{"id":8105,"date":"2023-03-10T10:37:46","date_gmt":"2023-03-10T16:37:46","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8105"},"modified":"2023-03-10T16:33:55","modified_gmt":"2023-03-10T22:33:55","slug":"amplite-red","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-red\/","title":{"rendered":"Amplite\u00ae Red"},"content":{"rendered":"\n

Nuestro Amplite\u00ae Red es un sustrato de peroxidasa fluorog\u00e9nica sensible que genera un producto fluorescente muy rojo que tiene una absorci\u00f3n m\u00e1xima de 571 nm y una emisi\u00f3n m\u00e1xima de 585 nm. <\/p>\n\n\n\n

A diferencia de otros sustratos de HRP, como las dihidrofluoresce\u00ednas y las dihidrorodaminas, la oxidaci\u00f3n por aire de Amplite\u00ae Red es m\u00ednima. Amplite\u00ae Red es una de las sondas fluorog\u00e9nicas m\u00e1s sensibles y estables para detectar HRP y H2O2. Amplite\u00ae Red se ha utilizado ampliamente para detectar HRP en muchos inmunoensayos. Por otro lado, Amplite\u00ae Red tambi\u00e9n se puede utilizar para detectar trazas de H2O2. La detecci\u00f3n de H2O2 basada en Amplite\u00ae Red es al menos un orden de magnitud m\u00e1s sensible que el ensayo de escopoletina com\u00fanmente usado para H2O2. <\/p>\n\n\n\n

Debido a que el H2O2 se produce en muchas reacciones enzim\u00e1ticas redox, Amplite\u00ae Red puede usarse en reacciones enzim\u00e1ticas acopladas para detectar la actividad de muchas oxidasas y\/o enzimas\/sustratos o cofactores relacionados, como glucosa, acetilcolina y colesterol, L-glutamato, amino\u00e1cidos , etc.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-11011<\/td>Amplite\u00ae Red AAT Bioquest<\/td>1000 Ensayos<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n a <-15 \u00b0C. Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>540 nm<\/td><\/tr>
Emisi\u00f3n<\/td>590 nm<\/td><\/tr>
Cuttoff<\/td>550 nm<\/td><\/tr>
Placa recomendada <\/td>Placa negra<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>571<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>584<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n de Soluciones de Stock<\/strong><\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

Soluci\u00f3n madre de AmpliteTM Red (250X):<\/em>
Agregue 200 ml de DMSO anhidro en el vial y mezcle bien. La soluci\u00f3n madre debe usarse con prontitud. Cualquier soluci\u00f3n no utilizada debe dividirse en al\u00edcuotas y volver a congelarse a < -20 o<\/sup>C. Nota: Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n y prot\u00e9jalo de la luz.<\/p>\n\n\n\n

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Preparaci\u00f3n de Soluciones de Trabajo<\/strong><\/p>\n\n\n\n

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  1. Soluci\u00f3n de trabajo de peroxidasa roja AmpliteTM (1X):<\/em>
    Agregue 20 \u03bcL de soluci\u00f3n madre AmpliteTM Red (250X) en 5 mL de buffer de fosfato 50 mM o el buffer de su elecci\u00f3n, pH 7 con H2O2 200 mM. Nota: <\/strong>AmpliteTM Red es inestable en presencia de tioles como DTT y b-mercaptoetanol. Los tioles superiores a 10 \u03bcM (concentraci\u00f3n final) podr\u00edan disminuir significativamente el rango din\u00e1mico del ensayo. El NADH y el glutati\u00f3n (reducido de: GSH) pueden interferir con el ensayo<\/li>
  2. Soluci\u00f3n de trabajo AmpliteTM Red H2O2 (1X):<\/em>
    Agregue 20 \u03bcL de soluci\u00f3n madre AmpliteTM Red (250X) en 5 mL de buffer de fosfato 50 mM o el buffer de su elecci\u00f3n, pH 7 con 0,4 unidades\/mL de peroxidasa. Nota:<\/strong> AmpliteTM Red es inestable en presencia de tioles como DTT y b-mercaptoetanol. Los tioles superiores a 10 \u03bcM (concentraci\u00f3n final) podr\u00edan disminuir significativamente el rango din\u00e1mico del ensayo. El NADH y el glutati\u00f3n (reducido de: GSH) pueden interferir con el ensayo.<\/li><\/ol>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n
    <\/div>\n\n\n\n

