{"id":4511,"date":"2023-02-04T19:11:17","date_gmt":"2023-02-05T01:11:17","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=4511"},"modified":"2023-02-04T21:07:36","modified_gmt":"2023-02-05T03:07:36","slug":"screen-quest-colorimetric-chloride-channel-assay-kit","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/screen-quest-colorimetric-chloride-channel-assay-kit\/","title":{"rendered":"Screen Quest\u2122 Colorimetric Chloride Channel Assay Kit"},"content":{"rendered":"\n

Los canales de cloruro tienen una variedad de importantes funciones fisiol\u00f3gicas y celulares que incluyen la regulaci\u00f3n del pH, la homeostasis del volumen, el transporte de solutos org\u00e1nicos, la migraci\u00f3n celular, la proliferaci\u00f3n y diferenciaci\u00f3n celular. Los canales de cloruro representan valiosos objetivos farmacol\u00f3gicos. Varios estados de enfermedades cr\u00f3nicas, como la fibrosis qu\u00edstica y el s\u00edndrome de Bartter, se deben a defectos en las funciones de los canales de cloruro. <\/p>\n\n\n\n

Sin embargo, las tecnolog\u00edas existentes para la detecci\u00f3n de moduladores de los canales de cloruro son un compromiso entre el rendimiento, la sensibilidad y la relevancia fisiol\u00f3gica. El kit de ensayo colorim\u00e9trico de canales de cloruro Screen Quest\u2122 proporciona un m\u00e9todo colorim\u00e9trico sensible y s\u00f3lido para estudiar los canales de cloruro. <\/p>\n\n\n\n

El ensayo se basa en nuestro indicador de yoduro patentado (Iodide Blue\u2122) para medir la concentraci\u00f3n de yoduro, este ensayo detect\u00f3 tan solo 30 nM de yoduro. El kit de ensayo de canales de cloruro Screen Quest\u2122 proporciona un m\u00e9todo de ensayo optimizado para monitorear los canales de cloruro. <\/p>\n\n\n\n

El ensayo se puede realizar en un conveniente formato de placa de microtitulaci\u00f3n de 96 pocillos o de 384 pocillos y se puede adaptar f\u00e1cilmente a la automatizaci\u00f3n.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-36350<\/td>Screen Quest\u2122 Colorimetric Chloride Channel Assay Kit<\/td>10 placas<\/td><\/tr>
AAT-36351<\/td>Screen Quest\u2122 Colorimetric Chloride Channel Assay Kit<\/td>100 placas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Absorbancia<\/mark> <\/p>\n\n\n\n

Absorbancia<\/td>630, 380, o 405 nm<\/td><\/tr>
Placa recomendada<\/td>Fondo claro<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Componentes<\/mark><\/p>\n\n\n\n

Componente A: Iodide Blue\u2122 Sensor<\/td>1 botella (50 mL)<\/td><\/tr>
Componente B: Iodide Sensor Enhancer (100X)<\/td>1 vial (0.5 mL)<\/td><\/tr>
Componente C: Loading Buffer<\/td>1 botella (100 mL)<\/td><\/tr>
Componente D: Cell Lysis Buffer (10X)<\/td>1 botella (5 mL)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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PREPARACION CELULAR<\/p>\n\n\n\n

Para obtener guias sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite: https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n<\/div><\/div>\n<\/div><\/div>\n\n\n\n

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PREPARACION DE SOLUCION DE STOCK<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em> <\/p>\n\n\n\n

1. Buffer de lisis celular (1X) <\/em><\/p>\n\n\n\n

Agregue el vial completo de buffer de lisis celular 10X (componente D) a 45 ml de H2O est\u00e9ril y mezcle bien. Tenga en cuenta que 5 ml de buffer de lisis celular 1X es suficiente para una placa. Guarde el buffer de lisis celular 1X no utilizado a 4\u00b0C.<\/p>\n\n\n\n

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PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

Soluci\u00f3n Iodide Sensor Enhancer (1X) <\/em><\/p>\n\n\n\n

Agregue 50 \u00b5l de potenciador de sensor de yoduro 100X (componente B) a 5 ml de H2O est\u00e9ril y mezcle bien. Nota: <\/strong>La soluci\u00f3n 1X I- Sensor Enhancer no es estable; usar dentro de las 2 horas posteriores a la diluci\u00f3n. Nota:<\/strong> Cada l\u00ednea celular debe evaluarse individualmente para determinar la diluci\u00f3n \u00f3ptima de la soluci\u00f3n I-Sensor Enhancer. Observamos que la soluci\u00f3n 0.1X I- Sensor Enhancer funciona incluso mejor para algunas l\u00edneas celulares.<\/p>\n\n\n\n

