{"id":3985,"date":"2023-01-28T22:11:42","date_gmt":"2023-01-29T04:11:42","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3985"},"modified":"2023-01-28T22:47:04","modified_gmt":"2023-01-29T04:47:04","slug":"mitolite-green-fm","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/mitolite-green-fm\/","title":{"rendered":"MitoLite\u2122 Green FM\u00a0"},"content":{"rendered":"\n

MitoLite\u2122 Green FM es la misma mol\u00e9cula que MitoTracker Green FM (M7514, ThermoFisher). Es una tinci\u00f3n mitocondrial verde-fluorescente. A diferencia de otras sondas MitoLite, MitoLite\u2122 Green FM parece localizarse en las mitocondrias, y mucho menos dependiendo del potencial de la membrana mitocondrial. El tinte ti\u00f1e las c\u00e9lulas vivas, pero no se retiene bien despu\u00e9s de la fijaci\u00f3n con aldeh\u00eddo.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22695<\/td>MitoLite\u2122 Green FM<\/td>10 x 50 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

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Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Cit\u00f3metro de Flujo<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n Laser<\/td>488 nm<\/td><\/tr>
Emisi\u00f3n Filtro<\/td>530\/30 nm <\/td><\/tr>
Especificaciones Instrumento <\/td>Canal FITC <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Microscopio de Fluorescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>Juego de filtros FITC <\/td><\/tr>
Emisi\u00f3n<\/td>Juego de filtros FITC <\/td><\/tr>
Placa recomendada <\/td>Pared negra \/fondo claro<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>671.88<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Exitaci\u00f3n<\/td>508<\/td><\/tr>
Emisi\u00f3n<\/td>528<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan <\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de MitoLite\u2122 Green FM a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/em><\/td>10 mg<\/em><\/td><\/tr>
1 mM<\/strong><\/td>148.836 \u00b5L<\/td>744.181 \u00b5L<\/td>1.488 mL<\/td>7.442 mL<\/td>14.884 mL<\/td><\/tr>
5 mM<\/strong><\/td>29.767 \u00b5L<\/td>148.836 \u00b5L<\/td>297.672 \u00b5L<\/td>1.488 mL<\/td>2.977 mL<\/td><\/tr>
10 mM<\/strong><\/td>14.884 \u00b5L<\/td>74.418 \u00b5L<\/td>148.836 \u00b5L<\/td>744.181 \u00b5L<\/td>1.488 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Preparaci\u00f3n de soluci\u00f3n de Stock <\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

Soluci\u00f3n de stock MitoLite\u2122 Green FM :
<\/em>Disuelva un vial de MitoLite\u2122 Green FM (50 ug) en 74 uL de dimetilsulf\u00f3xido anhidro (DMSO) de alta calidad para preparar una soluci\u00f3n madre de 1 mM. Nota: Mantenga la soluci\u00f3n madre congelada a \u2264\u201315 \u00b0C y protegida de la luz.<\/p>\n\n\n\n

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Preparaci\u00f3n de soluci\u00f3n de trabajo<\/p>\n\n\n\n

MitoLite\u2122 Green FM soluci\u00f3n de tenci\u00f3n:<\/em>
Diluya la soluci\u00f3n madre de MitoLite\u2122 Green FM 1 mM hasta la concentraci\u00f3n de trabajo final en el buffer HH. La concentraci\u00f3n de trabajo puede estar en el rango de 20 a 200 nM.<\/p>\n\n\n\n

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Imagen<\/mark><\/p>\n\n\n\n

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Figura 1.<\/p>\n\n\n\n

Im\u00e1genes de fluorescencia de c\u00e9lulas HeLa te\u00f1idas con MitoLite\u2122 Green FM utilizando un microscopio de fluorescencia con un juego de filtros FITC (verde). Las c\u00e9lulas vivas se ti\u00f1eron conjuntamente con colorante de lisosoma LysoBrite\u2122 Red (cat\u00e1logo 22645, rojo) y tinci\u00f3n de n\u00facleos Nuclear Violet\u2122 LCS1 (cat\u00e1logo 17543, azul).<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

MitoLite\u2122 Blue FX490<\/a><\/td><\/tr>
MitoLite\u2122 Orange FX570<\/a><\/td><\/tr>
MitoLite\u2122 Red FX600<\/a><\/td><\/tr>
MitoLite\u2122 Deep Red FX660<\/a><\/td><\/tr>
MitoLite\u2122 Orange EX405<\/a><\/td><\/tr>
MitoLite\u2122 NIR FX690<\/a><\/td><\/tr>
MitoLite\u2122 Red CMXRos<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Sonodynamic Therapy Promotes Efferocytosis via CD47 Down-Regulation in Advanced Atherosclerotic Plaque<\/a>
Authors: <\/strong>Cao, Yang and Yao, Jianting and Gao, Weiwei and Cao, Zhengyu and Diabakte, Kamal and Wang, Linxin and Sun, Xin and Tian, Ye
Journal: <\/strong>International Heart Journal (2022): 21–233<\/p>\n\n\n\n

CDK4\/6 blockade provides an alternative approach for treatment of mismatch-repair deficient tumors<\/a>
Authors: <\/strong>Salewski, Inken and Henne, Julia and Engster, Leonie and Krone, Paula and Schneider, Bjoern and Redwanz, Caterina and Lemcke, Heiko and Henze, Larissa and Junghanss, Christian and Maletzki, Claudia
Journal: <\/strong>Oncoimmunology (2022): 2094583<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

The influence of platelets and a comparison of analytical approaches
Authors: <\/strong>Hopkinson, K.; Williams, E. A.; Fairburn, B.; Forster, S.; Flower, D. J.; Saxton, J. M.; Pockley, A. G., A MitoTracker Green-based flow cytometric assay for natural killer cell activity: variability
Journal: <\/strong>Exp Hematol (2007): 350-7<\/p>\n\n\n\n

Efficacy of MitoTracker Green and CMXrosamine to measure changes in mitochondrial membrane potentials in living cells and tissues
Authors: <\/strong>Pendergrass, W.; Wolf, N.; Poot, M.
Journal: <\/strong>Cytometry A (2004): 162-9<\/p>\n\n\n\n

MitoTracker Green labeling of mitochondrial proteins and their subsequent analysis by capillary electrophoresis with laser-induced fluorescence detection
Authors: <\/strong>Presley, A. D.; Fuller, K. M.; Arriaga, E. A.
Journal: <\/strong>J Chromatogr B Analyt Technol Biomed Life Sci (2003): 141-50<\/p>\n\n\n\n

Mitotracker green is a P-glycoprotein substrate
Authors: <\/strong>Marques-Santos, L. F.; Oliveira, J. G.; Maia, R. C.; Rumjanek, V. M.
Journal: <\/strong>Biosci Rep (2003): 199-212<\/p>\n\n\n\n

and MitoTracker Green is affected by mitochondrial membrane potential altering drugs
Authors: <\/strong>Keij, J. F.; Bell-Prince, C.; Steinkamp, J. A., Staining of mitochondrial membranes with 10-nonyl acridine orange, MitoFluor Green
Journal: <\/strong>Cytometry (2000): 203-10<\/p>\n\n\n\n

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Application notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
Calcein<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

MitoLite\u2122 Green FM es una tinci\u00f3n mitocondrial verde-fluorescente. <\/p>\n","protected":false},"featured_media":3987,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3985"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3985\/revisions"}],"predecessor-version":[{"id":3990,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3985\/revisions\/3990"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3987"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3985"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3985"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}