{"id":3233,"date":"2023-01-11T17:52:07","date_gmt":"2023-01-11T23:52:07","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3233"},"modified":"2023-01-20T13:25:24","modified_gmt":"2023-01-20T19:25:24","slug":"readiuse-stayright-purple-hrp-chromogen-premixed-with-hydrogen-peroxide","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/readiuse-stayright-purple-hrp-chromogen-premixed-with-hydrogen-peroxide\/","title":{"rendered":"ReadiUse\u2122 Stayright\u2122 Purple *HRP Chromogen Premixed with Hydrogen Peroxide*\u00a0\u00a0"},"content":{"rendered":"\n

La 3,3′-diaminobencidina (DAB) se ha utilizado como el crom\u00f3geno IHC m\u00e1s utilizado porque es econ\u00f3mico y sensible para aplicaciones de rutina. Sin embargo, se ha demostrado que DAB es mutag\u00e9nico y peligroso para los trabajadores de laboratorio y el medio ambiente. Para abordar este problema, AAT Bioquest ha desarrollado este novedoso Stayright\u2122 Purple como un crom\u00f3geno IHC significativamente m\u00e1s seguro que DAB. Adem\u00e1s, Stayright\u2122 Purple proporciona un m\u00e9todo r\u00e1pido y sencillo para desarrollar una mancha p\u00farpura intensa y limpia en presencia de HRP con alta sensibilidad como DAB. El sustrato Stayright\u2122 Purple HRP listo para usar, no muestra efectos mutag\u00e9nicos con una citotoxicidad m\u00ednima.<\/p>\n\n\n\n

El sustrato de peroxidasa p\u00farpura (HRP) ReadiUse\u2122 Stayright\u2122 es adecuado para su uso en m\u00e9todos de tinci\u00f3n de inmunohistoqu\u00edmica (IHC) e hibridaci\u00f3n in situ (ISH) basados \u200b\u200ben peroxidasa (HRP). El sustrato es una soluci\u00f3n premezclada estable que contiene per\u00f3xido de hidr\u00f3geno, por lo que se eliminan todos los pasos de mezcla y est\u00e1 listo para usar. Tras la oxidaci\u00f3n inducida por HRP, Stayright\u2122 Purple forma un producto precipitante insoluble de color p\u00farpura en el sitio objetivo de su ensayo. El producto final p\u00farpura es insoluble en solventes org\u00e1nicos y medios de montaje org\u00e1nicos, por lo que la mancha p\u00farpura distintiva se puede mantener a pesar de los pasos de manipulaci\u00f3n regulares de deshidrataci\u00f3n y colocaci\u00f3n de cubreobjetos.\u00a0 <\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-45900<\/td>ReadiUse\u2122 Stayright\u2122 Purple *HRP Chromogen Premixed with Hydrogen Peroxide*<\/td>5 ml<\/td><\/tr>
AAT-45901<\/td>ReadiUse\u2122 Stayright\u2122 Purple *HRP Chromogen Premixed with Hydrogen Peroxide*<\/td>50 ml<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Refrigerar (2-8 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Microscopio de luz blanca<\/mark><\/p>\n\n\n\n

<\/div>\n\n\n\n

MUESTRA DE PROTOCOLO EXPERIMENTAL
1. Cubra la secci\u00f3n con la soluci\u00f3n Stayright\u2122 Purple lista para usar. Incubar a temperatura ambiente durante 5-15 minutos.
2. Sumerja los portaobjetos en dH2O para detener el desarrollo del color y controlar la intensidad de la tinci\u00f3n. Si la intensidad de la tinci\u00f3n no es lo suficientemente brillante, se necesita una incubaci\u00f3n m\u00e1s prolongada. Se puede volver a aplicar la soluci\u00f3n Stayright\u2122 Purple para continuar con el desarrollo.
3. Lavar con dH2O durante 5-10 minutos.
4. Use una contratinci\u00f3n deseada si es necesario.
5. Deshidratar con etanol y montar permanentemente en medio de montaje permanente org\u00e1nico.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Imagenes<\/mark><\/p>\n\n\n\n

\"\"
Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1. <\/strong>Detecci\u00f3n inmunohistoqu\u00edmica de EpCAM en tejido de adenocarcinoma de pulm\u00f3n FFPE. Las secciones de tejido se incubaron con IgG anti-conejo de cabra conjugada con poli-HRP y luego se revelaron con Stayright\u2122 Purple (izquierda) o DAB (derecha), respectivamente. Las c\u00e9lulas tambi\u00e9n se contrastaron con hematoxilina. Stayright\u2122 Purple genera una mancha intensa con alta sensibilidad y resoluci\u00f3n clara similar a DAB.<\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"
Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2.<\/strong> Se fijaron muestras de tejido de adenocarcinoma de pulm\u00f3n humano y se ti\u00f1eron para EpCAM. Las secciones de tejido se incubaron con un anticuerpo monoclonal de conejo anti-EpCAM (izquierda) o isotipo IgG como control negativo (derecha). A continuaci\u00f3n, la muestra se incub\u00f3 con IgG anti-conejo de cabra conjugada con poli-HRP y la se\u00f1al se desarroll\u00f3 con soluci\u00f3n Stayright\u2122 Purple. Stayright\u2122 Purple genera una mancha de color p\u00farpura limpia sin fondo.<\/p>\n\n\n\n

