{"id":3907,"date":"2023-01-26T19:00:57","date_gmt":"2023-01-27T01:00:57","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3907"},"modified":"2023-01-27T12:43:09","modified_gmt":"2023-01-27T18:43:09","slug":"screen-quest-calbryte-590-probenecid-free-and-wash-free-calcium-assay-kit","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/screen-quest-calbryte-590-probenecid-free-and-wash-free-calcium-assay-kit\/","title":{"rendered":"Screen Quest\u2122 Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit"},"content":{"rendered":"\n

Los ensayos de flujo de calcio son los m\u00e9todos preferidos para la detecci\u00f3n de receptores acoplados a prote\u00edna G (GPCR) en el descubrimiento de f\u00e1rmacos. El kit de ensayo de calcio sin probenecid y sin lavado Screen Quest\u2122 Calbryte-590\u2122 proporciona el ensayo basado en fluorescencia roja homog\u00e9neo m\u00e1s s\u00f3lido para detectar la movilizaci\u00f3n de calcio intracelular. <\/p>\n\n\n\n

Las c\u00e9lulas que expresan un GPCR de inter\u00e9s que emite se\u00f1ales a trav\u00e9s del calcio est\u00e1n precargadas con nuestro Calbryte-590 AM patentado que puede atravesar la membrana celular. Calbryte-590 AM es el indicador de calcio m\u00e1s brillante disponible para la detecci\u00f3n de HTS. Una vez dentro de la c\u00e9lula, los grupos bloqueadores lipof\u00edlicos de Calbryte-590 AM son escindidos por esterasa celular no espec\u00edfica, lo que da como resultado un tinte fluorescente con carga negativa que permanece dentro de las c\u00e9lulas y su fluorescencia aumenta considerablemente al unirse al calcio. Cuando las c\u00e9lulas se estimulan con compuestos de detecci\u00f3n, el receptor se\u00f1ala la liberaci\u00f3n de calcio intracelular, lo que aumenta en gran medida la fluorescencia de Calbryte-590 AM. Las caracter\u00edsticas de su excelente retenci\u00f3n celular, alta sensibilidad y aumentos de fluorescencia de 100 a 250 veces (cuando forma complejos con calcio) hacen de Calbryte-590 AM un indicador ideal para la medici\u00f3n de calcio celular ya que es el \u00fanico tinte de calcio rojo que no requiere probenecid para mejorar la retenci\u00f3n celular. <\/p>\n\n\n\n

Este kit de ensayo de calcio Screen Quest\u2122 Calbryte-590\u2122 sin probenecid y sin lavado proporciona el m\u00e9todo de ensayo m\u00e1s optimizado para monitorear GPCR y canales de calcio con l\u00edneas celulares fr\u00e1giles o dif\u00edciles. En comparaci\u00f3n con los ensayos de calcio basados en fluorescencia verde, este kit de ensayo tiene menos interferencia de los compuestos coloreados de una biblioteca de compuestos. Tambi\u00e9n es compatible con l\u00edneas celulares GFP para an\u00e1lisis de alto contenido. <\/p>\n\n\n\n

El ensayo se puede realizar en un conveniente formato de placa de microtitulaci\u00f3n de 96 pocillos o de 384 pocillos y se puede adaptar f\u00e1cilmente a la automatizaci\u00f3n.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-36200<\/td>Screen Quest\u2122 Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit<\/td>1 placa<\/td><\/tr>
AAT-36201<\/td>Screen Quest\u2122 Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit<\/td>10 placas<\/td><\/tr>
AAT-36202<\/td>Screen Quest\u2122 Calbryte-590 Probenecid-Free and Wash-Free Calcium Assay Kit<\/td>100 placas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n
\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Fluorescencia<\/mark> <\/p>\n\n\n\n

Excitaci\u00f3n<\/td>540 nm<\/td><\/tr>
Emisi\u00f3n<\/td>590 nm<\/td><\/tr>
Cutoff<\/td>570 nm<\/td><\/tr>
Placa recomendada<\/td>Pared negra \/ fondo claro <\/td><\/tr>
Especificaciones instrumento<\/td>Modo de lectura inferior\/Manejo de l\u00edquidos programable<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Otros Instrumentos<\/mark><\/p>\n\n\n\n

