{"id":3913,"date":"2023-01-26T19:52:31","date_gmt":"2023-01-27T01:52:31","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3913"},"modified":"2023-01-26T21:10:17","modified_gmt":"2023-01-27T03:10:17","slug":"calbryte-520-potassium-salt","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/calbryte-520-potassium-salt\/","title":{"rendered":"Calbryte\u2122 520, potassium salt"},"content":{"rendered":"\n

La medici\u00f3n del calcio es fundamental para numerosas investigaciones biol\u00f3gicas. Las sondas fluorescentes que muestran respuestas espectrales al unirse al calcio han permitido a los cientificos investigar los cambios en las concentraciones de calcio libre celular mediante microscop\u00eda de fluorescencia, citometr\u00eda de flujo, espectroscop\u00eda de fluorescencia y lectores de microplacas de fluorescencia. <\/p>\n\n\n\n

Seguido por la introducci\u00f3n de Fluo-3 en 1989, Fluo-4, Fluo-8 y Cal-520 se desarrollaron m\u00e1s tarde con una mejor relaci\u00f3n se\u00f1al\/fondo. Sin embargo, todav\u00eda hay algunos problemas graves con Fluo-4. Por ejemplo, en cuanto a Fluo-3, en la mayor\u00eda de los ensayos de calcio intracelular con Fluo-4, se requiere probenecid para evitar que el Fluo-4 cargado en las c\u00e9lulas se escape de las c\u00e9lulas. El uso de probenecid con ensayos de calcio basados en Fluo-4 compromete los resultados del ensayo ya que est\u00e1 bien documentado que el probenecid tiene una variedad de efectos celulares complicados. <\/p>\n\n\n\n

Calbryte\u2122 520 es una nueva generaci\u00f3n de indicadores fluorescentes para mediciones de calcio, su relaci\u00f3n se\u00f1al\/ruido muy mejorada y mejores propiedades de retenci\u00f3n intracelular hacen de Calbryte\u2122 520 el indicador de calcio m\u00e1s s\u00f3lido. Tiene la misma longitud de onda de excitaci\u00f3n y emisi\u00f3n que Fluo-4, por lo que la misma configuraci\u00f3n de ensayo de Fluo-4 se puede aplicar f\u00e1cilmente a los ensayos de calcio basados en Calbryte\u2122 520.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-20656<\/td>Calbryte\u2122 520, potassium salt<\/td>2 x 50 ug<\/td><\/tr>
AAT-20658<\/td>Calbryte\u2122 520, potassium salt<\/td>10 x 50 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar a  (< -15 \u00b0C), Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>493<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>515<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.751<\/sup><\/td><\/tr><\/tbody><\/table>
1<\/sup> Calcium bound (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan
Volumen de agua necesario para reconstituir la masa espec\u00edfica de Calbryte\u2122 520, sal de potasio a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>110.009 \u00b5L<\/td>550.043 \u00b5L<\/td>1.1 mL<\/td>5.5 mL<\/td>11.001 mL<\/td><\/tr>
5 mM<\/strong><\/td>22.002 \u00b5L<\/td>110.009 \u00b5L<\/td>220.017 \u00b5L<\/td>1.1 mL<\/td>2.2 mL<\/td><\/tr>
10 mM<\/strong><\/td>11.001 \u00b5L<\/td>55.004 \u00b5L<\/td>110.009 \u00b5L<\/td>550.043 \u00b5L<\/td>1.1 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1.<\/strong> Espectros de emisi\u00f3n de fluorescencia de Calbryte\u2122 520 en una soluci\u00f3n que contiene de 0 a 39 \u00b5M de Ca2+ libre.<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Quantum yield<\/td><\/tr>
Calbryte\u2122 590, potassium salt<\/a><\/td>581<\/td>593<\/td>–<\/td><\/tr>
Calbryte\u2122 630, potassium salt<\/a><\/td>607<\/td>624<\/td>–<\/td><\/tr>
Calbryte\u2122-520L, potassium salt<\/a><\/td>493<\/td>515<\/td>0.751<\/sup><\/td><\/tr>
Calbryte\u2122-520XL, potassium salt<\/a><\/td>493<\/td>515<\/td>0.751<\/sup><\/td><\/tr>
Cal-520\u00ae, potassium salt<\/a><\/td>493<\/td>515<\/td>0.751<\/sup><\/td><\/tr>
Mag-520\u2122 potassium salt<\/a><\/td>507<\/td>531<\/td>–<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
Calbryte\u2122 520 AM<\/a><\/td><\/tr>
Calbryte\u2122 590 AM<\/a><\/td><\/tr>
Calbryte\u2122 630 AM<\/a><\/td><\/tr>
Calbryte\u2122-520L AM<\/a><\/td><\/tr>
Calbryte\u2122-520XL azide<\/a><\/td><\/tr>
Calbryte\u2122-520XL AM<\/a><\/td><\/tr>
Calbryte\u2122-520XL-Dextran<\/a><\/td><\/tr>
Chemical Phosphorylation Reagent I (CPR I)<\/a><\/td><\/tr>
Cell Meter\u2122 Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*<\/a><\/td><\/tr>
Cal Green\u2122 1, hexapotassium salt<\/a><\/td><\/tr>
Cal Green\u2122 1, AM [Equivalent to Calcium Green-1, AM]<\/a><\/td><\/tr>
Cal-590\u2122-Dextran Conjugate *MW 3,000*<\/a><\/td><\/tr>
Cal-590\u2122-Dextran Conjugate *MW 10,000*<\/a><\/td><\/tr>
Cal-590\u2122 AM<\/a><\/td><\/tr>
Cal-590\u2122, sodium salt<\/a><\/td><\/tr>
Cal-590\u2122, potassium salt<\/a><\/td><\/tr>
Cal-630\u2122 AM<\/a><\/td><\/tr>
Cal-630\u2122, sodium salt<\/a><\/td><\/tr>
Cal-630\u2122, potassium salt<\/a><\/td><\/tr>
Cal-630\u2122-Dextran Conjugate *MW 3,000*<\/a><\/td><\/tr>
Cal-630\u2122-Dextran Conjugate *MW 10,000*<\/a><\/td><\/tr>
Fluo-4 AM *Ultrapure Grade* *CAS 273221-67-3*<\/a><\/td><\/tr>
Fluo-4, Pentapotassium Salt<\/a><\/td><\/tr>
Cal Red\u2122 R525\/650 potassium salt<\/a><\/td><\/tr>
Cal Red\u2122 R525\/650 AM<\/a><\/td><\/tr>
Cal-520\u00ae-Dextran Conjugate *MW 3,000*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/mark><\/p>\n\n\n\n

