{"id":3259,"date":"2023-01-12T17:10:31","date_gmt":"2023-01-12T23:10:31","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3259"},"modified":"2023-01-20T13:14:33","modified_gmt":"2023-01-20T19:14:33","slug":"calcein-am-ultrapure-grade","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/calcein-am-ultrapure-grade\/","title":{"rendered":"Calcein, AM *UltraPure grade*"},"content":{"rendered":"\n

La calce\u00edna AM pasa f\u00e1cilmente a trav\u00e9s de la membrana celular de las c\u00e9lulas viables debido a su mayor hidrofobicidad en comparaci\u00f3n con la calce\u00edna. El derivado de acetometoxi (AM) de la calce\u00edna (calce\u00edna AM) se usa ampliamente para marcar c\u00e9lulas vivas, ya que puede transportarse a trav\u00e9s de la membrana celular hacia las c\u00e9lulas vivas. Los grupos \u00e9ster AM enmascaran la parte de la mol\u00e9cula que quela el calcio. Al transportarse a las c\u00e9lulas vivas, las esterasas celulares cortan los grupos AM, la mol\u00e9cula se une al calcio dentro de la c\u00e9lula (lo que resulta en la adquisici\u00f3n de una fuerte fluorescencia verde) y queda atrapada en el interior. Como las c\u00e9lulas muertas carecen de esterasas, solo se marcan las c\u00e9lulas vivas. Esta caracter\u00edstica lo hace muy \u00fatil para probar la viabilidad celular y para el marcado de c\u00e9lulas a corto plazo. <\/p>\n\n\n\n

En comparaci\u00f3n con otros reactivos de marcado de c\u00e9lulas vivas (como BCECF-AM y diacetato de carboxifluoresce\u00edna), la calce\u00edna-AM es la sonda fluorescente m\u00e1s adecuada para te\u00f1ir c\u00e9lulas viables debido a su baja citotoxicidad. La calce\u00edna no afecta significativamente las funciones celulares como la proliferaci\u00f3n o la quimiotaxis de los linfocitos. Adem\u00e1s, los ensayos de viabilidad que utilizan calce\u00edna son fiables y se correlacionan bien con el ensayo est\u00e1ndar de liberaci\u00f3n de 51Cr.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22003<\/td>Calcein, AM *UltraPure grade*<\/td>1 mg<\/td><\/tr>
AAT-22004<\/td>Calcein, AM *UltraPure grade*<\/td>20 x 50 ug<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante, Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar  (< -15 \u00b0C), Minimizar la exposici\u00f3n a la luz<\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Cit\u00f3metro de Flujo<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n Laser<\/td>488 nm<\/td><\/tr>
Emisi\u00f3n Filtro<\/td>530\/30 nm<\/td><\/tr>
Especificaciones Instrumento<\/td>Canal FITC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Microscopio de Fluorescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>Juego de filtros FITC <\/td><\/tr>
Emisi\u00f3n<\/td>Juego de filtros FITC <\/td><\/tr>
Placa recomendada <\/td>Pared negra\/fondo transparente<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Lector de Microplacas de Fluorescencia<\/mark> <\/p>\n\n\n\n

Excitaci\u00f3n<\/td>490 nm<\/td><\/tr>
Emisi\u00f3n<\/td>525 nm<\/td><\/tr>
Corte<\/td>515 nm<\/td><\/tr>
Placa Recomendada<\/td>Pared negra\/fondo transparente<\/td><\/tr>
Especificaciones instrumento<\/td>Modo de lectura inferior<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro<\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Coeficiente de extinci\u00f3n (cm -1<\/sup> M -1<\/sup>)<\/td>81000<\/td><\/tr>
Excitaci\u00f3n (nm)<\/td>501<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>521<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Preparacion de solucion madre<\/strong><\/p>\n\n\n\n

A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

Calce\u00edna, AM UltraPure grade soluci\u00f3n madre<\/em>
Prepare una soluci\u00f3n madre de calce\u00edna AM de 2 a 5 mM en DMSO anhidro de alta calidad.
Nota: El detergente no i\u00f3nico Pluronic\u00ae F-127 se puede utilizar para aumentar la solubilidad acuosa de los \u00e9steres AM. En el buffer de tinci\u00f3n, la concentraci\u00f3n final de Pluronic\u00ae F-127 debe ser de aproximadamente 0,02 %. Se puede comprar una variedad de productos Pluronic\u00ae F-127 de AAT Bioquest. Evite el almacenamiento a largo plazo de esters AM en presencia de Pluronic\u00ae F-127.<\/p>\n\n\n\n