ArrayScan, FDSS, FLIPR, FlexStation, IN Cell Analyzer, NOVOStar, ViewLux<\/p>\n\n\n\n

<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: Calbryte\u2122 590 AM<\/td>10 viales<\/td><\/tr>
Componente B: 10X Pluronic\u00ae F127 Plus<\/td>10 botellas (10 mL\/botella)<\/td><\/tr>
Componente C: HHBS (Hanks’ buffer con 20 mM Hepes)<\/td>No Incluido<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark> <\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>581<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>593<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n

PREPARACION DE SOLUCION DE STOCK<\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

1. Soluci\u00f3n madre Calbryte\u2122 590 AM: <\/em><\/p>\n\n\n\n

Agregue 20 \u00b5L (Cat. # 36200) o 200 \u00b5L (Cat. # 36201 y # 36202) de DMSO en el vial de Calbryte\u2122 590 AM (Componente A) y mezcle bien. Nota: 20 \u00b5L de soluci\u00f3n madre Calbryte\u2122 590 AM es suficiente para una placa. La soluci\u00f3n madre de Calbryte\u2122 590 AM sin usar puede dividirse en al\u00edcuotas y almacenarse a < -20 oC durante m\u00e1s de un mes si los tubos est\u00e1n sellados herm\u00e9ticamente. Nota: Proteger de la luz y evitar ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n. <\/p>\n\n\n\n

2. Buffer de ensayo (1X): <\/em><\/p>\n\n\n\n

Mezcle 9 mL de HHBS (Componente C, no incluido en el kit Cat. # 36202) con 1 mL de 10X Pluronic\u00ae F127 Plus (10X) (Componente B) y mezcle bien.<\/p>\n\n\n\n

\n\n\n\n

PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

Soluci\u00f3n de carga de tinte Calbryte\u2122 590 AM: <\/em><\/p>\n\n\n\n

Agregue 20 \u00b5L de soluci\u00f3n madre Calbryte\u2122 590 AM en 10 mL de Buffer de ensayo (1X) y mezcle bien. Nota: Esta soluci\u00f3n de trabajo es estable durante al menos 2 horas a temperatura ambiente. Nota: 10 ml de soluci\u00f3n de carga de colorante es suficiente para una placa de 96 pocillos.<\/p>\n\n\n\n

Para guia sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite:<\/p>\n\n\n\n

https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/p>\n<\/div><\/div>\n\n\n\n

<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1<\/strong>. La gr\u00e1fica ilustra la relaci\u00f3n se\u00f1al-ruido (SNR) x 100 %. La respuesta a la dosis de ATP se midi\u00f3 en c\u00e9lulas CHO-K1 con Screen Quest\u2122 Calbryte 590 Probenecid-Free and Wash-Free Calcium Assay Kit. Se sembraron c\u00e9lulas CHO-K1 durante la noche a 50.000 c\u00e9lulas\/100 \u00b5l\/pocillo en una placa costar de 96 pocillos de pared negra\/fondo transparente. Se a\u00f1adieron 100 \u00b5l de soluci\u00f3n de carga de colorante y se incubaron durante 45 min a 37 \u00b0C y 15 min a temperatura ambiente. FlexStation 3 a\u00f1adi\u00f3 ATP (50 \u00b5l\/pocillo) para alcanzar las concentraciones finales indicadas.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Relacionados<\/mark><\/p>\n\n\n\n

