{"id":8463,"date":"2023-03-11T16:55:13","date_gmt":"2023-03-11T22:55:13","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8463"},"modified":"2023-03-11T17:17:31","modified_gmt":"2023-03-11T23:17:31","slug":"ifluor-560-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-560-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 560 succinimidyl ester"},"content":{"rendered":"\n

Cy3 es el tinte menos fluorescente entre todos los tintes Cy. Cy3B era una versi\u00f3n mejorada del tinte Cy3 con un rendimiento cu\u00e1ntico de fluorescencia y una fotoestabilidad significativamente mayores. Sin embargo, Cy3B tiene una solubilidad en agua extremadamente baja, lo que dificulta el etiquetado de biomol\u00e9culas, aunque ha mejorado mucho la fluorescencia. <\/p>\n\n\n\n

iFluor\u00ae 560 tiene propiedades espectrales casi id\u00e9nticas a Cy3B. En comparaci\u00f3n con Cy3B, iFluor\u00ae 560 tiene una solubilidad en agua significativamente mejorada.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1040<\/td>iFluor\u00ae 560 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71040<\/td>iFluor\u00ae 560 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71518<\/td>iFluor\u00ae 560 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71568<\/td>iFluor\u00ae 560 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>1257.45<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.0482<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.069<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>1200001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>560<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>571<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.571<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 560 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>79.526 \u00b5L<\/td>397.63 \u00b5L<\/td>795.26 \u00b5L<\/td>3.976 mL<\/td>7.953 mL<\/td><\/tr>
5 mM<\/strong><\/td>15.905 \u00b5L<\/td>79.526 \u00b5L<\/td>159.052 \u00b5L<\/td>795.26 \u00b5L<\/td>1.591 mL<\/td><\/tr>
10 mM<\/strong><\/td>7.953 \u00b5L<\/td>39.763 \u00b5L<\/td>79.526 \u00b5L<\/td>397.63 \u00b5L<\/td>795.26 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
\n\n\n\n
<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1<\/strong>. Las c\u00e9lulas HeLa se incubaron con (+ Tubulin) o sin (-Tubulin) anti-tubulina de rat\u00f3n seguido de iFluor\u00ae 560 tinci\u00f3n de conjugado de IgG anti-rat\u00f3n de cabra y se visualizaron con Cy3 Filter.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 488 succinimidyl ester<\/a><\/td>491<\/td>516<\/td>750001<\/sup><\/td>0.91<\/sup><\/td>0.21<\/td>0.11<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 594 succinimidyl ester<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 647 succinimidyl ester<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
iFluor\u00ae A7 SE<\/a><\/td><\/tr>
iFluor\u00ae 350 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 488 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 555 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 647 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 680 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 700 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 750 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 350 amine<\/a><\/td><\/tr>
iFluor\u00ae 488 amine<\/a><\/td><\/tr>
iFluor\u00ae 555 amine<\/a><\/td><\/tr>
iFluor\u00ae 647 amine<\/a><\/td><\/tr>
iFluor\u00ae 660 amine<\/a><\/td><\/tr>
iFluor\u00ae 680 amine<\/a><\/td><\/tr>
iFluor\u00ae 700 amine<\/a><\/td><\/tr>
iFluor\u00ae 710 amine<\/a><\/td><\/tr>
iFluor\u00ae 750 amine<\/a><\/td><\/tr>
iFluor\u00ae 350 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 488 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 555 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 647 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 680 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 700 hydrazide<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Application Notes <\/mark><\/p>\n\n\n\n

iFluor\u00ae Dye Selection Guide<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

What are the spectral properties of iFluor dyes?<\/a>
Are any of the cyanine dyes infrared?<\/a>
Are coumarin dyes pH sensitive?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a>
Are there any alternatives to Cy5?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise<\/mark><\/p>\n\n\n\n

A practical guide for use of PE and APC in flow cytometry<\/a>
Calbryte\u2122 series now available<\/a>
Buccutite\u2122 Fluorescent Protein and Tandem Dye Antibody Labeling Kits<\/a>
Fundamentals of Flow Cytometry<\/a>
ReadiUse\u2122 Lyophilized Phycobiliproteins<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

iFluor\u00ae 560 tiene una solubilidad en agua significativamente mejorada.<\/p>\n","protected":false},"featured_media":8465,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8463"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8463\/revisions"}],"predecessor-version":[{"id":8468,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8463\/revisions\/8468"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8465"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8463"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8463"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}