<\/div>\n\n\n\n
\"\"
Fig. 3<\/figcaption><\/figure>\n\n\n\n

Figura 3.<\/strong> Comparaci\u00f3n de los efectos citot\u00f3xicos entre Stayright\u2122 Purple y DAB en la proliferaci\u00f3n de c\u00e9lulas HeLa. Se utiliz\u00f3 el ensayo WST-8 de AAT (n.\u00b0 de cat\u00e1logo 22770) para determinar la viabilidad celular despu\u00e9s del tratamiento con aproximadamente 0, 2, 6, 18, 55, 100, 150, 200, 400, 500, 800 o 1000 \u03bcg\/mL de Stayright\u2122 P\u00farpura o DAB durante 24 horas, respectivamente. Se determinaron los valores IC50 y se demostr\u00f3 que Stayright\u2122 Purple es una alternativa significativamente m\u00e1s segura que DAB<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Relacionados<\/mark><\/p>\n\n\n\n

ReadiUse\u2122 Preactivated APC-iFluor\u00ae 700 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-iFluor\u00ae 750 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 647 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 750 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 ABTS Substrate Solution *<\/a>O<\/a>ptimized for ELISA Assays with HRP Conjugates*<\/a><\/td><\/tr>
ReadiUse\u2122 TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*<\/a><\/td><\/tr>
ReadiUse\u2122 hydrogen peroxide solution *50 mM calibrated and stabilized solution*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated HRP NHS ester<\/a><\/td><\/tr>
ReadiUse\u2122 NADPH Regenerating Kit<\/a><\/td><\/tr>
ReadiUse\u2122 NADP Regenerating Kit<\/a><\/td><\/tr>
ReadiUse\u2122 TCA Deproteinization Sample Preparation Kit<\/a><\/td><\/tr>
ReadiUse\u2122 microscope mounting solution<\/a><\/td><\/tr>
ReadiUse\u2122 4% formaldehyde fixation solution<\/a><\/td><\/tr>
ReadiUse\u2122 mammalian cell lysis buffer *5X*<\/a><\/td><\/tr>
ReadiUse\u2122 probenecid, sodium salt *Water-soluble*<\/a><\/td><\/tr>
ReadiUse\u2122 probenecid *25 mM stabilized aqueous solution*<\/a><\/td><\/tr>
ReadiUse\u2122 CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy5.5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy7 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Texas Red Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-Cy5.5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-Cy7 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 TCEP removal solution<\/a><\/td><\/tr>
ReadiUse\u2122 WST-8 *50 mM aqueous solution*<\/a><\/td><\/tr>
ReadiUse\u2122 Rapid Luminometric ATP Assay Kit<\/a><\/td><\/tr>
ReadiUse\u2122 PE [R-Phycoerythrin] *Ammonium Sulfate-Free*<\/a><\/td><\/tr>
ReadiUse\u2122 CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 594 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Cell Detaching Buffer<\/a><\/td><\/tr>
ReadiUse\u2122 DRAQ5 Staining Solution *5 mM in Water*<\/a><\/td><\/tr>
ReadiUse\u2122 Staurosporine * 1 mM DMSO stock solution*<\/a><\/td><\/tr>
ReadiUse\u2122 Bio-Gel P-6 spin column<\/a><\/td><\/tr>
ReadiUse\u2122 bacterial cell lysis buffer *5X*<\/a><\/td><\/tr>
ReadiUse\u2122 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 700 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 dNTP Mix *10 mM*<\/a><\/td><\/tr>
ReadiUse\u2122 Viral RNA Lysis Buffer<\/a><\/td><\/tr>
ReadiUse\u2122 Tyramide (TSA)\/Styramide (PSA) Optimized Reaction Buffer<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PerCP<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PerCP-Cy5.5 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-iFluor\u00ae 660 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 10KD Spin Filter<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-iFluor\u00ae 800 Tandem<\/a><\/td><\/tr>
ReadiUse\u2122 1 Kb Plus DNA Ladder<\/a><\/td><\/tr>
ReadiUse\u2122 GeneRuler 1 kb DNA Ladder<\/a><\/td><\/tr>
ReadiUse\u2122 100 bp DNA Ladder<\/a><\/td><\/tr>
ReadiUse\u2122 ddNTP Terminator Mix *10 mM*<\/a><\/td><\/tr>
ReadiUse\u2122 dNTP Mix Set *10 mM PCR Grade*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated HRP maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Disposable PD-10 Desalting Column<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC Maleimide [Activated Allophycocyanin]<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE Maleimide [Activated R-Phycoerythrin]<\/a><\/td><\/tr>
ReadiUse\u2122 6-Color Human TBNK Antibody Kit *Dry Reagent Format*<\/a><\/td><\/tr>
ReadiUse\u2122 MTS Reagent *50 mM aqueous solution*<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Texas Red Maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy5 Maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy5.5 Maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PE-Cy7 Maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-AF700 Maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-Cy5.5 Maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated APC-Cy7 Maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PerCP Maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PerCP-Cy5.5 Maleimide<\/a><\/td><\/tr>
ReadiUse\u2122 Preactivated PerCP-Cy7 Maleimide<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00edas<\/mark><\/p>\n\n\n\n