Calreticulin regulates TGF-\u03b21-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling<\/a>
Authors: <\/strong>Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
Journal: <\/strong>The International Journal of Biochemistry & Cell Biology (2017)<\/p>\n\n\n\n

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells<\/a>
Authors: <\/strong>Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
Journal: <\/strong>Neural Regeneration Research (2017): 945<\/p>\n\n\n\n

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels<\/a>
Authors: <\/strong>Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
Journal: <\/strong>Scientific Reports (2017): 41781<\/p>\n\n\n\n

Dexmedetomidine reduces hypoxia\/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons<\/a>
Authors: <\/strong>Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
Journal: <\/strong>Int J Clin Exp Med (2017): 6861–6868<\/p>\n\n\n\n

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection<\/a>
Authors: <\/strong>Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
Journal: <\/strong>Journal of cellular and molecular medicine (2015): 581–594<\/p>\n\n\n\n

The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia\/reperfusion injury<\/a>
Authors: <\/strong>Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia
Journal: <\/strong>Biochemical and biophysical research communications (2015): 537–542<\/p>\n\n\n\n

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats<\/a>
Authors: <\/strong>Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min
Journal: <\/strong>Journal of Neural Transmission (2014): 593–600<\/p>\n\n\n\n

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem\/progenitor cells<\/a>
Authors: <\/strong>Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined
Journal: <\/strong>Neural regeneration research (2014): 2002<\/p>\n\n\n\n

Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.<\/a>
Authors: <\/strong>Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui
Journal: <\/strong>International journal of ophthalmology (2014): 441–447<\/p>\n\n\n\n

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis<\/a>
Authors: <\/strong>Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A
Journal: <\/strong>British Journal of Dermatology (2013): 771–778<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 53 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: <\/strong>Bailey S, Macardle PJ.
Journal: <\/strong>J Immunol Methods (2006): 220<\/p>\n\n\n\n

Functional fluo-3\/AM assay on P-glycoprotein transport activity in L1210\/VCR cells by confocal microscopy
Authors: <\/strong>Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: <\/strong>Gen Physiol Biophys (2004): 357<\/p>\n\n\n\n

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry
Authors: <\/strong>Patel H, Porter RH, Palmer AM, Croucher MJ.
Journal: <\/strong>Br J Pharmacol (2003): 671<\/p>\n\n\n\n

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics
Authors: <\/strong>Loughrey CM, MacEachern KE, Cooper J, Smith GL.
Journal: <\/strong>Cell Calcium (2003): 1<\/p>\n\n\n\n

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification
Authors: <\/strong>Zhang Z, Kitching P.
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Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang
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MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells
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Journal: <\/strong>Am J Physiol Gastrointest Liver Physiol (2000): G522<\/p>\n\n\n\n

Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: <\/strong>Walczysko P, Wagner E, Albrechtova JT.
Journal: <\/strong>Cell Calcium (2000): 23<\/p>\n\n\n\n

[Ca2+]i following extrasystoles in guinea-pig trabeculae microinjected with fluo-3 – a comparison with frog skeletal muscle fibres
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Journal: <\/strong>Acta Physiol Scand (2000): 1<\/p>\n\n\n\n

Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+) concentrations
Authors: <\/strong>Rockwell PL, Storey BT.
Journal: <\/strong>Mol Reprod Dev (1999): 418<\/p>\n\n\n\n

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Aplication Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells<\/a>
A Meta-Analysis of Common Calcium Indicators<\/a>
A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a>
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets<\/a>
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Calbryte\u2122 520 es una nueva generaci\u00f3n de indicadores fluorescentes para mediciones de calcio, su relaci\u00f3n se\u00f1al\/ruido muy mejorada y mejores propiedades de retenci\u00f3n intracelular hacen de Calbryte\u2122 520 el indicador de calcio m\u00e1s s\u00f3lido.<\/p>\n","protected":false},"featured_media":3915,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3913"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3913\/revisions"}],"predecessor-version":[{"id":3918,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3913\/revisions\/3918"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3915"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3913"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3913"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}