{"id":8126,"date":"2023-03-10T16:49:05","date_gmt":"2023-03-10T22:49:05","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8126"},"modified":"2023-03-10T17:54:02","modified_gmt":"2023-03-10T23:54:02","slug":"amplite-adhp-10-acetyl-37-dihydroxyphenoxazine","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-adhp-10-acetyl-37-dihydroxyphenoxazine\/","title":{"rendered":"Amplite\u00ae ADHP [10-Acetyl-3,7-dihydroxyphenoxazine]"},"content":{"rendered":"\n

Nuestro Amplite\u00ae ADHP es qu\u00edmicamente igual a Amplex\u2122 Red (Amplex\u2122 Red es una marca comercial de Invitrogen). Es un sustrato de peroxidasa fluorog\u00e9nica sensible que tiene un fondo mucho m\u00e1s bajo que el material de otros proveedores comerciales. ADHP genera resorufina altamente fluorescente que tiene una absorci\u00f3n m\u00e1xima de 571 nm y una emisi\u00f3n m\u00e1xima de 585 nm. <\/p>\n\n\n\n

A diferencia de otros sustratos de HRP, como las dihidrofluoresce\u00ednas y las dihidrorodaminas, la oxidaci\u00f3n por aire del ADHP es m\u00ednima. Hasta ahora, ADHP ha sido conocida como la sonda fluorog\u00e9nica m\u00e1s sensible y estable para detectar HRP y H2O2. ADHP se ha utilizado ampliamente para detectar HRP en muchos inmunoensayos. <\/p>\n\n\n\n

Por otro lado, ADHP tambi\u00e9n se puede utilizar para detectar trazas de H2O2. La detecci\u00f3n de H2O2 basada en ADHP es al menos un orden de magnitud m\u00e1s sensible que el ensayo de escopoletina com\u00fanmente usado para H2O2. Debido a que el H2O2 se produce en muchas reacciones enzim\u00e1ticas redox, el ADHP puede usarse en reacciones enzim\u00e1ticas acopladas para detectar la actividad de muchas oxidasas y\/o enzimas\/sustratos o cofactores relacionados, como glucosa, acetilcolina y colesterol, L-glutamato, amino\u00e1cidos, etc. .<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-11000<\/td>Amplite\u00ae ADHP [10-Acetyl-3,7-dihydroxyphenoxazine]<\/td>25 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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SDS<\/a><\/p>\n\n\n\n

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Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n a <-15\u00a0\u00b0C. MInimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Espectro<\/mark><\/p>\n\n\n\n

Abir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>571<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>584<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/strong>
Volumen del solvente apropiado necesario para reconstituir la masa espec\u00edfica de Amplite\u00ae ADHP [10-acetil-3,7-dihidroxifenoxazina] CAS#: 119171-73-2<\/em> a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>388.742 \u00b5L<\/td>1.944 mL<\/td>3.887 mL<\/td>19.437 mL<\/td>38.874 mL<\/td><\/tr>
5 mM<\/strong><\/td>77.748 \u00b5L<\/td>388.742 \u00b5L<\/td>777.484 \u00b5L<\/td>3.887 mL<\/td>7.775 mL<\/td><\/tr>
10 mM<\/strong><\/td>38.874 \u00b5L<\/td>194.371 \u00b5L<\/td>388.742 \u00b5L<\/td>1.944 mL<\/td>3.887 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1. <\/strong>Estructura quimica para Amplite\u00ae ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*<\/p>\n\n\n\n

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Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2.<\/strong> La radiaci\u00f3n aumenta la actividad enzim\u00e1tica de MPO y los leucocitos portadores de MPO en el microambiente tumoral. (A) Gr\u00e1ficos de barras que muestran un aumento de la actividad enzim\u00e1tica de la MPO medida como unidades relativas de fluorescencia (RFU)\/seg\/mg de prote\u00edna en tumores irradiados (IR) en comparaci\u00f3n con tumores no irradiados (sin IR). CL cerebro: hemisferio cerebral normal contralateral desprovisto de tumor. n = 4\/grupo (B) Gr\u00e1ficos de puntos de citometr\u00eda de flujo que muestran neutr\u00f3filos y ant\u00edgeno linfocitario 6C (Ly6C) con alto contenido de monocitos\/macr\u00f3fagos (Mo\/M\u03a6) en tumores irradiados (IR) y no irradiados (sin IR). SSC: dispersi\u00f3n lateral, Ly6G: ant\u00edgeno linfocitario 6G. (C) An\u00e1lisis de citometr\u00eda de flujo que muestra el n\u00famero de c\u00e9lulas de monocitos\/macr\u00f3fagos (Mo\/M\u03a6) o monocitos (Mo) de neutr\u00f3filos y Ly-6Chigh del total de leucocitos definidos como c\u00e9lulas CD45+. Se aislaron c\u00e9lulas de tumor cerebral (izquierda), sangre perif\u00e9rica (centro) y m\u00e9dula \u00f3sea (derecha) de ratones irradiados (IR) y no irradiados (sin IR) (n = 4-5\/grupo). Los ratones se irradiaron 14 d\u00edas despu\u00e9s de las inyecciones en el tumor y se realiz\u00f3 una citometr\u00eda de flujo 1 semana despu\u00e9s de la radiaci\u00f3n. La actividad de MPO de la MPO capturada se evalu\u00f3 con 10-acetil-3,7-dihidroxifenoxazina (ADHP, AAT Bioquest). Datos representados como media \u00b1 SEM. * p < 0,05. Fuente: La mieloperoxidasa ejerce actividad antitumoral en el glioma despu\u00e9s de la radioterapia por Ali et. al., Neoplasia. Abril 2022.<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td><\/tr>
Amplite\u00ae IR<\/a><\/td>648<\/td>668<\/td><\/tr>
Amplite\u00ae Red<\/a><\/td>571<\/td>584<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 20 bibliograf\u00edas: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy<\/a>
Authors: <\/strong>Ali, Muhammad and Fulci, Giulia and Grigalavicius, Mantas and Pulli, Benjamin and Li, Anning and Wojtkiewicz, Gregory R and Wang, Cuihua and Hsieh, Kevin Li-Chun and Linnoila, Jenny J and Theodossiou, Theodossis A and others,
Journal: <\/strong>Neoplasia (2022): 100779<\/p>\n\n\n\n

