{"id":3117,"date":"2023-01-08T20:02:11","date_gmt":"2023-01-09T02:02:11","guid":{"rendered":"https:\/\/nuevo22.cidsamexico.com\/?post_type=al_product&p=3117"},"modified":"2023-01-20T16:45:58","modified_gmt":"2023-01-20T22:45:58","slug":"cell-meter-fluorimetric-intracellular-peroxynitrite-assay-kit-green-fluorescence-2","status":"publish","type":"al_product","link":"https:\/\/nuevo22.cidsamexico.com\/index.php\/productos\/cell-meter-fluorimetric-intracellular-peroxynitrite-assay-kit-green-fluorescence-2\/","title":{"rendered":"Cell Meter\u2122 Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*"},"content":{"rendered":"\n

El peroxinitrito (ONOO-) es una especie oxidante fuerte y un agente nitrante muy activo. El peroxinitrito se forma a partir de la reacci\u00f3n entre los radicales super\u00f3xido y el \u00f3xido n\u00edtrico generado en las c\u00e9lulas. Puede causar da\u00f1os a una amplia gama de biomol\u00e9culas, incluidas prote\u00ednas, enzimas, l\u00edpidos y \u00e1cidos nucleicos, lo que eventualmente contribuye a la muerte celular. Mientras tanto, el peroxinitrito tambi\u00e9n puede tener actividades protectoras in vivo al contribuir a las respuestas de defensa del hu\u00e9sped contra los pat\u00f3genos invasores. <\/p>\n\n\n\n

Por lo tanto, el peroxinitrito es un oxidante biol\u00f3gico esencial involucrado en una amplia gama de procesos fisiol\u00f3gicos y patol\u00f3gicos. Debido a su vida media extremadamente corta y su baja concentraci\u00f3n en estado estacionario, ha sido un desaf\u00edo detectar y comprender el papel del peroxinitrito en los sistemas biol\u00f3gicos. El DAX-J2\u2122 PON Green de AAT Bioquest se ha desarrollado para abordar esta necesidad insatisfecha. Proporciona una herramienta sensible para monitorear el nivel de ONOO en c\u00e9lulas vivas. DAX-J2\u2122 PON Green de AAT Bioquest reacciona espec\u00edficamente con ONOO- intercelular para generar un producto fluorescente de color verde brillante. Se puede utilizar en an\u00e1lisis de im\u00e1genes de fluorescencia, citometr\u00eda de flujo y lectores de microplacas de fluorescencia.<\/p>\n\n\n\n

<\/div>\n\n\n\n
Catalogo<\/th>Producto<\/th>Presentaci\u00f3n<\/th><\/tr><\/thead>
AAT-16315<\/td>Cell Meter\u2122 Fluorimetric Intracellular Peroxynitrite Assay Kit *Green Fluorescence*<\/td>100 pruebas<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n
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\"pdf\"\/<\/figure>\n\n\n\n

SDS<\/a><\/p>\n\n\n\n

\"pdf\"\/<\/figure>\n\n\n\n

Protocol<\/a><\/p>\n<\/div>\n\n\n\n

Importante: Solo para uso en investigaci\u00f3n (RUO). <\/p>\n\n\n\n

<\/div>\n\n\n\n

Plataforma<\/mark><\/p>\n\n\n\n

Microscopio de Fluorescencia <\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>490 nm<\/td><\/tr>
Emisi\u00f3n<\/td>530 nm<\/td><\/tr>
Placa recomendada<\/td>Pared negra \/ fondo claro<\/td><\/tr>
Especificaciones instrumento<\/td>Juego de filtro FITC<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

Lector de microplacas de Fluorescencia<\/mark><\/p>\n\n\n\n

Excitaci\u00f3n<\/td>490 nm<\/td><\/tr>
Emisi\u00f3n<\/td>530 nm<\/td><\/tr>
Cutoff<\/td>515 nm<\/td><\/tr>
Placa recomendada <\/td>Pared negra \/ fondo claro<\/td><\/tr>
Especificaciones Instrumento<\/td>Modo de lectura inferior<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

<\/p>\n\n\n\n

Componentes<\/mark><\/p>\n\n\n\n

Componente A: DAX-J2\u2122 PON Green<\/td>1 vial <\/td><\/tr>
Componente B: Buffer de ensayo<\/td>1 vial (1 mL\/vial)<\/td><\/tr>
Componente C: DMSO<\/td>1 vial (100 \u00b5L\/vial)<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

PREPARACION DE SOLUCION DE STOCK<\/p>\n\n\n\n

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A menos que se indique lo contrario, todas las soluciones madre no utilizadas deben dividirse en al\u00edcuotas de un solo uso y almacenarse a -20 \u00b0C despu\u00e9s de la preparaci\u00f3n. Evite los ciclos repetidos de congelaci\u00f3n y descongelaci\u00f3n.<\/em><\/p>\n\n\n\n

