{"id":8134,"date":"2023-03-10T17:47:45","date_gmt":"2023-03-10T23:47:45","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8134"},"modified":"2023-03-10T18:18:09","modified_gmt":"2023-03-11T00:18:09","slug":"readiuse-abts-substrate-solution","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/readiuse-abts-substrate-solution\/","title":{"rendered":"ReadiUse\u2122 ABTS Substrate Solution\u00a0"},"content":{"rendered":"\n

La peroxidasa de Horseradish (HRP) y los conjugados de HRP facilitan la oxidaci\u00f3n del ABTS en presencia de per\u00f3xido de hidr\u00f3geno, convirtiendo al ABTS en su producto oxidado azul verdoso. Esta reacci\u00f3n cromog\u00e9nica es ampliamente utilizada para cuantificar HRP en ensayos ELISA. <\/p>\n\n\n\n

El producto ABTS oxidado tiene un m\u00e1ximo de absorci\u00f3n de 420 nm que puede seguirse f\u00e1cilmente con un espectrofot\u00f3metro. La soluci\u00f3n ReadiUse\u2122 ABTS est\u00e1 optimizada para ensayos ELISA que utilizan HRP o conjugados marcados con HRP y per\u00f3xido de hidr\u00f3geno en placas de micropocillos o tubos de ensayo. <\/p>\n\n\n\n

Nuestra soluci\u00f3n ABTS permite que la reacci\u00f3n de HRP se realice en una sola adici\u00f3n. La soluci\u00f3n de ensayo cambia su color a verde claro al reaccionar con HRP o conjugados de HRP en presencia de per\u00f3xido de hidr\u00f3geno.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-11013<\/td>ReadiUse\u2122 ABTS Substrate Solution <\/td>100 ml<\/td><\/tr>
AAT-11001<\/td>ReadiUse\u2122 ABTS Substrate Solution <\/td>1 L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Refrigeraci\u00f3n a 2-8 \u00b0C. MInimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Importante<\/strong>: Permitir que la soluci\u00f3n ABTS ReadiUse\u2122 alcance temperatura ambiente antes de usarla. El reactivo debe usarse tal como se suministra, no se requiere diluci\u00f3n.<\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Absorbancia<\/mark><\/p>\n\n\n\n

Absorbancia<\/td>420 nm<\/td><\/tr>
Placa Recomendada<\/td>Fondo claro<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

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Figura 1. <\/strong>Estructura qu\u00edmica para ReadiUse\u2122 Soluci\u00f3n de sustrato ABTS (Optimizada para ensayos ELISA con conjugados HRP<\/em>)<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

ReadiUse\u2122 Preactivated APC-iFluor\u00ae 700 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-iFluor\u00ae 750 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 647 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 750 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 hydrogen peroxide solution *50 mM calibrated and stabilized solution*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated HRP NHS ester<\/a><\/td><\/tr>
ReadiUse\u2122 NADPH Regenerating Kit<\/a><\/td><\/tr>
ReadiUse\u2122 NADP Regenerating Kit<\/a><\/td><\/tr>
ReadiUse\u2122 TCA Deproteinization Sample Preparation Kit<\/a><\/td><\/tr>
ReadiUse\u2122 microscope mounting solution<\/a><\/td><\/tr>
ReadiUse\u2122 4% formaldehyde fixation solution<\/a><\/td><\/tr>
ReadiUse\u2122 mammalian cell lysis buffer *5X*<\/a><\/td><\/tr>
ReadiUse\u2122 probenecid, sodium salt *Water-soluble*<\/a><\/td><\/tr>
ReadiUse\u2122 probenecid *25 mM stabilized aqueous solution*<\/a><\/td><\/tr>
ReadiUse\u2122 CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy5.5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy7 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Texas Red Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-Cy5.5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-Cy7 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 TCEP removal solution<\/a><\/td><\/tr>
ReadiUse\u2122 WST-8 *50 mM aqueous solution*<\/a><\/td><\/tr>
ReadiUse\u2122 Rapid Luminometric ATP Assay Kit<\/a><\/td><\/tr>
ReadiUse\u2122 PE [R-Phycoerythrin] *Ammonium Sulfate-Free*<\/a><\/td><\/tr>
ReadiUse\u2122 CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 594 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Cell Detaching Buffer<\/a><\/td><\/tr>
ReadiUse\u2122 DRAQ5 Staining Solution *5 mM in Water*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 28 referencias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Plant extracts with anti-inflammatory properties–a new approach for characterization of their bioactive compounds and establishment of structure-antioxidant activity relationships
Authors: <\/strong>Amaral S, Mira L, Nogueira JM, da Silva AP, Helena Florencio M.
Journal: <\/strong>Bioorg Med Chem (2009): 1876<\/p>\n\n\n\n

