{"id":3999,"date":"2023-01-30T12:28:14","date_gmt":"2023-01-30T18:28:14","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3999"},"modified":"2023-01-30T12:42:40","modified_gmt":"2023-01-30T18:42:40","slug":"cell-navigator-mitochondrion-staining-kit-blue-fluorescence","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cell-navigator-mitochondrion-staining-kit-blue-fluorescence\/","title":{"rendered":"Cell Navigator\u00ae Mitochondrion Staining Kit *Blue Fluorescence*"},"content":{"rendered":"\n

Nuestros kits de im\u00e1genes de fluorescencia Cell Navigator\u00ae son un conjunto de herramientas de im\u00e1genes de fluorescencia para etiquetar org\u00e1nulos subcelulares como membranas, lisosomas, mitocondrias y n\u00facleos, etc. El etiquetado selectivo de compartimentos de c\u00e9lulas vivas proporciona un m\u00e9todo poderoso para estudiar eventos celulares en un espacio y contexto temporal. <\/p>\n\n\n\n

Este kit en particular est\u00e1 dise\u00f1ado para marcar mitocondrias de c\u00e9lulas vivas en fluorescencia azul. El kit utiliza un colorante patentado que se acumula selectivamente en las mitocondrias, probablemente a trav\u00e9s del gradiente de potencial de membrana mitocondrial. El indicador mitocondrial es un compuesto hidrof\u00f3bico que penetra f\u00e1cilmente en las c\u00e9lulas vivas intactas y queda atrapado en las mitocondrias despu\u00e9s de que ingresa a las c\u00e9lulas. Este indicador mitocondrial fluorescente se retiene en las mitocondrias durante mucho tiempo ya que el indicador lleva un grupo de retenci\u00f3n celular. Esta caracter\u00edstica clave aumenta significativamente su eficacia de tinci\u00f3n. El protocolo de etiquetado es s\u00f3lido y requiere un tiempo m\u00ednimo de manipulaci\u00f3n. Puede adaptarse f\u00e1cilmente a una amplia variedad de plataformas de fluorescencia, como ensayos en microplaca, inmunocitoqu\u00edmica y citometr\u00eda de flujo. Es \u00fatil para una variedad de estudios, incluida la adhesi\u00f3n celular, la quimiotaxis, la resistencia a m\u00faltiples f\u00e1rmacos, la viabilidad celular, la apoptosis y la citotoxicidad. <\/p>\n\n\n\n

El kit proporciona todos los componentes esenciales con un protocolo de etiquetado celular optimizado. Es adecuado para c\u00e9lulas proliferantes y no proliferativas, y se puede utilizar tanto para c\u00e9lulas en suspensi\u00f3n como adherentes.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22665<\/td>Cell Navigator\u00ae Mitochondrion Staining Kit *Blue Fluorescence*<\/td>500 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Microscopio de Fluorescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>Filtro DAPI <\/td><\/tr>
Emisi\u00f3n<\/td>Filtro DAPI <\/td><\/tr>
Placa recomendada <\/td>Pared negra \/fondo claro<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Componentes<\/mark><\/p>\n\n\n\n

Componente A: MitoLite\u2122 Blue<\/td>1 vial (100 \u00b5L, 500X DMSO solucion stock)<\/td><\/tr>
Componente B: Live Cell Staining Buffer<\/td>1 botella (50 mL)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Exitaci\u00f3n<\/td>344<\/td><\/tr>
Emisi\u00f3n<\/td>469<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n de soluci\u00f3n de trabajo<\/p>\n\n\n\n

Agregue 20 \u00b5l de 500X Mitolite\u2122 Blue (componente A) en 10 ml de buffer de tinci\u00f3n de c\u00e9lulas vivas (componente B) para preparar la soluci\u00f3n de trabajo de Mitolite\u2122 Blue. Proteger de la luz. <\/p>\n\n\n\n

