{"id":3212,"date":"2023-01-11T12:03:02","date_gmt":"2023-01-11T18:03:02","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3212"},"modified":"2023-01-20T13:47:56","modified_gmt":"2023-01-20T19:47:56","slug":"amplite-luciferase-reporter-gene-assay-kit-bright-glow","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-luciferase-reporter-gene-assay-kit-bright-glow\/","title":{"rendered":"Amplite\u00ae Luciferase Reporter Gene Assay Kit *Bright Glow*"},"content":{"rendered":"\n

Los genes indicadores comunes incluyen beta-galactosidasa, beta-glucuronidasa y luciferasa. Las ventajas de un ensayo de luciferasa son la alta sensibilidad, la ausencia de actividad de luciferasa dentro de la mayor\u00eda de los tipos de c\u00e9lulas, el amplio rango din\u00e1mico, la rapidez y el bajo costo. El gen indicador m\u00e1s vers\u00e1til y com\u00fan es la luciferasa de la luci\u00e9rnaga norteamericana Photinus pyralis. La prote\u00edna no requiere modificaci\u00f3n postraduccional para la actividad enzim\u00e1tica. Ni siquiera es t\u00f3xico en alta concentraci\u00f3n (in vivo) y puede usarse en c\u00e9lulas procariotas y eucariotas. La luciferasa de luci\u00e9rnaga cataliza la oxidaci\u00f3n bioluminiscente de la luciferina en presencia de ATP, magnesio y ox\u00edgeno. <\/p>\n\n\n\n

Este kit de ensayo del gen reportero de luciferasa Amplite\u00ae utiliza una formulaci\u00f3n luminog\u00e9nica patentada para cuantificar la actividad de la luciferasa en c\u00e9lulas vivas y extractos celulares. Nuestra formulaci\u00f3n genera un producto luminiscente que da una fuerte luminiscencia al interactuar con la luciferasa. El kit proporciona todos los componentes esenciales con nuestro protocolo de ensayo optimizado de “mezclar y leer” que es compatible con los instrumentos de manejo de l\u00edquidos HTS. Tiene una sensibilidad extremadamente alta y se puede utilizar para los ensayos que requieren una sensibilidad exigente.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-12518<\/td>Amplite\u00ae Luciferase Reporter Gene Assay Kit *Bright Glow*<\/td>1 placa<\/td><\/tr>
AAT-12519<\/td>Amplite\u00ae Luciferase Reporter Gene Assay Kit *Bright Glow*<\/td>10 placas<\/td><\/tr>
AAT-12520<\/td>Amplite\u00ae Luciferase Reporter Gene Assay Kit *Bright Glow*<\/td>100 placas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de microplacas de luminiscencia<\/mark> <\/p>\n\n\n\n

Placa recomendada<\/td>Blanca s\u00f3lida <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: Luciferase Sensor (Light-sensitive)<\/td>1 botella<\/td><\/tr>
Componente B: Buffer de ensayo <\/td>1 botella (10 mL)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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PREPARACION CELULAR<\/p>\n\n\n\n

Para obtener guia sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite: https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n\n\n\n

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PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

1. Para el # de cat\u00e1logo 12518, transfiera todo el contenido del buffer de reacci\u00f3n (componente B) a la botella del sensor de luciferasa (componente A) y mezcle bien para que la soluci\u00f3n del sensor de luciferasa funcione.<\/p>\n\n\n\n

2. Para el # de cat. 12519, agregue 10 ml de buffer de reacci\u00f3n (componente B) y para el # de cat. 12520, agregue 100 ml de buffer de reacci\u00f3n (componente B) en la botella del sensor de luciferasa (componente A) y mezcle bien. Luego, vuelva a transferir la soluci\u00f3n resultante a la botella de buffer de reacci\u00f3n (componente B). Se necesitan m\u00faltiples lavados para transferir completamente el contenido. Nota: La soluci\u00f3n de trabajo del sensor de luciferasa reconstituida no es estable. Proteger de la luz.<\/p>\n\n\n\n