A three-enzyme cascade reaction through positional assembly of enzymes in a polymersome nanoreactor
Authors: <\/strong>van Dongen SF, Nallani M, Cornelissen JJ, Nolte RJ, van Hest JC.
Journal: <\/strong>Chemistry (2009): 1107<\/p>\n\n\n\n

Degradation of glyphosate and other pesticides by ligninolytic enzymes
Authors: <\/strong>Pizzul L, Castillo MD, Stenstrom J.
Journal: <\/strong>Biodegradation. (2009)<\/p>\n\n\n\n

Self-assembly of enzymes on DNA scaffolds: en route to biocatalytic cascades and the synthesis of metallic nanowires
Authors: <\/strong>Wilner OI, Shimron S, Weizmann Y, Wang ZG, Willner I.
Journal: <\/strong>Nano Lett (2009): 2040<\/p>\n\n\n\n

Supramolecular cocaine-aptamer complexes activate biocatalytic cascades
Authors: <\/strong>Freeman R, Sharon E, Tel-Vered R, Willner I.
Journal: <\/strong>J Am Chem Soc (2009): 5028<\/p>\n\n\n\n

Cooperative multicomponent self-assembly of nucleic acid structures for the activation of DNAzyme cascades: a paradigm for DNA sensors and aptasensors
Authors: <\/strong>Elbaz J, Moshe M, Shlyahovsky B, Willner I.
Journal: <\/strong>Chemistry (2009): 3411<\/p>\n\n\n\n

Full-field photonic biosensors based on tunable bio-doped sol-gel glasses
Authors: <\/strong>Llobera A, Cadarso VJ, Darder M, Dominguez C, Fern and ez-Sanchez C., undefined
Journal: <\/strong>Lab Chip (2008): 1185<\/p>\n\n\n\n

Simultaneous total antioxidant capacity assay of lipophilic and hydrophilic antioxidants in the same acetone-water solution containing 2% methyl-beta-cyclodextrin using the cupric reducing antioxidant capacity (CUPRAC) method
Authors: <\/strong>Ozyurek M, Bektasoglu B, Guclu K, Gungor N, Apak R.
Journal: <\/strong>Anal Chim Acta (2008): 28<\/p>\n\n\n\n

Effects of mutations in the helix G region of horseradish peroxidase
Authors: <\/strong>Ryan BJ, O’Fagain C.
Journal: <\/strong>Biochimie (2008): 1414<\/p>\n\n\n\n

Glutamic acid-141: a heme ‘bodyguard’ in anionic tobacco peroxidase
Authors: <\/strong>Hushpulian DM, Poloznikov AA, Savitski PA, Rozhkova AM, Chubar TA, Fechina VA, Orlova MA, Tishkov VI, Gazaryan IG, Lagrimini LM.
Journal: <\/strong>Biol Chem (2007): 373<\/p>\n\n\n\n

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Application notes <\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
A Meta-Analysis of Common Calcium Indicators<\/a>
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a><\/p>\n\n\n\n

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FAQ<\/mark><\/p>\n\n\n\n

What are the detection methods for western blot?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a>
Are there any alternatives to Cy5?<\/a>
Are there any alternatives to indocyanine green (ICG)?<\/a>
Can DAPI bind to RNA?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise <\/mark><\/p>\n\n\n\n

Peroxidase Detection<\/a>
HRP Antibody Labeling Using Buccutite\u2122 Crosslinking Technology<\/a>
iFluor\u00ae 700 Dyes<\/a>
Hydroxyl Radical Detection<\/a>
Buccutite\u2122 Conjugation Kits: Quick and Easy Antibody Labeling<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

La soluci\u00f3n ReadiUse\u2122 ABTS est\u00e1 optimizada para ensayos ELISA que utilizan HRP o conjugados marcados con HRP y per\u00f3xido de hidr\u00f3geno en placas de micropocillos o tubos de ensayo. <\/p>\n","protected":false},"featured_media":8137,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8134"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8134\/revisions"}],"predecessor-version":[{"id":8141,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8134\/revisions\/8141"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8137"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8134"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8134"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}