\n\n\n\n

Preparaci\u00f3n de soluci\u00f3n de trabajo<\/strong><\/p>\n\n\n\n

Calce\u00edna, AM UltraPure grade soluci\u00f3n de trabajo<\/em>
Prepare una soluci\u00f3n de trabajo de calce\u00edna AM de 1 a 10 \u00b5M en el buffer de su elecci\u00f3n (p. ej., buffer Hanks y Hepes). Para la mayor\u00eda de las l\u00edneas celulares, se recomienda calce\u00edna AM en la concentraci\u00f3n final de 4 a 5 \u00b5M. La concentraci\u00f3n exacta de indicadores necesarios para la carga de c\u00e9lulas debe determinarse emp\u00edricamente.
Nota:<\/strong> Si sus c\u00e9lulas contienen transportadores de aniones org\u00e1nicos, se puede agregar probenecid (1\u20132,5 mM) o sulfinpirazona (0,1\u20130,25 mM) a la soluci\u00f3n de trabajo para reducir las fugas de los indicadores desesterificados.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Calculadora<\/mark><\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark>
Tabla 1. Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de calce\u00edna, AM Grado ultrapuro<\/em> CAS 148504-34-1<\/em> a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>100.517 \u00b5L<\/td>502.583 \u00b5L<\/td>1.005 mL<\/td>5.026 mL<\/td>10.052 mL<\/td><\/tr>
5 mM<\/strong><\/td>20.103 \u00b5L<\/td>100.517 \u00b5L<\/td>201.033 \u00b5L<\/td>1.005 mL<\/td>2.01 mL<\/td><\/tr>
10 mM<\/strong><\/td>10.052 \u00b5L<\/td>50.258 \u00b5L<\/td>100.517 \u00b5L<\/td>502.583 \u00b5L<\/td>1.005 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Imagenes<\/mark><\/p>\n\n\n\n

<\/div>\n\n\n\n
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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1.<\/strong> Im\u00e1genes simult\u00e1neas de c\u00e9lulas HeLa vivas y apopt\u00f3ticas marcadas con calce\u00edna AM (n.\u00ba de cat. 22003) y conjugado de anexina V-iFluor\u00ae 647 (n.\u00ba de cat. 20074)<\/p>\n\n\n\n

<\/div>\n\n\n\n
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Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2<\/strong>. Im\u00e1genes de c\u00e9lulas HeLa Vivas te\u00f1idas con Calce\u00edna, AM (Cat.22003).<\/p>\n\n\n\n

<\/div>\n\n\n\n
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Fig. 3<\/figcaption><\/figure>\n\n\n\n

Figura 3.<\/strong> Estructura quimica para Calcein, AM *UltraPure grade* *CAS 148504-34-1*<\/p>\n\n\n\n

<\/div>\n\n\n\n

Formato Alternativo<\/mark><\/p>\n\n\n\n

Calcein, AM *CAS 148504-34-1*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Productos Relacionados<\/mark><\/p>\n\n\n\n

Nombre<\/td><\/tr>
Calcein Blue *CAS 54375-47-2*<\/a><\/td><\/tr>
NAADP-AM<\/a><\/td><\/tr>
BAPTA, AM *CAS 126150-97-8*<\/a><\/td><\/tr>
BAPTA, AM *UltraPure Grade* *CAS 126150-97-8*<\/a><\/td><\/tr>
EGTA AM *CAS 99590-86-0*<\/a><\/td><\/tr>
EGTA AM *10 mM DMSO solution*<\/a><\/td><\/tr>
BTC, AM *CAS 176767-94-5*<\/a><\/td><\/tr>
BCECF, AM<\/a><\/td><\/tr>
BCECF, AM *UltraPure grade*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Otros Productos Relacionados<\/mark><\/p>\n\n\n\n

