{"id":4093,"date":"2023-02-01T11:57:08","date_gmt":"2023-02-01T17:57:08","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=4093"},"modified":"2023-02-01T12:18:52","modified_gmt":"2023-02-01T18:18:52","slug":"phosphoworks-luminometric-atp-assay-kit-steady-glow","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/phosphoworks-luminometric-atp-assay-kit-steady-glow\/","title":{"rendered":"PhosphoWorks\u2122 Luminometric ATP Assay Kit *Steady Glow*"},"content":{"rendered":"\n

El trifosfato de adenosina (ATP) juega un papel fundamental en la energenia celular, la regulaci\u00f3n metab\u00f3lica y la se\u00f1alizaci\u00f3n celular. <\/p>\n\n\n\n

El PhosphoWorks\u2122 ATP Assay Kit proporciona un ensayo de luminiscencia r\u00e1pido, simple y homog\u00e9neo para la determinaci\u00f3n de la proliferaci\u00f3n celular y la citotoxicidad en c\u00e9lulas de mam\u00edferos. <\/p>\n\n\n\n

El ensayo se puede realizar en un conveniente formato de placa de microtitulaci\u00f3n de 96 pocillos y 384 pocillos. La alta sensibilidad de este ensayo permite la detecci\u00f3n de ATP en muchos sistemas biol\u00f3gicos, muestras ambientales y alimentos. Este kit de ensayo PhosphoWorks ATP tiene una se\u00f1al de luminiscencia estable de hasta 4 horas. Tiene una luminiscencia estable sin necesidad de mezclas ni separaciones, y est\u00e1 formulado para tener un tiempo m\u00ednimo de manipulaci\u00f3n.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-21608<\/td>PhosphoWorks\u2122 Luminometric ATP Assay Kit *Steady Glow*<\/td>10 placas<\/td><\/tr>
AAT-21609<\/td>PhosphoWorks\u2122 Luminometric ATP Assay Kit *Steady Glow*<\/td>1 placa<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

\"pdf\"SDS<\/a>\"pdf\"Protocol<\/a><\/p>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Fluorescencia<\/mark> <\/p>\n\n\n\n

Placa recomendada<\/td>Blanca s\u00f3lida <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: ATP Monitoring Enzyme<\/td>1 vial<\/td><\/tr>
Componente B: ATP Sensor (Light-sensitive)<\/td>10 viales <\/td><\/tr>
Componente C: Buffer de reacci\u00f3n<\/td>2 viales (50 mL\/vial)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\n\n\n\n

PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

  1. Transfiera todo el vial de 10 ml de buffer de reacci\u00f3n (componente C) al sensor de ATP (componente B) y mezcle bien.<\/li>
  2. Agregue 20 \u00b5L de Enzima de Monitoreo de ATP (Componente A) en la botella de Componente B+C y mezcle bien para hacer una soluci\u00f3n de trabajo de ATP. Nota: Evite la posible contaminaci\u00f3n por ATP de fuentes biol\u00f3gicas ex\u00f3genas.<\/li><\/ol>\n\n\n\n

    <\/p>\n\n\n\n

    Para obtener guias sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite<\/p>\n\n\n\n

    https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n\n\n\n

    <\/p>\n<\/div><\/div>\n\n\n\n

    <\/div>\n\n\n\n

    Imagenes<\/mark><\/p>\n\n\n\n

    \"\"
    Fig. 1<\/figcaption><\/figure>\n\n\n\n

    Figura 1. <\/strong>El n\u00famero de c\u00e9lulas CHO-K1 se midi\u00f3 con el kit de ensayo de ATP de luminiscencia PhosphoWorks\u2122 en una placa blanca de 96 pocillos utilizando un lector de placas NOVOstar (BMG Labtech). La se\u00f1al de luminiscencia de las c\u00e9lulas CHO-K1 hasta 100 c\u00e9lulas por pocillo se control\u00f3 durante un m\u00e1ximo de 2 horas (factor Z’ = 0,6). El tiempo integrado fue de 1 segundo.<\/p>\n\n\n\n

    <\/div>\n\n\n\n
    \"\"
    Fig. 2<\/figcaption><\/figure>\n\n\n\n

    Figura 2.<\/strong> La respuesta a la dosis de ATP se midi\u00f3 con el kit de ensayo PhosphoWorks Luminescence ATP. Se controlaron las concentraciones de ATP de 10 uM a 0,1 nM durante un m\u00e1ximo de 5 horas a intervalos de 1 segundo.<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Productos Relacionados<\/mark><\/p>\n\n\n\n