Ver todas las 26 bibliograf\u00edas:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Epigenetic suppression of SLFN11 in germinal center B-cells during B-cell development<\/a>
Authors: <\/strong>Moribe, Fumiya and Nishikori, Momoko and Takashima, Tsuyoshi and Taniyama, Daiki and Onishi, Nobuyuki and Arima, Hiroshi and Sasanuma, Hiroyuki and Akagawa, Remi and Elloumi, Fathi and Takeda, Shunichi and others,
Journal: <\/strong>PloS one (2021): e0237554<\/p>\n\n\n\n

3,3′-diaminobenzidine (DAB)-H2O2-HRP voltammetric enzyme-linked immunoassay for the detection of carcionembryonic antigen
Authors: <\/strong>Zhang, S., Yang, J., Lin, J.
Journal: <\/strong>Bioelectrochemistry (2008): 47-52<\/p>\n\n\n\n

HistoGreen: a new alternative to 3,3′-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) as a peroxidase substrate in immunohistochemistry?
Authors: <\/strong>Thomas, M. A., Lemmer, B.
Journal: <\/strong>Brain Res Brain Res Protoc (2005): 107-18<\/p>\n\n\n\n

Stability and solubility of 3,3′-diaminobenzidine (DAB)
Authors: <\/strong>Kiernan, J. A.
Journal: <\/strong>Biotech Histochem (2003): 135<\/p>\n\n\n\n

Safe diaminobenzidine (DAB) disposal
Authors: <\/strong>Horn, H.
Journal: <\/strong>Biotech Histochem (2002): 229<\/p>\n\n\n\n

Non-fluorescent chromosome painting using the peroxidase\/diaminobenzidine (DAB) reaction
Authors: <\/strong>K, undefined and a, R., Suzuki, M., Minamihisamatsu, M., Furukawa, A., Odaka, T., Hayata, I.
Journal: <\/strong>Int J Radiat Biol (1998): 529-33<\/p>\n\n\n\n

Modified cerium-based and Gomori-based cerium methods for light microscopic phosphatase histochemistry: the cerium-perhydroxide-diaminobenzidine-nickel (Ce-H2O2-DAB-Ni and Ce\/Ce-H2O2-DAB-Ni) two-step procedures
Authors: <\/strong>Halbhuber, K. J., Feuerstein, H., Moller, U., Klinger, M.
Journal: <\/strong>Acta Histochem (1992): 87-103<\/p>\n\n\n\n

Employment of merocyanine 540 fluorescence to form diaminobenzidine (DAB) oxidation product: a photoconversion method for the visualization of erythrocyte membrane fluidity for light and electron microscopy
Authors: <\/strong>Oehring, H., Halbhuber, K. J.
Journal: <\/strong>Acta Histochem (1991): 127-34<\/p>\n\n\n\n

The cerium perhydroxide-diaminobenzidine (Ce-H2O2-DAB) procedure. New methods for light microscopic phosphatase histochemistry and immunohistochemistry
Authors: <\/strong>Halbhuber, K. J., Gossrau, R., Moller, U., Hulstaert, C. E., Zimmermann, N., Feuerstein, H.
Journal: <\/strong>Histochemistry (1988): 289-97<\/p>\n\n\n\n

Copper-H2O2 oxidation strikingly improves silver intensification of the nickel-diaminobenzidine (Ni-DAB) end-product of the peroxidase reaction
Authors: <\/strong>Gallyas, F., Merchenthaler, I.
Journal: <\/strong>J Histochem Cytochem (1988): 807-10<\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

ReadiUse\u2122 Stayright\u2122 Purple *HRP Chromogen premezclado con per\u00f3xido de hidr\u00f3geno* . Stayright\u2122 Purple proporciona un m\u00e9todo r\u00e1pido y sencillo para desarrollar una mancha p\u00farpura intensa y limpia en presencia de HRP con alta sensibilidad como DAB. <\/p>\n","protected":false},"featured_media":3239,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3233"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":5,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3233\/revisions"}],"predecessor-version":[{"id":3723,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3233\/revisions\/3723"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3239"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3233"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3233"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}