{"id":4529,"date":"2023-02-05T20:56:50","date_gmt":"2023-02-06T02:56:50","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=4529"},"modified":"2023-02-05T21:35:42","modified_gmt":"2023-02-06T03:35:42","slug":"screen-quest-colorimetric-glucose-uptake-assay-kit","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/screen-quest-colorimetric-glucose-uptake-assay-kit\/","title":{"rendered":"Screen Quest\u2122 Colorimetric Glucose Uptake Assay Kit"},"content":{"rendered":"\n

Los sistemas de transporte de glucosa son responsables de transportar la glucosa a trav\u00e9s de las membranas celulares. La medici\u00f3n de la absorci\u00f3n de 2-desoxiglucosa (2-DG), un an\u00e1logo de la glucosa, en tejidos y c\u00e9lulas es ampliamente aceptada como un m\u00e9todo confiable para estimar la cantidad de glucosa absorbida e investigar la regulaci\u00f3n del metabolismo de la glucosa y el mecanismo de resistencia a la insulina. La captaci\u00f3n de 2-DG se determina com\u00fanmente mediante el uso de 2-DG no metabolizado marcado con tritio o C14. Sin embargo, el uso rutinario de una sonda radiomarcada es costoso y requiere un tedioso procedimiento de manipulaci\u00f3n especial. <\/p>\n\n\n\n

El kit de ensayo colorim\u00e9trico de captaci\u00f3n de glucosa Screen Quest\u2122 de AAT Bioquest proporciona un ensayo sensible y no radiactivo en tejidos o c\u00e9lulas cultivadas. En este ensayo, los transportadores de glucosa captan el 2-DG y lo metabolizan a 2-DG-6-fosfato (2-DG6P). El 2-DG6P no metabolizable se acumula en las c\u00e9lulas y es proporcional a la absorci\u00f3n de glucosa por las c\u00e9lulas. El 2-DG6P acumulado se oxida enzim\u00e1ticamente y genera NADPH, que es monitoreado espec\u00edficamente por un sensor de NADPH cromog\u00e9nico. La se\u00f1al se puede leer con un lector de microplacas de absorci\u00f3n leyendo la proporci\u00f3n de OD a una longitud de onda de 570 nm a 610 nm.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-36503<\/td>Screen Quest\u2122 Colorimetric Glucose Uptake Assay Kit<\/td>100 pruebas<\/td><\/tr>
AAT-36504<\/td>Screen Quest\u2122 Colorimetric Glucose Uptake Assay Kit<\/td>500 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Absorbancia<\/mark> <\/p>\n\n\n\n

Absorbancia<\/td>570\/610 nm<\/td><\/tr>
Placa recomendada<\/td>Fondo claro <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: 2-Deoxyglucose (2-DG, 10mM)<\/td>1 botella (5 mL)<\/td><\/tr>
Componente B: <\/strong>Glucose Uptake Buffer<\/td>1 botella (50 mL)<\/td><\/tr>
Componente C: Acidic Lysis Buffer<\/td>1 botella (12.5 mL)<\/td><\/tr>
Componente D: Neutralization Buffer<\/td>1 botella (12.5 mL)<\/td><\/tr>
Componente E: Enzyme Probe<\/td>5 botellas (polvo liofilizado)<\/td><\/tr>
Componente F: Assay Buffer<\/td>1 botella (25 mL)<\/td><\/tr>
Componente G: NADP<\/td>1 viales<\/td><\/tr>
Componente H: 5x KRPH Buffer<\/td>1 botella (100 mL)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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PREPARACION DE SOLUCION DE STOCK<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em> <\/p>\n\n\n\n

Soluci\u00f3n madre de buffer KRPH (1X): <\/em><\/p>\n\n\n\n

Agregue 20 ml de buffer KRPH (5X) (componente H) a 80 ml de agua desionizada y mezcle bien. Nota:<\/strong> un volumen de 50 ml de buffer KRPH 1\u00d7 es suficiente para aproximadamente una placa de 96 pocillos. Prepare el volumen necesario proporcionalmente. Guarde el 1\u00d7KRPH no utilizado a 4o<\/sup>C o -20o<\/sup>C.<\/p>\n\n\n\n

<\/p>\n\n\n\n

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PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

Soluci\u00f3n de trabajo NADP: <\/em>Agregue 100 \u00b5L de H2O en el vial de NADP (Componente G) y mezcle bien. <\/p>\n\n\n\n

