{"id":8418,"date":"2023-03-11T13:15:00","date_gmt":"2023-03-11T19:15:00","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8418"},"modified":"2023-03-11T13:53:24","modified_gmt":"2023-03-11T19:53:24","slug":"ifluor-405-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/ifluor-405-succinimidyl-ester\/","title":{"rendered":"iFluor\u00ae 405 succinimidyl ester"},"content":{"rendered":"\n

Los tintes iFluor\u00ae de AAT Bioquest est\u00e1n optimizados para marcar prote\u00ednas, en particular, anticuerpos. Estos tintes son brillantes, fotoestables y tienen un enfriamiento m\u00ednimo de las prote\u00ednas. Pueden excitarse bien con las principales l\u00edneas l\u00e1ser de los instrumentos de fluorescencia (ejem. 350, 405, 488, 555 y 633 nm). <\/p>\n\n\n\n

Los tintes iFluor\u00ae 405 tienen un m\u00e1ximo de excitaci\u00f3n y emisi\u00f3n de fluorescencia de ~406 nm y ~427 nm respectivamente. Estas caracter\u00edsticas espectrales los convierten en una excelente alternativa al colorante de etiquetado AMCA y Alexa Fluor\u00ae 405 (Alexa Fluor\u00ae es la marca registrada de Invitrogen). <\/p>\n\n\n\n

iFluor\u00ae 405 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-1021<\/td>iFluor\u00ae 405 succinimidyl ester<\/td>1mg<\/td><\/tr>
AAT-71021<\/td>iFluor\u00ae 405 succinimidyl ester<\/td>100 ug<\/td><\/tr>
AAT-71501<\/td>iFluor\u00ae 405 succinimidyl ester<\/td>5mg<\/td><\/tr>
AAT-71551<\/td>iFluor\u00ae 405 succinimidyl ester<\/td>10mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>755.58<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.48<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.77<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>370001<\/sup><\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>403<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>427<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.911<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Buffer acuoso (pH 7,2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de succinimidil \u00e9ster iFluor\u00ae 405 a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de la muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>132.349 \u00b5L<\/td>661.743 \u00b5L<\/td>1.323 mL<\/td>6.617 mL<\/td>13.235 mL<\/td><\/tr>
5 mM<\/strong><\/td>26.47 \u00b5L<\/td>132.349 \u00b5L<\/td>264.697 \u00b5L<\/td>1.323 mL<\/td>2.647 mL<\/td><\/tr>
10 mM<\/strong><\/td>13.235 \u00b5L<\/td>66.174 \u00b5L<\/td>132.349 \u00b5L<\/td>661.743 \u00b5L<\/td>1.323 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
\n\n\n\n
<\/div>\n\n\n\n

