{"id":4068,"date":"2023-01-31T16:38:53","date_gmt":"2023-01-31T22:38:53","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=4068"},"modified":"2023-01-31T17:21:36","modified_gmt":"2023-01-31T23:21:36","slug":"cell-meter-multiplexing-caspase-3-7-8-and-9-activity-assay-kit-triple-fluorescence-colors-2","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cell-meter-multiplexing-caspase-3-7-8-and-9-activity-assay-kit-triple-fluorescence-colors-2\/","title":{"rendered":"Cell Meter\u2122 Multiplexing Caspase 3\/7, 8 and 9 Activity Assay Kit *Triple Fluorescence Colors*"},"content":{"rendered":"\n

Nuestros kits de ensayo Cell Meter\u2122 son un conjunto de herramientas para monitorear la viabilidad celular. Hay una variedad de par\u00e1metros que se pueden usar para monitorear la viabilidad celular. <\/p>\n\n\n\n

La activaci\u00f3n de caspasas es ampliamente aceptada como un indicador fiable de la apoptosis celular. Este kit en particular est\u00e1 dise\u00f1ado para monitorear simult\u00e1neamente la activaci\u00f3n de cuatro caspasas clave (caspasa-3\/7, 8 y 9) involucradas en la apoptosis celular usando tres colores fluorescentes distintos. Utiliza DEVD-ProRed\u2122, IETD-R110 y LEHD-AMC como indicadores fluorog\u00e9nicos para la actividad de caspasa 3\/7, 8 y 9 respectivamente. Tras la escisi\u00f3n de caspasa, los sustratos de caspasa DEVD-ProRed\u2122, IETD-R110 y LEHD-AMC generan tres fluor\u00f3foros distintos: ProRed\u2122 (fluorescencia roja), R110 (fluorescencia verde) y AMC (fluorescencia azul), que se pueden monitorear f\u00e1cilmente en un solo ensayo debido a su buena separaci\u00f3n espectral.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22820<\/td>Cell Meter\u2122 Multiplexing Caspase 3\/7, 8 and 9 Activity Assay Kit <\/td>100 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Fluorescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>Ver Tabla 1<\/td><\/tr>
Emisi\u00f3n<\/td>Ver Tabla 1<\/td><\/tr>
Placa recomendada <\/td>Pared negra\/Fondo transparente<\/td><\/tr>
Especificaciones Instrumento<\/td>Modo de lectura inferior o superior<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Tabla 1<\/mark><\/p>\n\n\n\n

Longitudes de onda espectrales para Caspases 3\/7, 8, y 9.<\/p>\n\n\n\n

Caspase<\/strong><\/td>Fluorescence<\/strong><\/td>Excitation<\/strong><\/td>Emission<\/strong><\/td><\/tr>
Caspase 3\/7<\/td>Red<\/td>535 nm<\/td>620 nm<\/td><\/tr>
Caspase 8<\/td>Green<\/td>490 nm<\/td>525 nm<\/td><\/tr>
Caspase 9<\/td>Blue<\/td>370 nm<\/td>450 nm<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: Caspase 3\/7 Substrate (DEVD-ProRed\u2122, 200X )<\/td>1 vial (50 \u00b5L\/vial)<\/td><\/tr>
Componente B: Caspase 8 Substrate (IETD-R110, 200X)<\/td>1 vial (50 \u00b5L\/vial)<\/td><\/tr>
Componente C: Caspase 9 Substrate (LEHD-AMC, 200X)<\/td>1 vial (50 \u00b5L\/vial)<\/td><\/tr>
Componente D: Buffer de ensayo<\/td>1 botella (30 mL)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n de soluci\u00f3n de trabajo<\/p>\n\n\n\n

  1. Soluci\u00f3n de trabajo de actividad de caspasa \u00fanica<\/em>
    Prepare una soluci\u00f3n de trabajo de caspasa 3\/7, caspasa 8 o caspasa 9 agregando 50 \u00b5l de sustrato (componente A, B o C) en 10 ml de buffer de ensayo (componente D) y m\u00e9zclelos bien.<\/li><\/ol>\n\n\n\n
    1. Soluci\u00f3n de trabajo de actividad dual o tricaspasa:<\/em>
      Agregue 50 \u00b5l de cada sustrato de caspasa interesado en 10 ml de buffer de ensayo (componente D) para formar la soluci\u00f3n de trabajo. Nota: Prepare las soluciones de sustrato probadas y el volumen necesario proporcionalmente.<\/li><\/ol>\n\n\n\n