Screen Quest\u2122 Membrane Potential Assay Kit *Orange Fluorescence<\/a>*<\/a><\/td><\/tr>
Screen Quest\u2122 Membrane Potential Assay Kit *Red Fluorescence*<\/a><\/td><\/tr>
Screen Quest\u2122 10X cell staining buffer with Phenol Red Plus\u2122<\/a><\/td><\/tr>
Screen Quest\u2122 10X calcium assay buffer with Phenol Red Plus\u2122<\/a><\/td><\/tr>
Screen Quest\u2122 Luminometric Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluo-8 Medium Removal Calcium Assay Kit *Optimized for Difficult Cell Lines*<\/a><\/td><\/tr>
Screen Quest\u2122 Fluo-8 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fura-2 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Rhod-4 No Wash Calcium Assay Kit *Medium Removal*<\/a><\/td><\/tr>
Screen Quest\u2122 Rhod-4 No Wash Calcium Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric MDR Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Colorimetric Chloride Channel Assay Ki<\/a>t<\/a><\/td><\/tr>
Screen Quest\u2122 Colorimetric ELISA cAMP Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric ELISA cAMP Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 TR-FRET No Wash cAMP Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Live Cell cAMP Assay Service Pack<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric Fatty Acid Uptake Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Fluorimetric Glucose Uptake Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 Colorimetric Glucose Uptake Assay Kit<\/a><\/td><\/tr>
Screen Quest\u2122 HEK-CNGC-Amylin 3 Receptor Cells<\/a><\/td><\/tr>
Screen Quest\u2122 HEK-CNGC-Cannabinoid Receptor 1 Cells<\/a><\/td><\/tr>
Screen Quest\u2122 CHO-Ga16- Chemokine (C-C) Receptor 2B Cells<\/a><\/td><\/tr>
Screen Quest\u2122 HEK-CNGC-Chemokine (C-X-C motif) Receptor 4 Cells<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Calreticulin regulates TGF-\u03b21-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling<\/a>
Authors: <\/strong>Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling
Journal: <\/strong>The International Journal of Biochemistry & Cell Biology (2017)<\/p>\n\n\n\n

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells<\/a>
Authors: <\/strong>Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined
Journal: <\/strong>Neural Regeneration Research (2017): 945<\/p>\n\n\n\n

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels<\/a>
Authors: <\/strong>Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang
Journal: <\/strong>Scientific Reports (2017): 41781<\/p>\n\n\n\n

Dexmedetomidine reduces hypoxia\/reoxygenation injury by regulating mitochondrial fission in rat hippocampal neurons<\/a>
Authors: <\/strong>Liu, Jia and Du, Qing and Zhu, He and Li, Yu and Liu, Maodong and Yu, Shoushui and Wang, Shilei
Journal: <\/strong>Int J Clin Exp Med (2017): 6861–6868<\/p>\n\n\n\n

Di (2-ethylhexyl) phthalate-induced apoptosis in rat INS-1 cells is dependent on activation of endoplasmic reticulum stress and suppression of antioxidant protection<\/a>
Authors: <\/strong>Sun, Xia and Lin, Yi and Huang, Qiansheng and Shi, Junpeng and Qiu, Ling and Kang, Mei and Chen, Yajie and Fang, Chao and Ye, Ting and Dong, Sijun
Journal: <\/strong>Journal of cellular and molecular medicine (2015): 581–594<\/p>\n\n\n\n

The effect of mitochondrial calcium uniporter on mitochondrial fission in hippocampus cells ischemia\/reperfusion injury<\/a>
Authors: <\/strong>Zhao, Lantao and Li, Shuhong and Wang, Shilei and Yu, Ning and Liu, Jia
Journal: <\/strong>Biochemical and biophysical research communications (2015): 537–542<\/p>\n\n\n\n

Role of mitochondrial calcium uniporter in regulating mitochondrial fission in the cerebral cortexes of living rats<\/a>
Authors: <\/strong>Liang, Nan and Wang, Peng and Wang, Shilei and Li, Shuhong and Li, Yu and Wang, Jinying and Wang, Min
Journal: <\/strong>Journal of Neural Transmission (2014): 593–600<\/p>\n\n\n\n

Propofol and remifentanil at moderate and high concentrations affect proliferation and differentiation of neural stem\/progenitor cells<\/a>
Authors: <\/strong>Li, Qing and Lu, Jiang and Wang, Xianyu and others, undefined
Journal: <\/strong>Neural regeneration research (2014): 2002<\/p>\n\n\n\n

