{"id":3147,"date":"2023-01-09T15:30:40","date_gmt":"2023-01-09T21:30:40","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3147"},"modified":"2023-01-20T16:41:00","modified_gmt":"2023-01-20T22:41:00","slug":"amplite-fluorimetric-lysyl-oxidase-assay-kit-red-fluorescence","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/amplite-fluorimetric-lysyl-oxidase-assay-kit-red-fluorescence\/","title":{"rendered":"Cell Meter\u2122 JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays*"},"content":{"rendered":"\n

Aunque JC-1 se usa ampliamente en muchos laboratorios, su poca solubilidad en agua dificulta su uso para algunas aplicaciones. Incluso a una concentraci\u00f3n de 1 \u00b5M, JC-1 tiende a precipitar en un buffer acuoso. JC-10 ha sido desarrollado para ser una alternativa superior a JC-1 donde se desea una alta concentraci\u00f3n de colorante. En comparaci\u00f3n con JC-1, nuestro JC-10 tiene una solubilidad en agua mucho mejor. JC-10 es capaz de entrar selectivamente en las mitocondrias y cambia reversiblemente su color de verde a naranja a medida que aumentan los potenciales de membrana. Esta propiedad se debe a la formaci\u00f3n reversible de agregados JC-10 tras la polarizaci\u00f3n de la membrana que provoca cambios en la luz emitida de 520 nm (es decir, emisi\u00f3n de forma monom\u00e9rica JC-10) a 570 nm (es decir, emisi\u00f3n de agregado J). Cuando se excita a 490 nm, el color de JC-10 cambia reversiblemente de verde a naranja verdoso a medida que la membrana mitocondrial se vuelve m\u00e1s polarizada. <\/p>\n\n\n\n

Este kit de ensayo de potencial de membrana mitocondrial Cell Meter\u2122 JC-10 le permite controlar los cambios de potencial de membrana mitocondrial mediante un lector de microplacas simple, mientras que todos los dem\u00e1s kits de ensayo JC-1 comerciales requieren el uso de un cit\u00f3metro de flujo. Nuestro kit proporciona el m\u00e9todo m\u00e1s s\u00f3lido para monitorear los cambios potenciales de la membrana mitocondrial y puede usarse f\u00e1cilmente para evaluar una gran biblioteca de compuestos.  Kit para 500 pruebas.<\/p>\n\n\n\n

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Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-22800<\/td>Cell Meter\u2122 JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays*<\/td>500 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

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Plataforma<\/mark><\/p>\n\n\n\n

Lector de Microplacas de Flourescencia<\/mark> <\/p>\n\n\n\n

Excitaci\u00f3n<\/td>490\/540 nm<\/td><\/tr>
Emisi\u00f3n<\/td>525\/590 nm<\/td><\/tr>
Cutoff<\/td>515\/570 nm<\/td><\/tr>
Placa recomendada<\/td>Pared negra \/ Fondo claro<\/td><\/tr>
Especificaciones Instrumento<\/td>Modo lectura inferioir<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Componentes<\/mark><\/p>\n\n\n\n

Componente A: 100X JC-10 in DMSO<\/td>1 vial (250 \u00b5L)<\/td><\/tr>
Componente B: Buffer de ensayo A<\/td>1 botella (25 mL)<\/td><\/tr>
Componente C: Buffer de ensayo B<\/td>1 botella (25 mL)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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Espectro <\/mark><\/p>\n\n\n\n

Abrir en Advanced Spectrum Viewer<\/a><\/em><\/p>\n\n\n\n

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Propiedades Espectrales<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n (nm)<\/td>508<\/td><\/tr>
Emisi\u00f3n (nm)<\/td>524<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
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PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

Agregue 50 \u00b5l de 100X JC-10 (componente A) en 5 ml de buffer de ensayo A (componente B) y mezcle bien para preparar la soluci\u00f3n de trabajo con colorantes JC-10. Proteger de la luz.<\/em><\/p>\n\n\n\n

Para guias sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite<\/p>\n\n\n\n

https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n<\/div><\/div>\n\n\n\n

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Imagen<\/mark><\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Figura 1<\/strong>. Los cambios en el potencial de membrana de las mitocondrias inducidos por camptotecina se midieron con JC-10\u2122 y JC-1 en c\u00e9lulas Jurkat. Despu\u00e9s de tratar las c\u00e9lulas Jurkat con camptotecina (10 \u00b5M) durante 4 horas, se a\u00f1adieron a los pozos las soluciones de trabajo con colorantes JC-1 y JC-10\u2122 y se incubaron durante 30 minutos. Las intensidades de fluorescencia tanto para los agregados JC-1 (resaltados en azul) como para las formas monom\u00e9ricas JC-10 (resaltadas en rojo) se midieron a Ex\/Em = 490\/525 nm (Cutoff = 515 nm) y 490\/ 590 nm (corte = 570 nm) con lector de microplacas NOVOstar (BMG Labtech).<\/p>\n\n\n\n

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Producto Alternativo<\/mark> <\/p>\n\n\n\n

