{"id":3308,"date":"2023-01-13T17:46:27","date_gmt":"2023-01-13T23:46:27","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3308"},"modified":"2023-04-17T11:45:11","modified_gmt":"2023-04-17T16:45:11","slug":"6-rox-se-6-carboxy-x-rhodamine-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/6-rox-se-6-carboxy-x-rhodamine-succinimidyl-ester\/","title":{"rendered":"6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]"},"content":{"rendered":"\n

Los is\u00f3meros \u00fanicos de ROX se prefieren cada vez m\u00e1s para marcar p\u00e9ptidos y nucle\u00f3tidos porque brindan una mejor resoluci\u00f3n en las purificaciones de HPLC que a menudo se requieren en las bioconjugaciones. <\/p>\n\n\n\n

6-ROX, SE es el otro is\u00f3mero \u00fanico purificado de 5(6)-ROX, SE. 6-ROX se usa predominantemente para marcar nucle\u00f3tidos y secuenciar \u00e1cidos nucleicos. (Ver cat. AAT-389 para 5-ROX SE)<\/p>\n\n\n\n

En comparaci\u00f3n con otras rodaminas, ROX es muy inestable. Se deben tomar precauciones para evitar el deterioro durante el almacenamiento.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-392<\/td>6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]<\/td>5 mg<\/td><\/tr>
AAT-399<\/td>6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester]<\/td>100 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar  (< -15 \u00b0C), Minimizar la exposici\u00f3n a la luz<\/p>\n\n\n\n

<\/div>\n\n\n\n

Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>631.67<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan.
Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de  6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4* a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>158.311 \u00b5L<\/td>791.553 \u00b5L<\/td>1.583 mL<\/td>7.916 mL<\/td>15.831 mL<\/td><\/tr>
5 mM<\/strong><\/td>31.662 \u00b5L<\/td>158.311 \u00b5L<\/td>316.621 \u00b5L<\/td>1.583 mL<\/td>3.166 mL<\/td><\/tr>
10 mM<\/strong><\/td>15.831 \u00b5L<\/td>79.155 \u00b5L<\/td>158.311 \u00b5L<\/td>791.553 \u00b5L<\/td>1.583 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de correci\u00f3n (280 nm)<\/td>0.168<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>82000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>578<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>604<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.941<\/sup><\/td><\/tr><\/tbody><\/table>
1 Buffer acuoso (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"<\/figure>\n\n\n\n

Figura 1.<\/strong> Estructura quimica de 6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS 216699-36-4*<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Relacionados<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Correction Factor (280 nm)<\/td><\/tr>
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*<\/a><\/td>522<\/td>546<\/td>94000<\/td>0.214<\/td><\/tr>
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8*<\/a><\/td>552<\/td>578<\/td>90000<\/td>0.178<\/td><\/tr>
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.168<\/td><\/tr>
5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7*<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.168<\/td><\/tr>
6-FAM, SE [6-Carboxyfluorescein, succinimidyl ester] *CAS 92557-81-8*<\/a><\/td>493<\/td>517<\/td>83000<\/td>0.178<\/td><\/tr>
6-HEX, SE [6-Carboxy-2′,4,4′,5′,7,7′-hexachlorofluorescein, succinimidyl ester] *Single Isomer*<\/a><\/td>533<\/td>559<\/td>73000<\/td>0.15<\/td><\/tr>
6-JOE, SE [6-Carboxy-4′,5′-dichloro-2′,7′-dimethoxyfluorescein, succinimidyl ester] *CAS#: 113394-23-3*<\/a><\/td>520<\/td>545<\/td>750001<\/sup><\/td>–<\/td><\/tr>
6-TET, SE [6-Carboxy-2′,4,7′,7-tetrachlorofluorescein, succinimidyl ester]<\/a><\/td>521<\/td>542<\/td>73000<\/td>0.13<\/td><\/tr>
6-ROX glycine *25 uM fluorescence reference solution for PCR reactions*<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.168<\/td><\/tr>
6-ROX azide<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.168<\/td><\/tr>
6-ROX alkyne<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.168<\/td><\/tr>
6-ROXtra\u2122 SE *Superior 6-ROX Replacement*<\/a><\/td>578<\/td>595<\/td>–<\/td>–<\/td><\/tr>
6-VIC, SE [6-VIC NHS ester]<\/a><\/td>526<\/td>543<\/td>–<\/td>–<\/td><\/tr>
6-NED, SE [6-NED NHS ester]<\/a><\/td>545<\/td>567<\/td>740002<\/sup><\/td><\/tr><\/tbody><\/table>
1 <\/sup>Buffer pH9, 2<\/sup> PBS<\/figcaption><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Otros Productos Relacionados<\/mark><\/p>\n\n\n\n

