{"id":3280,"date":"2023-01-13T12:26:37","date_gmt":"2023-01-13T18:26:37","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3280"},"modified":"2023-03-10T18:51:38","modified_gmt":"2023-03-11T00:51:38","slug":"tide-fluor-5ws-succinimidyl-ester-tf5ws-se","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/tide-fluor-5ws-succinimidyl-ester-tf5ws-se\/","title":{"rendered":"Tide Fluor\u2122 5WS succinimidyl ester [TF5WS SE]"},"content":{"rendered":"\n

La familia Tide Fluor\u2122 5WS (TF5WS) tiene propiedades espectrales esencialmente id\u00e9nticas a las de Cy5. En comparaci\u00f3n con las sondas Cy5, la familia TF5WS tiene una fluorescencia mucho m\u00e1s fuerte y una mayor fotoestabilidad. Adem\u00e1s, su fluorescencia es independiente del pH entre pH 3 y 11. Estas caracter\u00edsticas hacen de esta nueva familia de colorantes una alternativa superior a Cy5. Los p\u00e9ptidos y nucle\u00f3tidos marcados con TF5WS exhiben una fluorescencia mucho m\u00e1s fuerte y una fotoestabilidad m\u00e1s alta que los marcados con Cy5. En combinaci\u00f3n con nuestro Tide Quencher\u2122 5 (TQ5), se puede desarrollar una variedad de p\u00e9ptidos y nucle\u00f3tidos FRET para detectar proteasas y balizas moleculares con mayor sensibilidad y estabilidad.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-2281<\/td>Tide Fluor\u2122 5WS succinimidyl ester [TF5WS SE]<\/td>5 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar  (< -15 \u00b0C), Minimizar la exposici\u00f3n a la luz<\/p>\n\n\n\n

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Propiedades f\u00edsicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>1050.35<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Factor de correci\u00f3n (280 nm)<\/td>0.027<\/td><\/tr>
Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>250000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>649<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>664<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark>
Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de Tide Fluor\u2122 5WS succinimidil \u00e9ster [TF5WS SE] (Reemplazo superior para Cy5<\/em>) a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffer experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>95.206 \u00b5L<\/td>476.032 \u00b5L<\/td>952.064 \u00b5L<\/td>4.76 mL<\/td>9.521 mL<\/td><\/tr>
5 mM<\/strong><\/td>19.041 \u00b5L<\/td>95.206 \u00b5L<\/td>190.413 \u00b5L<\/td>952.064 \u00b5L<\/td>1.904 mL<\/td><\/tr>
10 mM<\/strong><\/td>9.521 \u00b5L<\/td>47.603 \u00b5L<\/td>95.206 \u00b5L<\/td>476.032 \u00b5L<\/td>952.064 \u00b5L<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/mark><\/p>\n\n\n\n

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Figura 1.<\/strong> Los \u00e9steres de NHS de tinte fluorescente (o \u00e9steres de succinimidilo) son la herramienta m\u00e1s popular para conjugar tintes con un p\u00e9ptido, prote\u00edna, anticuerpo, oligonucle\u00f3tido modificado con amino o \u00e1cido nucleico. Los \u00e9steres de NHS reaccionan r\u00e1pidamente con las aminas primarias (R-NH2) de prote\u00ednas, oligonucle\u00f3tidos modificados con amina y otras mol\u00e9culas que contienen amina. Los conjugados de tinte resultantes son bastante estables.<\/p>\n\n\n\n

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Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Inhibiting fibronectin polymerization alleviates kidney injury due to ischemia\/reperfusion<\/a>
Authors: <\/strong>Bowers, Stephanie LK and Davis-Rodriguez, Stephanie and Thomas, Zachary M and Rudomanova, Valeriia and Bacon, W Clark and Beiersdorfer, Alex and Ma, Qing and Devarajan, Prasad and Blaxall, Burns C
Journal: <\/strong>American Journal of Physiology-Renal Physiology (2019)<\/p>\n\n\n\n

A mechanistic model to predict effects of cathepsin B and cystatin C on \u03b2-amyloid aggregation and degradation<\/a>
Authors: <\/strong>Perlenfein, Tyler J and Murphy, Regina M
Journal: <\/strong>Journal of Biological Chemistry (2017): jbc–M117<\/p>\n\n\n\n

Real-Time Detection of a Self-Replicating RNA Enzyme<\/a>
Authors: <\/strong>Olea, Charles and Joyce, Gerald F
Journal: <\/strong>Molecules (2016): 1310<\/p>\n\n\n\n

Maternal serum glycosylated fibronectin as a point-of-care biomarker for assessment of preeclampsia<\/a>
Authors: <\/strong>Rasanen, Juha and Quinn, Matthew J and Laurie, Amber and Bean, Eric and Roberts, Charles T and Nagalla, Srinivasa R and Gravett, Michael G
Journal: <\/strong>American journal of obstetrics and gynecology (2015): 82–e1<\/p>\n\n\n\n

Development of Multi-Parametric\/Multimodal Spectroscopy Apparatus for Characterization of Functional Interfaces<\/a>
Authors: <\/strong>Zhou, Lang and Arugula, Mary and Easley, Christopher J and Shannon, Curtis and Simonian, Aleks and r, undefined
Journal: <\/strong>ECS Transactions (2015): 9–16<\/p>\n\n\n\n