Cell Meter\u2122 JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Flow Cytometry Assays*<\/a><\/p>\n\n\n\n

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Productos Relacionados<\/mark><\/p>\n\n\n\n

Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr>
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Cell Meter\u2122 Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Microplate Reader*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/mark><\/p>\n\n\n\n

Impact of Organelle Transport Deficits on Mitophagy and Autophagy in Niemann–Pick Disease Type C<\/a>
Authors: <\/strong>Liedtke, Maik and V{\\”o}lkner, Christin and Hermann, Andreas and Frech, Moritz J
Journal: <\/strong>Cells (2022): 507<\/p>\n\n\n\n

Antidepressants induce toxicity in human placental BeWo cells<\/a>
Authors: <\/strong>Nabekura, Tomohiro and Ishikawa, Shinya and Tanase, Makoto and Okumura, Taichi and Kawasaki, Tatsuya
Journal: <\/strong>Current Research in Toxicology (2022): 100073<\/p>\n\n\n\n

Mitochondria Transfer from Adipose Stem Cells Improves the Developmental Potential of Cryopreserved Oocytes<\/a>
Authors: <\/strong>Kankanam Gamage, Udayanga Sanath and Hashimoto, Shu and Miyamoto, Yuki and Nakano, Tatsuya and Yamanaka, Masaya and Koike, Akiko and Satoh, Manabu and Morimoto, Yoshiharu
Journal: <\/strong>Biomolecules (2022): 1008<\/p>\n\n\n\n

Patchouli alcohol suppresses castration-resistant prostate cancer progression by inhibiting NF-$\\kappa$B signal pathways<\/a>
Authors: <\/strong>Cai, Jian and Zhao, Juan and Gao, Ping and Xia, Yuguo
Journal: <\/strong>Translational Andrology and Urology (2022): 528<\/p>\n\n\n\n

DNA repair proteins as the targets for paroxetine to induce cytotoxicity in gastric cancer cell AGS<\/a>
Authors: <\/strong>Liu, Bang-Hung and Yuan, Tein-Ming and Huang, Chih-Jou and Hsu, Duan-Ting and Chen, Shi-Wen and Hsiao, Nai-Wan and Lin, Sheng-Chih and Wu, Shu-Wan and Lin, Yi-Mei J and Chuang, Show-Mei
Journal: <\/strong>American journal of cancer research (2022): 1465<\/p>\n\n\n\n

Effects of time-restricted feeding and type of food on fertility competence in female mice<\/a>
Authors: <\/strong>Konishi, Nafuko and Matsumoto, Hiroshi and Hashimoto, Shu and Gamage, Udayanga Sanath Kankanam and Tachibana, Daisuke and Fukuda, Aisaku and Morimoto, Yoshiharu and Koyama, Masayasu
Journal: <\/strong>Scientific Reports (2022): 1–15<\/p>\n\n\n\n

The relationship between free fatty acids and mitochondrial oxidative stress damage to trophoblast cell in preeclampsia<\/a>
Authors: <\/strong>Jiang, Lingling and Yan, Jianying
Journal: <\/strong>BMC Pregnancy and Childbirth (2022): 1–6<\/p>\n\n\n\n

Antineoplastic Effects and Mechanisms of a New RGD Chimeric Peptide from Bullfrog Skin on the Proliferation and Apoptosis of B16F10 Cells<\/a>
Authors: <\/strong>Jiang, Xuan and Zhang, Xin and Fu, Chao and Zhao, Ruili and Jin, Tianming and Liu, Mengyue and Pan, Chenhao and Li, Liu An and Ma, Jifei and Yu, Enyuan and others,
Journal: <\/strong>The Protein Journal (2021): 1–12<\/p>\n\n\n\n

Human VAMP3 Suppresses or Negatively Regulates Bax Induced Apoptosis in Yeast<\/a>
Authors: <\/strong>Akintade, Damilare D and Chaudhuri, Bhabatosh
Journal: <\/strong>Biomedicines (2021): 95<\/p>\n\n\n\n

Human VAMP3 Suppresses or Negatively Regulates Bax Induced Apoptosis in Yeast. Biomedicines 2021, 9, 95<\/a>
Authors: <\/strong>Akintade, DD and Chaudhuri, B
Journal: <\/strong>(2021)<\/p>\n\n\n\n

<\/div>\n\n\n\n

Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
Abbreviation of Common Chemical Compounds Related to Peptides<\/a>
Annexin V<\/a>
Bright Tide Fluor\u2122-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis<\/a>
Calcein<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Kit de ensayo Cell Meter\u2122 JC-10 de potencial de membrana mitocondrial *Optimizado para ensayos en microplaca*. Nuestro kit proporciona el m\u00e9todo m\u00e1s s\u00f3lido para monitorear los cambios potenciales de la membrana mitocondrial y puede usarse f\u00e1cilmente para evaluar una gran biblioteca de compuestos. <\/p>\n","protected":false},"featured_media":3161,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3147"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":11,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3147\/revisions"}],"predecessor-version":[{"id":3746,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3147\/revisions\/3746"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3161"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3147"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3147"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}