{"id":7635,"date":"2023-03-01T21:54:47","date_gmt":"2023-03-02T03:54:47","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=7635"},"modified":"2023-03-01T22:15:28","modified_gmt":"2023-03-02T04:15:28","slug":"calbryte-590-potassium-salt","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/calbryte-590-potassium-salt\/","title":{"rendered":"Calbryte\u2122 590, potassium salt"},"content":{"rendered":"\n

El ensayo de flujo de calcio intracelular es un m\u00e9todo ampliamente utilizado en el monitoreo de las v\u00edas de transducci\u00f3n de se\u00f1ales y el cribado de alto rendimiento de los receptores acoplados a prote\u00ednas G (GPCR) y los objetivos de los canales de calcio. Seguidos por la introducci\u00f3n de Rhod-2 en 1989, Rhod-4 y Cal-590 se desarrollaron m\u00e1s tarde con una relaci\u00f3n se\u00f1al \/ fondo mejorada, y se convirtieron en los indicadores fluorescentes rojos de Ca2 + ampliamente utilizados para microscop\u00eda confocal, citometr\u00eda de flujo y aplicaciones de detecci\u00f3n de alto rendimiento.<\/p>\n\n\n\n

Calbryte\u2122 590 es una nueva generaci\u00f3n de indicadores fluorescentes rojos para la medici\u00f3n del calcio intracelular. Su relaci\u00f3n se\u00f1al\/fondo muy mejorada y sus propiedades de retenci\u00f3n intracelular hacen de Calbryte\u2122 590 el indicador fluorescente rojo m\u00e1s robusto para evaluar GPCR y objetivos de canales de calcio, as\u00ed como para detectar sus agonistas y antagonistas en c\u00e9lulas vivas.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-20706<\/td>Calbryte\u2122 590, potassium salt<\/td>5 x 50 ug<\/td><\/tr>
AAT-20707<\/td>Calbryte\u2122 590, potassium salt<\/td>1 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar a  (< -15 \u00b0C), Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en\u00a0Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>581<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>593<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de agua necesario para reconstituir la masa espec\u00edfica de Calbryte\u2122 590, sal de potasio a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para obtener los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>93.025 \u00b5L<\/td>465.125 \u00b5L<\/td>930.25 \u00b5L<\/td>4.651 mL<\/td>9.302 mL<\/td><\/tr>
5 mM<\/strong><\/td>18.605 \u00b5L<\/td>93.025 \u00b5L<\/td>186.05 \u00b5L<\/td>930.25 \u00b5L<\/td>1.86 mL<\/td><\/tr>
10 mM<\/strong><\/td>9.302 \u00b5L<\/td>46.512 \u00b5L<\/td>93.025 \u00b5L<\/td>465.125 \u00b5L<\/td>930.25 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1.<\/strong> Espectros de emisi\u00f3n de fluorescencia de Calbryte\u2122 590 en soluci\u00f3n que contiene 0 a 39 \u03bcM libre Ca2+.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Relacionados<\/mark><\/p>\n\n\n\n

Calbryte\u2122 520 AM<\/a><\/td><\/tr>
Calbryte\u2122 590 AM<\/a><\/td><\/tr>
Calbryte\u2122 630 AM<\/a><\/td><\/tr>
Calbryte\u2122-520L AM<\/a><\/td><\/tr>
Calbryte\u2122-520XL azide<\/a><\/td><\/tr>
Calbryte\u2122-520XL AM<\/a><\/td><\/tr>
Calbryte\u2122-520XL-Dextran<\/a><\/td><\/tr>
Chemical Phosphorylation Reagent I (CPR I)<\/a><\/td><\/tr>
Cell Meter\u2122 Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*<\/a><\/td><\/tr>
Cal Green\u2122 1, hexapotassium salt<\/a><\/td><\/tr>
Cal Green\u2122 1, AM [Equivalent to Calcium Green-1, AM]<\/a><\/td><\/tr>
Cal-590\u2122-Dextran Conjugate *MW 3,000*<\/a><\/td><\/tr>
Cal-590\u2122-Dextran Conjugate *MW 10,000*<\/a><\/td><\/tr>
Cal-590\u2122 AM<\/a><\/td><\/tr>
Cal-590\u2122, sodium salt<\/a><\/td><\/tr>
Cal-630\u2122 AM<\/a><\/td><\/tr>
Cal-630\u2122, sodium salt<\/a><\/td><\/tr>
Cal-630\u2122, potassium salt<\/a><\/td><\/tr>
Cal-630\u2122-Dextran Conjugate *MW 3,000*<\/a><\/td><\/tr>
Cal-630\u2122-Dextran Conjugate *MW 10,000*<\/a><\/td><\/tr>
Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*<\/a><\/td><\/tr>
Fluo-4, Pentapotassium Salt<\/a><\/td><\/tr>
Cal Red\u2122 R525\/650 potassium salt<\/a><\/td><\/tr>
Cal Red\u2122 R525\/650 AM<\/a><\/td><\/tr>
Cal-520\u00ae-Dextran Conjugate *MW 3,000*<\/a><\/td><\/tr>
Cal-520\u00ae-Dextran Conjugate *MW 10,000*<\/a><\/td><\/tr>
Cal-520\u00ae-Biotin Conjugate<\/a><\/td><\/tr>
Cal-520\u00ae-Biocytin Conjugate<\/a><\/td><\/tr>
Cal-520\u00ae NHS Ester<\/a><\/td><\/tr>
Cal-520\u00ae maleimide<\/a><\/td><\/tr>
Fluo-3, AM *CAS 121714-22-5*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/mark><\/mark><\/p>\n\n\n\n