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    <\/div>\n\n\n\n

    Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

    Coronary Endothelium No-Reflow Injury Is Associated with ROS-Modified Mitochondrial Fission through the JNK-Drp1 Signaling Pathway<\/a>
    Authors: <\/strong>Chen, Yi and Liu, Chen and Zhou, Peng and Li, Jiannan and Zhao, Xiaoxiao and Wang, Ying and Chen, Runzhen and Song, Li and Zhao, Hanjun and Yan, Hongbing
    Journal: <\/strong>Oxidative Medicine and Cellular Longevity (2021)<\/p>\n\n\n\n

    KD025 Shifts Pulmonary Endothelial Cell Bioenergetics and Decreases Baseline Lung Permeability<\/a>
    Authors: <\/strong>Lee, Ji Young and Stevens, Reece P and Kash, Mary and Zhou, Chun and Koloteva, Anna and Renema, Phoibe and Paudel, Sunita S and Stevens, Troy
    Journal: <\/strong>American Journal of Respiratory Cell and Molecular Biology (2020)<\/p>\n\n\n\n

    High throughput cell-based assay for identification of glycolate oxidase inhibitors as a potential treatment for Primary Hyperoxaluria Type 1<\/a>
    Authors: <\/strong>Wang, Mengqiao and Xu, Miao and Long, Yan and Fargue, Sonia and Southall, Noel and Hu, Xin and McKew, John C and Danpure, Christopher J and Zheng, Wei
    Journal: <\/strong>Scientific Reports (2016)<\/p>\n\n\n\n

    NT1014, a novel biguanide, inhibits ovarian cancer growth in vitro and in vivo<\/a>
    Authors: <\/strong>Zhang, Lu and Han, Jianjun and Jackson, Am and a L , undefined and Clark, Leslie N and Kilgore, Joshua and Guo, Hui and Livingston, Nick and Batchelor, Kenneth and Yin, Yajie and Gilliam, Timothy P and others, undefined
    Journal: <\/strong>Journal of Hematology & Oncology (2016): 91<\/p>\n\n\n\n

    The Different Effects of Atorvastatin and Pravastatin on Cell Death and PARP Activity in Pancreatic NIT-1 Cells<\/a>
    Authors: <\/strong>Chen, Ya-Hui and Chen, Yi-Chun and Liu, Chin-San and Hsieh, Ming-Chia
    Journal: <\/strong>Journal of Diabetes Research (2016)<\/p>\n\n\n\n

    Glutamine promotes ovarian cancer cell proliferation through the mTOR\/S6 pathway<\/a>
    Authors: <\/strong>Yuan, Lingqin and Sheng, Xiugui and Willson, Adam K and Roque, Dario R and Stine, Jessica E and Guo, Hui and Jones, Hannah M and Zhou, Chunxiao and Bae-Jump, Victoria L
    Journal: <\/strong>Endocrine-related cancer (2015): 577–591<\/p>\n\n\n\n

    BPA-induced DNA hypermethylation of the master mitochondrial gene PGC-1\u03b1 contributes to cardiomyopathy in male rats<\/a>
    Authors: <\/strong>Jiang, Ying and Xia, Wei and Yang, Jie and Zhu, Yingshuang and Chang, Huailong and Liu, Juan and Huo, Wenqian and Xu, Bing and Chen, Xi and Li, Yuanyuan and others, undefined
    Journal: <\/strong>Toxicology (2015): 21–31<\/p>\n\n\n\n

    Loss of histone deacetylase Hdac1 disrupts metabolic processes in intestinal epithelial cells<\/a>
    Authors: <\/strong>Gonneaud, Alexis and Turgeon, Naomie and Boisvert, Fran\u010dois-Michel and Boudreau, Fran\u010dois and Asselin, Claude
    Journal: <\/strong>FEBS letters (2015): 2776–2783<\/p>\n\n\n\n

    JQ1 suppresses tumor growth through downregulating LDHA in ovarian cancer<\/a>
    Authors: <\/strong>Qiu, Haifeng and Jackson, Am and a L , undefined and Kilgore, Joshua E and Zhong, Yan and Chan, Leo Li-Ying and Gehrig, Paola A and Zhou, Chunxiao and Bae-Jump, Victoria L
    Journal: <\/strong>Oncotarget (2015): 6915<\/p>\n\n\n\n

