{"id":8428,"date":"2023-03-11T13:54:07","date_gmt":"2023-03-11T19:54:07","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8428"},"modified":"2023-03-11T14:06:31","modified_gmt":"2023-03-11T20:06:31","slug":"ifluor-430-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-430-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 430 succinimidyl ester"},"content":{"rendered":"\n

Los tintes iFluor\u00ae de AAT Bioquest est\u00e1n optimizados para marcar prote\u00ednas, en particular anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento m\u00ednimo de las prote\u00ednas. Pueden excitarse bien con las principales l\u00edneas l\u00e1ser de los instrumentos de fluorescencia (p. ej., 350, 405, 488, 555 y 633 nm). <\/p>\n\n\n\n

Los tintes iFluor\u00ae 430 est\u00e1n dise\u00f1ados para ser un reemplazo superior del tinte de etiquetado Alexa Fluor\u00ae 430 (Alexa Fluor\u00ae es la marca registrada de Invitrogen). iFluor\u00ae 430 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas. En las mismas condiciones, los conjugados de tinte iFluor\u00ae 430 son significativamente m\u00e1s brillantes que los bioconjugados correspondientes de Alexa Fluor 430 con una absorci\u00f3n mucho m\u00e1s fuerte, lo que hace que los conjugados iFluor 430 sean mucho m\u00e1s sensibles.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1052<\/td>iFluor\u00ae 430 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71052<\/td>iFluor\u00ae 430 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71523<\/td>iFluor\u00ae 430 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71573<\/td>iFluor\u00ae 430 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>688.79<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en\u00a0Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.68<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.3<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>400001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>433<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>498<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.781<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 430 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>145.182 \u00b5L<\/td>725.911 \u00b5L<\/td>1.452 mL<\/td>7.259 mL<\/td>14.518 mL<\/td><\/tr>
5 mM<\/strong><\/td>29.036 \u00b5L<\/td>145.182 \u00b5L<\/td>290.364 \u00b5L<\/td>1.452 mL<\/td>2.904 mL<\/td><\/tr>
10 mM<\/strong><\/td>14.518 \u00b5L<\/td>72.591 \u00b5L<\/td>145.182 \u00b5L<\/td>725.911 \u00b5L<\/td>1.452 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1<\/strong>. Las c\u00e9lulas HeLa se incubaron con antitubulina de rat\u00f3n seguida de conjugado de IgG anti-rat\u00f3n de cabra iFluorTM 430 de AAT (izquierda) o IgG anti-rat\u00f3n de cabra conjugado con Alexa Fluor\u00ae 430 (derecha), respectivamente.<\/p>\n\n\n\n

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Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 488 succinimidyl ester<\/a><\/td>491<\/td>516<\/td>750001<\/sup><\/td>0.91<\/sup><\/td>0.21<\/td>0.11<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 594 succinimidyl ester<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 647 succinimidyl ester<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
iFluor\u00ae A7 SE<\/a><\/td><\/tr>
iFluor\u00ae 350 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 488 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 555 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 647 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 680 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 700 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 750 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 350 amine<\/a><\/td><\/tr>
iFluor\u00ae 488 amine<\/a><\/td><\/tr>
iFluor\u00ae 555 amine<\/a><\/td><\/tr>
iFluor\u00ae 647 amine<\/a><\/td><\/tr>
iFluor\u00ae 660 amine<\/a><\/td><\/tr>
iFluor\u00ae 680 amine<\/a><\/td><\/tr>
iFluor\u00ae 700 amine<\/a><\/td><\/tr>
iFluor\u00ae 710 amine<\/a><\/td><\/tr>
iFluor\u00ae 750 amine<\/a><\/td><\/tr>
iFluor\u00ae 350 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 488 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 555 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 647 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 680 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 700 hydrazide<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification<\/a>
Authors: <\/strong>Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: <\/strong>Metabolomics (Los Angel) (2017): 2153–0769<\/p>\n\n\n\n

Suramin inhibits cullin-RING E3 ubiquitin ligases<\/a>
Authors: <\/strong>Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: <\/strong>Proceedings of the National Academy of Sciences (2016): E2011–E2018<\/p>\n\n\n\n

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function<\/a>
Authors: <\/strong>Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: <\/strong>International Journal of Cancer (2012): E425–E436<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 49 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: <\/strong>Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: <\/strong>Mol Biosyst (2011): 1766<\/p>\n\n\n\n

Visualizing dengue virus through Alexa Fluor labeling
Authors: <\/strong>Zhang S, Tan HC, Ooi EE.
Journal: <\/strong>J Vis Exp. (2011)<\/p>\n\n\n\n

Fluorescent “Turn-on” system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: <\/strong>Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: <\/strong>Biochem Biophys Res Commun (2011): 211<\/p>\n\n\n\n

Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: <\/strong>Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: <\/strong>Zhen Ci Yan Jiu (2011): 262<\/p>\n\n\n\n

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: <\/strong>Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: <\/strong>J Microbiol Methods (2011): 119<\/p>\n\n\n\n

Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
Authors: <\/strong>Hone AJ, Whiteaker P, Mohn JL, Jacob MH, McIntosh JM.
Journal: <\/strong>J Neurochem (2010): 994<\/p>\n\n\n\n

Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
Authors: <\/strong>Song F, Wang L, Qiao X, Wang B, Sun S, Fan J, Zhang L, Peng X.
Journal: <\/strong>Org Biomol Chem (2010): 4249<\/p>\n\n\n\n

Neuroanatomical characteristics of acupoint “Chengshan” (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: <\/strong>Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: <\/strong>Zhen Ci Yan Jiu (2010): 433<\/p>\n\n\n\n

Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: <\/strong>Maurel D, Banala S, Laroche T, Johnsson K.
Journal: <\/strong>ACS Chem Biol (2010): 507<\/p>\n\n\n\n

Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: <\/strong>Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: <\/strong>Biochem Pharmacol (2010): 506<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes <\/mark><\/p>\n\n\n\n

i<\/a>Fluor\u00ae Dye Selection Gui<\/a>de<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

What are common laser lines used in flow cytometry?<\/a>
What are the spectral properties of iFluor dyes?<\/a>
Are any of the cyanine dyes infrared?<\/a>
Are coumarin dyes pH sensitive?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise<\/mark><\/p>\n\n\n\n

A practical guide for use of PE and APC in flow cytometry<\/a>
Calbryte\u2122 series now available<\/a>
Buccutite\u2122 Fluorescent Protein and Tandem Dye Antibody Labeling Kits<\/a>
Fundamentals of Flow Cytometry<\/a>
ReadiUse\u2122 Lyophilized Phycobiliproteins<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Los conjugados de tinte iFluor\u00ae 430 son significativamente m\u00e1s brillantes que los bioconjugados correspondientes de Alexa Fluor 430 con una absorci\u00f3n mucho m\u00e1s fuerte, lo que hace que los conjugados iFluor 430 sean mucho m\u00e1s sensibles.<\/p>\n","protected":false},"featured_media":8430,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8428"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8428\/revisions"}],"predecessor-version":[{"id":8432,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8428\/revisions\/8432"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8430"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8428"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8428"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}