Calcein Red\u2122 AM<\/a><\/td><\/tr>
Calcein Red\u2122 sodium salt<\/a><\/td><\/tr>
Calcein Deep Red\u2122<\/a><\/td><\/tr>
Calcein UltraBlue\u2122 AM<\/a><\/td><\/tr>
Calcein UltraBlue\u2122 sodium salt<\/a><\/td><\/tr>
Calcein *UltraPure Grade* *CAS 154071-48-4*<\/a><\/td><\/tr>
Calcein Blue, AM *CAS 168482-84-6*<\/a><\/td><\/tr>
Calcein Orange\u2122 sodium salt<\/a><\/td><\/tr>
Calcein Orange\u2122 diacetate<\/a><\/td><\/tr>
Calcein Deep Red\u2122 acetate<\/a><\/td><\/tr>
CytoCalcein\u2122 Violet 450 *Excited at 405 nm*<\/a><\/td><\/tr>
CytoCalcein\u2122 Violet 500 *Excited at 405 nm*<\/a><\/td><\/tr>
Calcein UltraGreen\u2122 AM<\/a><\/td><\/tr>
Calcein Deep Red\u2122 AM ester<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Ver todas las 31 bibliograf\u00edas:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Effervescent Atomizer as Novel Cell Spray Technology to Decrease the Gas-to-Liquid Ratio<\/a>
Authors: <\/strong>Thiebes, Anja Lena and Klein, Sarah and Zingsheim, Jonas and M{\\”o}ller, Georg H and G{\\”u}rzing, Stefanie and Reddemann, Manuel A and Behbahani, Mehdi and Jockenhoevel, Stefan and Cornelissen, Christian G
Journal: <\/strong>Pharmaceutics (2022): 2421<\/p>\n\n\n\n

Sensing of Physical Factors by Cells: Electric Field, Mechanical Forces, Physical Plasma and Light\u2014Importance for Tissue Regeneration<\/a>
Authors: <\/strong>Bielfeldt, Meike and Rebl, Henrike and Peters, Kirsten and Sridharan, Kaarthik and Staehlke, Susanne and Nebe, J Barbara
Journal: <\/strong>Biomedical Materials \\& Devices (2022): 1–16<\/p>\n\n\n\n

Genetic Engineering of Talaromyces marneffei to Enhance Siderophore Production and Preliminary Testing for Medical Application Potential<\/a>
Authors: <\/strong>Amsri, Artid and Srichairatanakool, Somdet and Teerawutgulrag, Aphiwat and Youngchim, Sirida and Pongpom, Monsicha
Journal: <\/strong>Journal of Fungi (2022): 1183<\/p>\n\n\n\n

Drug\/bioactive eluting chitosan composite foams for osteochondral tissue engineering<\/a>
Authors: <\/strong>Samie, Muhammad and Khan, Ather Farooq and Rahman, Saeed Ur and Iqbal, Haffsah and Yameen, Muhammad Arfat and Chaudhry, Aqif Anwar and Galeb, Hanaa A and Halcovitch, Nathan R and Hardy, John G
Journal: <\/strong>International Journal of Biological Macromolecules (2022)<\/p>\n\n\n\n

The effects of acute exercise and inflammation on immune function in early-stage prostate cancer<\/a>
Authors: <\/strong>Schauer, Tim and Djurhuus, Sissal Sigmundsd{\\’o}ttir and Simonsen, Casper and Brasso, Klaus and Christensen, Jesper Frank
Journal: <\/strong>Brain, behavior, \\& immunity-health (2022): 100508<\/p>\n\n\n\n

Highly Concentrated Nitrogen-Doped Carbon Nanotubes in Alginate–Gelatin 3D Hydrogels Enable in Vitro Breast Cancer Spheroid Formation<\/a>
Authors: <\/strong>Munguia-Lopez, Jose G and Jiang, Tao and Ferlatte, Audrey and Fajardo-Diaz, Juan L and Munoz-Sandoval, Emilio and Tran, Simon D and Kinsella, Joseph M
Journal: <\/strong>Advanced NanoBiomed Research (2021): 2100104<\/p>\n\n\n\n

CORRELATION BETWEEN CELL SURVIVAL AND ATOMIZATION CHARACTERISTICS IN AIR-ASSISTED BRONCHOSCOPIC SPRAYING<\/a>
Authors: <\/strong>G{\\”u}rzing, Stefanie and Klein, Sarah and M{\\”o}ller, Georg and Thiebes, Anja Lena and Cornelissen, Christian Gabriel and Reddemann, Manuel Armin
Journal: <\/strong>Atomization and Sprays (2021)<\/p>\n\n\n\n

Endoscopic atomization of mesenchymal stromal cells: in vitro study for local cell therapy of the lungs<\/a>
Authors: <\/strong>Thiebes, Anja Lena and Uhl, Franziska E and Hauser, Marie and Cornelissen, Christian G and Jockenhoevel, Stefan and Weiss, Daniel J
Journal: <\/strong>Cytotherapy (2021): 293–300<\/p>\n\n\n\n

A necroptotic-independent function of MLKL in regulating endothelial cell adhesion molecule expression<\/a>
Authors: <\/strong>Dai, Jialin and Zhang, Chonghe and Guo, Lin and He, Hao and Jiang, Kai and Huang, Yingying and Zhang, Xixi and Zhang, Haibing and Wei, Wu and Zhang, Yaoyang and others,
Journal: <\/strong>Cell Death \\& Disease (2020): 1–16<\/p>\n\n\n\n