Myeloperoxidase modulates inflammation in generalized pustular psoriasis and additional rare pustular skin diseases<\/a>
Authors: <\/strong>Haskamp, Stefan and Bruns, Heiko and Hahn, Madelaine and Hoffmann, Markus and Gregor, Anne and L{\\”o}hr, Sabine and Hahn, Jonas and Schauer, Christine and Ringer, Mark and Flamann, Cindy and others,
Journal: <\/strong>The American Journal of Human Genetics (2020): 527–538<\/p>\n\n\n\n

C3 opsonization of anthrax bacterium and peptidoglycan supports recognition and activation of neutrophils<\/a>
Authors: <\/strong>Popescu, Narcis I and Keshari, Ravi S and Cochran, Jackie and Coggeshall, K Mark and Lupu, Florea
Journal: <\/strong>Microorganisms (2020): 1039<\/p>\n\n\n\n

Multimodal Molecular Imaging Demonstrates Myeloperoxidase Regulation of Matrix Metalloproteinase Activity in Neuroinflammation<\/a>
Authors: <\/strong>Zhang, Yinian and Dong, Huateng and Seeburg, Daniel P and Wojtkiewicz, Gregory R and Waterman, Peter and Pulli, Benjamin and Forghani, Reza and Ali, Muhammad and Iwamoto, Yoshiko and Swirski, Filip K and others, undefined
Journal: <\/strong>Molecular neurobiology (2018): 1–9<\/p>\n\n\n\n

Spinal cord inflammation: molecular imaging after thoracic aortic ischemia reperfusion injury
Authors: <\/strong>Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: <\/strong>Radiology (2017): 202–211<\/p>\n\n\n\n

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase<\/a>
Authors: <\/strong>Milton, Amber
Journal: <\/strong>(2017)<\/p>\n\n\n\n

Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA<\/a>
Authors: <\/strong>Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: <\/strong>Analytical Chemistry (2017)<\/p>\n\n\n\n

Patterned photonic nitrocellulose for pseudopaper ELISA<\/a>
Authors: <\/strong>Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: <\/strong>Analytical chemistry (2017): 7727–7733<\/p>\n\n\n\n

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase<\/a>
Authors: <\/strong>Milton, Amber
Journal: <\/strong>(2017)<\/p>\n\n\n\n

Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury<\/a>
Authors: <\/strong>Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: <\/strong>Radiology (2016): 152222<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 61 referencias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Volatile fatty acid-sensing system involving coenzyme-A transferase
Authors: <\/strong>Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.
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Galactose oxidase action on galactose containing glycolipids–a fluorescence method
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Effects of chronic ethanol administration on the activities and relative synthetic rates of myelin and synaptosomal plasma membrane-associated sialidase in the rat brain
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Mitochondrial reactive oxygen species in mice lacking superoxide dismutase 2: attenuation via antioxidant treatment
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A new, sensitive method for enzyme kinetic studies of scarce glucosides
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Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species
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Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
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A Role for Mitochondrial Dysfunction in Perpetuating Radiation-Induced Genomic Instability
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Sexual Dimorphism in Oxidant Status in Spontaneously Hypertensive Rats
Authors: <\/strong>Sullivan JC, Sasser JM, Pollock JS.
Journal: <\/strong>Am J Physiol Regul Integr Comp Physiol. (2006)<\/p>\n\n\n\n

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Application notes <\/mark><\/p>\n\n\n\n

A Library of Well-Defined and Water-Soluble Poly(alkyl phosphonate)s with Adjustable Hydrolysis<\/a>
Acetylcholinesterase Inhibitory Activity of Pigment Echinochrome A<\/a>
Ameliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer’s Disease<\/a>
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults<\/a>
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity<\/a><\/a><\/p>\n\n\n\n

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FAQ<\/mark><\/p>\n\n\n\n

Are coumarins water-soluble?<\/a>
Are NADH and ROS related?<\/a>
Can I measure NADPH without lysing my cells?<\/a>
Can I use Amplite Fluorimetric Glutamic Acid Assay Kit with an absorbance microplate reader?<\/a>
How are fatty acids activated?<\/a><\/p>\n\n\n\n

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AssayWise <\/mark><\/p>\n\n\n\n

Peroxidase Detection<\/a>
Acetylcholinesterase Quantification<\/a>
Acetylcholinesterase Assays<\/a>
Glycerol 3-Phosphate Assays<\/a>
Catalase Detection<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

ADHP se ha utilizado ampliamente y se conoce como la sonda fluorog\u00e9nica m\u00e1s sensible y estable para detectar HRP y H2O2 en muchos inmunoensayos. <\/p>\n","protected":false},"featured_media":8132,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8126"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":4,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8126\/revisions"}],"predecessor-version":[{"id":8135,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8126\/revisions\/8135"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8132"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8126"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8126"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}