{"id":3229,"date":"2023-01-11T17:10:46","date_gmt":"2023-01-11T23:10:46","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3229"},"modified":"2023-01-20T13:36:13","modified_gmt":"2023-01-20T19:36:13","slug":"5-dr110-succinimidyl-ester","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/5-dr110-succinimidyl-ester\/","title":{"rendered":"5-dR110, succinimidyl ester\u00a0\u00a0"},"content":{"rendered":"\n

En comparaci\u00f3n con los reactivos de marcaje con fluoresce\u00edna como FITC y FAM, los reactivos 5-dR110 [5-carboxi-4,7-diclororodamina 110] proporcionan bioconjugados m\u00e1s fotoestables e independientes del pH que tienen el m\u00e1ximo de absorci\u00f3n alrededor de la longitud de onda de excitaci\u00f3n preferida de 488 nm. Son reactivos alternativos fotoestables superiores a FITC y FAM. La 5-carboxi-4,7-diclororodamina (dR110) es uno de los cuatro colorantes cr\u00edticos de 4,7-dicloro-rodamina utilizados como terminadores BigDye para la secuenciaci\u00f3n de ADN en combinaci\u00f3n con d6G, dTMR y dROX. ABI PRISM\u00ae BigDye\u2122 Terminator Cycle Sequencing es el m\u00e9todo cl\u00e1sico para la secuenciaci\u00f3n de ADN. 5-dR110, el succinimidil \u00e9ster es el bloque de construcci\u00f3n m\u00e1s conveniente para preparar el color dR110 del juego de fluor\u00f3foros de secuenciaci\u00f3n BigDye Sanger.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-317<\/td>5-dR110, succinimidyl ester <\/td>5 mg<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

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Importante: Solo para uso en investigaci\u00f3n (RUO). Almacenamiento: Congelar (<-15 \u00b0C). Minimizar la exposici\u00f3n a la luz.<\/p>\n\n\n\n

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Propiedades f\u00edsicas<\/mark><\/p>\n\n\n\n

Peso Molecular <\/td>540.31<\/td><\/tr>
Disolvente<\/td>DMSO<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Calculadora\u00a0<\/mark>\u00a0\u00a0<\/p>\n\n\n\n

Preparaci\u00f3n de la soluci\u00f3n de stock com\u00fan<\/mark><\/p>\n\n\n\n

Volumen de DMSO necesario para reconstituir la masa espec\u00edfica de 5-dR110, succinimidil \u00e9ster a la concentraci\u00f3n dada. Tenga en cuenta que el volumen es solo para preparar la soluci\u00f3n madre. Consulte el protocolo experimental de muestra para conocer los buffers experimentales\/fisiol\u00f3gicos apropiados.<\/p>\n\n\n\n

<\/td>0.1 mg<\/strong><\/td>0.5 mg<\/strong><\/td>1 mg<\/strong><\/td>5 mg<\/strong><\/td>10 mg<\/strong><\/td><\/tr>
1 mM<\/strong><\/td>185.079 \u00b5L<\/td>925.395 \u00b5L<\/td>1.851 mL<\/td>9.254 mL<\/td>18.508 mL<\/td><\/tr>
5 mM<\/strong><\/td>37.016 \u00b5L<\/td>185.079 \u00b5L<\/td>370.158 \u00b5L<\/td>1.851 mL<\/td>3.702 mL<\/td><\/tr>
10 mM<\/strong><\/td>18.508 \u00b5L<\/td>92.539 \u00b5L<\/td>185.079 \u00b5L<\/td>925.395 \u00b5L<\/td>1.851 mL<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Imagen<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1. <\/strong>Estructura quimica para 5-dR110, succinimidyl ester.<\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Nombre<\/td><\/tr>
5-OG488 succinimidyl ester [equivalent to Oregon Green\u00ae 488 carboxylic acid, succinimidyl ester, 5-isomer ]<\/a><\/td><\/tr>
5-dR6G, succinimidyl ester<\/a><\/td><\/tr>
5-dTMR, succinimidyl ester<\/a><\/td><\/tr>
5-dROX, succinimidyl ester<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Application Notes<\/mark><\/p>\n\n\n\n

A New Protein Crosslinking Method for Labeling and Modifying Antibodies<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
FITC (Fluorescein isothiocyanate)<\/a>
Fluorescein isothiocyanate (FITC)<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

FAQ<\/mark><\/p>\n\n\n\n

Are any of the cyanine dyes infrared?<\/a>
Are coumarin dyes pH sensitive?<\/a>
Are there any alternatives to BrdU (Bromodeoxyuridine)?<\/a>
Are there any alternatives to Cy5?<\/a>
Are there any alternatives to indocyanine green (ICG)?<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Reactivo de marcaje con fluoresce\u00edna 5-dR110, succinimidyl ester. 5-dR110. Es el bloque de construcci\u00f3n m\u00e1s conveniente para preparar el color dR110 del juego de fluor\u00f3foros de secuenciaci\u00f3n BigDye Sanger.<\/p>\n","protected":false},"featured_media":3230,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3229"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":3,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3229\/revisions"}],"predecessor-version":[{"id":3725,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3229\/revisions\/3725"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3230"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3229"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3229"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}