Fungus induces the release of IL-8 in human corneal epithelial cells, via Dectin-1-mediated protein kinase C pathways.<\/a>
Authors: <\/strong>Peng, Xu-Dong and Zhao, Gui-Qiu and Lin, Jing and Jiang, Nan and Xu, Qiang and Zhu, Cheng-Cheng and Qu, Jain-Qiu and Cong, Lin and Li, Hui
Journal: <\/strong>International journal of ophthalmology (2014): 441–447<\/p>\n\n\n\n

Increased expression of cell adhesion molecule 1 by mast cells as a cause of enhanced nerve–mast cell interaction in a hapten-induced mouse model of atopic dermatitis<\/a>
Authors: <\/strong>Hagiyama, M and Inoue, T and Furuno, T and Iino, T and Itami, S and Nakanishi, M and Asada, H and Hosokawa, Y and Ito, A
Journal: <\/strong>British Journal of Dermatology (2013): 771–778<\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las<\/em> 53 referencias: <\/em>Citation Explorer<\/a><\/span><\/p>\n\n\n\n

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux
Authors: <\/strong>Bailey S, Macardle PJ.
Journal: <\/strong>J Immunol Methods (2006): 220<\/p>\n\n\n\n

Functional fluo-3\/AM assay on P-glycoprotein transport activity in L1210\/VCR cells by confocal microscopy
Authors: <\/strong>Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.
Journal: <\/strong>Gen Physiol Biophys (2004): 357<\/p>\n\n\n\n

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry
Authors: <\/strong>Patel H, Porter RH, Palmer AM, Croucher MJ.
Journal: <\/strong>Br J Pharmacol (2003): 671<\/p>\n\n\n\n

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics
Authors: <\/strong>Loughrey CM, MacEachern KE, Cooper J, Smith GL.
Journal: <\/strong>Cell Calcium (2003): 1<\/p>\n\n\n\n

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification
Authors: <\/strong>Zhang Z, Kitching P.
Journal: <\/strong>J Virol Methods (2000): 187<\/p>\n\n\n\n

Kinetics of onset of mouse sperm acrosome reaction induced by solubilized zona pellucida: fluorimetric determination of loss of pH gradient between acrosomal lumen and medium monitored by dapoxyl (2-aminoethyl) sulfonamide and of intracellular Ca(2+) chang
Authors: <\/strong>Rockwell PL, Storey BT.
Journal: <\/strong>Mol Reprod Dev (2000): 335<\/p>\n\n\n\n

MRP2, a human conjugate export pump, is present and transports fluo 3 into apical vacuoles of Hep G2 cells
Authors: <\/strong>Cantz T, Nies AT, Brom M, Hofmann AF, Keppler D.
Journal: <\/strong>Am J Physiol Gastrointest Liver Physiol (2000): G522<\/p>\n\n\n\n

Use of co-loaded Fluo-3 and Fura Red fluorescent indicators for studying the cytosolic Ca(2+)concentrations distribution in living plant tissue
Authors: <\/strong>Walczysko P, Wagner E, Albrechtova JT.
Journal: <\/strong>Cell Calcium (2000): 23<\/p>\n\n\n\n

[Ca2+]i following extrasystoles in guinea-pig trabeculae microinjected with fluo-3 – a comparison with frog skeletal muscle fibres
Authors: <\/strong>Wohlfart B., undefined
Journal: <\/strong>Acta Physiol Scand (2000): 1<\/p>\n\n\n\n

Determination of the intracellular dissociation constant, K(D), of the fluo-3. Ca(2+) complex in mouse sperm for use in estimating intracellular Ca(2+) concentrations
Authors: <\/strong>Rockwell PL, Storey BT.
Journal: <\/strong>Mol Reprod Dev (1999): 418<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells<\/a>
A Meta-Analysis of Common Calcium Indicators<\/a>
A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a>
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets<\/a>
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Este kit de ensayo de calcio sin probenecid y sin lavado proporciona el m\u00e9todo de ensayo m\u00e1s optimizado para monitorear GPCR y canales de calcio con l\u00edneas celulares fr\u00e1giles o dif\u00edciles. <\/p>\n","protected":false},"featured_media":3910,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3907"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3907\/revisions"}],"predecessor-version":[{"id":3925,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3907\/revisions\/3925"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3910"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3907"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3907"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}