    Imagenes<\/mark><\/p>\n\n\n\n

    <\/div>\n\n\n\n
    \"\"<\/figure>\n\n\n\n

    Figura 1. <\/strong>La respuesta a la dosis de HRP se midi\u00f3 con el kit de ensayo de peroxidasa fluorim\u00e9trica Amplite\u00ae en una placa negra utilizando un lector de microplacas de fluorescencia Gemini (Molecular Devices).<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Productos Relacionados<\/mark><\/p>\n\n\n\n

    Amplite\u00ae Blue<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

    Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase<\/a>
    Authors: <\/strong>Milton, Amber
    Journal: <\/strong>(2017)<\/p>\n\n\n\n

    Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA<\/a>
    Authors: <\/strong>Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
    Journal: <\/strong>Analytical Chemistry (2017)<\/p>\n\n\n\n

    Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury<\/a>
    Authors: <\/strong>Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
    Journal: <\/strong>Radiology (2016): 152222<\/p>\n\n\n\n

    Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis<\/a>
    Authors: <\/strong>Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W
    Journal: <\/strong>Antioxidants &amp; redox signaling (2015): 1255–1269<\/p>\n\n\n\n

    Myeloperoxidase Nuclear Imaging for Epileptogenesis<\/a>
    Authors: <\/strong>Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others, undefined
    Journal: <\/strong>Radiology (2015): 822–830<\/p>\n\n\n\n

    Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs<\/a>
    Authors: <\/strong>Huang, Jiansheng and Smith, Forrest and Panizzi, Peter
    Journal: <\/strong>Archives of biochemistry and biophysics (2014): 74–85<\/p>\n\n\n\n

    Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings<\/a>
    Authors: <\/strong>Scherag, Frank D and Br, undefined and stetter, Thomas and R&uuml;he, J&uuml;rgen
    Journal: <\/strong>Colloids and Surfaces B: Biointerfaces (2014): 576–582<\/p>\n\n\n\n

    Measuring myeloperoxidase activity in biological samples<\/a>
    Authors: <\/strong>Pulli, Benjamin and Ali, Muhammad and Forghani, Reza and Schob, Stefan and Hsieh, Kevin LC and Wojtkiewicz, Gregory and Linnoila, Jenny J and Chen, John W
    Journal: <\/strong>PLoS One (2013): e67976<\/p>\n\n\n\n

    Micro-volume wall-less immunoassays using patterned planar plates<\/a>
    Authors: <\/strong>Kozak, Katherine R and Wang, Jianyong and Lye, Melvin and Takkar, Rashi and Kim, Namyong and Lee, Hyunjae and Jeon, Noo Li and Lin, Kedan and Zhang, Crystal and Wong, Wai Lee T and others, undefined
    Journal: <\/strong>Lab on a Chip (2013): 1342–1350<\/p>\n\n\n\n

    Distinguishing inflammation from tumor and peritumoral edema by myeloperoxidase magnetic resonance imaging<\/a>
    Authors: <\/strong>Kleijn, Anne and Chen, John W and Buhrman, Jason S and Wojtkiewicz, Gregory R and Iwamoto, Yoshiko and Lamfers, Martine L and Stemmer-Rachamimov, Anat O and Rabkin, Samuel D and Weissleder, Ralph and Martuza, Robert L and others, undefined
    Journal: <\/strong>Clinical Cancer Research (2011): 4484–4493<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Referencias<\/mark><\/p>\n\n\n\n

    Ver todas las 61 referencias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

    Volatile fatty acid-sensing system involving coenzyme-A transferase
    Authors: <\/strong>Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.
    Journal: <\/strong>Biotechnol Prog (2006): 334<\/p>\n\n\n\n

