{"id":8096,"date":"2023-03-09T16:52:08","date_gmt":"2023-03-09T22:52:08","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=8096"},"modified":"2023-03-09T17:11:06","modified_gmt":"2023-03-09T23:11:06","slug":"readiuse-tmb-substrate-solution","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/readiuse-tmb-substrate-solution\/","title":{"rendered":"ReadiUse\u2122 TMB Substrate Solution"},"content":{"rendered":"\n

Los conjugados de peroxidasa de Horseradish (HRP) y HRP facilitan la oxidaci\u00f3n de TMB en presencia de per\u00f3xido de hidr\u00f3geno, convirtiendo a TMB en su producto oxidado azul. <\/p>\n\n\n\n

ReadiUse\u2122 TMB Substrate Solution es una soluci\u00f3n premezclada de sustrato TMB con per\u00f3xido de hidr\u00f3geno. Produce un producto azul al interactuar con HRP o conjugados de HRP sin la adici\u00f3n de per\u00f3xido de hidr\u00f3geno. El producto azul soluble se puede cuantificar a 650 nm. El uso de una soluci\u00f3n de parada aumenta la sensibilidad de 2 a 4 veces y la soluci\u00f3n amarilla resultante se puede leer a 450 nm. <\/p>\n\n\n\n

La soluci\u00f3n de sustrato ReadiUse\u2122 TMB proporciona un sistema de sustrato cuantitativo conveniente y ultrasensible para Ensayos de ELISA con conjugados HRP.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-11012<\/td>ReadiUse\u2122 TMB Substrate Solution<\/td>100 ml<\/td><\/tr>
AAT-11003<\/td>ReadiUse\u2122 TMB Substrate Solution<\/td>1 L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Refrigeraci\u00f3n a 2-8\u00a0\u00b0C. MInimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Absorbancia<\/mark><\/p>\n\n\n\n

Absorbancia (nm)<\/td>650<\/td><\/tr>
Placa recomendada <\/td>Fondo transparente<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/strong>
Volumen del solvente apropiado necesario para reconstituir la masa espec\u00edfica de soluci\u00f3n de sustrato ReadiUse\u2122 TMB (optimizada para ensayos ELISA con conjugados HRP<\/em>) a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>416.077 \u00b5L<\/td>2.08 mL<\/td>4.161 mL<\/td>20.804 mL<\/td>41.608 mL<\/td><\/tr>
5 mM<\/strong><\/td>83.215 \u00b5L<\/td>416.077 \u00b5L<\/td>832.154 \u00b5L<\/td>4.161 mL<\/td>8.322 mL<\/td><\/tr>
10 mM<\/strong><\/td>41.608 \u00b5L<\/td>208.039 \u00b5L<\/td>416.077 \u00b5L<\/td>2.08 mL<\/td>4.161 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

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Figura 1. <\/strong>La respuesta a la dosis de HRP se midi\u00f3 con la soluci\u00f3n de sustrato ReadiUse\u2122 TMB en una placa transparente de 96 pocillos utilizando un lector de microplacas SpectraMax (Molecular Devices).<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

ReadiUse\u2122 Preactivated APC-iFluor\u00ae 700 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-iFluor\u00ae 750 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 647 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 750 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 hydrogen peroxide solution *50 mM calibrated and stabilized solution*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated HRP NHS ester<\/a><\/td><\/tr>
ReadiUse\u2122 NADPH Regenerating Kit<\/a><\/td><\/tr>
ReadiUse\u2122 NADP Regenerating Kit<\/a><\/td><\/tr>
ReadiUse\u2122 TCA Deproteinization Sample Preparation Kit<\/a><\/td><\/tr>
ReadiUse\u2122 microscope mounting solution<\/a><\/td><\/tr>
ReadiUse\u2122 4% formaldehyde fixation solution<\/a><\/td><\/tr>
ReadiUse\u2122 mammalian cell lysis buffer *5X*<\/a><\/td><\/tr>
ReadiUse\u2122 probenecid, sodium salt *Water-soluble*<\/a><\/td><\/tr>
ReadiUse\u2122 probenecid *25 mM stabilized aqueous solution*<\/a><\/td><\/tr>
ReadiUse\u2122 CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy5.5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy7 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Texas Red Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-Cy5.5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-Cy7 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 TCEP removal solution<\/a><\/td><\/tr>
ReadiUse\u2122 WST-8 *50 mM aqueous solution*<\/a><\/td><\/tr>
ReadiUse\u2122 Rapid Luminometric ATP Assay Kit<\/a><\/td><\/tr>
ReadiUse\u2122 PE [R-Phycoerythrin] *Ammonium Sulfate-Free*<\/a><\/td><\/tr>
ReadiUse\u2122 CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 594 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Cell Detaching Buffer<\/a><\/td><\/tr>
ReadiUse\u2122 DRAQ5 Staining Solution *5 mM in Water*<\/a><\/td><\/tr>
ReadiUse\u2122 Staurosporine * 1 mM DMSO stock solution*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 42 referencias:\u00a0Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Peroxidase-like activity of apoferritin paired gold clusters for glucose detection
Authors: <\/strong>Jiang X, Sun C, Guo Y, Nie G, Xu L.
Journal: <\/strong>Biosens Bioelectron (2014): 165<\/p>\n\n\n\n

