/p>\n\n\n\n

iFluor\u00ae 670 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1033<\/td>iFluor\u00ae 670 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71033<\/td>iFluor\u00ae 670 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71512<\/td>iFluor\u00ae 670 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71562<\/td>iFluor\u00ae 670 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>923.15<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.03<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.033<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>2000001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>671<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>682<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.551<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 670 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>108.325 \u00b5L<\/td>541.624 \u00b5L<\/td>1.083 mL<\/td>5.416 mL<\/td>10.832 mL<\/td><\/tr>
5 mM<\/strong><\/td>21.665 \u00b5L<\/td>108.325 \u00b5L<\/td>216.65 \u00b5L<\/td>1.083 mL<\/td>2.166 mL<\/td><\/tr>
10 mM<\/strong><\/td>10.832 \u00b5L<\/td>54.162 \u00b5L<\/td>108.325 \u00b5L<\/td>541.624 \u00b5L<\/td>1.083 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1<\/strong>. Las c\u00e9lulas HeLa se incubaron con (+ Tubulin) o sin (-Tubulin) anti-tubulina de rat\u00f3n seguido de iFluor\u00ae 670 tinci\u00f3n de conjugado de IgG anti-rat\u00f3n de cabra y se visualizaron con Cy5 Filter.<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 405 succinimidyl ester<\/a><\/td>403<\/td>427<\/td>370001<\/sup><\/td>0.911<\/sup><\/td>0.48<\/td>0.77<\/td><\/tr>
iFluor\u00ae 488 succinimidyl ester<\/a><\/td>491<\/td>516<\/td>750001<\/sup><\/td>0.91<\/sup><\/td>0.21<\/td>0.11<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 647 succinimidyl ester<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr>
iFluor\u00ae 680 succinimidyl ester<\/a><\/td>684<\/td>701<\/td>2200001<\/sup><\/td>0.231<\/sup><\/td>0.097<\/td>0.094<\/td><\/tr>
iFluor\u00ae 700 succinimidyl ester<\/a><\/td>690<\/td>713<\/td>2200001<\/sup><\/td>0.231<\/sup><\/td>0.09<\/td>0.04<\/td><\/tr>
iFluor\u00ae 750 succinimidyl ester<\/a><\/td>757<\/td>779<\/td>2750001<\/sup><\/td>0.121<\/sup><\/td>0.044<\/td>0.039<\/td><\/tr>
iFluor\u00ae 610 succinimidyl ester<\/a><\/td>610<\/td>628<\/td>1100001<\/sup><\/td>0.851<\/sup><\/td>0.32<\/td>0.49<\/td><\/tr>
iFluor\u00ae 710 succinimidyl ester<\/a><\/td>717<\/td>739<\/td>1900001<\/sup><\/td>0.601<\/sup><\/td>0.12<\/td>0.07<\/td><\/tr>
iFluor\u00ae 790 succinimidyl ester<\/a><\/td>787<\/td>812<\/td>2500001<\/sup><\/td>0.131<\/sup><\/td>0.1<\/td>0.09<\/td><\/tr>
iFluor\u00ae 800 succinimidyl ester<\/a><\/td>801<\/td>820<\/td>2500001<\/sup><\/td>0.111<\/sup><\/td>0.03<\/td>0.08<\/td><\/tr>
iFluor\u00ae 810 succinimidyl ester<\/a><\/td>811<\/td>822<\/td>2500001<\/sup><\/td>0.051<\/sup><\/td>0.09<\/td>0.15<\/td><\/tr>
iFluor\u00ae 820 succinimidyl ester<\/a><\/td>822<\/td>850<\/td>2500001<\/sup><\/td><\/td>0.11<\/td>0.16<\/td><\/tr>
iFluor\u00ae 860 succinimidyl ester<\/a><\/td>853<\/td>878<\/td>2500001<\/sup><\/td><\/td>0.1<\/td>0.14<\/td><\/tr>
iFluor\u00ae 546 succinimidyl ester<\/a><\/td>541<\/td>557<\/td>1000001<\/sup><\/td>0.671<\/sup><\/td>0.25<\/td>0.15<\/td><\/tr>
iFluor\u00ae 568 succinimidyl ester<\/a><\/td>568<\/td>587<\/td>1000001<\/sup><\/td>0.571<\/sup><\/td>0.34<\/td>0.15<\/td><\/tr>
iFluor\u00ae 430 succinimidyl ester<\/a><\/td>433<\/td>498<\/td>400001<\/sup><\/td>0.781<\/sup><\/td>0.68<\/td>0.3<\/td><\/tr>
iFluor\u00ae 450 succinimidyl ester<\/a><\/td>451<\/td>502<\/td>400001<\/sup><\/td>0.821<\/sup><\/td>0.45<\/td>0.27<\/td><\/tr>
iFluor\u00ae 840 succinimidyl ester<\/a><\/td>836<\/td>879<\/td>2000001<\/sup><\/td>–<\/td>0.2<\/td>0.09<\/td><\/tr>
iFluor\u00ae 560 succinimidyl ester<\/a><\/td>560<\/td>571<\/td>1200001<\/sup><\/td>0.571<\/sup><\/td>0.0482<\/td>0.069<\/td><\/tr>
iFluor\u00ae 670 succinimidyl ester<\/a><\/td>671<\/td>682<\/td>2000001<\/sup><\/td>0.551<\/sup><\/td>0.03<\/td>0.033<\/td><\/tr>
iFluor\u00ae 460 succinimidyl ester<\/a><\/td>468<\/td>493<\/td>800001<\/sup><\/td>~0.81<\/sup><\/td>0.98<\/td>0.46<\/td><\/tr>
iFluor\u00ae 440 succinimidyl ester<\/a><\/td>434<\/td>480<\/td>400001<\/sup><\/td>0.671<\/sup><\/td>0.352<\/td>0.229<\/td><\/tr>
iFluor\u00ae 665 succinimidyl ester<\/a><\/td>667<\/td>692<\/td>110,0001<\/sup><\/td>0.221<\/sup><\/td>0.12<\/td>0.09<\/td><\/tr>
iFluor\u00ae 690 succinimidyl ester<\/a><\/td>685<\/td>704<\/td>2200001<\/sup><\/td>0.301<\/sup><\/td>0.09<\/td>0.06<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
iFluor\u00ae A7 SE<\/a><\/td><\/tr>
iFluor\u00ae 350 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 488 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 555 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 647 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 680 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 700 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 750 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 350 amine<\/a><\/td><\/tr>
iFluor\u00ae 405 amine<\/a><\/td><\/tr>
iFluor\u00ae 488 amine<\/a><\/td><\/tr>
iFluor\u00ae 555 amine<\/a><\/td><\/tr>
iFluor\u00ae 647 amine<\/a><\/td><\/tr>
iFluor\u00ae 660 amine<\/a><\/td><\/tr>
iFluor\u00ae 680 amine<\/a><\/td><\/tr>
iFluor\u00ae 700 amine<\/a><\/td><\/tr>
iFluor\u00ae 710 amine<\/a><\/td><\/tr>
iFluor\u00ae 750 amine<\/a><\/td><\/tr>
iFluor\u00ae 350 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 488 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 555 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 647 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 680 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 700 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 750 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 647 alkyne<\/a><\/td><\/tr>
iFluor\u00ae 647 azide<\/a><\/td><\/tr>
iFluor\u00ae 790 acid<\/a><\/td><\/tr>
iFluor\u00ae 790 amine<\/a><\/td><\/tr>
iFluor\u00ae 790 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 790 maleimide<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliografi\u00eda<\/mark><\/p>\n\n\n\n

