{"id":8111,"date":"2023-03-10T15:30:43","date_gmt":"2023-03-10T21:30:43","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8111"},"modified":"2023-03-10T15:49:56","modified_gmt":"2023-03-10T21:49:56","slug":"amplite-ir","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-ir\/","title":{"rendered":"Amplite\u00ae IR"},"content":{"rendered":"\n

Nuestro Amplite\u00ae IR es un sustrato de peroxidasa fluorog\u00e9nica que genera fluorescencia infrarroja cercana al reaccionar con peroxidasa y H2O2. Se puede utilizar para detectar tanto H2O2 como peroxidasa. <\/p>\n\n\n\n

Amplite\u00ae IR genera una sustancia que tiene una absorci\u00f3n m\u00e1xima de 647 nm con una emisi\u00f3n m\u00e1xima de 670 nm. Esta absorci\u00f3n y fluorescencia del infrarrojo cercano minimizan el fondo del ensayo que a menudo es causado por la autoabsorci\u00f3n y\/o la autofluorescencia de muestras biol\u00f3gicas que rara vez absorben luz m\u00e1s all\u00e1 de 600 nm. A diferencia de otros sustratos de HRP, como las dihidrofluoresce\u00ednas y las dihidrorodaminas, la oxidaci\u00f3n por aire de Amplite\u00ae IR es m\u00ednima. <\/p>\n\n\n\n

En comparaci\u00f3n con Ampplex Red\u2122, Amplite\u00ae IR genera una fluorescencia que es independiente del pH entre pH 4 y 10. Adem\u00e1s, tiene una excelente solubilidad en agua. Es una alternativa superior a Amplex Red\u2122 para las detecciones que requieren un pH bajo donde Amplex Red\u2122 tiene una fluorescencia significativamente reducida. Hemos utilizado Amplite\u00ae IR para detectar HRP en bastantes inmunoensayos. <\/p>\n\n\n\n

Amplite\u00ae IR tambi\u00e9n se puede utilizar para detectar trazas de H2O2. Dado que el H2O2 se produce en muchas reacciones enzim\u00e1ticas redox, Amplite\u00ae IR se puede utilizar en reacciones enzim\u00e1ticas acopladas para detectar la actividad de muchas oxidasas y\/o enzimas\/sustratos o cofactores relacionados, como glucosa, acetilcolina y colesterol, L-glutamato, amino\u00e1cidos etc.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-11009<\/td>Amplite\u00ae IT, AAT Bioquest<\/td>1 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n a <-15 \u00b0C. Minimizar la exposici\u00f3n a la luz, disecado.<\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>640 nm<\/td><\/tr>
Emisi\u00f3n<\/td>680 nm<\/td><\/tr>
Cuttoff<\/td>650 nm<\/td><\/tr>
Placa recomendada <\/td>Placa negra<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en\u00a0Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>648<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>668<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n de Soluciones de Stock<\/strong><\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

Soluci\u00f3n madre de Amplite IR :<\/em>
Agregue la cantidad apropiada de DMSO anhidro para preparar una soluci\u00f3n madre Amplite IR de 10 a 25 mM.<\/p>\n\n\n\n

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Preparaci\u00f3n de Soluciones de Trabajo<\/strong><\/p>\n\n\n\n

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Soluci\u00f3n de trabajo Amplite IR (2X):<\/em>
Para lograr una concentraci\u00f3n final por pocillo de 50 a 100 \u00b5M en buffer de fosfato de 50 mM o el buffer de su elecci\u00f3n, prepare una soluci\u00f3n de concentraci\u00f3n de 100 a 200 \u00b5M en un tubo. Se requieren 50 \u00b5L por pocillo. Nota:<\/strong> Amplite IR es inestable en presencia de tioles como DTT y b-mercaptoetanol. Los tioles superiores a 10 \u03bcM (concentraci\u00f3n final) podr\u00edan disminuir significativamente el rango din\u00e1mico del ensayo. El NADH y el glutati\u00f3n (reducido de: GSH) pueden interferir con el ensayo. Nota<\/strong>: Recomendamos utilizar una soluci\u00f3n madre nueva cada vez que realice experimentos.<\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

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Imagenes<\/mark><\/p>\n\n\n\n

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Figura 1. <\/strong>La respuesta a la dosis de H2O2 se midi\u00f3 en una placa negra s\u00f3lida de 96 pocillos con el kit de ensayo de per\u00f3xido de hidr\u00f3geno fluorim\u00e9trico Amplite\u00ae.<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Amplite\u00ae Red<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Wearable materials with embedded synthetic biology sensors for biomolecule detection<\/a>
Authors:\u00a0<\/strong>Nguyen, Peter Q and Soenksen, Luis R and Donghia, Nina M and Angenent-Mari, Nicolaas M and de Puig, Helena and Huang, Ally and Lee, Rose and Slomovic, Shimyn and Galbersanini, Tommaso and Lansberry, Geoffrey and others,
Journal:\u00a0<\/strong>Nature Biotechnology\u00a0(2021):\u00a01366–1374<\/p>\n\n\n\n

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase<\/a>
Authors: <\/strong>Milton, Amber
Journal: <\/strong>(2017)<\/p>\n\n\n\n

Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA<\/a>
Authors: <\/strong>Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: <\/strong>Analytical Chemistry (2017)<\/p>\n\n\n\n

Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury<\/a>
Authors: <\/strong>Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: <\/strong>Radiology (2016): 152222<\/p>\n\n\n\n

Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis<\/a>
Authors: <\/strong>Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W
Journal: <\/strong>Antioxidants &amp; redox signaling (2015): 1255–1269<\/p>\n\n\n\n

