{"id":8433,"date":"2023-03-11T14:08:15","date_gmt":"2023-03-11T20:08:15","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8433"},"modified":"2023-03-11T14:23:13","modified_gmt":"2023-03-11T20:23:13","slug":"ifluor-440-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-440-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 440 succinimidyl ester"},"content":{"rendered":"\n

Los tintes iFluor\u00ae de AAT Bioquest est\u00e1n optimizados para marcar prote\u00ednas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento m\u00ednimo de las prote\u00ednas. Pueden excitarse bien con las principales l\u00edneas l\u00e1ser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm). <\/p>\n\n\n\n

Los tintes iFluor\u00ae 440 est\u00e1n dise\u00f1ados para ser un reemplazo superior del tinte DEAC que tiene una solubilidad en agua extremadamente baja. iFluor\u00ae 440 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas. En las mismas condiciones, los conjugados de colorantes iFluor\u00ae 440 son significativamente m\u00e1s brillantes que los correspondientes bioconjugados de DEAC u otros colorantes espectralmente similares (como SpectrumAqua), lo que hace que los conjugados iFluor\u00ae 440 sean mucho m\u00e1s sensibles.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1041<\/td>iFluor\u00ae 440 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71041<\/td>iFluor\u00ae 440 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71519<\/td>iFluor\u00ae 440 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71569<\/td>iFluor\u00ae 440 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>692.83<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades espectrales<\/mark><\/p>\n\n\n\n

Absorbancia (nm)<\/td>430<\/td><\/tr>
Factor de correcci\u00f3n (260 nm)<\/td>0.352<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.229<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>400001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>434<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>480<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.671<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 440 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>144.336 \u00b5L<\/td>721.678 \u00b5L<\/td>1.443 mL<\/td>7.217 mL<\/td>14.434 mL<\/td><\/tr>
5 mM<\/strong><\/td>28.867 \u00b5L<\/td>144.336 \u00b5L<\/td>288.671 \u00b5L<\/td>1.443 mL<\/td>2.887 mL<\/td><\/tr>
10 mM<\/strong><\/td>14.434 \u00b5L<\/td>72.168 \u00b5L<\/td>144.336 \u00b5L<\/td>721.678 \u00b5L<\/td>1.443 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1<\/strong>. Las c\u00e9lulas HeLa se incubaron con antitubulina de rat\u00f3n seguida de conjugado de IgG anti-rat\u00f3n de cabra iFluorTM 430 de AAT (izquierda) o IgG anti-rat\u00f3n de cabra conjugado con Alexa Fluor\u00ae 430 (derecha), respectivamente.<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 488 succinimidyl ester<\/a><\/td>491<\/td>516<\/td>750001<\/sup><\/td>0.91<\/sup><\/td>0.21<\/td>0.11<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 594 succinimidyl ester<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 647 succinimidyl ester<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
iFluor\u00ae A7 SE<\/a><\/td><\/tr>
iFluor\u00ae 350 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 488 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 555 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 647 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 680 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 700 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 750 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 350 amine<\/a><\/td><\/tr>
iFluor\u00ae 488 amine<\/a><\/td><\/tr>
iFluor\u00ae 555 amine<\/a><\/td><\/tr>
iFluor\u00ae 647 amine<\/a><\/td><\/tr>
iFluor\u00ae 660 amine<\/a><\/td><\/tr>
iFluor\u00ae 680 amine<\/a><\/td><\/tr>
iFluor\u00ae 700 amine<\/a><\/td><\/tr>
iFluor\u00ae 710 amine<\/a><\/td><\/tr>
iFluor\u00ae 750 amine<\/a><\/td><\/tr>
iFluor\u00ae 350 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 488 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 555 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 647 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 680 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 700 hydrazide<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 12 referencias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

7-(Diethylamino)coumarin-3-carboxylic acid as derivatization reagent for 405\u202fnm laser-induced fluorescence detection: A case study for the analysis of sulfonamides by capillary electrophoresis.
Authors: <\/strong>Wu, Chengxin and Sun, Yuanyuan and Wang, Yuanhang and Duan, Wenzhen and Hu, Jiangyue and Zhou, Lei and Pu, Qiaosheng
Journal: <\/strong>Talanta (2019): 16-22<\/p>\n\n\n\n