A combined solvatochromic shift and TDDFT study probing solute-solvent interactions of blue fluorescent Alexa Fluor 350 dye: Evaluation of ground and excited state dipole moments
Authors: <\/strong>Patil, M. K., Kotresh, M. G., Inamdar, S. R.
Journal: <\/strong>Spectrochim Acta A Mol Biomol Spectrosc (2019): 142-152<\/p>\n\n\n\n

Photobleaching Comparison of R-Phycoerythrin-Streptavidin and Streptavidin-Alexa Fluor 568 in a Breast Cancer Cell Line
Authors: <\/strong>Ostad, S. N., Babaei, S., Bayat, A. A., Mahmoudian, J.
Journal: <\/strong>Monoclon Antib Immunodiagn Immunother (2019): 25-29<\/p>\n\n\n\n

Comparison between photostability of Alexa Fluor 448 and Alexa Fluor 647 with conventional dyes FITC and APC by flow cytometry
Authors: <\/strong>Rai, S., Bhardwaj, U., Misra, A., Singh, S., Gupta, R.
Journal: <\/strong>Int J Lab Hematol (2018): e52-e54<\/p>\n\n\n\n

Development of new hCaM-Alexa Fluor((R)) biosensors for a wide range of ligands
Authors: <\/strong>Velazquez-Lopez, I., Leon-Cruz, E., Pardo, J. P., Sosa-Peinado, A., Gonzalez-Andrade, M.
Journal: <\/strong>Anal Biochem (2017): 13-22<\/p>\n\n\n\n

Neuroanatomical basis of clinical joint application of &quot;Jinggu&quot; (BL 64, a source-acupoint) and &quot;Dazhong&quot; (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: <\/strong>Cui, J. J., Zhu, X. L., Ji, C. F., Jing, X. H., Bai, W. Z.
Journal: <\/strong>Zhen Ci Yan Jiu (2011): 262-7<\/p>\n\n\n\n

Neuroanatomical characteristics of acupoint &quot;Chengshan&quot; (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: <\/strong>Zhu, X. L., Bai, W. Z., Wu, F. D., Jiang, J., Jing, X. H.
Journal: <\/strong>Zhen Ci Yan Jiu (2010): 433-7<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Improved fluoroimmunoassays using the dye Alexa Fluor 647 with the RAPTOR, a fiber optic biosensor
Authors: <\/strong>Anderson GP, Nerurkar NL.
Journal: <\/strong>J Immunol Methods (2002): 17<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes <\/mark><\/p>\n\n\n\n

iFluor\u00ae Dye Selection Guide<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

What are the spectral properties of iFluor dyes?<\/a>
Are any of the cyanine dyes infrared?<\/a>
Are coumarin dyes pH sensitive?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a>
Are there any alternatives to Cy5?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise<\/mark><\/p>\n\n\n\n

A practical guide for use of PE and APC in flow cytometry<\/a>
Calbryte\u2122 series now available<\/a>
Buccutite\u2122 Fluorescent Protein and Tandem Dye Antibody Labeling Kits<\/a>
Fundamentals of Flow Cytometry<\/a>
ReadiUse\u2122 Lyophilized Phycobiliproteins<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

La nueva familia de colorantes iFluor 660 son una excelente alternativa al Cy5B<\/p>\n","protected":false},"featured_media":8506,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8504"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8504\/revisions"}],"predecessor-version":[{"id":8509,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8504\/revisions\/8509"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8506"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8504"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8504"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}