{"id":8116,"date":"2023-03-10T15:53:54","date_gmt":"2023-03-10T21:53:54","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8116"},"modified":"2023-03-10T16:32:06","modified_gmt":"2023-03-10T22:32:06","slug":"amplite-blue","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-blue\/","title":{"rendered":"Amplite\u00ae Blue"},"content":{"rendered":"\n

Hay una variedad de sustratos disponibles para detectar la actividad de la peroxidasa en los ensayos ELISA. <\/p>\n\n\n\n

Los sustratos colorim\u00e9tricos de HRP (p. ej., TMB, OPD y ABTS) se han utilizado ampliamente durante a\u00f1os. Cada uno de estos sustratos var\u00eda mucho con respecto a sus caracter\u00edsticas de rendimiento, como la sensibilidad de detecci\u00f3n, el rango de trabajo y las relaciones se\u00f1al\/ruido alcanzables. <\/p>\n\n\n\n

Nuestro sustrato de peroxidasa Amplite\u00ae Blue es un sustrato fluorog\u00e9nico soluble para la detecci\u00f3n de la actividad de la peroxidasa. Amplite\u00ae Blue permite realizar ensayos de detecci\u00f3n r\u00e1pida de HRP con mayor sensibilidad que los sustratos colorim\u00e9tricos de HRP (como TMB y OPD). El producto fluorescente de Amplite\u00ae Blue no fotoblanquea. Amplite\u00ae Blue exhibe una l\u00ednea de base plana en los ensayos, lo que facilita la sensibilidad de detecci\u00f3n de bajo nivel y permite altas relaciones se\u00f1al-ruido.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-11005<\/td>Amplite\u00ae Blue AAT Bioquest<\/td>25 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n a <-15 \u00b0C. Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>324 nm<\/td><\/tr>
Emisi\u00f3n<\/td>409 nm<\/td><\/tr>
Cuttoff<\/td>350 nm<\/td><\/tr>
Placa recomendada <\/td>Placa negra<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Notas importantes<\/strong>
Es importante almacenar a <-15 \u00b0C y debe almacenarse en un lugar fresco y oscuro.<\/p>\n\n\n\n

Se puede utilizar en un plazo de 12 meses a partir de la fecha de recepci\u00f3n.<\/p>\n<\/div><\/div>\n\n\n\n

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Imagen<\/mark><\/p>\n\n\n\n

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Figura 1. <\/strong>La respuesta a la dosis de HRP se midi\u00f3 con el kit de ensayo de peroxidasa fluorim\u00e9trica Amplite\u00ae en una placa negra utilizando un lector de microplacas de fluorescencia Gemini (Molecular Devices).<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Amplite\u00ae Red<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase<\/a>
Authors: <\/strong>Milton, Amber
Journal: <\/strong>(2017)<\/p>\n\n\n\n

Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA<\/a>
Authors: <\/strong>Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: <\/strong>Analytical Chemistry (2017)<\/p>\n\n\n\n

Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury<\/a>
Authors: <\/strong>Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: <\/strong>Radiology (2016): 152222<\/p>\n\n\n\n

Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis<\/a>
Authors: <\/strong>Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W
Journal: <\/strong>Antioxidants &amp; redox signaling (2015): 1255–1269<\/p>\n\n\n\n

Myeloperoxidase Nuclear Imaging for Epileptogenesis<\/a>
Authors: <\/strong>Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others, undefined
Journal: <\/strong>Radiology (2015): 822–830<\/p>\n\n\n\n

Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs<\/a>
Authors: <\/strong>Huang, Jiansheng and Smith, Forrest and Panizzi, Peter
Journal: <\/strong>Archives of biochemistry and biophysics (2014): 74–85<\/p>\n\n\n\n

Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings<\/a>
Authors: <\/strong>Scherag, Frank D and Br, undefined and stetter, Thomas and R&uuml;he, J&uuml;rgen
Journal: <\/strong>Colloids and Surfaces B: Biointerfaces (2014): 576–582<\/p>\n\n\n\n

Measuring myeloperoxidase activity in biological samples<\/a>
Authors: <\/strong>Pulli, Benjamin and Ali, Muhammad and Forghani, Reza and Schob, Stefan and Hsieh, Kevin LC and Wojtkiewicz, Gregory and Linnoila, Jenny J and Chen, John W
Journal: <\/strong>PLoS One (2013): e67976<\/p>\n\n\n\n

Micro-volume wall-less immunoassays using patterned planar plates<\/a>
Authors: <\/strong>Kozak, Katherine R and Wang, Jianyong and Lye, Melvin and Takkar, Rashi and Kim, Namyong and Lee, Hyunjae and Jeon, Noo Li and Lin, Kedan and Zhang, Crystal and Wong, Wai Lee T and others, undefined
Journal: <\/strong>Lab on a Chip (2013): 1342–1350<\/p>\n\n\n\n