    A neutrophil intrinsic impairment affecting Rab27a and degranulation in cystic fibrosis is corrected by CFTR potentiator therapy<\/a>
    Authors: <\/strong>Pohl, Kerstin and Hayes, Elaine and Keenan, Joanne and Henry, Michael and Meleady, Paula and Molloy, Kevin and Jundi, Bakr and Bergin, David A and McCarthy, Cormac and McElvaney, Oliver J and others, undefined
    Journal: <\/strong>Blood (2014): 999–1009<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Referencias<\/mark><\/p>\n\n\n\n

    Ver todas las\u00a0108 referencias:\u00a0Citation Explorer<\/a><\/em><\/p>\n\n\n\n

    Cell-surface-localized ATP detection with immobilized firefly luciferase
    Authors: <\/strong>Nakamura M, Mie M, Funabashi H, Yamamoto K, Ando J, Kobatake E.
    Journal: <\/strong>Anal Biochem (2006): 61<\/p>\n\n\n\n

    Ca2+ oscillations stimulate an ATP increase during fertilization of mouse eggs
    Authors: <\/strong>Campbell K, Swann K.
    Journal: <\/strong>Dev Biol (2006): 225<\/p>\n\n\n\n

    An efficient method for quantitative determination of cellular ATP synthetic activity
    Authors: <\/strong>Hara KY, Mori H.
    Journal: <\/strong>J Biomol Screen (2006): 310<\/p>\n\n\n\n

    Basic evaluation for new antimicrobial susceptibility testing of Mycobacterium leprae by bioluminescence assay (ATP method)
    Authors: <\/strong>Yamazaki T, Gidoh M, Matsuoka M.
    Journal: <\/strong>Nihon Hansenbyo Gakkai Zasshi (2006): 227<\/p>\n\n\n\n

    Cells die with increased cytosolic ATP during apoptosis: a bioluminescence study with intracellular luciferase
    Authors: <\/strong>Zamaraeva MV, Sabirov RZ, Maeno E, Ando-Akatsuka Y, Bessonova SV, Okada Y.
    Journal: <\/strong>Cell Death Differ (2005): 1390<\/p>\n\n\n\n

    Do rat cardiac myocytes release ATP on contraction
    Authors: <\/strong>Godecke S, Stumpe T, Schiller H, Schnittler HJ, Schrader J.
    Journal: <\/strong>Am J Physiol Cell Physiol (2005): C609<\/p>\n\n\n\n

    ATP bioluminescence assay for estimation of microbial populations of fresh-cut melon
    Authors: <\/strong>Ukuku DO, Sapers GM, Fett WF.
    Journal: <\/strong>J Food Prot (2005): 2427<\/p>\n\n\n\n

    Determination of ATP impurity in adenine dinucleotides
    Authors: <\/strong>Pojoga LH, Haghiac ML, Moose JE, Hilderman RH.
    Journal: <\/strong>Nucleosides Nucleotides Nucleic Acids (2004): 581<\/p>\n\n\n\n

    Identification of kinase inhibitors by an ATP depletion method
    Authors: <\/strong>Singh P, Harden BJ, Lillywhite BJ, Broad PM.
    Journal: <\/strong>Assay Drug Dev Technol (2004): 161<\/p>\n\n\n\n

    ATP amplification for ultrasensitive bioluminescence assay: detection of a single bacterial cell
    Authors: <\/strong>Satoh T, Kato J, Takiguchi N, Ohtake H, Kuroda A.
    Journal: <\/strong>Biosci Biotechnol Biochem (2004): 1216<\/p>\n\n\n\n

    <\/div>\n\n\n\n

    Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

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    Bio-inspired NADH regeneration by carbon nitride photocatalysis using diatom templates<\/a><\/p>\n\n\n\n

    <\/div>\n\n\n\n
    <\/div>\n","protected":false},"excerpt":{"rendered":"

    Kit de ensayo de ATP luminom\u00e9trico PhosphoWorks\u2122 *Resplandor continuo*, proporciona un ensayo de luminiscencia r\u00e1pido, simple y homog\u00e9neo para la determinaci\u00f3n de la proliferaci\u00f3n celular y la citotoxicidad en c\u00e9lulas de mam\u00edferos. <\/p>\n","protected":false},"featured_media":4096,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4093"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4093\/revisions"}],"predecessor-version":[{"id":4098,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4093\/revisions\/4098"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/4096"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=4093"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=4093"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}