{"id":3173,"date":"2023-01-10T12:59:48","date_gmt":"2023-01-10T18:59:48","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3173"},"modified":"2023-01-20T14:16:40","modified_gmt":"2023-01-20T20:16:40","slug":"cell-meter-jc-10-mitochondrion-membrane-potential-assay-kit-optimized-for-flow-cytometry-assays","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cell-meter-jc-10-mitochondrion-membrane-potential-assay-kit-optimized-for-flow-cytometry-assays\/","title":{"rendered":"Cell Meter\u2122 JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*"},"content":{"rendered":"\n

Aunque JC-1 se usa ampliamente en muchos laboratorios, su poca solubilidad en agua causa inconvenientes extraordinarios. Incluso a una concentraci\u00f3n de 1 \u00b5M, JC-1 tiende a precipitar en un buffer acuoso. JC-10 est\u00e1 desarrollado para ser una alternativa superior a JC-1 donde se desea una alta concentraci\u00f3n de colorante. <\/p>\n\n\n\n

En comparaci\u00f3n con JC-1, JC-10 tiene una mejor solubilidad en agua . JC-10 es capaz de entrar selectivamente en las mitocondrias y cambia reversiblemente su color de verde a naranja a medida que aumentan los potenciales de membrana. Esta propiedad se debe a la formaci\u00f3n reversible de agregados JC-10 tras la polarizaci\u00f3n de la membrana que provoca cambios en la luz emitida de 520 nm (es decir, emisi\u00f3n de forma monom\u00e9rica JC-10) a 570 nm (es decir, emisi\u00f3n de agregado J). Cuando se excita a 490 nm, el color de JC-10 cambia reversiblemente de verde a naranja verdoso a medida que la membrana mitocondrial se vuelve m\u00e1s polarizada. Ambos colores se pueden detectar utilizando los filtros montados com\u00fanmente en todos los cit\u00f3metros de flujo, de modo que se puede analizar la emisi\u00f3n verde en el canal de fluorescencia 1 (FL1) y la emisi\u00f3n naranja verdosa en el canal 2 (FL2). Adem\u00e1s de su uso potencial en citometr\u00eda de flujo, tambi\u00e9n se puede utilizar en im\u00e1genes de fluorescencia y plataforma de microplaca de fluorescencia. <\/p>\n\n\n\n

Este kit proporciona todos los componentes esenciales con un m\u00e9todo de ensayo optimizado para la detecci\u00f3n de apoptosis en c\u00e9lulas con p\u00e9rdida del potencial de membrana mitocondrial. Este ensayo fluorom\u00e9trico se basa en la detecci\u00f3n de cambios en el potencial de la membrana mitocondrial en las c\u00e9lulas mediante el tinte cati\u00f3nico lipof\u00edlico JC-10. En c\u00e9lulas normales, JC-10 se concentra en la matriz mitocondrial donde forma agregados rojos fluorescentes. Sin embargo, en c\u00e9lulas apopt\u00f3ticas y necr\u00f3ticas, JC-10 existe en forma monom\u00e9rica y ti\u00f1e las c\u00e9lulas con fluorescencia verde. El kit est\u00e1 optimizado para la detecci\u00f3n de activadores e inhibidores de la apoptosis mediante citometr\u00eda de flujo. Tambi\u00e9n ofrecemos un pr\u00e1ctico kit de formato de placa de microtitulaci\u00f3n de fluorescencia de 96 y 384 pozos (Cat. No. \u00a022800) para la detecci\u00f3n de alto rendimiento.\u00a0 <\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22801<\/td>Cell Meter\u2122 JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays*<\/td>100 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n
\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Cit\u00f3metro de Flujo<\/mark> <\/p>\n\n\n\n

Excitaci\u00f3n laser<\/td>488 nm<\/td><\/tr>
Emisi\u00f3n filtro<\/td>530\/30 nm, 575\/26 nm<\/td><\/tr>
Especificaciones Instrumento<\/td>Canal FITC, PE <\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: 200X JC-10 in DMSO<\/td>1 vial (250 \u00b5L)<\/td><\/tr>
Componente B: Buffer de ensayo <\/td>1 botella (50 mL)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Espectro <\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>508<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>524<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
\n

PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

Agregue 50 \u00b5l de 200X JC-10 (componente A) en 5 ml de buffer de ensayo (componente B) y mezcle bien para preparar la soluci\u00f3n de trabajo con colorantes JC-10. Proteger de la luz.<\/em><\/p>\n\n\n\n

Para guias sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite<\/p>\n\n\n\n

https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n\n\n\n

<\/p>\n<\/div><\/div>\n\n\n\n

<\/div>\n\n\n\n

Imagen<\/mark><\/p>\n\n\n\n

\"\"
Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1<\/strong>. Efecto del cambio de potencial de membrana mitocondrial inducido por FCCP en c\u00e9lulas Jurkat. Las c\u00e9lulas Jurkat se cargaron con colorante JC-10 junto con DMSO (superior) o FCCP 5 \u00b5M (inferior) durante 10 minutos. Las intensidades de fluorescencia tanto para los agregados J como para las formas monom\u00e9ricas de JC-10 se midieron con un cit\u00f3metro de flujo FACSCalibur (Becton Dickinson) usando canales FL1 y FL2. Los datos no compensados \u200b\u200b(columna izquierda) se compararon con los datos compensados \u200b\u200b(columna derecha).<\/p>\n\n\n\n