Rhodamine 700 *CAS 63561-42-2*<\/a><\/td><\/tr>
Rhodamine 800 *CAS 137993-41-0*<\/a><\/td><\/tr>
Rhodamine B *CAS 81-88-9*<\/a><\/td><\/tr>
Rhodamine 6G *CAS 989-38-8*<\/a><\/td><\/tr>
Sulforhodamine 101, sodium salt<\/a><\/td><\/tr>
Sulforhodamine B *CAS 3520-42-1*<\/a><\/td><\/tr>
Sulforhodamine G *CAS 5873-16-5*<\/a><\/td><\/tr>
Rhodamine 101 Inner Salt *CAS 116450-56-7*<\/a><\/td><\/tr>
Rhodamine 101 Chloride Salt *CAS 64339-18-0*<\/a><\/td><\/tr>
Sulforhodamine 101 sodium salt *CAS 465544-24-5*<\/a><\/td><\/tr>
Rhodamine 110 *CAS 13558-31-1*<\/a><\/td><\/tr>
5-CR110 [5-Carboxyrhodamine 110] *Single isomer*<\/a><\/td><\/tr>
6-CR110 [6-Carboxyrhodamine 110] *Single isomer*<\/a><\/td><\/tr>
5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]<\/a><\/td><\/tr>
5-CR6G [5-Carboxyrhodamine 6G] *Single isomer*<\/a><\/td><\/tr>
6-CR6G [6-Carboxyrhodamine 6G] *Single isomer*<\/a><\/td><\/tr>
5(6)-CR6G, SE [5-(and 6)-Carboxyrhodamine 6G, succinimidyl ester] *Mixed isomers*<\/a><\/td><\/tr>
6-CR6G, SE [6-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*<\/a><\/td><\/tr>
5(6)-Carboxyrhodamine 6G cadaverine<\/a><\/td><\/tr>
5(6)-Carboxyrhodamine 6G ethylenediamine<\/a><\/td><\/tr>
5(6)-TAMRA [5(6)-Carboxytetramethylrhodamine] *CAS 98181-63-6*<\/a><\/td><\/tr>
5-TAMRA [5-Carboxytetramethylrhodamine] *CAS 91809-66-4*<\/a><\/td><\/tr>
6-TAMRA [6-Carboxytetramethylrhodamine] *CAS 91809-67-5*<\/a><\/td><\/tr>
5(6)-TAMRA, SE [5-(and-6)-Carboxytetramethylrhodamine, succinimidyl ester] *CAS 246256-50-8*<\/a><\/td><\/tr>
6-TAMRA, SE [6-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-69-8*<\/a><\/td><\/tr>
5-ROX [5-Carboxy-X-rhodamine] *CAS 216699-35-3*<\/a><\/td><\/tr>
6-ROX [6-Carboxy-X-rhodamine] *CAS 194785-18-7*<\/a><\/td><\/tr>
5(6)-ROX, SE [5-(and-6)-Carboxy-X-rhodamine, succinimidyl ester] *Mixed isomers*<\/a><\/td><\/tr>
5(6)-TRITC [Tetramethylrhodamine-5-(and-6)-isothiocyanate] *CAS 95197-95-8*<\/a><\/td><\/tr>
5(6)-TAMRA Maleimide [Tetramethylrhodamine-5-(and-6)-maleimide] *Mixed isomers*<\/a><\/td><\/tr>
5-TRITC [Tetramethylrhodamine-5-isothiocyanate] *CAS 80724-19-2*<\/a><\/td><\/tr>
6-TRITC [Tetramethylrhodamine-6-isothiocyanate] *CAS 80724-20-5*<\/a><\/td><\/tr>
6-TAMRA Maleimide [Tetramethylrhodamine-6-maleimide] *CAS 174568-68-4*<\/a><\/td><\/tr>
5-TAMRA Maleimide [Tetramethylrhodamine-5-maleimide] *CAS 154480-30-5*<\/a><\/td><\/tr>
Lissamine Rhodamine B Sulfonyl Chloride [Sulforhodamine B sulfonyl chloride] *CAS 62796-29-6*<\/a><\/td><\/tr>
Sulforhodamine 101 sulfonyl chloride [Texas Red\u00ae] *CAS 82354-19-6*<\/a><\/td><\/tr>
SRB-VAD-FMK [Sulforhodamine B-VAD-FMK]<\/a><\/td><\/tr>
Dihydrorhodamine 123 *CAS 109244-58-8*<\/a><\/td><\/tr>
Tetramethylrhodamine-dUTP *1 mM in Tris Buffer (pH 7.5)*<\/a><\/td><\/tr>
Rhodamine 123 *CAS 62669-70-9*<\/a><\/td><\/tr>
Rhodamine B, hexyl ester, perchlorate<\/a><\/td><\/tr>
TMRE [Tetramethylrhodamine ethyl ester] *CAS#: 115532-52-0*<\/a><\/td><\/tr>
TMRM [Tetramethylrhodamine methyl ester] *CAS#: 115532-50-8*<\/a><\/td><\/tr>
Phalloidin-Tetramethylrhodamine Conjugate<\/a><\/td><\/tr>
5-dR6G [5-Carboxy-4,7-dichlororhodamine 6G]<\/a><\/td><\/tr>
6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G]<\/a><\/td><\/tr>
5-dR110 [5-Carboxy-4,7-dichlororhodamine 110]<\/a><\/td><\/tr>
6-dR110 [6-Carboxy-4,7-dichlororhodamine 110]<\/a><\/td><\/tr>
Bis-L-Aspartic acid Beta-rhodamine 110<\/a><\/td><\/tr>
Bis-Arg-rhodamine 110<\/a><\/td><\/tr>
Sulforhodamine 101 *CAS 60311-02-6*<\/a><\/td><\/tr>
5-Carboxyrhodamine 6G maleimide<\/a><\/td><\/tr>
Rhodamine aldehyde [5-TAMRA aldehyde]<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: <\/strong>Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: <\/strong>Proc Natl Acad Sci U S A (2005): 5926<\/p>\n\n\n\n