Design, synthesis and perception of fluorescently labeled isoprenoid cytokinins.
Authors: <\/strong>Kubiasov\u00e1, Karolina and Mik, V\u00e1clav and Nisler, Jaroslav and H\u00f6nig, Martin and Husi\u010dkov\u00e1, Alexandra and Sp\u00edchal, Luk\u00e1\u0161 and P\u011bkn\u00e1, Zuzana and \u0160amajov\u00e1, Olga and Dole\u017eal, Karel and Pl\u00edhal, Ond\u0159ej and Benkov\u00e1, Eva and Strnad, Miroslav and Pl\u00edhalov\u00e1, Lucie
Journal: <\/strong>Phytochemistry (2018): 1-11<\/p>\n\n\n\n

Intracellular Uncaging of cGMP with Blue Light.
Authors: <\/strong>Agarwal, Hitesh K and Zhai, Shenyu and Surmeier, D James and Ellis-Davies, Graham C R
Journal: <\/strong>ACS chemical neuroscience (2017): 2139-2144<\/p>\n\n\n\n

Wavelength-selective one- and two-photon uncaging of GABA.
Authors: <\/strong>Amatrudo, Joseph M and Olson, Jeremy P and Lur, G and Chiu, Chiayu Q and Higley, Michael J and Ellis-Davies, Graham C R
Journal: <\/strong>ACS chemical neuroscience (2014): 64-70<\/p>\n\n\n\n

A water soluble fluorescent polymer as a dual colour sensor for temperature and a specific protein.
Authors: <\/strong>Inal, Sahika and K\u00f6lsch, Jonas D and Sellrie, Frank and Schenk, J\u00f6rg A and Wischerhoff, Erik and Laschewsky, Andr\u00e9 and Neher, Dieter
Journal: <\/strong>Journal of materials chemistry. B (2013): 6373-6381<\/p>\n\n\n\n

Structures, synthesis, and human Nod1 stimulation of immunostimulatory bacterial peptidoglycan fragments in the environment.
Authors: <\/strong>Fujimoto, Yukari and Fukase, Koichi
Journal: <\/strong>Journal of natural products (2011): 518-25<\/p>\n\n\n\n

Fluorescent agonists for the Torpedo nicotinic acetylcholine receptor.
Authors: <\/strong>Krieger, Florian and Mourot, Alexandre and Araoz, Romulo and Kotzyba-Hibert, Florence and Molg\u00f3, Jordi and Bamberg, Ernst and Goeldner, Maurice
Journal: <\/strong>Chembiochem : a European journal of chemical biology (2008): 1146-53<\/p>\n\n\n\n

Antimicrobial activity of various cationic molecules on foodborne pathogens.
Authors: <\/strong>Conte, Mariachiara and Aliberti, Francesco and Fucci, Laura and Piscopo, Marina
Journal: <\/strong>World journal of microbiology & biotechnology (2007): 1679-83<\/p>\n\n\n\n

A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin.
Authors: <\/strong>Webb, Martin R and Reid, Gordon P and Munasinghe, V Ranjit N and Corrie, John E T
Journal: <\/strong>Biochemistry (2004): 14463-71<\/p>\n\n\n\n

Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin.
Authors: <\/strong>Webb, M R and Corrie, J E
Journal: <\/strong>Biophysical journal (2001): 1562-9<\/p>\n\n\n\n

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Application Notes <\/mark><\/p>\n\n\n\n

i<\/a>Fluor\u00ae Dye Selection Gui<\/a>de<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

Are any of the cyanine dyes infrared?<\/a>
Are coumarin dyes pH sensitive?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a>
Are there any alternatives to Cy5?<\/a>
Are there any alternatives to indocyanine green (ICG)?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise<\/mark><\/p>\n\n\n\n

A practical guide for use of PE and APC in flow cytometry<\/a>
Calbryte\u2122 series now available<\/a>
Buccutite\u2122 Fluorescent Protein and Tandem Dye Antibody Labeling Kits<\/a>
Fundamentals of Flow Cytometry<\/a>
ReadiUse\u2122 Lyophilized Phycobiliproteins<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Los conjugados de colorantes iFluor\u00ae 440 son significativamente m\u00e1s brillantes que los correspondientes bioconjugados de DEAC u otros colorantes espectralmente similares (como SpectrumAqua).<\/p>\n","protected":false},"featured_media":2449,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8433"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8433\/revisions"}],"predecessor-version":[{"id":8437,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8433\/revisions\/8437"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/2449"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8433"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8433"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}