{"id":3312,"date":"2023-01-13T18:34:39","date_gmt":"2023-01-14T00:34:39","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3312"},"modified":"2023-01-20T12:46:30","modified_gmt":"2023-01-20T18:46:30","slug":"6-fam-phosphoramidite-5-fluorescein-phosphoramidite","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/6-fam-phosphoramidite-5-fluorescein-phosphoramidite\/","title":{"rendered":"6-FAM phosphoramidite [5′-Fluorescein phosphoramidite]"},"content":{"rendered":"\n

Hay varias formas de marcar un oligonucle\u00f3tido con fluoresce\u00edna. La elecci\u00f3n de la etiqueta se diversifica a\u00fan m\u00e1s, dependiendo de los requisitos espectrales. La fosforamidita de 5′-fluoresce\u00edna-CE, derivada del is\u00f3mero \u00fanico 6-carboxifluoresce\u00edna, la fosforamidita de 5′-hexaclorofluoresce\u00edna-CE (HEX) y la fosforamidita de 5′-tetraclorofluoresce\u00edna-CE (TET) se pueden usar para marcar eficientemente un oligonucle\u00f3tido en el 5 ‘-fin. El etiquetado del extremo 3′ de un oligo con fluoresce\u00edna se puede lograr utilizando CPG de 3’-fluoresce\u00edna. La escisi\u00f3n est\u00e1ndar y la desprotecci\u00f3n con hidr\u00f3xido de amonio liberan el oligo marcado con fluoresce\u00edna cuando se usa cualquiera de estos soportes.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-6016<\/td>6-FAM phosphoramidite [5′-Fluorescein phosphoramidite]<\/td>100 \u00b5moles<\/td><\/tr>
AAT-6017<\/td>6-FAM phosphoramidite [5′-Fluorescein phosphoramidite]<\/td>10×100 \u00b5moles<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar  (< -15 \u00b0C), Minimizar la exposici\u00f3n a la luz<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>843.94<\/td><\/tr>
Disolvente<\/td>MeCN<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan.<\/mark>
Volumen de MeCN necesario para reconstituir la masa espec\u00edfica de fosforamidita de 6-FAM [fosforamidita de 5′-fluoresce\u00edna] CAS 204697-37-0<\/em> a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>118.492 \u00b5L<\/td>592.459 \u00b5L<\/td>1.185 mL<\/td>5.925 mL<\/td>11.849 mL<\/td><\/tr>
5 mM<\/strong><\/td>23.698 \u00b5L<\/td>118.492 \u00b5L<\/td>236.984 \u00b5L<\/td>1.185 mL<\/td>2.37 mL<\/td><\/tr>
10 mM<\/strong><\/td>11.849 \u00b5L<\/td>59.246 \u00b5L<\/td>118.492 \u00b5L<\/td>592.459 \u00b5L<\/td>1.185 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de correcci\u00f3n (260 nm)<\/td>0.32<\/td><\/tr>
Factor de correcci\u00f3n (280 nm)<\/td>0.178<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>83000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>493<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>517<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

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Figura 1.<\/strong> Estructura quimica de 6-FAM phosphoramidite [5′-Fluorescein phosphoramidite] *CAS 204697-37-0*<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*<\/a><\/td>493<\/td>517<\/td>83000<\/td>0.32<\/td>0.178<\/td><\/tr>
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8*<\/a><\/td>493<\/td>517<\/td>83000<\/td>0.32<\/td>0.178<\/td><\/tr>
6-FAM Azide<\/a><\/td>493<\/td>517<\/td>83000<\/td>0.32<\/td>0.178<\/td><\/tr>
6-FAM Alkyne<\/a><\/td>493<\/td>517<\/td>83000<\/td>0.32<\/td>0.178<\/td><\/tr>
6-Fluorescein phosphoramidite<\/a><\/td>498<\/td>517<\/td>800001<\/sup><\/td>0.32<\/td>0.275<\/td><\/tr>
6-TET phosphoramidite [5′-Tetrachlorofluorescein phosphoramidite] *CAS#: 877049-90-6*<\/a><\/td>521<\/td>542<\/td>73000<\/td>–<\/td>0.13<\/td><\/tr>
6-HEX phosphoramidite [5′-Hexachlorofluorescein phosphoramidite]<\/a><\/td>533<\/td>559<\/td>73000<\/td>–<\/td>0.15<\/td><\/tr>
6-JOE Phosphoramidite<\/a><\/td>520<\/td>545<\/td>750001<\/sup><\/td>–<\/td>–<\/td><\/tr>
FAM-xtra\u2122 Phosphoramidite<\/a><\/td>493<\/td>517<\/td>83000<\/td>0.32<\/td>0.178<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Otros Productos Relacionados<\/mark><\/p>\n\n\n\n