Calreticulin regulates TGF-&beta;1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling<\/a>
Authors: <\/strong>Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
Journal: <\/strong>The International Journal of Biochemistry &amp; Cell Biology (2017)<\/p>\n\n\n\n

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells<\/a>
Authors: <\/strong>Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
Journal: <\/strong>Neural Regeneration Research (2017): 945<\/p>\n\n\n\n

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels<\/a>
Authors: <\/strong>Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
Journal: <\/strong>Scientific Reports (2017): 41781<\/p>\n\n\n\n

Dexmedetomidine reduces hypoxia\/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons<\/a>
Authors: <\/strong>Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
Journal: <\/strong>Int J Clin Exp Med (2017): 6861–6868<\/p>\n\n\n\n

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection<\/a>
Authors: <\/strong>Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
Journal: <\/strong>Journal of cellular and molecular medicine (2015): 581–594<\/p>\n\n\n\n

The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia\/reperfusion injury<\/a>
Authors: <\/strong>Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia
Journal: <\/strong>Biochemical and biophysical research communications (2015): 537–542<\/p>\n\n\n\n

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats<\/a>
Authors: <\/strong>Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min
Journal: <\/strong>Journal of Neural Transmission (2014): 593–600<\/p>\n\n\n\n

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem\/progenitor cells<\/a>
Authors: <\/strong>Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined
Journal: <\/strong>Neural regeneration research (2014): 2002<\/p>\n\n\n\n

Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.<\/a>
Authors: <\/strong>Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui
Journal: <\/strong>International journal of ophthalmology (2014): 441–447<\/p>\n\n\n\n

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis<\/a>
Authors: <\/strong>Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A
Journal: <\/strong>British Journal of Dermatology (2013): 771–778<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las\u00a053 referencias:\u00a0Citation Explorer<\/a><\/em><\/p>\n\n\n\n

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: <\/strong>Bailey S, Macardle PJ.
Journal: <\/strong>J Immunol Methods (2006): 220<\/p>\n\n\n\n

Functional fluo-3\/AM assay on P-glycoprotein transport activity in L1210\/VCR cells by confocal microscopy
Authors: <\/strong>Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: <\/strong>Gen Physiol Biophys (2004): 357<\/p>\n\n\n\n

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry
Authors: <\/strong>Patel H, Porter RH, Palmer AM, Croucher MJ.
Journal: <\/strong>Br J Pharmacol (2003): 671<\/p>\n\n\n\n

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics
Authors: <\/strong>Loughrey CM, MacEachern KE, Cooper J, Smith GL.
Journal: <\/strong>Cell Calcium (2003): 1<\/p>\n\n\n\n

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification
Authors: <\/strong>Zhang Z, Kitching P.
Journal: <\/strong>J Virol Methods (2000): 187<\/p>\n\n\n\n

Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang
Authors: <\/strong>Rockwell PL, Storey BT.
Journal: <\/strong>Mol Reprod Dev (2000): 335<\/p>\n\n\n\n

MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells
Authors: <\/strong>Cantz T, Nies AT, Brom M, Hofmann AF, Keppler D.
Journal: <\/strong>Am J Physiol Gastrointest Liver Physiol (2000): G522<\/p>\n\n\n\n

Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: <\/strong>Walczysko P, Wagner E, Albrechtova JT.
Journal: <\/strong>Cell Calcium (2000): 23<\/p>\n\n\n\n

[Ca2+]i following extrasystoles in guinea-pig trabeculae microinjected with fluo-3 – a comparison with frog skeletal muscle fibres
Authors: <\/strong>Wohlfart B., undefined
Journal: <\/strong>Acta Physiol Scand (2000): 1<\/p>\n\n\n\n

Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+) concentrations
Authors: <\/strong>Rockwell PL, Storey BT.
Journal: <\/strong>Mol Reprod Dev (1999): 418<\/p>\n\n\n\n

<\/div>\n\n\n\n

Aplication Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells<\/a>
A Meta-Analysis of Common Calcium Indicators<\/a>
A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a>
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets<\/a>
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Calbryte\u2122 590 es una nueva generaci\u00f3n de indicadores fluorescentes rojos para la medici\u00f3n del calcio intracelular.<\/p>\n","protected":false},"featured_media":7636,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/7635"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/7635\/revisions"}],"predecessor-version":[{"id":7638,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/7635\/revisions\/7638"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/7636"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=7635"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=7635"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}