<\/div>\n\n\n\n

Producto Alternativo<\/mark> <\/p>\n\n\n\n

Cell Meter\u2122 JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays*<\/a><\/p>\n\n\n\n

<\/div>\n\n\n\n

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<\/div>\n\n\n\n

Bibliograf\u00edas<\/mark><\/p>\n\n\n\n

Ver todas las 58 bibliograf\u00edas:\u00a0<\/em>Citation Explorer<\/a><\/p>\n\n\n\n

Impact of Organelle Transport Deficits on Mitophagy and Autophagy in Niemann–Pick Disease Type C<\/a>
Authors: <\/strong>Liedtke, Maik and V{\\”o}lkner, Christin and Hermann, Andreas and Frech, Moritz J
Journal: <\/strong>Cells (2022): 507<\/p>\n\n\n\n

Mitochondria Transfer from Adipose Stem Cells Improves the Developmental Potential of Cryopreserved Oocytes<\/a>
Authors: <\/strong>Kankanam Gamage, Udayanga Sanath and Hashimoto, Shu and Miyamoto, Yuki and Nakano, Tatsuya and Yamanaka, Masaya and Koike, Akiko and Satoh, Manabu and Morimoto, Yoshiharu
Journal: <\/strong>Biomolecules (2022): 1008<\/p>\n\n\n\n

Patchouli alcohol suppresses castration-resistant prostate cancer progression by inhibiting NF-$\\kappa$B signal pathways<\/a>
Authors: <\/strong>Cai, Jian and Zhao, Juan and Gao, Ping and Xia, Yuguo
Journal: <\/strong>Translational Andrology and Urology (2022): 528<\/p>\n\n\n\n

A comparative study of metformin and nicotinamide riboside in alleviating tissue aging in rats<\/a>
Authors: <\/strong>Geng, Lingling and Zhang, Bin and Liu, Haisong and Wang, Si and Cai, Yusheng and Yang, Kuan and Zou, Zhiran and Jiang, Xiaoyu and Liu, Zunpeng and Li, Wei and others,
Journal: <\/strong>Life Medicine (2022)<\/p>\n\n\n\n

DNA repair proteins as the targets for paroxetine to induce cytotoxicity in gastric cancer cell AGS<\/a>
Authors: <\/strong>Liu, Bang-Hung and Yuan, Tein-Ming and Huang, Chih-Jou and Hsu, Duan-Ting and Chen, Shi-Wen and Hsiao, Nai-Wan and Lin, Sheng-Chih and Wu, Shu-Wan and Lin, Yi-Mei J and Chuang, Show-Mei
Journal: <\/strong>American journal of cancer research (2022): 1465<\/p>\n\n\n\n

Effects of time-restricted feeding and type of food on fertility competence in female mice<\/a>
Authors: <\/strong>Konishi, Nafuko and Matsumoto, Hiroshi and Hashimoto, Shu and Gamage, Udayanga Sanath Kankanam and Tachibana, Daisuke and Fukuda, Aisaku and Morimoto, Yoshiharu and Koyama, Masayasu
Journal: <\/strong>Scientific Reports (2022): 1–15<\/p>\n\n\n\n

The relationship between free fatty acids and mitochondrial oxidative stress damage to trophoblast cell in preeclampsia<\/a>
Authors: <\/strong>Jiang, Lingling and Yan, Jianying
Journal: <\/strong>BMC Pregnancy and Childbirth (2022): 1–6<\/p>\n\n\n\n

Gracillin Isolated from Reineckia carnea Induces Apoptosis of A549 Cells via the Mitochondrial Pathway<\/a>
Authors: <\/strong>Yang, Jianqiong and Cao, Ling and Li, Yamei and Liu, Hai and Zhang, Minhong and Ma, Huamou and Wang, Biao and Yuan, Xiaoliang and Liu, Qian
Journal: <\/strong>Drug design, development and therapy (2021): 233<\/p>\n\n\n\n

Mitochondrial Function and Root-Filled Teeth–Detrimental and Unknown Interfaces in Systemic Immune Diseases<\/a>
Authors: <\/strong>Lechner, Johann and Mayer, Wolfgang
Journal: <\/strong>International Journal of General Medicine (2020): 387–402<\/p>\n\n\n\n

Inhibition of phosphodiesterase-10A by Papaverine protects human cortical neurons from quinolinic acid induced oxidative stress and synaptic proteins alterations<\/a>
Authors: <\/strong>Bhat, Abid and Tan, Vanessa and Heng, Benjamin and Essa, Musthafa M and Chidambaram, Saravana B and Guillemin, Gilles J
Journal: <\/strong>bioRxiv (2020)<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
Calcein<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Kit de ensayo JC-10 de potencial de membrana mitocondrial Cell Meter\u2122, *Optimizado para ensayos de citometr\u00eda de flujo*. Este ensayo fluorom\u00e9trico se basa en la detecci\u00f3n de cambios en el potencial de la membrana mitocondrial en las c\u00e9lulas mediante el tinte cati\u00f3nico lipof\u00edlico JC-10.<\/p>\n","protected":false},"featured_media":3175,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3173"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":5,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3173\/revisions"}],"predecessor-version":[{"id":3741,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3173\/revisions\/3741"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3175"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3173"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3173"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}