5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5*<\/a><\/td><\/tr>
6-FAM [6-Carboxyfluorescein] *CAS 3301-79-9*<\/a><\/td><\/tr>
5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester] *CAS 117548-22-8*<\/a><\/td><\/tr>
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7*<\/a><\/td><\/tr>
5-SFX [5-FAM-X, SE] *CAS 148356-00-7*<\/a><\/td><\/tr>
5(6)-FAM ethylenediamine<\/a><\/td><\/tr>
5-FAM ethylenediamine<\/a><\/td><\/tr>
5(6)-FAM cadaverine<\/a><\/td><\/tr>
5-FAM cadaverine<\/a><\/td><\/tr>
5-FAM Azide<\/a><\/td><\/tr>
5-FAM Alkyne<\/a><\/td><\/tr>
FMOC-Asp(5\/6-FAM)-OH<\/a><\/td><\/tr>
FMOC-Asp(5-FAM)-OH<\/a><\/td><\/tr>
FMOC-Glu(5\/6-FAM)-OH<\/a><\/td><\/tr>
FMOC-Glu(5-FAM)-OH<\/a><\/td><\/tr>
FMOC-Lys(5\/6-FAM)-OH<\/a><\/td><\/tr>
FMOC-Lys(5-FAM)-OH *CAS 1242933-88-5*<\/a><\/td><\/tr>
5-FAM-YVAD-FMK<\/a><\/td><\/tr>
Fluorescein aldehyde [5-FAM aldehyde]<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Sequence-specific, self-reporting hairpin inversion probes
Authors: <\/strong>Browne KA., undefined
Journal: <\/strong>J Am Chem Soc (2005): 1989<\/p>\n\n\n\n

Modified oligonucleotides containing lithocholic acid in their backbones: their enhanced cellular uptake and their mimicking of hairpin structures
Authors: <\/strong>Kim SJ, Bang EK, Kwon HJ, Shim JS, Kim BH.
Journal: <\/strong>Chembiochem (2004): 1517<\/p>\n\n\n\n

Synthesis of HyBeacons and dual-labelled probes containing 2′-fluorescent groups for use in genetic analysis
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Reduced aggregation and improved specificity of G-rich oligodeoxyribonucleotides containing pyrazolo[3,4-d]pyrimidine guanine bases
Authors: <\/strong>Kutyavin IV, Lokhov SG, Afonina IA, Dempcy R, Gall AA, Gorn VV, Lukhtanov E, Metcalf M, Mills A, Reed MW, S and ers S, Shishkina I, Vermeulen NM.
Journal: <\/strong>Nucleic Acids Res (2002): 4952<\/p>\n\n\n\n

Diels–Alder bioconjugation of diene-modified oligonucleotides
Authors: <\/strong>Hill KW, Taunton-Rigby J, Carter JD, Kropp E, Vagle K, Pieken W, McGee DP, Husar GM, Leuck M, Anziano DJ, Sebesta DP.
Journal: <\/strong>J Org Chem (2001): 5352<\/p>\n\n\n\n

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles
Authors: <\/strong>Ramirez-Vick JE, Garcia AA, Lee J.
Journal: <\/strong>Prep Biochem Biotechnol (1998): 243<\/p>\n\n\n\n

Simultaneous detection of several oligonucleotides by time-resolved fluorometry: the use of a mixture of categorized microparticles in a sandwich type mixed-phase hybridization assay
Authors: <\/strong>Hakala H, Virta P, Salo H, Lonnberg H.
Journal: <\/strong>Nucleic Acids Res (1998): 5581<\/p>\n\n\n\n

Synthesis and characterization of a new 5-thiol-protected deoxyuridine phosphoramidite for site-specific modification of DNA
Authors: <\/strong>Meyer KL, Hanna MM.
Journal: <\/strong>Bioconjug Chem (1996): 401<\/p>\n\n\n\n

Synthesis and characterisation of fluorescent oligonucleotides. Effect of internal labelling on protein recognition
Authors: <\/strong>Hagmar P, Bailey M, Tong G, Haralambidis J, Sawyer WH, Davidson BE.
Journal: <\/strong>Biochim Biophys Acta (1995): 259<\/p>\n\n\n\n

Characterization of endonucleolytic activity of HIV-1 integrase using a fluorogenic substrate
Authors: <\/strong>Lee SP, Censullo ML, Kim HG, Knutson JR, Han MK.
Journal: <\/strong>Anal Biochem (1995): 295<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
<\/a>Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
<\/a>Annexin V<\/a>
<\/a>Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
<\/a>Calcein<\/a><\/p>\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Fosforamidita de 6-FAM [fosforamidita de 5′-fluoresce\u00edna] *CAS 204697-37-0*<\/p>\n","protected":false},"featured_media":3316,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3312"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":5,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3312\/revisions"}],"predecessor-version":[{"id":3707,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3312\/revisions\/3707"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3316"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3312"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3312"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}