Distinguishing inflammation from tumor and peritumoral edema by myeloperoxidase magnetic resonance imaging<\/a>
Authors: <\/strong>Kleijn, Anne and Chen, John W and Buhrman, Jason S and Wojtkiewicz, Gregory R and Iwamoto, Yoshiko and Lamfers, Martine L and Stemmer-Rachamimov, Anat O and Rabkin, Samuel D and Weissleder, Ralph and Martuza, Robert L and others, undefined
Journal: <\/strong>Clinical Cancer Research (2011): 4484–4493<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 61 referencias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Volatile fatty acid-sensing system involving coenzyme-A transferase
Authors: <\/strong>Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.
Journal: <\/strong>Biotechnol Prog (2006): 334<\/p>\n\n\n\n

Galactose oxidase action on galactose containing glycolipids–a fluorescence method
Authors: <\/strong>Fortelius M, Mattjus P.
Journal: <\/strong>Chem Phys Lipids (2006): 103<\/p>\n\n\n\n

Effects of chronic ethanol administration on the activities and relative synthetic rates of myelin and synaptosomal plasma membrane-associated sialidase in the rat brain
Authors: <\/strong>Azuine MA, Patel SJ, Lakshman MR.
Journal: <\/strong>Neurochem Int (2006): 67<\/p>\n\n\n\n

Mitochondrial reactive oxygen species in mice lacking superoxide dismutase 2: attenuation via antioxidant treatment
Authors: <\/strong>Morten KJ, Ackrell BA, Melov S.
Journal: <\/strong>J Biol Chem (2006): 3354<\/p>\n\n\n\n

A new, sensitive method for enzyme kinetic studies of scarce glucosides
Authors: <\/strong>Mazura P, Fohlerova R, Brzobohaty B, Kiran NS, J and a L., undefined
Journal: <\/strong>J Biochem Biophys Methods (2006): 55<\/p>\n\n\n\n

Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species
Authors: <\/strong>Rinaldi M, Moroni P, Paape MJ, Bannerman DD.
Journal: <\/strong>Vet Immunol Immunopathol. (2006)<\/p>\n\n\n\n

Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
Authors: <\/strong>Fruk L, Muller J, Niemeyer CM.
Journal: <\/strong>Chemistry (2006): 7448<\/p>\n\n\n\n

K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling
Authors: <\/strong>Drose S, Br and t U, Hanley PJ.
Journal: <\/strong>J Biol Chem (2006): 23733<\/p>\n\n\n\n

A Role for Mitochondrial Dysfunction in Perpetuating Radiation-Induced Genomic Instability
Authors: <\/strong>Kim GJ, Fiskum GM, Morgan WF.
Journal: <\/strong>Cancer Res (2006): 10377<\/p>\n\n\n\n

Sexual Dimorphism in Oxidant Status in Spontaneously Hypertensive Rats
Authors: <\/strong>Sullivan JC, Sasser JM, Pollock JS.
Journal: <\/strong>Am J Physiol Regul Integr Comp Physiol. (2006)<\/p>\n\n\n\n

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Application notes <\/mark><\/p>\n\n\n\n

A Library of Well-Defined and Water-Soluble Poly(alkyl phosphonate)s with Adjustable Hydrolysis<\/a>
Acetylcholinesterase Inhibitory Activity of Pigment Echinochrome A<\/a>
Ameliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer’s Disease<\/a>
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults<\/a>
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity<\/a><\/p>\n\n\n\n

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FAQ<\/mark><\/p>\n\n\n\n

What are the detection methods for western blot?<\/a>
Are coumarins water-soluble?<\/a>
Are NADH and ROS related?<\/a>
Can I measure NADPH without lysing my cells?<\/a>
Can I use Amplite Fluorimetric Glutamic Acid Assay Kit with an absorbance microplate reader?<\/a><\/p>\n\n\n\n

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AssayWise <\/mark><\/p>\n\n\n\n

Acetylcholinesterase Quantification<\/a>
Acetylcholinesterase Assays<\/a>
Glycerol 3-Phosphate Assays<\/a>
Catalase Detection<\/a>
Hydrogen Peroxide Detection<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Amplite\u00ae Blue permite realizar ensayos de detecci\u00f3n r\u00e1pida de HRP con mayor sensibilidad que los sustratos colorim\u00e9tricos de HRP (como TMB y OPD). <\/p>\n","protected":false},"featured_media":8117,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8116"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":5,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8116\/revisions"}],"predecessor-version":[{"id":8124,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8116\/revisions\/8124"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8117"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8116"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8116"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}