Soluci\u00f3n de trabajo de sonda enzim\u00e1tica: <\/em>Agregue 5 ml de buffer de ensayo (componente F) a la botella de sonda enzim\u00e1tica (componente E) y m\u00e9zclelos bien. <\/p>\n\n\n\n

Soluci\u00f3n de trabajo del ensayo de captaci\u00f3n de 2 DG: <\/em>Agregue 100 \u00b5L de soluci\u00f3n de trabajo de NADP a la soluci\u00f3n de trabajo de Enzyme Probe y m\u00e9zclelos bien. Nota:<\/strong> estas cantidades son buenas para una placa de 96 pocillos.<\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/p>\n<\/div><\/div>\n\n\n\n

<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1. <\/strong>Medici\u00f3n de la captaci\u00f3n de 2DG en adipocitos 3T3-L1 diferenciados y fibroblastos 3T3-L1. Los ensayos se realizaron con el kit de ensayo de captaci\u00f3n de glucosa colorim\u00e9trica Screen Quest\u2122 en una placa de lisina Poly-D de cultivo celular de pared negra\/fondo transparente utilizando un lector de microplacas SpectraMax (Molecular Devices). (A: control negativo, sin insulina, sin tratamiento con 2DG. B: captaci\u00f3n de 2DG en ausencia de insulina. C: captaci\u00f3n de 2DG en presencia de insulina 1 mM. D: captaci\u00f3n de 2DG en presencia de insulina 1 mM y floretina 200 mM. E: captaci\u00f3n de 2DG en presencia de insulina 1 mM y D-glucosa 5 mM.)<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Screen Quest\u2122 Membrane Potential Assay Kit *Orange Fluorescence*<\/a><\/td><\/tr>
Screen Quest\u2122 Membrane Potential Assay Kit *Red Fluorescence*<\/a><\/td><\/tr>
Screen Quest\u2122 10X cell staining buffer with Phenol Red Plus\u2122<\/a><\/td><\/tr>
Screen Quest\u2122 10X calcium assay buffer with Phenol Red Plus\u2122<\/a><\/td><\/tr>
Screen Quest\u2122 Luminometric Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluo-8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines*<\/a><\/td><\/tr>
Screen Quest\u2122 Fluo-8 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fura-2 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Rhod-4 No Wash Calcium Assay Kit *Medium Removal*<\/a><\/td><\/tr>
Screen Quest\u2122 Rhod-4 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric MDR Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Colorimetric Chloride Channel Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Colorimetric ELISA cAMP Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric ELISA cAMP Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 TR-FRET No Wash cAMP Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Live Cell cAMP Assay Service Pack<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric Fatty Acid Uptake Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluo-4 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 No Wash Potassium Channel Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Calbryte-520 Probenecid-Free and Wash-Free Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

METTL5 stabilizes c-Myc by facilitating USP5 translation to reprogram glucose metabolism and promote hepatocellular carcinoma progression<\/a>
Authors: <\/strong>Xia, Peng and Zhang, Hao and Lu, Haofeng and Xu, Kequan and Jiang, Xiang and Jiang, Yuke and Gongye, Xiangdong and Chen, Zhang and Liu, Jie and Chen, Xi and others,
Journal: <\/strong>Cancer Communications (2023)<\/p>\n\n\n\n

Cancer-associated fibroblast-secreted miR-421 promotes pancreatic cancer by regulating the SIRT3\/H3K9Ac\/HIF-1$\\alpha$ axis<\/a>
Authors: <\/strong>Zhou, Bin and Lei, Jing-Hao and Wang, Qiang and Qu, Teng-Fei and Cha, Li-Chao and Zhan, Han-Xiang and Liu, Shang-Long and Hu, Xiao and Sun, Chuan-Dong and Guo, Wei-Dong and others,
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lncENST Suppress the Warburg Effect Regulating the Tumor Progress by the Nkx2-5\/ErbB2 Axis in Hepatocellular Carcinoma<\/a>
Authors: <\/strong>Chen, Geng and Jiang, Jiayun and Wang, Xiaofei and Feng, Kai and Ma, Kuansheng
Journal: <\/strong>Computational and Mathematical Methods in Medicine (2021)<\/p>\n\n\n\n

Impaired glucocorticoid receptor expression in liver disrupts feeding-induced gene expression, glucose uptake, and glycogen storage<\/a>
Authors: <\/strong>Pr{\\ae}stholm, Stine M and Correia, Catarina M and Goitea, Victor E and Siersb{\\ae}k, Majken S and J{\\o}rgensen, Mathilde and Havelund, Jesper F and Pedersen, Thomas {\\AA} and F{\\ae}rgeman, Nils J and Gr{\\o}ntved, Lars
Journal: <\/strong>Cell reports (2021): 109938<\/p>\n\n\n\n