    Galactose oxidase action on galactose containing glycolipids–a fluorescence method
    Authors: <\/strong>Fortelius M, Mattjus P.
    Journal: <\/strong>Chem Phys Lipids (2006): 103<\/p>\n\n\n\n

    Effects of chronic ethanol administration on the activities and relative synthetic rates of myelin and synaptosomal plasma membrane-associated sialidase in the rat brain
    Authors: <\/strong>Azuine MA, Patel SJ, Lakshman MR.
    Journal: <\/strong>Neurochem Int (2006): 67<\/p>\n\n\n\n

    Mitochondrial reactive oxygen species in mice lacking superoxide dismutase 2: attenuation via antioxidant treatment
    Authors: <\/strong>Morten KJ, Ackrell BA, Melov S.
    Journal: <\/strong>J Biol Chem (2006): 3354<\/p>\n\n\n\n

    A new, sensitive method for enzyme kinetic studies of scarce glucosides
    Authors: <\/strong>Mazura P, Fohlerova R, Brzobohaty B, Kiran NS, J and a L., undefined
    Journal: <\/strong>J Biochem Biophys Methods (2006): 55<\/p>\n\n\n\n

    Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species
    Authors: <\/strong>Rinaldi M, Moroni P, Paape MJ, Bannerman DD.
    Journal: <\/strong>Vet Immunol Immunopathol. (2006)<\/p>\n\n\n\n

    Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
    Authors: <\/strong>Fruk L, Muller J, Niemeyer CM.
    Journal: <\/strong>Chemistry (2006): 7448<\/p>\n\n\n\n

    K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling
    Authors: <\/strong>Drose S, Br and t U, Hanley PJ.
    Journal: <\/strong>J Biol Chem (2006): 23733<\/p>\n\n\n\n

    A Role for Mitochondrial Dysfunction in Perpetuating Radiation-Induced Genomic Instability
    Authors: <\/strong>Kim GJ, Fiskum GM, Morgan WF.
    Journal: <\/strong>Cancer Res (2006): 10377<\/p>\n\n\n\n

    Sexual Dimorphism in Oxidant Status in Spontaneously Hypertensive Rats
    Authors: <\/strong>Sullivan JC, Sasser JM, Pollock JS.
    Journal: <\/strong>Am J Physiol Regul Integr Comp Physiol. (2006)<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Application notes <\/mark><\/p>\n\n\n\n

    A Library of Well-Defined and Water-Soluble Poly(alkyl phosphonate)s with Adjustable Hydrolysis<\/a>
    Acetylcholinesterase Inhibitory Activity of Pigment Echinochrome A<\/a>
    Ameliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer’s Disease<\/a>
    An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults<\/a>
    Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity<\/a><\/p>\n\n\n\n

    <\/div>\n\n\n\n

    FAQ<\/mark><\/p>\n\n\n\n

    Are coumarins water-soluble?<\/a>
    Are NADH and ROS related?<\/a>
    Can I measure NADPH without lysing my cells?<\/a>
    Can I use Amplite Fluorimetric Glutamic Acid Assay Kit with an absorbance microplate reader?<\/a>
    How are fatty acids activated?<\/a><\/p>\n\n\n\n

    <\/div>\n\n\n\n

    AssayWise <\/mark><\/p>\n\n\n\n

    Peroxidase Detection<\/a>
    Acetylcholinesterase Quantification<\/a>
    Acetylcholinesterase Assays<\/a>
    Glycerol 3-Phosphate Assays<\/a>
    Catalase Detection<\/a><\/p>\n\n\n\n

    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Amplite\u00ae Red es una de las sondas fluorog\u00e9nicas m\u00e1s sensibles y estables para detectar HRP y H2O2. <\/p>\n","protected":false},"featured_media":8107,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8105"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":4,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8105\/revisions"}],"predecessor-version":[{"id":8125,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8105\/revisions\/8125"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8107"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8105"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8105"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}