      <\/p>\n\n\n\n

      Para conocer guias sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite: https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n<\/div><\/div>\n\n\n\n

      <\/div>\n\n\n\n

      Imagenes<\/mark><\/p>\n\n\n\n

      \"\"
      Fig. 1<\/figcaption><\/figure>\n\n\n\n

      Figura 1.<\/strong> Detecci\u00f3n de actividades de caspasa en c\u00e9lulas Jurkat. Se sembraron c\u00e9lulas Jurkat el mismo d\u00eda a raz\u00f3n de 200.000 c\u00e9lulas\/pocillo en una placa de 96 pocillos de pared negra\/fondo transparente. Las c\u00e9lulas se trataron con estaurosporina a la concentraci\u00f3n final de 1 mM durante 4 horas (barra roja), mientras que las c\u00e9lulas no tratadas se usaron como control (barra azul). Se a\u00f1adi\u00f3 la soluci\u00f3n de carga de ensayo de caspasa \u00fanica (100 uL\/pocillo) (en el n.\u00b0 1 para caspasa 3\/7, n.\u00b0 2 para caspasa 8 o n.\u00b0 3 para caspasa 9) o soluci\u00f3n de carga de ensayo de triple caspasa (n.\u00b0 4 para caspasa 3 \/7, 8 y 9 juntos) y se incub\u00f3 a temperatura ambiente durante 1 hora. La intensidad de la fluorescencia se midi\u00f3 con el lector de microplacas de fluorescencia FlexStation a la longitud de onda indicada. Las actividades de las caspasas 3\/7, 8 y 9 se pueden detectar en un solo ensayo sin interferencias de otras caspasas.<\/p>\n\n\n\n

      <\/div>\n\n\n\n
      \"\"
      Fig. 2<\/figcaption><\/figure>\n\n\n\n

      Figura 2.<\/strong> Apoptosis inducida por cSBL en c\u00e9lulas H2452 y MSTO mediante la activaci\u00f3n de la v\u00eda de la caspasa. La activaci\u00f3n de caspasa-3, -8 y -9 se detect\u00f3 mediante transferencia Western (A, B) o fluorometr\u00eda (C, D). La fluorometr\u00eda se realiz\u00f3 de forma independiente tres veces y los datos se expresan como la media \u00b1 SD. La significaci\u00f3n estad\u00edstica de estos experimentos en comparaci\u00f3n con el control se muestra a continuaci\u00f3n: *P<0,05, **P<0,01, ***P<0,001. Fuente: La lectina que se une al \u00e1cido si\u00e1lico de los huevos de rana toro inhibe el crecimiento de c\u00e9lulas de mesotelioma maligno humano in vitro e in vivo por Tatsuta T et al., PLOS, enero de 2018.<\/p>\n\n\n\n

      <\/div>\n\n\n\n
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      <\/div>\n\n\n\n

      Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

      Ver todas las 23 bibliograf\u00edas: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

      Asparagus officinalis combined with paclitaxel exhibited synergistic anti-tumor activity in paclitaxel-sensitive and-resistant ovarian cancer cells<\/a>
      Authors: <\/strong>Zhang, Xin and Wang, Jiandong and Fan, Yali and Zhao, Ziyi and Paraghamian, Sarah E and Hawkins, Gabrielle M and Buckingham, Lindsey and O\u2019Donnell, Jillian and Hao, Tianran and Suo, Hongyan and others,
      Journal: <\/strong>Journal of Cancer Research and Clinical Oncology (2022): 1–13<\/p>\n\n\n\n

      An{\\’a}lise da estrutura tridimensional, especificidade e atividade antic{\\^a}ncer da lectina Canavalia villosa (Benth)<\/a>
      Authors: <\/strong>L{\\’o}ssio, Cl{\\’a}udia Figueiredo
      Journal: <\/strong>(2021)<\/p>\n\n\n\n