Short-Chain Hydroxyl CoA Dehydrogenase Probably is the Central Player in DNA Replication and Glucose Consumption in Human Non-Small Lung Carcinoma Cell Line<\/a>
Authors: <\/strong>Soleymaninejadian, Ehsan
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miR-143-3p functions as a tumor suppressor by targeting HK2 in neuroblastomas<\/a>
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Corticotropin releasing hormone can selectively stimulate glucose uptake in corticotropinoma via glucose transporter 1
Authors: <\/strong>Lu, Jie and Montgomery, Blake K and Chatain, Gr{\\’e}goire P and Bugarini, Alejandro and Zhang, Qi and Wang, Xiang and Edwards, Nancy A and Ray-Chaudhury, Abhik and Merrill, Marsha J and Lonser, Russell R and others,
Journal: <\/strong>Molecular and cellular endocrinology (2018): 105–114<\/p>\n\n\n\n

Corticotropin releasing hormone can selectively stimulate glucose uptake in corticotropinoma via glucose transporter 1<\/a>
Authors: <\/strong>Lu, Jie and Montgomery, Blake K and Chatain, Gr&eacute;goire P and Bugarini, Alej and ro , undefined and Zhang, Qi and Wang, Xiang and Edwards, Nancy A and Ray-Chaudhury, Abhik and Merrill, Marsha J and Lonser, Russell R and others, undefined
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A Non-Radioactive Enzymatic Photometric Assay for Glucose Uptake in Insulin-Responsive 3T3-L1 Adipocytes<\/a>
Authors: <\/strong>Zhao, Qin and Liao, Jinfang and Diwu, Zhenjun
Journal: <\/strong>Biophysical Journal (2014): 369a<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 115 referencias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Inhibition of the sodium glucose co-transporter-2: its beneficial action and potential combination therapy for type 2 diabetes mellitus
Authors: <\/strong>Chen LH, Leung PS.
Journal: <\/strong>Diabetes Obes Metab. (2013)<\/p>\n\n\n\n

Hepatic expression and cellular distribution of the glucose transporter family
Authors: <\/strong>Karim S, Adams DH, Lalor PF.
Journal: <\/strong>World J Gastroenterol (2012): 6771<\/p>\n\n\n\n

Sodium glucose co-transporter 2 (SGLT2) inhibitors: novel antidiabetic agents
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Journal: <\/strong>Expert Opin Ther Pat (2012): 483<\/p>\n\n\n\n

Research progress of sodium-glucose co-transporter 2 inhibitors for treatment of type 2 diabetes
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Journal: <\/strong>Yao Xue Xue Bao (2012): 716<\/p>\n\n\n\n

A new class of drug for the management of type 2 diabetes: sodium glucose co-transporter inhibitors: ‘glucuretics’
Authors: <\/strong>Chan HW, Ashan B, Jayasekera P, Collier A, Ghosh S.
Journal: <\/strong>Diabetes Metab Syndr (2012): 224<\/p>\n\n\n\n

Glucose transporter type I deficiency syndrome: epilepsy phenotypes and outcomes
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New possibility in the oral glucose lowering treatment of type 2 diabetes mellitus: sodium-glucose co-transporter-2 inhibitors
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Regulation of glucose transporter translocation in health and diabetes
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Sodium-dependent glucose transporter protein as a potential therapeutic target for improving glycemic control in diabetes
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Journal: <\/strong>Nutr Rev (2011): 720<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Non-Radioactive Photometric Assay for Glucose Uptake in Insulin-Responsive 3T3-L1 Adipocytes<\/a>
A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a>
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets<\/a>
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise (en Ingles)<\/mark><\/p>\n\n\n\n

Calbryte\u2122 series now available<\/a>
Hydroxyl Radical Detection<\/a>
Nucleic Acid Detection, Quantification and Imaging<\/a>
A practical guide for use of PE and APC in flow cytometry<\/a>
Fluorescent Phalloidin: A Practical Stain for Visualizing Actin Filaments<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Kit de ensayo colorim\u00e9trico de captaci\u00f3n de glucosa Screen Quest\u2122<\/p>\n","protected":false},"featured_media":4532,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4529"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":4,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4529\/revisions"}],"predecessor-version":[{"id":7805,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4529\/revisions\/7805"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/4532"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=4529"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=4529"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}