Soluci\u00f3n madre DAX-J2\u2122 PON Green (500X):<\/em>
Agregue 20 \u00b5L de DMSO (Componente C) en el vial de DAX-J2\u2122 PON Green (Componente A) y mezcle bien. Nota: 20 \u00b5L de soluci\u00f3n madre de DAX-J2\u2122 PON Green reconstituida es suficiente para 1 placa.<\/p>\n<\/div><\/div>\n\n\n\n

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PREPARACION DE SOLUCION DE TRABAJO<\/p>\n\n\n\n

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Agregue 10 \u03bcL de soluci\u00f3n madre del sensor de peroxinitrito DAX-J2\u2122 reconstituido con DMSO 500X en 500 \u03bcL de buffer de ensayo (componente B) y mezcle bien. Nota:<\/em> La soluci\u00f3n de trabajo no es estable; prep\u00e1relo seg\u00fan sea necesario antes de usarlo.<\/p>\n\n\n\n

Para guia sobre la preparaci\u00f3n de muestras de c\u00e9lulas, visite:<\/p>\n\n\n\n

https:\/\/www.aatbio.com\/resources\/guides\/cell-sample-preparation.html<\/a><\/p>\n<\/div><\/div>\n\n\n\n

<\/div>\n\n\n\n

Imagenes<\/mark><\/p>\n\n\n\n

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Fig. 1<\/figcaption><\/figure>\n\n\n\n

Figura 1<\/strong>. Im\u00e1genes de fluorescencia de peroxinitrito intracelular en c\u00e9lulas macr\u00f3fagas RAW 264.7 utilizando el kit de ensayo de peroxinitrito intracelular fluorim\u00e9trico Cell Meter\u2122 (n.\u00b0 de cat. 16315). Se sembraron c\u00e9lulas 264.7 sin procesar a 100.000 c\u00e9lulas\/pocillo\/100 \u00b5l durante la noche en una placa de 96 pocillos de pared negra\/fondo transparente Costar. Tratamiento SIN-1: las c\u00e9lulas se coincubaron con DAX-J2\u2122 PON Green y SIN-1 100 \u00b5M a 37 \u00b0C durante 1 hora. Control sin tratar: las c\u00e9lulas RAW 264.7 se incubaron con DAX-J2\u2122 PON Green sin tratamiento con SIN-1. Las se\u00f1ales de fluorescencia se midieron utilizando un microscopio de fluorescencia con un filtro FITC<\/p>\n\n\n\n

<\/div>\n\n\n\n
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Fig. 2<\/figcaption><\/figure>\n\n\n\n

Figura 2.<\/strong> Detecci\u00f3n de peroxinitrito en c\u00e9lulas vivas tras el tratamiento con SIN-1 utilizando el kit de ensayo de peroxinitrito intracelular fluorim\u00e9trico Cell Meter\u2122 (n.\u00b0 de cat\u00e1logo 16315). Se sembraron c\u00e9lulas RAW 264.7 a 100.000 c\u00e9lulas\/pocillo\/100 \u00b5l durante la noche en una placa de 96 pocillos Costar de pared negra\/fondo transparente. Las c\u00e9lulas se coincubaron con la soluci\u00f3n de trabajo DAX-J2\u2122 PON Green y SIN-1 a una concentraci\u00f3n de 50 a 200 \u00b5M a 37 \u00baC durante 1 hora. Las c\u00e9lulas incubadas con DAX-J2\u2122 PON Green sin tratamiento con SIN-1 se usaron como control. La se\u00f1al de fluorescencia se control\u00f3 a Ex\/Em = 490\/530 nm (corte = 515 nm) con modo de lectura inferior utilizando un lector de microplacas FlexStation (Molecular Devices).<\/p>\n\n\n\n

<\/div>\n\n\n\n
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Fig. 3<\/figcaption><\/figure>\n\n\n\n

Figura 3.<\/strong> Medici\u00f3n del lector de microplacas de c\u00e9lulas RAW 264.7 marcadas con (A) DAX-J2 PON Green o (B) DHR 123. Las c\u00e9lulas RAW 264.7 se trataron con diferentes concentraciones de SIN-1. Se us\u00f3 Ebselen a una concentraci\u00f3n de 20 \u00b5M como eliminador de ONOO. En comparaci\u00f3n con DHR 123, el ebselen inhibi\u00f3 m\u00e1s completamente el aumento de la fluorescencia de las c\u00e9lulas marcadas con DAX-J2 PON Green tras el tratamiento con SIN-1. Como en hallazgos anteriores, la oxidaci\u00f3n de DHR 123 en cualquier tipo de c\u00e9lula puede involucrar no solo a ONOO- sino tambi\u00e9n a otras ROS\/RNS relacionadas. Estos resultados resaltan a\u00fan m\u00e1s la alta selectividad de DAX-J2 PON Green para la detecci\u00f3n de ONOO intracelular.<\/p>\n\n\n\n

