{"id":3299,"date":"2023-01-13T14:00:30","date_gmt":"2023-01-13T20:00:30","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3299"},"modified":"2023-01-20T12:53:09","modified_gmt":"2023-01-20T18:53:09","slug":"5-rox-se-5-carboxy-x-rhodamine-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/5-rox-se-5-carboxy-x-rhodamine-succinimidyl-ester\/","title":{"rendered":"5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester]"},"content":{"rendered":"\n

Los is\u00f3meros \u00fanicos de ROX se prefieren cada vez m\u00e1s para marcar p\u00e9ptidos y nucle\u00f3tidos porque brindan una mejor resoluci\u00f3n en las purificaciones de HPLC que a menudo se requieren en las bioconjugaciones. Las proporciones variables de los is\u00f3meros 5 y 6 a menudo causan complicaciones en la interpretaci\u00f3n de los resultados del etiquetado y el rendimiento del ensayo. 5-ROX se usa predominantemente para marcar p\u00e9ptidos y prote\u00ednas. En comparaci\u00f3n con otras rodaminas, ROX es muy inestable. Se deben tomar precauciones para evitar el deterioro durante el almacenamiento.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-389<\/td>5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester]<\/td>5 mg<\/td><\/tr>
AAT-391<\/td>5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester]<\/td>100 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar  (< -15 \u00b0C), Minimizar la exposici\u00f3n a la luz<\/p>\n\n\n\n

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Propiedades fisicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>631.67<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan.<\/mark>
Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de 5-ROX, SE [5-carboxi-X-rodamina, succinimidil \u00e9ster] CAS 209734-74-7<\/em> a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>158.311 \u00b5L<\/td>791.553 \u00b5L<\/td>1.583 mL<\/td>7.916 mL<\/td>15.831 mL<\/td><\/tr>
5 mM<\/strong><\/td>31.662 \u00b5L<\/td>158.311 \u00b5L<\/td>316.621 \u00b5L<\/td>1.583 mL<\/td>3.166 mL<\/td><\/tr>
10 mM<\/strong><\/td>15.831 \u00b5L<\/td>79.155 \u00b5L<\/td>158.311 \u00b5L<\/td>791.553 \u00b5L<\/td>1.583 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de correci\u00f3n (280 nm)<\/td>0.168<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>82000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>578<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>604<\/td><\/tr>
Rendimiento cu\u00e1ntico<\/td>0.941<\/sup><\/td><\/tr><\/tbody><\/table>
1 Buffer acuoso (pH 7.2)<\/figcaption><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

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Figura 1.<\/strong> Estructura quimica de 5-ROX, SE [5-Carboxy-X-rhodamine, succinimidyl ester] *CAS 209734-74-7*<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Name<\/td>Excitation (nm)<\/td>Emission (nm)<\/td>Extinction coefficient (cm -1<\/sup> M -1<\/sup>)<\/td>Quantum yield<\/td>Correction Factor (260 nm)<\/td>Correction Factor (280 nm)<\/td><\/tr>
5-CR6G, SE [5-Carboxyrhodamine 6G, succinimidyl ester] *Single isomer*<\/a><\/td>522<\/td>546<\/td>94000<\/td>–<\/td>0.24<\/td>0.214<\/td><\/tr>
5-TAMRA, SE [5-Carboxytetramethylrhodamine, succinimidyl ester] *CAS#: 150810-68-7*<\/a><\/td>552<\/td>578<\/td>90000<\/td>–<\/td>0.32<\/td>0.178<\/td><\/tr>
5(6)-ROX [5-(and 6)-Carboxy-X-rhodamine] *CAS 198978-94-8*<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.941<\/sup><\/td>–<\/td>0.168<\/td><\/tr>
6-ROX, SE [6-Carboxy-X-rhodamine, succinimidyl ester] *CAS#: 216699-36-4*<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.941<\/sup><\/td>–<\/td>0.168<\/td><\/tr>
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7*<\/a><\/td>493<\/td>517<\/td>83000<\/td>–<\/td>0.32<\/td>0.178<\/td><\/tr>
5-ROX glycine *Fluorescence reference standard for PCR reactions*<\/a><\/td>578<\/td>604<\/td>82000<\/td>0.941<\/sup><\/td>–<\/td>0.168<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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5-CR110 [5-Carboxyrhodamine 110] *Single isomer*<\/a><\/td><\/tr>
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5(6)-CR6G [5-(and 6)-Carboxyrhodamine 6G]<\/a><\/td><\/tr>
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6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G]<\/a><\/td><\/tr>
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<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Four-color DNA sequencing by synthesis on a chip using photocleavable fluorescent nucleotides
Authors: <\/strong>Seo TS, Bai X, Kim DH, Meng Q, Shi S, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: <\/strong>Proc Natl Acad Sci U S A (2005): 5926<\/p>\n\n\n\n