Myeloperoxidase Nuclear Imaging for Epileptogenesis<\/a>
Authors: <\/strong>Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others, undefined
Journal: <\/strong>Radiology (2015): 822–830<\/p>\n\n\n\n

Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs<\/a>
Authors: <\/strong>Huang, Jiansheng and Smith, Forrest and Panizzi, Peter
Journal: <\/strong>Archives of biochemistry and biophysics (2014): 74–85<\/p>\n\n\n\n

Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings<\/a>
Authors: <\/strong>Scherag, Frank D and Br, undefined and stetter, Thomas and R&uuml;he, J&uuml;rgen
Journal: <\/strong>Colloids and Surfaces B: Biointerfaces (2014): 576–582<\/p>\n\n\n\n

Measuring myeloperoxidase activity in biological samples<\/a>
Authors: <\/strong>Pulli, Benjamin and Ali, Muhammad and Forghani, Reza and Schob, Stefan and Hsieh, Kevin LC and Wojtkiewicz, Gregory and Linnoila, Jenny J and Chen, John W
Journal: <\/strong>PLoS One (2013): e67976<\/p>\n\n\n\n

Micro-volume wall-less immunoassays using patterned planar plates<\/a>
Authors: <\/strong>Kozak, Katherine R and Wang, Jianyong and Lye, Melvin and Takkar, Rashi and Kim, Namyong and Lee, Hyunjae and Jeon, Noo Li and Lin, Kedan and Zhang, Crystal and Wong, Wai Lee T and others, undefined
Journal: <\/strong>Lab on a Chip (2013): 1342–1350<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 61 referencias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Volatile fatty acid-sensing system involving coenzyme-A transferase
Authors: <\/strong>Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.
Journal: <\/strong>Biotechnol Prog (2006): 334<\/p>\n\n\n\n

Galactose oxidase action on galactose containing glycolipids–a fluorescence method
Authors: <\/strong>Fortelius M, Mattjus P.
Journal: <\/strong>Chem Phys Lipids (2006): 103<\/p>\n\n\n\n

Effects of chronic ethanol administration on the activities and relative synthetic rates of myelin and synaptosomal plasma membrane-associated sialidase in the rat brain
Authors: <\/strong>Azuine MA, Patel SJ, Lakshman MR.
Journal: <\/strong>Neurochem Int (2006): 67<\/p>\n\n\n\n

Mitochondrial reactive oxygen species in mice lacking superoxide dismutase 2: attenuation via antioxidant treatment
Authors: <\/strong>Morten KJ, Ackrell BA, Melov S.
Journal: <\/strong>J Biol Chem (2006): 3354<\/p>\n\n\n\n

A new, sensitive method for enzyme kinetic studies of scarce glucosides
Authors: <\/strong>Mazura P, Fohlerova R, Brzobohaty B, Kiran NS, J and a L., undefined
Journal: <\/strong>J Biochem Biophys Methods (2006): 55<\/p>\n\n\n\n

Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species
Authors: <\/strong>Rinaldi M, Moroni P, Paape MJ, Bannerman DD.
Journal: <\/strong>Vet Immunol Immunopathol. (2006)<\/p>\n\n\n\n

Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
Authors: <\/strong>Fruk L, Muller J, Niemeyer CM.
Journal: <\/strong>Chemistry (2006): 7448<\/p>\n\n\n\n

K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling
Authors: <\/strong>Drose S, Br and t U, Hanley PJ.
Journal: <\/strong>J Biol Chem (2006): 23733<\/p>\n\n\n\n

A Role for Mitochondrial Dysfunction in Perpetuating Radiation-Induced Genomic Instability
Authors: <\/strong>Kim GJ, Fiskum GM, Morgan WF.
Journal: <\/strong>Cancer Res (2006): 10377<\/p>\n\n\n\n

Sexual Dimorphism in Oxidant Status in Spontaneously Hypertensive Rats
Authors: <\/strong>Sullivan JC, Sasser JM, Pollock JS.
Journal: <\/strong>Am J Physiol Regul Integr Comp Physiol. (2006)<\/p>\n\n\n\n

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Application notes <\/mark><\/p>\n\n\n\n

A Library of Well-Defined and Water-Soluble Poly(alkyl phosphonate)s with Adjustable Hydrolysis<\/a>
Acetylcholinesterase Inhibitory Activity of Pigment Echinochrome A<\/a>
Ameliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer’s Disease<\/a>
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults<\/a>
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity<\/a><\/p>\n\n\n\n

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FAQ<\/mark><\/p>\n\n\n\n

Are coumarins water-soluble?<\/a>
Are NADH and ROS related?<\/a>
Can I measure NADPH without lysing my cells?<\/a>
Can I use Amplite Fluorimetric Glutamic Acid Assay Kit with an absorbance microplate reader?<\/a>
How are fatty acids activated?<\/a><\/p>\n\n\n\n

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AssayWise <\/mark><\/p>\n\n\n\n

Acetylcholinesterase Quantification<\/a>
Acetylcholinesterase Assays<\/a>
Glycerol 3-Phosphate Assays<\/a>
Catalase Detection<\/a>
Hydrogen Peroxide Detection<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Amplite\u00ae IR se puede utilizar en reacciones enzim\u00e1ticas acopladas para detectar la actividad de muchas oxidasas y\/o enzimas\/sustratos o cofactores relacionados, como glucosa, acetilcolina y colesterol, L-glutamato, amino\u00e1cidos etc.<\/p>\n","protected":false},"featured_media":8113,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8111"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8111\/revisions"}],"predecessor-version":[{"id":8115,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8111\/revisions\/8115"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8113"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8111"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8111"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}