Imagenes<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1<\/strong>. Las c\u00e9lulas HL-60 se incubaron con (rojo, +) o sin (verde, -) anti-HLA-ABC humano (W6\/32 mAb), seguido de iFluor\u00ae 405 cabra anti-rat\u00f3n IgG (H&L). La se\u00f1al de fluorescencia se control\u00f3 utilizando un cit\u00f3metro de flujo ACEA NovoCyte en el canal Pacific Blue (Ex\/Em=405\/445 nm).<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Similares<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
iFluor\u00ae 350 succinimidyl ester<\/a><\/td>345<\/td>450<\/td>200001<\/sup><\/td>0.951<\/sup><\/td>0.83<\/td>0.23<\/td><\/tr>
iFluor\u00ae 488 succinimidyl ester<\/a><\/td>491<\/td>516<\/td>750001<\/sup><\/td>0.91<\/sup><\/td>0.21<\/td>0.11<\/td><\/tr>
iFluor\u00ae 514 succinimidyl ester<\/a><\/td>511<\/td>527<\/td>750001<\/sup><\/td>0.831<\/sup><\/td>0.265<\/td>0.116<\/td><\/tr>
iFluor\u00ae 532 succinimidyl ester<\/a><\/td>537<\/td>560<\/td>900001<\/sup><\/td>0.681<\/sup><\/td>0.26<\/td>0.16<\/td><\/tr>
iFluor\u00ae 555 succinimidyl ester<\/a><\/td>557<\/td>570<\/td>1000001<\/sup><\/td>0.641<\/sup><\/td>0.23<\/td>0.14<\/td><\/tr>
iFluor\u00ae 594 succinimidyl ester<\/a><\/td>588<\/td>604<\/td>1800001<\/sup><\/td>0.531<\/sup><\/td>0.05<\/td>0.04<\/td><\/tr>
iFluor\u00ae 633 succinimidyl ester<\/a><\/td>640<\/td>654<\/td>2500001<\/sup><\/td>0.291<\/sup><\/td>0.062<\/td>0.044<\/td><\/tr>
iFluor\u00ae 647 succinimidyl ester<\/a><\/td>656<\/td>670<\/td>2500001<\/sup><\/td>0.251<\/sup><\/td>0.03<\/td>0.03<\/td><\/tr>
iFluor\u00ae 660 succinimidyl ester<\/a><\/td>663<\/td>678<\/td>2500001<\/sup><\/td>0.261<\/sup><\/td>0.07<\/td>0.08<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
iFluor\u00ae 405 amine<\/a><\/td><\/tr>
iFluor\u00ae 405 maleimide<\/a><\/td><\/tr>
iFluor\u2122 405 azide<\/a><\/td><\/tr>
iFluor\u00ae 405 hydrazide<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
iFluor\u00ae A7 SE<\/a><\/td><\/tr>
iFluor\u00ae 350 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 488 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 555 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 647 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 680 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 700 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 750 maleimide<\/a><\/td><\/tr>
iFluor\u00ae 350 amine<\/a><\/td><\/tr>
iFluor\u00ae 488 amine<\/a><\/td><\/tr>
iFluor\u00ae 555 amine<\/a><\/td><\/tr>
iFluor\u00ae 647 amine<\/a><\/td><\/tr>
iFluor\u00ae 660 amine<\/a><\/td><\/tr>
iFluor\u00ae 680 amine<\/a><\/td><\/tr>
iFluor\u00ae 700 amine<\/a><\/td><\/tr>
iFluor\u00ae 710 amine<\/a><\/td><\/tr>
iFluor\u00ae 750 amine<\/a><\/td><\/tr>
iFluor\u00ae 350 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 488 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 555 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 647 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 680 hydrazide<\/a><\/td><\/tr>
iFluor\u00ae 700 hydrazide<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Arabidopsis cryptochrome 2 forms photobodies with TCP22 under blue light and regulates the circadian clock<\/a>
Authors: <\/strong>Mo, Weiliang and Zhang, Junchuan and Zhang, Li and Yang, Zhenming and Yang, Liang and Yao, Nan and Xiao, Yong and Li, Tianhong and Li, Yaxing and Zhang, Guangmei and others,
Journal: <\/strong>Nature communications (2022): 1–15<\/p>\n\n\n\n

Arc weakens synapses by dispersing AMPA receptors from postsynaptic density via modulating PSD phase separation<\/a>
Authors: <\/strong>Chen, Xudong and Jia, Bowen and Araki, Yoichi and Liu, Bian and Ye, Fei and Huganir, Richard and Zhang, Mingjie
Journal: <\/strong>Cell Research (2022): 914–930<\/p>\n\n\n\n

Kindlin2-mediated phase separation underlies integrin adhesion formation<\/a>
Authors: <\/strong>Li, Yujie and Zhang, Ting and Li, Huadong and Yang, Haibin and Lin, Ruihong and Sun, Kang and Wang, Lei and Zhang, Jing and Wei, Zhiyi and Yu, Cong
Journal: <\/strong>BioRxiv (2020)<\/p>\n\n\n\n

CaMKII activation triggers persistent formation and segregation of postsynaptic liquid phase<\/a>
Authors: <\/strong>Hosokawa, Tomohisa and Liu, Pin-Wu and Cai, Qixu and Ferreira, Joana S and Levet, Florian and Butler, Corey and Sibarita, Jean-Baptiste and Choquet, Daniel and Groc, Laurent and Hosy, Eric and others,
Journal: <\/strong>bioRxiv (2020)<\/p>\n\n\n\n

Par complex cluster formation mediated by phase separation<\/a>
Authors: <\/strong>Liu, Ziheng and Yang, Ying and Gu, Aihong and Xu, Jiawen and Mao, Ying and Lu, Haojie and Hu, Weiguo and Lei, Qun-Ying and Li, Zhouhua and Zhang, Mingjie and others,
Journal: <\/strong>Nature communications (2020): 1–18<\/p>\n\n\n\n