      Changes in Apoptotic Pathways in MOLM-13 Cell Lines after Induction of Resistance to Hypomethylating Agents<\/a>
      Authors: <\/strong>Janotka, L’ubo{\\v{s}} and Messingerov{\\’a}, Lucia and {\\v{S}}imoni{\\v{c}}ov{\\’a}, Krist{\\’\\i}na and Kavcov{\\’a}, Helena and Elefantov{\\’a}, Katar{\\’\\i}na and Sulov{\\’a}, Zdena and Breier, Albert
      Journal: <\/strong>International journal of molecular sciences (2021): 2076<\/p>\n\n\n\n

      Hypertonicity-enforced BCL-2 addiction unleashes the cytotoxic potential of death receptors
      Authors: <\/strong>Sirtl, Simon and Knoll, Gertrud and Trinh, Dieu Thuy and Lang, Isabell and Siegmund, Daniela and Gross, Stefanie and Schuler-Thurner, Beatrice and Neubert, Patrick and Jantsch, Jonathan and Wajant, Harald and others,
      Journal: <\/strong>Oncogene (2018): 4122–4136<\/p>\n\n\n\n

      Hypertonicity-imposed BCL-XL addiction primes colorectal cancer cells for death<\/a>
      Authors: <\/strong>Heimer, Sina and Knoll, Gertrud and Steixner, Charlotte and Calance, Diana Nicoleta and Trinh, Dieu Thuy and Ehrenschwender, Martin
      Journal: <\/strong>Cancer Letters (2018)<\/p>\n\n\n\n

      Sialic acid-binding lectin from bullfrog eggs inhibits human malignant mesothelioma cell growth in vitro and in vivo<\/a>
      Authors: <\/strong>Tatsuta, Takeo and Satoh, Toshiyuki and Sugawara, Shigeki and Hara, Akiyoshi and Hosono, Masahiro
      Journal: <\/strong>PloS one (2018): e0190653<\/p>\n\n\n\n

      Role of lincRNA-p21 in the protective effect of macrophage inhibition factor against hypoxia\/serum deprivation-induced apoptosis in mesenchymal stem cells<\/a>
      Authors: <\/strong>Xia, Wenzheng and Zhuang, Lei and Hou, Meng
      Journal: <\/strong>International journal of molecular medicine (2018)<\/p>\n\n\n\n

      Helicobacter pylori secreted protein HP1286 triggers apoptosis in macrophages via TNF-independent and ERK MAPK-dependent pathways<\/a>
      Authors: <\/strong>Tavares, Raquel and Pathak, Sushil Kumar
      Journal: <\/strong>Frontiers in Cellular and Infection Microbiology (2017): 58<\/p>\n\n\n\n

      Hyperosmotic stress enhances cytotoxicity of SMAC mimetics
      Authors: <\/strong>Bittner, Sebastian and Knoll, Gertrud and Ehrenschwender, Martin
      Journal: <\/strong>Cell death \\& disease (2017): e2967–e2967<\/p>\n\n\n\n

      Promotion of osteointegration under diabetic conditions by tantalum coating-based surface modification on 3-dimensional printed porous titanium implants<\/a>
      Authors: <\/strong>Wang, Lin and Hu, Xiaofan and Ma, Xiangyu and Ma, Zhensheng and Zhang, Yang and Lu, Yizhao and Li, Xiang and Lei, Wei and Feng, Yafei
      Journal: <\/strong>Colloids and Surfaces B: Biointerfaces (2016): 440–452<\/p>\n\n\n\n

      <\/div>\n\n\n\n

      Application notes (en Ingles)<\/mark><\/p>\n\n\n\n

      Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
      Annexin V<\/a>
      Calcein<\/a>
      Human High Temperature Requirement Serine Protease A1 (HTRA1) Degrades Tau Protein Aggregates<\/a>
      Abbreviation of Common Chemical Compounds Related to Peptides<\/a><\/p>\n\n\n\n

      <\/div>\n","protected":false},"excerpt":{"rendered":"

      Kit de ensayo de actividad 3\/7, 8 y 9 de caspasa multiplexada Cell Meter\u2122 *Colores de fluorescencia triple*<\/p>\n","protected":false},"featured_media":3186,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4068"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4068\/revisions"}],"predecessor-version":[{"id":4073,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/4068\/revisions\/4073"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3186"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=4068"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=4068"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}