{"id":7967,"date":"2023-03-08T17:50:22","date_gmt":"2023-03-08T23:50:22","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=7967"},"modified":"2023-03-08T18:14:19","modified_gmt":"2023-03-09T00:14:19","slug":"readiuse-preactivated-hrp-nhs-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/readiuse-preactivated-hrp-nhs-ester\/","title":{"rendered":"ReadiUse\u2122 Preactivated HRP NHS ester"},"content":{"rendered":"\n

ReadiUse\u2122 Preactivated HRP NHS \u00e9ster es el mono NHS \u00e9ster de HRP. Se puede usar para marcar f\u00e1cilmente prote\u00ednas (como anticuerpos) u otras mol\u00e9culas biol\u00f3gicas que tienen un grupo amino. <\/p>\n\n\n\n

El \u00e9ster HRP NHS preactivado ReadiUse\u2122 es robusto y f\u00e1cil de usar. Se puede utilizar para marcar anticuerpos con una simple mezcla, es decir, la soluci\u00f3n b\u00e1sica de un anticuerpo (pH 8,5-9,0) se puede mezclar directamente con el \u00e9ster HRP NHS y la mezcla de reacci\u00f3n se agita durante 1-2 horas. En la mayor\u00eda de los casos, la soluci\u00f3n resultante puede usarse directamente para ensayos ELISA sin m\u00e1s purificaci\u00f3n. <\/p>\n\n\n\n

ReadiUse\u2122 Preactivated HRP NHS \u00e9ster proporciona el m\u00e9todo m\u00e1s s\u00f3lido para realizar la conjugaci\u00f3n de anticuerpos con HRP. Cualquier cantidad adecuada de anticuerpo (>1 mg\/mL) se puede conjugar f\u00e1cilmente con el \u00e9ster NHS de HRP preactivado sin necesidad de purificaci\u00f3n.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-11025<\/td>ReadiUse\u2122 Preactivated HRP NHS ester<\/td>300 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n a < -15 \u00b0C. Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1.<\/strong> Mecanismo para la conjugaci\u00f3n de HRP a la prote\u00edna objetivo usando \u00e9ster de NHS activado.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 12 bibliografias:\u00a0Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Florfenicol binding to molecularly imprinted polymer nanoparticles in model and real samples<\/a>
Authors: <\/strong>Caro, Nelson and Bruna, Tamara and Guerreiro, Antonio and Alvarez-Tejos, Paola and Garret{\\’o}n, Virginia and Piletsky, Sergey and Gonz{\\’a}lez-Casanova, Jorge and Rojas-G{\\’o}mez, Diana and Ehrenfeld, Nicole
Journal: <\/strong>Nanomaterials (2020): 306<\/p>\n\n\n\n

New method for immobilising diverse proteins onto cubic micro-protein polyhedrin crystals<\/a>
Authors: <\/strong>Yuasa, Haruna and Kotani, Eiji and Mori, Hajime and Takaki, Keiko
Journal: <\/strong>Protein expression and purification (2019): 105531<\/p>\n\n\n\n

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase<\/a>
Authors: <\/strong>Milton, Amber
Journal: <\/strong>(2017)<\/p>\n\n\n\n

Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA<\/a>
Authors: <\/strong>Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: <\/strong>Analytical Chemistry (2017)<\/p>\n\n\n\n

Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury<\/a>
Authors: <\/strong>Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: <\/strong>Radiology (2016): 152222<\/p>\n\n\n\n

Myeloperoxidase–Hepatocyte–Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis<\/a>
Authors: <\/strong>Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W
Journal: <\/strong>Antioxidants &amp; redox signaling (2015): 1255–1269<\/p>\n\n\n\n

Myeloperoxidase Nuclear Imaging for Epileptogenesis<\/a>
Authors: <\/strong>Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others, undefined
Journal: <\/strong>Radiology (2015): 822–830<\/p>\n\n\n\n

Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs<\/a>
Authors: <\/strong>Huang, Jiansheng and Smith, Forrest and Panizzi, Peter
Journal: <\/strong>Archives of biochemistry and biophysics (2014): 74–85<\/p>\n\n\n\n

Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings<\/a>
Authors: <\/strong>Scherag, Frank D and Br, undefined and stetter, Thomas and R&uuml;he, J&uuml;rgen
Journal: <\/strong>Colloids and Surfaces B: Biointerfaces (2014): 576–582<\/p>\n\n\n\n

Measuring myeloperoxidase activity in biological samples<\/a>
Authors: <\/strong>Pulli, Benjamin and Ali, Muhammad and Forghani, Reza and Schob, Stefan and Hsieh, Kevin LC and Wojtkiewicz, Gregory and Linnoila, Jenny J and Chen, John W
Journal: <\/strong>PLoS One (2013): e67976<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 61 referencias:\u00a0Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Volatile fatty acid-sensing system involving coenzyme-A transferase
Authors: <\/strong>Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.
Journal: <\/strong>Biotechnol Prog (2006): 334<\/p>\n\n\n\n

Galactose oxidase action on galactose containing glycolipids–a fluorescence method
Authors: <\/strong>Fortelius M, Mattjus P.
Journal: <\/strong>Chem Phys Lipids (2006): 103<\/p>\n\n\n\n

Effects of chronic ethanol administration on the activities and relative synthetic rates of myelin and synaptosomal plasma membrane-associated sialidase in the rat brain
Authors: <\/strong>Azuine MA, Patel SJ, Lakshman MR.
Journal: <\/strong>Neurochem Int (2006): 67<\/p>\n\n\n\n

Mitochondrial reactive oxygen species in mice lacking superoxide dismutase 2: attenuation via antioxidant treatment
Authors: <\/strong>Morten KJ, Ackrell BA, Melov S.
Journal: <\/strong>J Biol Chem (2006): 3354<\/p>\n\n\n\n

A new, sensitive method for enzyme kinetic studies of scarce glucosides
Authors: <\/strong>Mazura P, Fohlerova R, Brzobohaty B, Kiran NS, J and a L., undefined
Journal: <\/strong>J Biochem Biophys Methods (2006): 55<\/p>\n\n\n\n

Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species
Authors: <\/strong>Rinaldi M, Moroni P, Paape MJ, Bannerman DD.
Journal: <\/strong>Vet Immunol Immunopathol. (2006)<\/p>\n\n\n\n

Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
Authors: <\/strong>Fruk L, Muller J, Niemeyer CM.
Journal: <\/strong>Chemistry (2006): 7448<\/p>\n\n\n\n

K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling
Authors: <\/strong>Drose S, Br and t U, Hanley PJ.
Journal: <\/strong>J Biol Chem (2006): 23733<\/p>\n\n\n\n

A Role for Mitochondrial Dysfunction in Perpetuating Radiation-Induced Genomic Instability
Authors: <\/strong>Kim GJ, Fiskum GM, Morgan WF.
Journal: <\/strong>Cancer Res (2006): 10377<\/p>\n\n\n\n

Sexual Dimorphism in Oxidant Status in Spontaneously Hypertensive Rats
Authors: <\/strong>Sullivan JC, Sasser JM, Pollock JS.
Journal: <\/strong>Am J Physiol Regul Integr Comp Physiol. (2006)<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application notes <\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

Can I use azide in ELISA buffers?<\/a>
What is HRP conjugated secondary antibody?<\/a>
What label should I choose for a secondary antibody?<\/a>
Why is an HRP conjugated secondary antibody used in western blotting?<\/a>
Are any of the cyanine dyes infrared?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise <\/mark><\/p>\n\n\n\n

A practical guide for use of PE and APC in flow cytometry<\/a>
Calbryte\u2122 series now available<\/a>
Buccutite\u2122 Fluorescent Protein and Tandem Dye Antibody Labeling Kits<\/a>
Fundamentals of Flow Cytometry<\/a>
ReadiUse\u2122 Lyophilized Phycobiliproteins<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

ReadiUse\u2122 Preactivated HRP NHS \u00e9ster proporciona el m\u00e9todo m\u00e1s s\u00f3lido para realizar la conjugaci\u00f3n de anticuerpos con HRP. <\/p>\n","protected":false},"featured_media":7968,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/7967"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/7967\/revisions"}],"predecessor-version":[{"id":7970,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/7967\/revisions\/7970"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/7968"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=7967"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=7967"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}