<\/p>\n<\/div><\/div>\n\n\n\n

<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1<\/strong>. Cin\u00e9tica de reacci\u00f3n de las c\u00e9lulas CHO-V2R-Luc utilizando el kit de ensayo de gen informador de luciferasa Amplite\u00ae. Las c\u00e9lulas CHO transfectadas de forma estable con el gen pCRE-luciferasa y el receptor 2 de vasopresina humana (V2R) se sembraron en una placa costar de fondo transparente\/pared blanca de 384 pocillos a 15.000 c\u00e9lulas\/pocillo\/25 \u00b5l. Luego, las c\u00e9lulas se trataron con 100 nM de vasopresina en una incubadora de CO2 al 5 % a 37 \u00b0C durante 4 horas. Se a\u00f1adieron al pocillo 25 \u00b5l de soluci\u00f3n de ensayo de luciferasa. Los datos cin\u00e9ticos se tomaron cada 30 minutos durante un m\u00e1ximo de 3 horas con un lector de placas NOVOstar (BMG Labtech). La se\u00f1al de luciferasa inducida por vasopresina es estable durante m\u00e1s de 3 horas.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/p>\n\n\n\n

Discovery of small molecule agonists of the Relaxin Family Peptide Receptor 2<\/a>
Authors: <\/strong>Esteban-Lopez, Maria and Wilson, Kenneth J and Myhr, Courtney and Kaftanovskaya, Elena M and Henderson, Mark J and Southall, Noel T and Xu, Xin and Wang, Amy and Hu, Xin and Barnaeva, Elena and others,
Journal: <\/strong>Communications biology (2022): 1–12<\/p>\n\n\n\n

AtHSPR is involved in GA-and light intensity-mediated control of flowering time and seed set in Arabidopsis<\/a>
Authors: <\/strong>Yang, Tao and Sun, Yan and Wang, Yongli and Zhou, Lina and Chen, Mengya and Bian, Zhiyuan and Lian, Yuke and Xuan, Lijuan and Yuan, Guoqiang and Wang, Xinyu and others,
Journal: <\/strong>Journal of Experimental Botany (2020)<\/p>\n\n\n\n

AtHSPR is involved in GA-and light intensity-mediated control of flowering time and seed set in Arabidopsis<\/a>
Authors: <\/strong>Yang, Tao and Sun, Yan and Wang, Yongli and Zhou, Lina and Chen, Mengya and Bian, Zhiyuan and Lian, Yuke and Xuan, Lijuan and Yuan, Guoqiang and Wang, Xinyu and others,
Journal: <\/strong>Journal of experimental botany (2020): 3543–3559<\/p>\n\n\n\n

LncRNA TUBA4B functions as a competitive endogenous RNA to inhibit gastric cancer progression by elevating PTEN via sponging miR-214 and miR-216a\/b<\/a>
Authors: <\/strong>Guo, Jianbo and Li, Yan and Duan, He and Yuan, Lu
Journal: <\/strong>Cancer Cell International (2019): 156<\/p>\n\n\n\n

Microwave ablation-assisted liver gene transfection in rats<\/a>
Authors: <\/strong>Jiang, Ruoyu and Meng, Lingkai and Sun, Longhao and He, Xianghui and Liang, Xiaoyu and Zhang, Jie and Zhang, Zhixiang
Journal: <\/strong>International Journal of Hyperthermia (2016): 666–672<\/p>\n\n\n\n

Identification of compounds that modulate retinol signaling using a cell-based qHTS assay<\/a>
Authors: <\/strong>Chen, Yanling and Sakamuru, Srilatha and Huang, Ruili and Reese, David H and Xia, Menghang
Journal: <\/strong>Toxicology in Vitro (2016): 287–296<\/p>\n\n\n\n

Activation of relaxin family receptor 1 from different mammalian species by relaxin peptide and small-molecule agonist ML290<\/a>
Authors: <\/strong>Huang, Zaohua and Myhr, Courtney and Bathgate, Ross AD and Ho, Brian A and Bueno, Amaya and Hu, Xin and Xiao, Jingbo and Southall, Noel and Barnaeva, Elena and Agoulnik, Irina U and others, undefined
Journal: <\/strong>Frontiers in endocrinology (2015)<\/p>\n\n\n\n

Neuroprotective effect of schizandrin A on oxygen and glucose deprivation\/reperfusion-induced cell injury in primary culture of rat cortical neurons<\/a>
Authors: <\/strong>Wang, Cai-Ping and Li, Gui-Cai and Shi, Yun-Wei and Zhang, Xiao-Chuan and Li, Jian-Long and Wang, Zhi-Wei and Ding, Fei and Liang, Xin-Miao
Journal: <\/strong>Journal of physiology and biochemistry (2014): 735–747<\/p>\n\n\n\n