Preparation of phospholipid-based polycarbonate urethanes for potential applications of blood-contacting implants<\/a>
Authors: <\/strong>Li, Peichuang and Cai, Wanhao and Li, Xin and Wang, Kebing and Zhou, Lei and You, Tianxue and Wang, Rui and Chen, Hang and Zhao, Yuancong and Wang, Jin and others,
Journal: <\/strong>Regenerative biomaterials (2020): 491–504<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 84 referencias:<\/mark>\u00a0<\/mark><\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development<\/a>
Authors: <\/strong>Li, Deng and Xiang, Xiaocong and Yang, Fei and Xiao, Dongqin and Liu, Kang and Chen, Zhu and Zhang, Ruolan and Feng, Gang
Journal: <\/strong>Biochemical and Biophysical Research Communications (2017)<\/p>\n\n\n\n

Localized functional chemical stimulation of TE 671 cells cultured on nanoporous membrane by calcein and acetylcholine
Authors: <\/strong>Zibek S, Stett A, Koltay P, Hu M, Zengerle R, Nisch W, Stelzle M.
Journal: <\/strong>Biophys J. (2006)<\/p>\n\n\n\n

A vaccination and challenge model using calcein marked fish
Authors: <\/strong>Klesius PH, Evans JJ, Shoemaker CA, Pasnik DJ.
Journal: <\/strong>Fish Shellfish Immunol (2006): 20<\/p>\n\n\n\n

Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viability and aging
Authors: <\/strong>Bratosin D, Mitrofan L, Palii C, Estaquier J, Montreuil J.
Journal: <\/strong>Cytometry A (2005): 78<\/p>\n\n\n\n

Cytotoxic effects of 100 reference compounds on Hep G2 and HeLa cells and of 60 compounds on ECC-1 and CHO cells. I mechanistic assays on ROS, glutathione depletion and calcein uptake
Authors: <\/strong>Schoonen WG, Westerink WM, de Roos JA, Debiton E.
Journal: <\/strong>Toxicol In Vitro (2005): 505<\/p>\n\n\n\n

Calcein AM release-based cytotoxic cell assay for fish leucocytes
Authors: <\/strong>Iwanowicz LR, Densmore CL, Ottinger CA.
Journal: <\/strong>Fish Shellfish Immunol (2004): 127<\/p>\n\n\n\n

Calcein-AM is a detector of intracellular oxidative activity
Authors: <\/strong>Uggeri J, Gatti R, Belletti S, Sc and roglio R, Corradini R, Rotoli BM, Orl and ini G., undefined
Journal: <\/strong>Histochem Cell Biol (2004): 499<\/p>\n\n\n\n

Comparison of the usefulness of the MTT, ATP, and calcein assays to predict the potency of cytotoxic agents in various human cancer cell lines
Authors: <\/strong>Mueller H, Kassack MU, Wiese M.
Journal: <\/strong>J Biomol Screen (2004): 506<\/p>\n\n\n\n

In vitro assay of mineralized-tissue formation on titanium using fluorescent staining with calcein blue
Authors: <\/strong>Goto T, Kajiwara H, Yoshinari M, Fukuhara E, Kobayashi S, Tanaka T.
Journal: <\/strong>Biomaterials (2003): 3885<\/p>\n\n\n\n

The effects of calcium chloride and sodium chloride on the electroporation-mediated skin permeation of fluorescein isothiocyanate (FITC)-dextrans in vitro
Authors: <\/strong>Tokudome Y, Sugibayashi K.
Journal: <\/strong>Biol Pharm Bull (2003): 1508<\/p>\n\n\n\n

<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

Multiplexing Cell Proliferation and Cytotoxicity Assays Using Calcein Red and CytoCalceins<\/a>
Annexin V<\/a>
Calcein<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Calceina AM Grado Ultrapuro *CAS 148504-34-1*. En comparaci\u00f3n con otros reactivos del marcado de c\u00e9lulas vivas (como BCECF-AM y diacetato de carboxifluoresce\u00edna), la calce\u00edna-AM es la sonda fluorescente m\u00e1s adecuada para te\u00f1ir c\u00e9lulas viables debido a su baja citotoxicidad.<\/p>\n","protected":false},"featured_media":3264,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3259"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":7,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3259\/revisions"}],"predecessor-version":[{"id":3719,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3259\/revisions\/3719"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3264"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3259"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3259"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}