Phase separation-mediated TARP\/MAGUK complex condensation and AMPA receptor synaptic transmission
Authors: <\/strong>Zeng, Menglong and D{\\’\\i}az-Alonso, Javier and Ye, Fei and Chen, Xudong and Xu, Jia and Ji, Zeyang and Nicoll, Roger A and Zhang, Mingjie
Journal: <\/strong>Neuron (2019): 529–543<\/p>\n\n\n\n

Reconstituted postsynaptic density as a molecular platform for understanding synapse formation and plasticity<\/a>
Authors: <\/strong>Zeng, Menglong and Chen, Xudong and Guan, Dongshi and Xu, Jia and Wu, Haowei and Tong, Penger and Zhang, Mingjie
Journal: <\/strong>Cell (2018): 1172–1187<\/p>\n\n\n\n

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification<\/a>
Authors: <\/strong>Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: <\/strong>Metabolomics (Los Angel) (2017): 2153–0769<\/p>\n\n\n\n

Suramin inhibits cullin-RING E3 ubiquitin ligases<\/a>
Authors: <\/strong>Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: <\/strong>Proceedings of the National Academy of Sciences (2016): E2011–E2018<\/p>\n\n\n\n

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function<\/a>
Authors: <\/strong>Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: <\/strong>International Journal of Cancer (2012): E425–E436<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 49 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: <\/strong>Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: <\/strong>Mol Biosyst (2011): 1766<\/p>\n\n\n\n

Visualizing dengue virus through Alexa Fluor labeling
Authors: <\/strong>Zhang S, Tan HC, Ooi EE.
Journal: <\/strong>J Vis Exp. (2011)<\/p>\n\n\n\n

Fluorescent “Turn-on” system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: <\/strong>Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: <\/strong>Biochem Biophys Res Commun (2011): 211<\/p>\n\n\n\n

Neuroanatomical basis of clinical joint application of “Jinggu” (BL 64, a source-acupoint) and “Dazhong” (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: <\/strong>Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: <\/strong>Zhen Ci Yan Jiu (2011): 262<\/p>\n\n\n\n

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: <\/strong>Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: <\/strong>J Microbiol Methods (2011): 119<\/p>\n\n\n\n

Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
Authors: <\/strong>Hone AJ, Whiteaker P, Mohn JL, Jacob MH, McIntosh JM.
Journal: <\/strong>J Neurochem (2010): 994<\/p>\n\n\n\n

Asymmetric trimethine 3H-indocyanine dyes: efficient synthesis and protein labeling
Authors: <\/strong>Song F, Wang L, Qiao X, Wang B, Sun S, Fan J, Zhang L, Peng X.
Journal: <\/strong>Org Biomol Chem (2010): 4249<\/p>\n\n\n\n

Neuroanatomical characteristics of acupoint “Chengshan” (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: <\/strong>Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: <\/strong>Zhen Ci Yan Jiu (2010): 433<\/p>\n\n\n\n

Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: <\/strong>Maurel D, Banala S, Laroche T, Johnsson K.
Journal: <\/strong>ACS Chem Biol (2010): 507<\/p>\n\n\n\n

Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: <\/strong>Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: <\/strong>Biochem Pharmacol (2010): 506<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes <\/mark><\/p>\n\n\n\n

i<\/a>Fluor\u00ae Dye Selection Gui<\/a>de<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

What are common laser lines used in flow cytometry?<\/a>
What are the spectral properties of iFluor dyes?<\/a>
Are any of the cyanine dyes infrared?<\/a>
Are coumarin dyes pH sensitive?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise<\/mark><\/p>\n\n\n\n

A practical guide for use of PE and APC in flow cytometry<\/a>
Calbryte\u2122 series now available<\/a>
Buccutite\u2122 Fluorescent Protein and Tandem Dye Antibody Labeling Kits<\/a>
Fundamentals of Flow Cytometry<\/a>
ReadiUse\u2122 Lyophilized Phycobiliproteins<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

iFluor\u00ae 350 SE es razonablemente estable y muestra buena reactividad y selectividad con los grupos amino de prote\u00ednas.<\/p>\n","protected":false},"featured_media":8420,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8418"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8418\/revisions"}],"predecessor-version":[{"id":8427,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8418\/revisions\/8427"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8420"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8418"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8418"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}