Nota: <\/strong>20 \u00b5L de 500X Mitolite\u2122 Blue (Componente A) es suficiente para una placa de 96 pocillos. La concentraci\u00f3n \u00f3ptima del indicador mitocondrial fluorescente var\u00eda seg\u00fan la aplicaci\u00f3n espec\u00edfica. Las condiciones de tinci\u00f3n pueden modificarse seg\u00fan el tipo de c\u00e9lula particular y la permeabilidad de las c\u00e9lulas o tejidos a la sonda. <\/p>\n\n\n\n

Para conocer guias sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite: https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n<\/div><\/div>\n\n\n\n

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Imagen<\/mark><\/p>\n\n\n\n

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Figura 1.<\/strong> Imagen de c\u00e9lulas HeLa te\u00f1idas con el kit de tinci\u00f3n mitocondrial Cell Navigator\u00ae Blue Fluorescence<\/em> en una placa de fondo transparente de 96 pocillos.<\/p>\n\n\n\n

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Formatos alternativos<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td><\/tr>
Cell Navigator\u00ae Mitochondrion Staining Kit *Green Fluorescence*<\/a><\/td>508<\/td>528<\/td><\/tr>
Cell Navigator\u00ae Mitochondrion Staining Kit *Orange Fluorescence*<\/a><\/td>553<\/td>576<\/td><\/tr>
Cell Navigator\u00ae Mitochondrion Staining Kit *Red Fluorescence*<\/a><\/td>580<\/td>598<\/td><\/tr>
Cell Navigator\u00ae Mitochondrion Staining Kit *Deep Red Fluorescence*<\/a><\/td>640<\/td>659<\/td><\/tr>
Cell Navigator\u00ae Mitochondrion Staining Kit *NIR Fluorescence*<\/a><\/td>658<\/td>691<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Productos Relacionados<\/mark><\/p>\n\n\n\n

Cell Navigator\u00ae Live Cell RNA Imaging Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae Live Cell Endoplasmic Reticulum (ER) Staining Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae Live Cell Endoplasmic Reticulum (ER) Staining Kit *Red Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae F-Actin Labeling Kit *Blue Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae F-Actin Labeling Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae F-Actin Labeling Kit *Orange Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae F-Actin Labeling Kit *Red Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae Cell Plasma Membrane Staining Kit *Orange Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae Cell Plasma Membrane Staining Kit *Red Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae Fluorimetric Lipid Droplet Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae Fluorimetric Lipid Droplet Assay Kit *Red Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae NBD Ceramide Golgi Staining Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae Live Cell Tubulin Staining Kit<\/a><\/td><\/tr>
Cell Navigator\u00ae Cell Plasma Membrane Staining Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Navigator\u00ae Live Cell Endoplasmic Reticulum (ER) Staining Kit *Blue Fluorescence*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer<\/a>
Authors: <\/strong>Heger, Zbynek and Polanska, Hana and Krizkova, Sona and Balvan, Jan and Raudenska, Martina and Dostalova, Simona and Moulick, Amitava and Masarik, Michal and Adam, Vojtech
Journal: <\/strong>Colloids and Surfaces B: Biointerfaces (2017): 131–140<\/p>\n\n\n\n

Inhibition of heme oxygenase-1 enhances the chemosensitivity of laryngeal squamous cell cancer Hep-2 cells to cisplatin<\/a>
Authors: <\/strong>Lv, Xin and Song, Dong-mei and Niu, Ying-hao and Wang, Bao-shan
Journal: <\/strong>Apoptosis (2016): 489–501<\/p>\n\n\n\n

Effective two-photon excited photodynamic therapy of xenograft tumors sensitized by water-soluble bis (arylidene) cycloalkanone photosensitizers<\/a>
Authors: <\/strong>Zou, Qianli and Zhao, Hongyou and Zhao, Yuxia and Fang, Yanyan and Chen, Defu and Ren, Jie and Wang, Xiaopu and Wang, Ying and Gu, Ying and Wu, Feipeng
Journal: <\/strong>Journal of medicinal chemistry (2015): 7949–7958<\/p>\n\n\n\n