Adsorption of oligonucleotides on PMMA\/PNIPAM core-shell latexes: polarity of the PNIPAM shell probed by fluorescence
Authors: <\/strong>Prazeres TJ, Santos AM, Martinho JM, Elaissari A, Pichot C.
Journal: <\/strong>Langmuir (2004): 6834<\/p>\n\n\n\n

Photocleavable fluorescent nucleotides for DNA sequencing on a chip constructed by site-specific coupling chemistry
Authors: <\/strong>Seo TS, Bai X, Ruparel H, Li Z, Turro NJ, Ju J.
Journal: <\/strong>Proc Natl Acad Sci U S A (2004): 5488<\/p>\n\n\n\n

Evaluation of surface-enhanced resonance Raman scattering for quantitative DNA analysis
Authors: <\/strong>Faulds K, Smith WE, Graham D.
Journal: <\/strong>Anal Chem (2004): 412<\/p>\n\n\n\n

Blue light-induced generation of reactive oxygen species in photoreceptor ellipsoids requires mitochondrial electron transport
Authors: <\/strong>Yang JH, Basinger SF, Gross RL, Wu SM.
Journal: <\/strong>Invest Ophthalmol Vis Sci (2003): 1312<\/p>\n\n\n\n

HLA-DRB fluorotyping by dark quenching and automated analysis
Authors: <\/strong>Slateva K, Elsner HA, Albis-Camps M, Blasczyk R.
Journal: <\/strong>Tissue Antigens (2001): 250<\/p>\n\n\n\n

Influence of fluorophor dye labels on the migration behavior of polymerase chain reaction–amplified short tandem repeats during denaturing capillary electrophoresis
Authors: <\/strong>Hahn M, Wilhelm J, Pingoud A.
Journal: <\/strong>Electrophoresis (2001): 2691<\/p>\n\n\n\n

Design, synthesis, and spectroscopic properties of peptide-bridged fluorescence energy-transfer cassettes
Authors: <\/strong>Li Y, Glazer AN.
Journal: <\/strong>Bioconjug Chem (1999): 241<\/p>\n\n\n\n

Differential display with carboxy-X-rhodamine-labeled primers and the selection of differentially amplified cDNA fragments without cloning
Authors: <\/strong>Yoshikawa Y, Mukai H, Asada K, Hino F, Kato I.
Journal: <\/strong>Anal Biochem (1998): 82<\/p>\n\n\n\n

Comparison of fluorescence energy transfer primers with different donor-acceptor dye combinations
Authors: <\/strong>Hung SC, Mathies RA, Glazer AN.
Journal: <\/strong>Anal Biochem (1998): 32<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

*CAS 209734-74-7*<\/p>\n","protected":false},"featured_media":3304,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3299"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":5,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3299\/revisions"}],"predecessor-version":[{"id":3711,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3299\/revisions\/3711"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3304"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3299"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3299"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}