Adsorption of oligonucleotides on PMMA\/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: <\/strong>Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: <\/strong>Langmuir (2004): 6834<\/p>\n\n\n\n

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: <\/strong>Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: <\/strong>Proc Natl Acad Sci U S A (2004): 5488<\/p>\n\n\n\n

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: <\/strong>Faulds K, Smith WE, Graham D.
Journal: <\/strong>Anal Chem (2004): 412<\/p>\n\n\n\n

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
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Journal: <\/strong>Invest Ophthalmol Vis Sci (2003): 1312<\/p>\n\n\n\n

HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: <\/strong>Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: <\/strong>Tissue Antigens (2001): 250<\/p>\n\n\n\n

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction–amplified short tandem repeats during denaturing capillary electrophoresis
Authors: <\/strong>Hahn M, Wilhelm J, Pingoud A.
Journal: <\/strong>Electrophoresis (2001): 2691<\/p>\n\n\n\n

Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: <\/strong>Li Y, Glazer AN.
Journal: <\/strong>Bioconjug Chem (1999): 241<\/p>\n\n\n\n

Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: <\/strong>Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: <\/strong>Anal Biochem (1998): 82<\/p>\n\n\n\n

Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: <\/strong>Hung SC, Mathies RA, Glazer AN.
Journal: <\/strong>Anal Biochem (1998): 32<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes <\/mark><\/p>\n\n\n\n

Sunnyvale Red\u2122 SE, a Superior Replacement for 6-ROX SE<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

6-ROX se usa predominantemente para marcar nucle\u00f3tidos y secuenciar \u00e1cidos nucleicos. *CAS#: 216699-36-4*<\/p>\n","protected":false},"featured_media":3309,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3308"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":4,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3308\/revisions"}],"predecessor-version":[{"id":10993,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3308\/revisions\/10993"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3309"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3308"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3308"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}