Novel tungsten carbide nanorods: an intrinsic peroxidase mimetic with high activity and stability in aqueous and organic solvents
Authors: <\/strong>Li N, Yan Y, Xia BY, Wang JY, Wang X.
Journal: <\/strong>Biosens Bioelectron (2014): 521<\/p>\n\n\n\n

Glucose-sensitive colorimetric sensor based on peroxidase mimics activity of porphyrin-Fe3O4 nanocomposites
Authors: <\/strong>Liu Q, Li H, Zhao Q, Zhu R, Yang Y, Jia Q, Bian B, Zhuo L.
Journal: <\/strong>Mater Sci Eng C Mater Biol Appl (2014): 142<\/p>\n\n\n\n

Peroxidase-Like Catalytic Activity of Ag3PO4 Nanocrystals Prepared by a Colloidal Route
Authors: <\/strong>Liu Y, Zhu G, Yang J, Yuan A, Shen X.
Journal: <\/strong>PLoS One (2014): e109158<\/p>\n\n\n\n

Colorimetric estimation of human glucose level using gamma-Fe(2)O(3) nanoparticles: an easily recoverable effective mimic peroxidase
Authors: <\/strong>Mitra K, Ghosh AB, Sarkar A, Saha N, Dutta AK.
Journal: <\/strong>Biochem Biophys Res Commun (2014): 30<\/p>\n\n\n\n

Visual detection of blood glucose based on peroxidase-like activity of WS2 nanosheets
Authors: <\/strong>Lin T, Zhong L, Song Z, Guo L, Wu H, Guo Q, Chen Y, Fu F, Chen G.
Journal: <\/strong>Biosens Bioelectron (2014): 302<\/p>\n\n\n\n

5,10,15,20-tetrakis(4-carboxyl phenyl)porphyrin-CdS nanocomposites with intrinsic peroxidase-like activity for glucose colorimetric detection
Authors: <\/strong>Liu Q, Jia Q, Zhu R, Shao Q, Wang D, Cui P, Ge J.
Journal: <\/strong>Mater Sci Eng C Mater Biol Appl (2014): 177<\/p>\n\n\n\n

Biogenic magnetic nanoparticles from Burkholderia sp. YN01 exhibiting intrinsic peroxidase-like activity and their applications
Authors: <\/strong>Pan Y, Li N, Mu J, Zhou R, Xu Y, Cui D, Wang Y, Zhao M.
Journal: <\/strong>Appl Microbiol Biotechnol. (2014)<\/p>\n\n\n\n

Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates
Authors: <\/strong>Aumiller WM, Jr., Davis BW, Hatzakis E, Keating CD.
Journal: <\/strong>J Phys Chem B (2014): 10624<\/p>\n\n\n\n

Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance
Authors: <\/strong>Chen X, Tian X, Su B, Huang Z, Oyama M.
Journal: <\/strong>Dalton Trans (2014): 7449<\/p>\n\n\n\n

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Application notes <\/mark><\/p>\n\n\n\n

A Meta-Analysis of Common Calcium Indicator<\/a>s<\/a>
A <\/a>New Protein Crosslinking Method for Labeling and Modifying Antibod<\/a>ies<\/a>
A <\/a>Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Ce<\/a>lls<\/a>
A<\/a>bbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

What is a Direct ELISA?<\/a>
What is an Indirect ELISA?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a>
Are there any alternatives to Cy5?<\/a>
Are there any alternatives to indocyanine green (ICG)?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise <\/mark><\/p>\n\n\n\n

Peroxidase Detection<\/a>
ReadiView\u2122 Biotin: All-In-One Biotinylation & Quantification of Biotin Labeling<\/a>
HRP Antibody Labeling Using Buccutite\u2122 Crosslinking Technology<\/a>
iFluor\u00ae 700 Dyes<\/a>
Hydroxyl Radical Detection<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Soluci\u00f3n de sustrato ReadiUse\u2122 TMB, Optimizada para ensayos ELISA con conjugados HRP. <\/p>\n","protected":false},"featured_media":8097,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8096"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8096\/revisions"}],"predecessor-version":[{"id":8099,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/8096\/revisions\/8099"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/8097"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=8096"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=8096"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}