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Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1. <\/strong>La respuesta a la dosis de NaI se midi\u00f3 con el kit de ensayo de canal de cloruro colorim\u00e9trico Screen Quest\u2122 en una placa transparente de 96 pocillos.<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Screen Quest\u2122 Membrane Potential Assay Kit *Orange <\/a>Fluorescence*<\/a><\/td><\/tr>
Screen Quest\u2122 Membrane Potential Assay Kit *Red Fluorescence*<\/a><\/td><\/tr>
Screen Quest\u2122 10X cell staining buffer with Phenol Red Plus\u2122<\/a><\/td><\/tr>
Screen Quest\u2122 10X calcium assay buffer with Phenol Red Plus\u2122<\/a><\/td><\/tr>
Screen Quest\u2122 Luminometric Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluo-8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines*<\/a><\/td><\/tr>
Screen Quest\u2122 Fluo-8 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fura-2 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Rhod-4 No Wash Calcium Assay Kit *Medium Removal*<\/a><\/td><\/tr>
Screen Quest\u2122 Rhod-4 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric MDR Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Colorimetric ELISA cAMP Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric ELISA cAMP Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 TR-FRET No Wash cAMP Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Live Cell cAMP Assay Service Pack<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric Fatty Acid Uptake Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric Glucose Uptake Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Colorimetric Glucose Uptake Assay Kit<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 44 referencias:\u00a0Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Synthesis of 4-thiophen-2′-yl-1,4-dihydropyridines as potentiators of the CFTR chloride channel
Authors: <\/strong>Cateni F, Zacchigna M, Pedemonte N, Galietta LJ, Mazzei MT, Fossa P, Giampieri M, Mazzei M.
Journal: <\/strong>Bioorg Med Chem (2009): 7894<\/p>\n\n\n\n

Genetically encoded optical sensors for monitoring of intracellular chloride and chloride-selective channel activity
Authors: <\/strong>Bregestovski P, Waseem T, Mukhtarov M.
Journal: <\/strong>Front Mol Neurosci (2009): 15<\/p>\n\n\n\n

Chloride intracellular channel protein-4 functions in angiogenesis by supporting acidification of vacuoles along the intracellular tubulogenic pathway
Authors: <\/strong>Ulmasov B, Bruno J, Gordon N, Hartnett ME, Edwards JC.
Journal: <\/strong>Am J Pathol (2009): 1084<\/p>\n\n\n\n

Statins and fenofibrate affect skeletal muscle chloride conductance in rats by differently impairing ClC-1 channel regulation and expression
Authors: <\/strong>Pierno S, Camerino GM, Cippone V, Roll and JF, Desaphy JF, De Luca A, Liantonio A, Bianco G, Kunic JD, George AL, Jr., Conte Camerino D.
Journal: <\/strong>Br J Pharmacol (2009): 1206<\/p>\n\n\n\n

Spatiotemporal regulation of chloride intracellular channel protein CLIC4 by RhoA
Authors: <\/strong>Ponsioen B, van Zeijl L, Langeslag M, Berryman M, Littler D, Jalink K, Moolenaar WH.
Journal: <\/strong>Mol Biol Cell (2009): 4664<\/p>\n\n\n\n

Chloride intracellular channel-4 is a determinant of native collateral formation in skeletal muscle and brain
Authors: <\/strong>Chalothorn D, Zhang H, Smith JE, Edwards JC, Faber JE.
Journal: <\/strong>Circ Res (2009): 89<\/p>\n\n\n\n

Effects of Chinese herbal medicine combined with He-Ne laser on lipoperoxide and superoxide dismutase in chloasma patients
Authors: <\/strong>Wu YH, Li QL, Yang XW.
Journal: <\/strong>J Tradit Chin Med (2009): 163<\/p>\n\n\n\n

Chloride intracellular channel 1 regulates osteoblast differentiation
Authors: <\/strong>Yang JY, Jung JY, Cho SW, Choi HJ, Kim SW, Kim SY, Kim HJ, Jang CH, Lee MG, Han J, Shin CS.
Journal: <\/strong>Bone (2009): 1175<\/p>\n\n\n\n

Two novel CLCN2 mutations accelerating chloride channel deactivation are associated with idiopathic generalized epilepsy
Authors: <\/strong>Saint-Martin C, Gauvain G, Teodorescu G, Gourfinkel-An I, Fedirko E, Weber YG, Maljevic S, Ernst JP, Garcia-Olivares J, Fahlke C, Nabbout R, LeGuern E, Lerche H, Poncer JC, Depienne C.
Journal: <\/strong>Hum Mutat (2009): 397<\/p>\n\n\n\n

Identification of metastasis-associated proteins involved in gallbladder carcinoma metastasis by proteomic analysis and functional exploration of chloride intracellular channel 1
Authors: <\/strong>Wang JW, Peng SY, Li JT, Wang Y, Zhang ZP, Cheng Y, Cheng DQ, Weng WH, Wu XS, Fei XZ, Quan ZW, Li JY, Li SG, Liu YB.
Journal: <\/strong>Cancer Lett (2009): 71<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a>
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets<\/a>
A Non-Radioactive Photometric Assay for Glucose Uptake in Insulin-Responsive 3T3-L1 Adipocytes<\/a>
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

AssayWise (en Ingles)<\/mark><\/p>\n\n\n\n

Calbryte\u2122 series now available<\/a>
Hydroxyl Radical Detection<\/a>
Nucleic Acid Detection, Quantification and Imaging<\/a>
A practical guide for use of PE and APC in flow cytometry<\/a>
Calbryte\u2122 series now available<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Este kit de ensayo de canal de cloruro colorim\u00e9trico Screen Quest\u2122 proporciona un m\u00e9todo colorim\u00e9trico sensible y s\u00f3lido para estudiar los canales de cloruro. <\/p>\n","protected":false},"featured_media":4512,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4511"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4511\/revisions"}],"predecessor-version":[{"id":4514,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4511\/revisions\/4514"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/4512"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=4511"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=4511"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}