Development of a universal RNA beacon for exogenous gene detection<\/a>
Authors: <\/strong>Guo, Yuanjian and Lu, Zhongju and Cohen, Ira Stephen and Scarlata, Suzanne
Journal: <\/strong>Stem cells translational medicine (2015): 476–482<\/p>\n\n\n\n

Bio-reduction of Disulfide-Containing Drug Carriers<\/a>
Authors: <\/strong>Br{\\”u}lisauer, Lorine
Journal: <\/strong>(2014)<\/p>\n\n\n\n

Array of biodegradable microrafts for isolation and implantation of living, adherent cells<\/a>
Authors: <\/strong>Wang, Yuli and Phillips, Colleen N and Herrera, Gabriela S and Sims, Christopher E and Yeh, Jen Jen and Allbritton, Nancy L
Journal: <\/strong>RSC advances (2013): 9264–9272<\/p>\n\n\n\n

Development of SNAP-Tag Fluorogenic Probes for Wash-Free Fluorescence Imaging<\/a>
Authors: <\/strong>Sun, Xiaoli and Zhang, Aihua and Baker, Brenda and Sun, Luo and Howard, Angela and Buswell, John and Maurel, Damien and Masharina, Anastasiya and Johnsson, Kai and Noren, Christopher J and others, undefined
Journal: <\/strong>ChemBioChem (2011): 2217–2226<\/p>\n\n\n\n

FERRAMENTAS PARA ESTUDO DA BIOLOGIA DE GPCRS (G-PROTEIN COUPLED RECEPTORS)<\/a>
Authors: <\/strong>Soriani, Frederico Marianetti and Russo, Remo Castro<\/p>\n\n\n\n

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Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 25 referencias: <\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Time-resolved FRET method for typing polymorphic alleles of the human leukocyte antigen system by using a single DNA probe
Authors: <\/strong>Andreoni A, Bondani M, Nardo L.
Journal: <\/strong>Photochem Photobiol Sci (2009): 1202<\/p>\n\n\n\n

Tumor-specific detection of an optically targeted antibody combined with a quencher-conjugated neutravidin “quencher-chaser”: a dual “quench and chase” strategy to improve target to nontarget ratios for molecular imaging of cancer
Authors: <\/strong>Ogawa M, Kosaka N, Choyke PL, Kobayashi H.
Journal: <\/strong>Bioconjug Chem (2009): 147<\/p>\n\n\n\n

The detection of platelet derived growth factor using decoupling of quencher-oligonucleotide from aptamer\/quantum dot bioconjugates
Authors: <\/strong>Kim GI, Kim KW, Oh MK, Sung YM.
Journal: <\/strong>Nanotechnology (2009): 175503<\/p>\n\n\n\n

Development of a cell-based hepatitis C virus infection fluorescent resonance energy transfer assay for high-throughput antiviral compound screening
Authors: <\/strong>Yu X, Sainz B, Jr., Uprichard SL.
Journal: <\/strong>Antimicrob Agents Chemother (2009): 4311<\/p>\n\n\n\n

An improved cell-penetrating, caspase-activatable, near-infrared fluorescent peptide for apoptosis imaging
Authors: <\/strong>Maxwell D, Chang Q, Zhang X, Barnett EM, Piwnica-Worms D.
Journal: <\/strong>Bioconjug Chem (2009): 702<\/p>\n\n\n\n

Feasibility of single nucleotide polymorphism genotyping with a single-probe by time-resolved Forster resonance energy transfer
Authors: <\/strong>Andreoni A, Bondani M, Nardo L.
Journal: <\/strong>Mol Cell Probes (2009): 119<\/p>\n\n\n\n

Photodynamic molecular beacon triggered by fibroblast activation protein on cancer-associated fibroblasts for diagnosis and treatment of epithelial cancers
Authors: <\/strong>Lo PC, Chen J, Stefflova K, Warren MS, Navab R, B and archi B, Mullins S, Tsao M, Cheng JD, Zheng G.
Journal: <\/strong>J Med Chem (2009): 358<\/p>\n\n\n\n

Rapid detection and quantification of Propionibacteriaceae
Authors: <\/strong>Goldschmidt P, Ferreira CC, Degorge S, Benallaoua D, Boutboul S, Laroche L, Batellier L, Chaumeil C.
Journal: <\/strong>Br J Ophthalmol (2009): 258<\/p>\n\n\n\n

Evaluation of tetramethylrhodamine and black hole quencher 1 labeled probes and five commercial amplification mixes in TaqMan real-time RT-PCR assays for respiratory pathogens
Authors: <\/strong>Yang GP, Erdman DD, Tondella ML, Fields BS.
Journal: <\/strong>J Virol Methods (2009): 288<\/p>\n\n\n\n

Design of FRET-TaqMan probes for multiplex real-time PCR using an internal positive control
Authors: <\/strong>Jothikumar P, Hill V, Narayanan J.
Journal: <\/strong>Biotechniques (2009): 519<\/p>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

En comparaci\u00f3n con las sondas Cy5, la familia TF5WS tiene una fluorescencia mucho m\u00e1s fuerte y una mayor fotoestabilidad siendo una opci\u00f3n superior al Cy5.<\/p>\n","protected":false},"featured_media":3282,"template":"","al_product-cat":[38],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3280"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":4,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3280\/revisions"}],"predecessor-version":[{"id":8185,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3280\/revisions\/8185"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3282"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3280"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3280"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}