Discovery of ML367, inhibitor of ATAD5 stabilization<\/a>
Authors: <\/strong>Rohde, Jason M and Rai, Ganesha and Choi, Yong Jun and Sakamuru, Srilatha and Fox, Jennifer T and Huang, Ruili and Xia, Menghang and Myung, Kyungjae and Boxer, Matthew B and Maloney, David J
Journal: <\/strong>(2013)<\/p>\n\n\n\n

<\/div>\n\n\n\n

Referencias<\/mark><\/p>\n\n\n\n

Ver todas las 52 referencias:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

In vivo bioluminescence tumor imaging of RGD peptide-modified adenoviral vector encoding firefly luciferase reporter gene
Authors: <\/strong>Niu G, Xiong Z, Cheng Z, Cai W, Gambhir SS, Xing L, Chen X.
Journal: <\/strong>Mol Imaging Biol (2007): 126<\/p>\n\n\n\n

Creation and Characterization of a Doxycycline-Inducible Mouse Model of Thyroid-Targeted RET\/PTC1 Oncogene and Luciferase Reporter Gene Coexpression
Authors: <\/strong>Knostman KA, Venkateswaran A, Zimmerman B, Capen CC, Jhiang SM.
Journal: <\/strong>Thyroid (2007): 1181<\/p>\n\n\n\n

Establishment of evaluation method for siRNA delivery using stable cell line carrying the luciferase reporter gene
Authors: <\/strong>Iijima A, Hachisu R, Kobayashi H, Hashimoto K, Asano D, Kikuchi H.
Journal: <\/strong>Biol Pharm Bull (2007): 1844<\/p>\n\n\n\n

Non-invasive imaging of firefly luciferase reporter gene expression using bioluminescence imaging in human prostate cancer models
Authors: <\/strong>Li H, Li JZ, Helm GA, Pan D.
Journal: <\/strong>Biotechnol Appl Biochem (2007): 179<\/p>\n\n\n\n

Analysis of rhythmic gene expression in adult Drosophila using the firefly luciferase reporter gene
Authors: <\/strong>Stanewsky R., undefined
Journal: <\/strong>Methods Mol Biol (2007): 131<\/p>\n\n\n\n

Reporter gene assay against lipophilic chemicals based on site-specific genomic recombination of a nuclear receptor gene, its response element, and a luciferase reporter gene within a stable HeLa cell line
Authors: <\/strong>Mori T, Saito F, Yoshino T, Takeyama H, Matsunaga T.
Journal: <\/strong>Biotechnol Bioeng. (2007)<\/p>\n\n\n\n

A GUS\/luciferase fusion reporter for plant gene trapping and for assay of promoter activity with luciferin-dependent control of the reporter protein stability
Authors: <\/strong>Koo J, Kim Y, Kim J, Yeom M, Lee IC, Nam HG.
Journal: <\/strong>Plant Cell Physiol (2007): 1121<\/p>\n\n\n\n

Functional characterization of cell lines for high-throughput screening of human neuromedin U receptor subtype 2 specific agonists using a luciferase reporter gene assay
Authors: <\/strong>Li X, Shen F, Zhang Y, Zhu J, Huang L, Shi Q.
Journal: <\/strong>Eur J Pharm Biopharm (2007): 284<\/p>\n\n\n\n

Adaptation of a luciferase gene reporter and lac expression system to Borrelia burgdorferi
Authors: <\/strong>Blevins JS, Revel AT, Smith AH, Bachlani GN, Norgard MV.
Journal: <\/strong>Appl Environ Microbiol (2007): 1501<\/p>\n\n\n\n

Novel luciferase reporter system for in vitro and organ-specific monitoring of differential gene expression in Listeria monocytogenes
Authors: <\/strong>Bron PA, Monk IR, Corr SC, Hill C, Gahan CG.
Journal: <\/strong>Appl Environ Microbiol (2006): 2876<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Library of Well-Defined and Water-Soluble Poly(alkyl phosphonate)s with Adjustable Hydrolysis<\/a>
Acetylcholinesterase Inhibitory Activity of Pigment Echinochrome A<\/a>
Ameliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer’s Disease<\/a>
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults<\/a>
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Kit de ensayo para el gen reportero de la luciferasa Amplite\u00ae, *Bright Glow*. Este kit utiliza una formulaci\u00f3n luminog\u00e9nica patentada para cuantificar la actividad de la luciferasa en c\u00e9lulas vivas y extractos celulares.<\/p>\n","protected":false},"featured_media":3213,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3212"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":4,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3212\/revisions"}],"predecessor-version":[{"id":3731,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3212\/revisions\/3731"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3213"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3212"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3212"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}