<\/div>\n\n\n\n

Productos Relacionados<\/mark><\/p>\n\n\n\n

Nombre<\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular pH Assay Kit<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Peroxynitrite Assay Kit *Optimized for Flow Cytometry*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Blue Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Intracellular Fluorimetric Hydrogen Peroxide Assay Kit *Green Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Mitochondrial Hydroxyl Radical Detection Kit *Red Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Mitochondrial Superoxide Activity Assay Kit *Green Fluorescence*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Microplate Reader*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Orange Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Fluorimetric Intracellular Nitric Oxide (NO) Activity Assay Kit *Red Fluorescence Optimized for Flow Cytometry*<\/a><\/td><\/tr>
Cell Meter\u2122 Live Cell Caspase 3\/7 Binding Assay Kit *Green Fluorescence*<\/a><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n
<\/div>\n\n\n\n

Bibliograf\u00eda<\/mark><\/mark><\/mark><\/p>\n\n\n\n

Triterpenoids and ultrasound dual-catalytic nanoreactor ignites long-lived hypertoxic reactive species storm for deep tumor treatment<\/a>
Authors: <\/strong>Li, Ziying and Xie, Huanzhang and Shi, Huifang and Li, Dongmiao and Zhang, Zizhong and Chen, Haijun and Gao, Yu
Journal: <\/strong>Chemical Engineering Journal (2023): 139938<\/p>\n\n\n\n

Interactions between pH, reactive species, and cells in plasma-activated water can remove algae<\/a>
Authors: <\/strong>Mizoi, Ken and Rodr{\\’\\i}guez-Gonz{\\’a}lez, Vicente and Sasaki, Mao and Suzuki, Shoki and Honda, Kaede and Ishida, Naoya and Suzuki, Norihiro and Kuchitsu, Kazuyuki and Kondo, Takeshi and Yuasa, Makoto and others,
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Mechanisms of oxidative removal of 1, 4-dioxane via free chlorine rapidly mixing into monochloramine: Implications on water treatment and reuse<\/a>
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Peroxynitrite (ONOO-) generation from the HA-TPP@ NORM nanoparticles based on synergistic interactions between nitric oxide and photodynamic therapies for elevating anticancer efficiency<\/a>
Authors: <\/strong>Jiang, Dawei and Yue, Tao and Wang, Guichen and Wang, Chaochao and Chen, Chao and Cao, Hongliang and Gao, Yun
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Nitric oxide and reactive oxygen species-releasing polylactic acid monolith for enhanced photothermal therapy of osteosarcoma<\/a>
Authors: <\/strong>Lee, Ji-Hye and Uyama, Hiroshi and Kwon, Oh-Kyoung and Kim, Young-Jin
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Fluorescent real-time quantitative measurements of intracellular peroxynitrite generation and inhibition<\/a>
Authors: <\/strong>Luo, Zhen and Zhao, Qin and Liu, Jixiang and Liao, Jinfang and Peng, Ruogu and Xi, Yunting and Diwu, Zhenjun
Journal: <\/strong>Analytical biochemistry (2017): 44–48<\/p>\n\n\n\n

Gene silencing of endothelial von Willebrand Factor attenuates angiotensin II-induced endothelin-1 expression in porcine aortic endothelial cells<\/a>
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<\/div>\n\n\n\n

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Application Notes (en Ingles)<\/mark><\/p>\n\n\n\n

A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells<\/a>
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells<\/a>
A Meta-Analysis of Common Calcium Indicators<\/a>
A New Red Fluorescent & Robust Screen Quest\u2122 Rhod-4\u2122 Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets<\/a><\/p>\n\n\n\n

<\/div>\n","protected":false},"excerpt":{"rendered":"

Kit de ensayo de peroxinitrito intracelular fluorim\u00e9trico Cell Meter\u2122 *Fluorescencia verde*. AAT Bioquest ha desarrollado DAX-J2\u2122 PON Green, herramienta sensible para monitorear el nivel de peroxinitrito (ONOO-) en c\u00e9lulas vivas. <\/p>\n","protected":false},"featured_media":3123,"template":"","al_product-cat":[34],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3117"}],"collection":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product"}],"about":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/types\/al_product"}],"version-history":[{"count":11,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3117\/revisions"}],"predecessor-version":[{"id":3598,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product\/3117\/revisions\/3598"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media\/3123"}],"wp:attachment":[{"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/media?parent=3117"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"https:\/\/nuevo22.cidsamexico.com\/index.php\/wp-json\/wp\/v2\/al_product-cat?post=3117"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}