{"id":7957,"date":"2023-03-08T16:33:46","date_gmt":"2023-03-08T22:33:46","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=7957"},"modified":"2023-03-08T16:57:34","modified_gmt":"2023-03-08T22:57:34","slug":"horseradish-peroxidase-hrp-optimizada-para-conjugacion","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/horseradish-peroxidase-hrp-optimizada-para-conjugacion\/","title":{"rendered":"Horseradish Peroxidase (HRP) *Optimizada para conjugacion*"},"content":{"rendered":"\n

La peroxidasa de r\u00e1bano picante (HRP) es una glicoprote\u00edna con un peso molecular de ~44 KD. Se a\u00edsla de ra\u00edces de r\u00e1bano picante (Amoracia rusticana) y pertenece al grupo de peroxidasas de las ferroprotoporfirinas. HRP es un polip\u00e9ptido de cadena sencilla que contiene cuatro puentes disulfuro. Es una glicoprote\u00edna que contiene un 18% de carbohidratos. La composici\u00f3n de carbohidratos consiste en galactosa, arabinosa, xilosa, fucosa, manosa, manosamina y galactosamina. El HRP se combina f\u00e1cilmente con el per\u00f3xido de hidr\u00f3geno (H2O2) y el complejo [HRP-H2O2] resultante puede oxidar una amplia variedad de donantes cromog\u00e9nicos de hidr\u00f3geno. <\/p>\n\n\n\n

AAT Bioquest ofrece la mayor colecci\u00f3n de sustratos y conjugados de HRP. HRP se usa ampliamente como marcador para inmunoglobulinas en muchas aplicaciones diferentes de inmunoqu\u00edmica, que incluyen ELISA, inmunotransferencia e inmunohistoqu\u00edmica. La HRP se puede conjugar con anticuerpos mediante varios m\u00e9todos diferentes, incluido el glutaraldeh\u00eddo, la oxidaci\u00f3n con peryodato, a trav\u00e9s de enlaces disulfuro y tambi\u00e9n a trav\u00e9s de entrecruzadores dirigidos por amino y tiol. Nuestra t\u00e9cnica de conjugaci\u00f3n Buccutite\u2122 HRP es, con diferencia, el m\u00e9todo m\u00e1s eficaz. HRP es el marcador m\u00e1s deseado para los anticuerpos, ya que es el m\u00e1s peque\u00f1o y estable de los tres marcadores enzim\u00e1ticos m\u00e1s populares (HRP, fosfatasa alcalina y B-galactosidasa), y su glicosilaci\u00f3n conduce a una menor uni\u00f3n no espec\u00edfica. <\/p>\n\n\n\n

Esta peroxidasa de r\u00e1bano picante (HRP) purificada se suministra liofilizada como un polvo sin sal para reconstituci\u00f3n y uso en detecciones o conjugaciones de HRP.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-11021<\/td>Horseradish Peroxidase *Optimized for conjugation*<\/td>10 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelacion a \u00a0<-15 \u00b0C. Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Propiedades F\u00edsicas<\/mark><\/p>\n\n\n\n

Disolvente<\/td>Agua<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1.<\/strong> La peroxidasa de r\u00e1bano picante (HRP) es una glicoprote\u00edna con un peso molecular de ~44 KD. Se a\u00edsla de ra\u00edces de r\u00e1bano picante (Amoracia rusticana) y pertenece al grupo de peroxidasas de las ferroprotoporfirinas. HRP es un polip\u00e9ptido de cadena sencilla que contiene cuatro puentes disulfuro. Es una glicoprote\u00edna que contiene un 18% de carbohidratos. La composici\u00f3n de carbohidratos consiste en galactosa, arabinosa, xilosa, fucosa, manosa, manosamina y galactosamina. El HRP se combina f\u00e1cilmente con el per\u00f3xido de hidr\u00f3geno (H2O2) y el complejo [HRP-H2O2] resultante puede oxidar una amplia variedad de donantes cromog\u00e9nicos de hidr\u00f3geno. HRP se usa ampliamente como marcador para inmunoglobulinas en muchas aplicaciones diferentes de inmunoqu\u00edmica, que incluyen ELISA, inmunotransferencia e inmunohistoqu\u00edmica. AAT Bioquest ofrece la mayor colecci\u00f3n de sustratos y conjugados de HRP.<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Verw todas las 50 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Nanocapsulation of horseradish peroxidase (HRP) enhances enzymatic performance in removing phenolic compounds.
Authors: <\/strong>Liu, Shan and Huang, Biyan and Zheng, Guiqin and Zhang, Peng and Li, Juying and Yang, Bo and Chen, Yantao and Liang, Li
Journal: <\/strong>International journal of biological macromolecules (2020): 814-822<\/p>\n\n\n\n

Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins.
Authors: <\/strong>Yang, Hualin and Zhang, Qi and Liu, Xiaolei and Yang, Yuying and Yang, Yong and Liu, Mingyuan and Li, Peiwu and Zhou, Yu
Journal: <\/strong>Food chemistry (2020): 126356<\/p>\n\n\n\n

An efficient methodology for the purification of date palm peroxidase: Stability comparison with horseradish peroxidase (HRP).
Authors: <\/strong>Saud Al-Bagmi, Moneera and Shahnawaz Khan, Mohd and Alhasan Ismael, Mohamad and Al-Senaidy, Abdulrahman M and Ben Bacha, Abir and Mabood Husain, Fohad and Alamery, Salman Freeh
Journal: <\/strong>Saudi journal of biological sciences (2019): 301-307<\/p>\n\n\n\n

Sensitive sulfide ion detection by optofluidic catalytic laser using horseradish peroxidase (HRP) enzyme.
Authors: <\/strong>Gong, Chaoyang and Gong, Yuan and Khaing Oo, Maung Kyaw and Wu, Yu and Rao, Yunjiang and Tan, Xiaotian and Fan, Xudong
Journal: <\/strong>Biosensors & bioelectronics (2017): 351-357<\/p>\n\n\n\n

Ultrasensitive electrochemical immunosensor based on horseradish peroxidase (HRP)-loaded silica-poly(acrylic acid) brushes for protein biomarker detection.
Authors: <\/strong>Zhao, Yan and Zheng, Yiqun and Kong, Rongmei and Xia, Lian and Qu, Fengli
Journal: <\/strong>Biosensors & bioelectronics (2016): 383-8<\/p>\n\n\n\n

Bioconjugation of Antibodies to Horseradish Peroxidase (HRP).
Authors: <\/strong>Hnasko, Robert M
Journal: <\/strong>Methods in molecular biology (Clifton, N.J.) (2015): 43-50<\/p>\n\n\n\n

Pb(2+)-introduced activation of horseradish peroxidase (HRP)-mimicking DNAzyme.
Authors: <\/strong>Zhu, Xi and Gao, Xiaoyao and Liu, Qida and Lin, Zhenyu and Qiu, Bin and Chen, Guonan
Journal: <\/strong>Chemical communications (Cambridge, England) (2011): 7437-9<\/p>\n\n\n\n

Toxicity of textile dyes and their degradation by the enzyme horseradish peroxidase (HRP).
Authors: <\/strong>Ulson de Souza, Selene Maria Arruda Guelli and Forgiarini, Eliane and Ulson de Souza, Ant\u00f4nio Augusto
Journal: <\/strong>Journal of hazardous materials (2007): 1073-8<\/p>\n\n\n\n

Studies on the oxidation reaction of tyrosine (Tyr) with H2O2 catalyzed by horseradish peroxidase (HRP) in alcohol-water medium by spectrofluorimetry and differential spectrophotometry.
Authors: <\/strong>Tang, Bo and Wang, Yan and Liang, Huiling and Chen, Zhenzhen and He, Xiwen and Shen, Hanxi
Journal: <\/strong>Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2006): 609-13<\/p>\n\n\n\n

FIA-near-infrared spectrofluorimetric trace determination of hydrogen peroxide using tricarchlorobocyanine dye (Cy.7.Cl) and horseradish peroxidase (HRP).
Authors: <\/strong>Tang, Bo and Zhang, Li and Xu, Ke-hua
Journal: <\/strong>Talanta (2006): 876-82<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application notes <\/mark><\/p>\n\n\n\n

A Meta-Analysis of Common Calcium Indicators<\/a>
A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a>
Are there any alternatives to Cy5?<\/a>
Are<\/a> <\/a>there any alternatives to indocyanine green (ICG)?<\/a>
Can DAPI <\/a>b<\/a>ind to RNA?<\/a>
Can DAP<\/a>I<\/a> stain dead cells?<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise <\/mark><\/p>\n\n\n\n

HRP Antibody Labeling Using Buccutite\u2122 Crosslinking Technology<\/a>
iFluor\u00ae 700 Dyes<\/a>
Hydroxyl Radical Detection<\/a>
Peroxidase Detection<\/a>
Buccutite\u2122 Conjugation Kits: Quick and Easy Antibody Labeling<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

HRP se usa ampliamente como marcador para inmunoglobulinas en muchas aplicaciones diferentes de inmunoqu\u00edmica, que incluyen ELISA, inmunotransferencia e inmunohistoqu\u00edmica. <\/p>\n","protected":false},"featured_media":7959,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/7957"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/7957\/revisions"}],"predecessor-version":[{"id":7961,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/7957\/revisions\/7961"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/7959"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=7957"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=7957"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}