Melatonin promotes adipogenesis and mitochondrial biogenesis in 3T3-L1 preadipocytes<\/a>
Authors: <\/strong>Kato, Hisashi and Tanaka, Goki and Masuda, Shinya and Ogasawara, Junetsu and Sakurai, Takuya and Kizaki, Takako and Ohno, Hideki and Izawa, Tetsuya
Journal: <\/strong>Journal of Pineal Research (2015): 267–275<\/p>\n\n\n\n

L’histone D{\\’e}sac{\\’e}tylase HDAC1 Influence la R{\\’e}ponse {\\`A} Des Stress M{\\’e}taboliques Dans Les Cellules {\\’E}pith{\\’e}liales Intestinales<\/a>
Authors: <\/strong>Gonneaud, Alexis
Journal: <\/strong>(2014)<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 70 referencias: Citation Explorer<\/a><\/em><\/p>\n\n\n\n

Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser-induced fluorescence detection
Authors: <\/strong>Feng J, Arriaga EA.
Journal: <\/strong>Electrophoresis (2008): 475<\/p>\n\n\n\n

Calcium, mitochondria and apoptosis studied by fluorescence measurements
Authors: <\/strong>Roy SS, Hajnoczky G.
Journal: <\/strong>Methods (2008): 213<\/p>\n\n\n\n

Fluorescence imaging of mitochondria in yeast
Authors: <\/strong>Swayne TC, Gay AC, Pon LA.
Journal: <\/strong>Methods Mol Biol (2007): 433<\/p>\n\n\n\n

A fluorescence assay for peptide translocation into mitochondria
Authors: <\/strong>Martinez-Caballero S, Peixoto PM, Kinnally KW, Campo ML.
Journal: <\/strong>Anal Biochem (2007): 76<\/p>\n\n\n\n

Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection
Authors: <\/strong>Duffy CF, MacCraith B, Diamond D, O’Kennedy R, Arriaga EA.
Journal: <\/strong>Lab Chip (2006): 1007<\/p>\n\n\n\n

Discrimination of depolarized from polarized mitochondria by confocal fluorescence resonance energy transfer
Authors: <\/strong>Elmore SP, Nishimura Y, Qian T, Herman B, Lemasters JJ.
Journal: <\/strong>Arch Biochem Biophys (2004): 145<\/p>\n\n\n\n

A fluorescence-based technique for screening compounds that protect against damage to brain mitochondria
Authors: <\/strong>Kristian T, Fiskum G.
Journal: <\/strong>Brain Res Brain Res Protoc (2004): 176<\/p>\n\n\n\n

Determination of the cardiolipin content of individual mitochondria by capillary electrophoresis with laser-induced fluorescence detection
Authors: <\/strong>Fuller KM, Duffy CF, Arriaga EA.
Journal: <\/strong>Electrophoresis (2002): 1571<\/p>\n\n\n\n

Fluorescence imaging of metabolic responses in single mitochondria
Authors: <\/strong>Nakayama S, Sakuyama T, Mitaku S, Ohta Y.
Journal: <\/strong>Biochem Biophys Res Commun (2002): 23<\/p>\n\n\n\n

Visualisation of mitochondria in living neurons with single- and two-photon fluorescence laser microscopy
Authors: <\/strong>Dedov VN, Cox GC, Roufogalis BD.
Journal: <\/strong>Micron (2001): 653<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
Calcein<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Este kit es \u00fatil para una variedad de estudios, incluida la adhesi\u00f3n celular, la quimiotaxis, la resistencia a m\u00faltiples f\u00e1rmacos, la viabilidad celular, la apoptosis y la citotoxicidad. <\/p>\n","protected":false},"featured_media":4003,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3999"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":2,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3999\/revisions"}],"predecessor-version":[{"id":4004,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3999\/revisions\/4004"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/4003"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3999"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3999"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}