{"id":3978,"date":"2023-01-28T21:45:28","date_gmt":"2023-01-29T03:45:28","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3978"},"modified":"2023-01-28T22:10:29","modified_gmt":"2023-01-29T04:10:29","slug":"mitolite-blue-fx490","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/mitolite-blue-fx490\/","title":{"rendered":"MitoLite\u2122 Blue FX490\u00a0"},"content":{"rendered":"\n

MitoLite\u2122 Blue es un colorante cati\u00f3nico que se acumula selectivamente en las mitocondrias, probablemente a trav\u00e9s del gradiente de potencial de la membrana mitocondrial. El indicador mitocondrial es un compuesto hidrof\u00f3bico que penetra f\u00e1cilmente en las c\u00e9lulas vivas intactas y queda atrapado en las mitocondrias despu\u00e9s de que ingresa a las c\u00e9lulas. <\/p>\n\n\n\n

Este indicador mitocondrial fluorescente se retiene en las mitocondrias durante mucho tiempo ya que el indicador lleva un grupo de retenci\u00f3n celular. Esta caracter\u00edstica clave aumenta significativamente su eficacia de tinci\u00f3n. El protocolo de etiquetado es s\u00f3lido y requiere un tiempo m\u00ednimo de manipulaci\u00f3n. Puede adaptarse f\u00e1cilmente a una amplia variedad de plataformas de fluorescencia, como ensayos en microplaca, inmunocitoqu\u00edmica y citometr\u00eda de flujo. <\/p>\n\n\n\n

Es adecuado para c\u00e9lulas proliferantes y no proliferativas, y se puede utilizar tanto para c\u00e9lulas en suspensi\u00f3n como adherentes.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22674<\/td>MitoLite\u2122 Blue FX490 <\/td>500 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

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Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelaci\u00f3n (< -15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>N\/D<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Ver en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Exitaci\u00f3n<\/td>344<\/td><\/tr>
Emisi\u00f3n<\/td>469<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

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Figura 1.<\/p>\n\n\n\n

Imagen de c\u00e9lulas HeLa te\u00f1idas con MitoLilte\u2122 Blue FX490 en una placa de fondo transparente de 96 pocillos.<\/p>\n\n\n\n

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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Cetyltrimethylammonium chloride-loaded mesoporous silica nanoparticles as a mitochondrion-targeting agent for tumor therapy<\/a>
Authors: <\/strong>Tang, Menghuan and Zhang, Peng and Liu, Jiahui and Long, Yijuan and Cheng, Yuan and Zheng, Huzhi
Journal: <\/strong>RSC Advances (2020): 17050–17057<\/p>\n\n\n\n

A ratiometric fluorescent probe for detecting hypochlorite in the endoplasmic reticulum<\/a>
Authors: <\/strong>Hou, Ji-Ting and Kim, Hyeong Seok and Duan, Chong and Ji, Myung Sun and Wang, Shan and Zeng, Lintao and Ren, Wen Xiu and Kim, Jong Seung
Journal: <\/strong>Chemical communications (2019): 2533–2536<\/p>\n\n\n\n

Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer<\/a>
Authors: <\/strong>Heger, Zbynek and Polanska, Hana and Krizkova, Sona and Balvan, Jan and Raudenska, Martina and Dostalova, Simona and Moulick, Amitava and Masarik, Michal and Adam, Vojtech
Journal: <\/strong>Colloids and Surfaces B: Biointerfaces (2017): 131–140<\/p>\n\n\n\n

Inhibition of heme oxygenase-1 enhances the chemosensitivity of laryngeal squamous cell cancer Hep-2 cells to cisplatin<\/a>
Authors: <\/strong>Lv, Xin and Song, Dong-mei and Niu, Ying-hao and Wang, Bao-shan
Journal: <\/strong>Apoptosis (2016): 489–501<\/p>\n\n\n\n

Effective two-photon excited photodynamic therapy of xenograft tumors sensitized by water-soluble bis (arylidene) cycloalkanone photosensitizers<\/a>
Authors: <\/strong>Zou, Qianli and Zhao, Hongyou and Zhao, Yuxia and Fang, Yanyan and Chen, Defu and Ren, Jie and Wang, Xiaopu and Wang, Ying and Gu, Ying and Wu, Feipeng
Journal: <\/strong>Journal of medicinal chemistry (2015): 7949–7958<\/p>\n\n\n\n

Melatonin promotes adipogenesis and mitochondrial biogenesis in 3T3-L1 preadipocytes<\/a>
Authors: <\/strong>Kato, Hisashi and Tanaka, Goki and Masuda, Shinya and Ogasawara, Junetsu and Sakurai, Takuya and Kizaki, Takako and Ohno, Hideki and Izawa, Tetsuya
Journal: <\/strong>Journal of Pineal Research (2015): 267–275<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 70 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Quantification of carbonylated proteins in rat skeletal muscle mitochondria using capillary sieving electrophoresis with laser-induced fluorescence detection
Authors: <\/strong>Feng J, Arriaga EA.
Journal: <\/strong>Electrophoresis (2008): 475<\/p>\n\n\n\n

Calcium, mitochondria and apoptosis studied by fluorescence measurements
Authors: <\/strong>Roy SS, Hajnoczky G.
Journal: <\/strong>Methods (2008): 213<\/p>\n\n\n\n

Fluorescence imaging of mitochondria in yeast
Authors: <\/strong>Swayne TC, Gay AC, Pon LA.
Journal: <\/strong>Methods Mol Biol (2007): 433<\/p>\n\n\n\n

A fluorescence assay for peptide translocation into mitochondria
Authors: <\/strong>Martinez-Caballero S, Peixoto PM, Kinnally KW, Campo ML.
Journal: <\/strong>Anal Biochem (2007): 76<\/p>\n\n\n\n

Fast electrophoretic analysis of individual mitochondria using microchip capillary electrophoresis with laser induced fluorescence detection
Authors: <\/strong>Duffy CF, MacCraith B, Diamond D, O’Kennedy R, Arriaga EA.
Journal: <\/strong>Lab Chip (2006): 1007<\/p>\n\n\n\n

Discrimination of depolarized from polarized mitochondria by confocal fluorescence resonance energy transfer
Authors: <\/strong>Elmore SP, Nishimura Y, Qian T, Herman B, Lemasters JJ.
Journal: <\/strong>Arch Biochem Biophys (2004): 145<\/p>\n\n\n\n

A fluorescence-based technique for screening compounds that protect against damage to brain mitochondria
Authors: <\/strong>Kristian T, Fiskum G.
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Determination of the cardiolipin content of individual mitochondria by capillary electrophoresis with laser-induced fluorescence detection
Authors: <\/strong>Fuller KM, Duffy CF, Arriaga EA.
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Fluorescence imaging of metabolic responses in single mitochondria
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Visualisation of mitochondria in living neurons with single- and two-photon fluorescence laser microscopy
Authors: <\/strong>Dedov VN, Cox GC, Roufogalis BD.
Journal: <\/strong>Micron (2001): 653<\/p>\n\n\n\n

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Application notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
Calcein<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Colorante cati\u00f3nico que se acumula selectivamente en las mitocondrias, probablemente a trav\u00e9s del gradiente de potencial de la membrana mitocondrial.<\/p>\n","protected":false},"featured_media":3980,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3978"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":4,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3978\/revisions"}],"predecessor-version":[{"id":3984,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3978\/revisions\/3984"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3980"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3978"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3978"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}